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Study of Ancient traditional medical text Lisangsokdanbang through the Uibangyuchwi (『의방유취(醫方類聚)』에 인용된 『이상속단방(理傷續斷方)』의 서지연구)

  • Kim, Young Gon;Kim, Nam Il;Ahn, Sang Woo
    • The Journal of Korean Medical History
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    • v.20 no.1
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    • pp.22-29
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    • 2007
  • Lisangsokdanbang is the oldest known medical document in existence. It is, however lost-its original copy could not be traced. But the contents of Lisangsokdanbang quoted in Uibangyuchwi, a document published in Chosun in 1447. Thus this study aims to restore the contents of Lisangsokdanbang based on Uibangyuchwi and present the synthesis of facts on Lisangsokdanbang extracted from the reference books.

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The Bodongbiyo Medical Stuffs Quoted in Uibangyuchwi (『의방유취(醫方類聚)』에 인용된 『보동비요(保童秘要)』의 본초(本草) 고찰을 통해 본 조선(朝鮮) 전기(前期) 소아의학(小兒醫學))

  • Lee, Gha Eun;Ahn, Sang Woo
    • The Journal of Korean Medical History
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    • v.20 no.1
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    • pp.30-40
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    • 2007
  • Uibanyuchwi, published in 1477, includes the contents of many lost ancient Korean and Chinese medicine documents. Through studying Uibanyuchwi, the ancient Korean pediatry medical document Bodongbiyo could be restored. Bodongbiyo was Korea's representative medical document of the pediatry field that was used until early Chosun dynasty. It was influenced by China's Big medical text, Taepyungseonghaebang and many mineral and animal medical stuffs are listed there. This was influenced by its contemporary factors.

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Antibacterial Activity and Characteristics of Bacteriocin Produced by Lactobacillus plantarum LMG 7945 (Lactobacillus plantarum LMG 7945가 생산하는 bacteriocin의 항균력과 그 특성)

  • 김상현;이명숙;장동석
    • Journal of Food Hygiene and Safety
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    • v.10 no.2
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    • pp.65-71
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    • 1995
  • Bacteriocins from lactic acid bacteria have attracted much attention in recent years because of their useful worth in increasing safety and extending shelf life of foods. These substances show an inhibitory effect against some food spoilage bacteria and food-borne pathogens. The inhibitory effect fo the bacteriocin produces by lactic acid bacteria against Listeria monocytogenes(L. monocytogenes) was examined in this study. The culture supernatants of 5 kinds of bacteria among the 10 kinds of testes lactic acid bacteria had the inhibitory activity against Listeria sp., various Gram positive and Gram negative bacteria. Bacteriocin produced by Lactobacillus plantarum(Lact. plantarum) LMG 7945 was the most active toward L. monocytogenes. Bacteriocin production of the Lact. plantarum LMG 7945 cultured on MRS broth was increased late logarithmic phase over early stationary phase. This bacteriocin was stable at heat treatment and acidic pH relatively; The activity was retained after heating at 121$^{\circ}C$ for 15min and was active in the pH range of 2~4 but was lost above pH 5.

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Stability of Sweet Potato $\beta$Amylase (I) (고구마 $\beta$아밀라아제의 안정성에 관한 연구 (1))

  • 안용근;이석건
    • The Korean Journal of Food And Nutrition
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    • v.9 no.3
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    • pp.247-252
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    • 1996
  • $\beta$-Amylase was purified from sweet potato by acetone fractlonatlon, Sephadex A-50 ion exchange chromatography and Sepgadex G-200 gel chromatographyl The higher enzyme concentration was, the higher heat stability of enzyme became. After 1 hour 30 minute. At 6$0^{\circ}C$ in pH 5, enzyme under concentration of 30$\mu$l/ml lost its activity completely and over the concentration of 100$\mu$g/ml remained 25% of activity. The enzyme was stabilized at range of pH 4~10 and pH stability was increased by glycerol. Five moles of NaCl inhibited completely of the enzyme activity. SDS of 0.05% inhibited the enzyme completely after 12 hours at 37$^{\circ}C$ in pH5. One mole guanidine-HCl and 8M urea inhibited the entire enzyme after 13 hours at 37$^{\circ}C$ in pH 5.

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The Extraction of Alignment Information on Railroad Using Digital Imagery (디지털 영상을 이용한 철도선형정보 추출)

  • Lee Jong-Chool;Cha Sung-Yeol;Kim Jin-Soo;Nam Il-Hyun
    • Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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    • 2006.04a
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    • pp.271-276
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    • 2006
  • Railroads made many contribution to modernization being developed so quickly as public transportation, but after middle of 20 century, its qualities are getting low because of focusing on the investment and development of cars and airplanes. Its role which is getting the excellent merits on the mass transportation, rapid transit. safety, state period, energy efficiency, and prevention of environmental pollution has been reconsidered. Element of horizontal alignment In an existing railroad line is needed as basic materials, when it will be improved or moved. If its design drawing was lost or damaged, it is impossible to recover. It is riot easy to repair for it as disasters. We must understand an existing railroad line to bring a function included a basic geography situation. In this studies we acquire, analyze. and handle the digital picture of railroads and then reappear shape of three dimension. But also we want to contribute to establish the facilities information choosing materials of existing railroad line in outputting the alignment element by reverse.

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Optimization of Refolding Conditions for the Aklavinone 11-Hydroxylase of Streptomyces peucetius Overexpressed in Escherichia coli. (대장균에서 대량 발현된 Streptomyces peucetius유래 Aklavinone 11-Hydroxylase효소의 최적 가용화 조건)

  • 민우근;홍영수;최용경;이정준;홍순광
    • Microbiology and Biotechnology Letters
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    • v.26 no.4
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    • pp.365-368
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    • 1998
  • The aklavinone 11-hydroxylase which was overexpressed using T7 promoter in E. coli could be detected in SDS-PAGE only in insoluble precipitate without any detectable enzyme activity. The insoluble enzyme was solubilized in 6M guanidine$.$HCl solution and their refolding ability was tested under various conditions. When the enzymatic activity was checked by the bioconversion experiment, stepwise dialysis against 6M, 3M, 1M guanidine$.$HCl and finally 100 mM potassium phosphate buffer of the solubilized protein gave the best bioconversion efficiency. The aklavinone 11-hydroxylase showed its enzymatic activity in the reaction buffer containing NADPH with vigorous shaking. The enzymatic activity was lost during partial purification and regained by the addition of crude extract of S. lividans in the reaction mixture. This effect was confirmed to due to some low-molecular weight component(s) in the crude extract, because the addition of dialyzed crude extract could not recover the enzymatic activity.

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Torque Characteristics of Cam/Tappet System (엔진 캠/태핏 시스템의 작동토크 측정과 마찰특성)

  • 여창동;김대은;윤정의
    • Transactions of the Korean Society of Automotive Engineers
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    • v.6 no.5
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    • pp.66-74
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    • 1998
  • The operating torque and tribological characteristics of a cam/tappet system of an I.C. engine have an important effect on the engine efficiency. More power is lost for higher operation torque which is affected by the friction of a cam/tappet system. In this work experimental investigation of the torque behavior of a cam/tappet system was conducted to get tribological characteristics. Specifically, the torque was measured with respect to oil temperature and camshaft speed. The torque decreased with increasing camshaft speed because of decreasing friction coefficient but was hardly affected by the oil temperature. Also, the torque was the largest near the cam nose region.

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Malonate Metabolism: Biochemistry, Molecular Biology, Physiology, and Industrial Application

  • Kim, Yu-Sam
    • BMB Reports
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    • v.35 no.5
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    • pp.443-451
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    • 2002
  • Malonate is a three-carbon dicarboxylic acid. It is well known as a competitive inhibitor of succinate dehydrogenase. It occurs naturally in biological systems, such as legumes and developing rat brains, which indicates that it may play an important role in symbiotic nitrogen metabolism and brain development. Recently, enzymes that are related to malonate metabolism were discovered and characterized. The genes that encode the enzymes were isolated, and the regulation of their expression was also studied. The mutant bacteria, in which the malonate-metabolizing gene was deleted, lost its primary function, symbiosis, between Rhizobium leguminosarium bv trifolii and clover. This suggests that malonate metabolism is essential in symbiotic nitrogen metabolism, at least in clover nodules. In addition to these, the genes matB and matC have been successfully used for generation of the industrial strain of Streptomyces for the production of antibiotics.

Comparison of vibration and noise of higher utility factor Insulation Panel for Transformer (변압기용 고효율 차음판의 진동 및 소음 비교)

  • Jeong, J.H.;Lee, J.H.;Jeong, H.E.;Lim, D.S.;Kim, J.;Choi, B.K.
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2007.11a
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    • pp.112-116
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    • 2007
  • Highly damped insulation panel for transformer construction is needed in residential area because the making noise from transformer substation in inner city is appeared a lost of problem by increasing to conserve living environment. Therefor in this paper, the vibration and noise characteristic effect of cork-type and sponge-type that is attached between insulation panel and enforce beam in transformer is analized using real size transformer experimentally.

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A Case of Rapunzel Syndrome

  • Kim, Joon Sung;Nam, Chang Woo
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.16 no.2
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    • pp.127-130
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    • 2013
  • Rapunzel syndrome refers to a very rare condition in which swallowed hair forms a gastric trichobezoar that has a long tail extending into the small bowel. We describe a case of Rapunzel syndrome in an 8-year-old girl who presented with abdominal mass, epigastric pain and vomiting. Abdominal computed tomography scan showed a markedly dilated stomach filled with coarse heterogeneous materials. Upper gastrointestinal endoscopy revealed a huge hairy ball with a tail extending through the pylorus. We performed a surgical laparotomy and successfully removed a huge trichobezoar with a long tail extending into the middle portion of jejunum. Psychiatric consultation with review showed her past history of trichotillomania and trichophagia 4 years ago. But her parents denied further psychiatric therapy and she was lost to the follow-up. Rapunzel syndrome should be included in the differential diagnosis in children with chronic abdominal pain and trichophagia.