• The Journal of Korean Physical Therapy
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• v.9 no.1
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• pp.71-79
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• 1997

The purpose of this study were to determined the effect of laser irradiation on the fibroblast activity. Cultures of 3T3 fibroblasts were subjected to Helium Neon laser(632.8 nm) irradiation of various energy density. On one, two and three consecutive days the fibroblast monolayers wert irradiated for period from 0 to 32 minutes with 8 mW of average output power. The fibroblast activity was determined by the quantitative assay of MTT, SRB and NR after incubation of the fibroblasts for 24 hours. Results show that exposure duration from 2 min to 32 min could increase MTT at three consecutive days, whereas control and 1 min, one and two days irradiation had were not inclosed. The SRB and NR were inclosed at two and three consecutive days from 2 min to 32 min, whereas control and 1 min, and once radiation were not increased. These result demonstrate that energy density from 0.48 to 7.64 J/cm could increase cellular protein contents and fibroblast activity at more than twice irradiation of laser, whereas low energy density (less than 0.24 J/m) and once irradiation of laser had no effect. The results suggest that the beneficial effect of the He-Ne laser with adequate dose on fibroblast activity in vitro.

• The Korean Journal of Pain
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• v.6 no.2
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• pp.185-191
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• 1993

• The Journal of Korean Medicine
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• v.26 no.4
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• pp.143-151
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• 2005

Objectives: The purpose of this study was to investigate the effect of exterior vascular laser irradiation therapy by live blood analysis. Methods: We analysed the changing forms of the live blood sample with a microscope before and after exterior vascular laser irradiation therapy of the blood. Results: Rouleau of red cells, erythrocyte aggregation of red cells, thrombocyte aggregation, uric acid crystals, red crystals, and protoplasts in blood were decreased significantly after exterior vascular laser irradiation therapy. Conclusions: This study suggests that exterior vascular laser irradiation will have positive effects in eliminating various effete matters in blood and will have efficacy in the treatment and prevention of cardiovascular system disorders and hyperlipidemia, caused by effete matters, or numbness and arthralgia caused by blood stagnancy and blood circulation disorder.

• Journal of Dental Anesthesia and Pain Medicine
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• v.19 no.3
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• pp.159-165
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• 2019

Background: Dental procedures commonly involve the injection of local anesthetic agents, which causes apprehension in patients. The objective of dental practice is to provide painless treatment to the patient. The purpose of this study was to evaluate the effect of Low Level Laser Therapy (LLLT) in reducing the pain due to local anesthetic injection. Materials and Methods: A prospective, split-mouth study was conducted on 25 patients. In Condition A, LLLT was administered followed by the administration of a standard local anesthetic agent. Patients' perception of pain with use of LLLT was assessed based on a Visual Analogue Scale (VAS). In Condition B, LLLT was directed to the mucosa but not activated, followed by the administration of local anesthesia. VAS was used to assess the pain level without the use of LLLT. Results: Comparison between Condition A and Condition B was done. A P value < 0.001 was considered significant, indicating a definite statistical difference between the two conditions. Conclusion: In our study, we observed that LLLT reduced pain during injection of local anesthesia. Further multi-centric studies with a larger sample size and various modifications in the study design are required.

• The Journal of Korean Physical Therapy
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• v.12 no.3
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• pp.469-474
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• 2000

This studies were to investigate the effects of low power intensity Helium-Neon Infra-Red(He-Ne IR) laser on the changes of the serum immunoglobulin(1g) components in burn rats, The fourty eight Spraque-Dawley adult male rats were assigned to the 8 groups: the experimental groups(4), the burn control groups(2) and the control groups(2) There was made three degree burn by the 250mW IR on the back of each rats, from 3 days after being burned, the experimental laser groups were irradiated low power He-Ne IR laser for 3 minutes and 4.5 minutes every day during the 7days and 21 days. The results were as follows: The concentrations of immunoglobulin G(Ig G) in serum of burn rats on the treated with during the 3minutes laser for 7 days were higher than those of burn and 4.5 minutes laser groups. There were significantly decreased the change of the level of immunoglobulin M(Ig M) in serum of burn rats on the treated with 3 minutes laser for 7 days to the control group, but were significantly increased on the 3minutes and 4.5 minutes laser groups for 21 days to the those of burn and control groups, and the levels of the Ig M on the 3minutes laser group for 21 days were higher than those of 7 days. The concentrations of immunoglobulin E(Ig E) in serun of the burn rats on the experimental and burn groups were significantly lower than those in control group. As above results, The changes of the level of immonoglobulin components in serum have meaningful role in the healing process on the treated with the low pewer intensity of HE-Ne IR laser.

• Journal of Periodontal and Implant Science
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• v.26 no.1
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• pp.230-243
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• 1996

Root surface exposure due to gingival recession after periodontal surgery, dentin exposure after root planing elicit pain response when exposed to mechanical, heat, chemical or osmotic stimulation. Especially, patients treated with periodontal surgery, show high frequency and there have been reports showing the 1 out of 7 patients have dentin hypersensitivity. There have been many studies on the clinical effects of various materials on the treatment of dentin hypersensitivity. but, none could provide absolute clinical efficacy. In this study, 45 teeth from 30 patients, who had had periodontal surgery and showed dentin hypersensitivity after surgery were chosen for the experimental group and they were illuminated with laser, 15teeth were chosen for the control group and they were not exposed to laser. After this dentin hypersensitivity was elicited by tactile, compressed air, cold water and then, the degree was evaluated using NRS(Numerical Rating Scale). And during LLLT(Low Level Laser Therapy) semiconductor laser using Gallium - Arsenide as a diode was illuminated for 180 seconds at a frequency of 7(500Hz). This therapy was done 10 times, and each time the changes in dentin hypersensitivity was evaluated using NRS. The results were as follows : 1. After treat with LLLT on dentin hypersensitivity due to periodontal surgery, 22.2% showed total loss of dentin hypersensitivity, 60.0% showed loss of tactile dentin hypersensitivity, 48.8% showed loss of compressed air dentin hypersensitivity, 22.2% showed loss of cold water dentin hypersensitivity. 2. As a result of clinical evaluation of dentin hypersensitivity using NRS, there was significant increase in improvement of dentin hypersensitivity in the experimental group compare to the control group(P<0.05). And there was almost no natural loss of dentin hypersensitivity in the control group. 3. In comparison of the stages of evaluation, there was significant difference in between experimental and control group. after the second visit(P<0.05), and the difference increased with each visit.

• Journal of Oral Medicine and Pain
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• v.21 no.1
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• pp.25-36
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• 1996

To investigate the effect of low level laser therapy, the author selected 37 dental students with tender points in both masseters and trapeziuses, also measured maximum comportable opening(MCO), Numerical analog scale(NAS) and pressure pain threshold (PPT). 20 subjects were assigned randomly and were treated with GaAlAs diode laser after ultrasound. The other 17 subjects were treated with ultrasound and laser without irradiation. All the subjects were treated after 2 and 4 day respectively and were examined again after 6 days. And the obtained results were as follows : 1. The MCO of irradiated group increased more significantly after treatment than non-irradiated group. 2. The NAS of irradiated group decreased more significantly after treatment than non-irradiated group. 3. The PPT or irradiated group increased more significantly after treatment than non-irradiated group.

• Journal of Dental Anesthesia and Pain Medicine
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• v.19 no.5
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• pp.289-294
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• 2019

Background: Pain, limitations in opening, asymmetrical jaw movements, and temporomandibular joint (TMJ) sounds are the most common findings in temporomandibular joint disorders (TMDs), which causes excruciating pain, inflammation of the surrounding muscles, posterior fibers, and synovial fluid. This study aimed to evaluate and compare the effects of ultrasound heat therapy and low-level laser therapy (LLLT) in reducing TMD-related pain. Methods: This prospective study included 42 patients (age range, 25-45 years), who were divided into two groups of 21 patients each. All patients were prescribed a non-steroidal anti-inflammatory drug (NSAID) twice a day for 5 days for temporary relief of pain prior to the commencement of treatment. Patients were kept on a soft diet and asked to restrict mouth opening during the same period. Fifteen sessions of LLLT (Group A) or ultrasound therapy (Group B) were administered to the affected side. Results: Post-therapy, the mean visual analog scale score for group A and group B was 4.81 (2.01) and 6.19 (1.20), respectively; the difference was statistically significant and favoring the LLLT group. Similarly, the mean mouth opening for group A and group B was 3.99 (0.40) and 3.65 (0.41), respectively; the difference was statistically significant and favoring the LLLT group. Conclusion: Our study recommends LLLT for treating TMD-related pain with no underlying bony pathology.

• Biomedical Science Letters
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• v.13 no.3
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• pp.223-230
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• 2007

Low power laser and neuromuscular electrical stimulation have been utilized in many clinical applications for the treatment of musculoskeletal lesion. This study was to investigate the effects of low power He-Ne IR laser and neuromuscular electrical stimulation on the change of the serum biochemical components in experimentally induced muscle injured rats. The twenty Sprague-Dawley male rats were randomly assigned to the 4 groups: the normal group (n=5), the control group (n=5), the low power laser irradiation group (LLI) (n=5) and the neuromuscular electrical stimulation group (NMES) (n=5). The experimentally induced muscle injury was induced by the subcutaneous injection of a 0.1 ml Freund's Complete Adjuvant into the right hind paw and calf muscles in control, LLI and NMES groups. The LLI group was irradiated with He-Ne IR laser for 5 minutes every day during 10 days. Also, the NMES group was electrically stimulated 15 minutes every day during 10 days. The normal and control groups were not received with any therapeutic intervention. The authors performed tests which were the concentration of the serum biochemical components to detect the effects of therapeutic interventions. The results were as follows: 1. The level of the aspartate aminotransferase (AST) was significantly decreased in the LLI and NMES groups compare to the control group. 2. The level of the serum lactate dehydrogenase (LDH) was significantly decreased in the LLI and NMES groups compare to the control group. 3. The level of the serum creatine kinase (CK) was significantly decreased in the LLI and NMES groups compare to the control group. From the results, we could come to the conclusion that low power laser and neuromuscular electrical stimulation could be accelerating healing processes of skeletal muscle injury and further clinical work will be required.

• Medical Lasers
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• v.10 no.1
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• pp.37-44
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• 2021

Background and Objectives Hypothyroidism is the most common endocrine disease. On the other hand, there is no treatment that can improve the thyroid function. Low-level laser therapy (LLLT) can improve the cellular activity. The effect of hypothyroidism is not obvious. This study examined the effects of LLLT on the thyroid gland function of a propylthiouracil (PTU)-induced hypothyroidism mouse model. Materials and Methods Twenty-five male ICR mice were distributed into five groups of five animals each: Negative control (none PTU animal) and positive control (PTU animal) of unirradiated animals, and three experimental groups with LLLT (3J, 6J, and 12J). Each mouse was exposed to a distinct dose of a 632-nm laser once a week for three rounds. The positive control group and three LLLT groups were induced into a hypothyroidism state by PTU administration. The animals' thyroid-stimulating hormone and thyroxine levels were measured using an ELISA kit, and their thyroid tissue was harvested and analyzed after sacrifice at the end of the experiment. The hormone level and morphological changes in the tissue of the five groups were compared. Results The thyroid hormone levels in the control group and LLLT groups were similar. On the other hand, the thyroid tissue of the LLLT groups showed some morphological changes that were similar to those of iodine deficiency thyroid. Conclusion LLLT did not affect the thyroid gland function in PTU-induced hypothyroidism mice.