• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.87-91
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• 2005

Although 2D-PAGE has been widely used as the primary method for protein separation, difficulties in displaying proteins with an extreme values of isoelectric paint (pI), molecular size and hydrophobicity limit the technique. In addition, time consuming steps involving protein transfer and extraction from the gel-pieces can result in sample loss. Here, we describe a novel protein separation technique with capillary size-exclusion chromatography (CSEC) for rapid protein identification from human plasma. The method includes protein fractionation along with molecular size followed by in-solution tryptic digestion and peptide analysis through reversed phase liquid chromatography (RPLC) coupled to nanoflow electrospray-tandem mass spectrometry (ESI-MS/MS). Tryptic peptides are applied an a $100\;{\mu}m\;i.d.{\times}10mm$ length pre-column and then separated on a $75\;{\mu}m{\times}200mm$ analytical column at -100 nL/min flaw rate. Proteins were identified over the wide ranges of pI (3.7-12.3) when this technique was applied to the analysis of $1-2\;{\mu}L$ of human plasma. This gel-free system provides fast fractionation and may be considered a complementary technique to SDS-PAGE in proteomics.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.11
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• pp.2015-2021
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• 2017

Recently, a lot of attention is fallen to IoT(Internet of Things) for hyper-connected society and the number of unlicensed wireless device has been increasing. Thus, this paper analyzed the impact of unlicensed wireless device on the basis of 802.11ah on licensed LTE user equipment in 900 MHz frequency band for efficient frequency use. As the interference analysis method, Minimum Coupling Loss (MCL) method and Monte Carlo (MC) method were used. In case of one interferer, minimum separation distance between interferer and victim was calculated as about 22 m through the MCL method under the assumption of the worst case. The maximum number of interferer to meet the interference probability of 5% below within a cell radius of the victim was computed as about 3000 by using MC method based on statistical technique. The analysis method and results in this paper are expected to be used for the coexistence between unlicensed wireless device and licensed wireless device.

• ETRI Journal
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• v.33 no.2
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• pp.279-282
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• 2011

Coexistence analysis is extremely important in examining the possibility for spectrum sharing between orthogonal frequency-division multiplexing (OFDM)-based international mobile telecommunications (IMT)-Advanced and other wireless services. In this letter, a new closed form method is derived based on power spectral density analysis in order to analyze the coexistence of OFDM-based IMT-Advanced systems and broadcasting frequency modulation (FM) systems. The proposed method evaluates more exact interference power of IMT-Advanced systems in FM broadcasting systems than the advanced minimum coupling loss (A-MCL) method. Numerical results show that the interference power is 1.3 dB and 3 dB less than that obtained using the A-MCL method at cochannel and adjacent channel, respectively. This reduces the minimum separation distance between the two systems, which eventually saves spectrum resources.

• Journal of computational fluids engineering
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• v.21 no.2
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• pp.40-46
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• 2016

In the paper, a study on the analysis of fouling effect of the venturi flowmeter is described. In the research flow field solutions about the venturi flowmeter with fouling are obtained and then the effects on fouling states by inserting a ring into the throat of venturi flowmeter are studied. As the result shows, it is found that the inserted ring reduces the fouling effect due to the flow separation occurring at the ring. Consequently, a venturi flowmeter with ring shows smaller pressure loss differences than the original configuration with no ring on fouling state. This research suggests an efficient and economic method of inserting a ring to reduce the pressure loss effects due to fouling.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39C no.11
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• pp.1023-1030
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• 2014

Beam steering algorithms using array antenna are mainly used in such a manner as anti-jamming method. However, the performance is changed according to the position of signals despite the same number of signals and the same received power. In this paper, we analyzed the effect of the position relationship of the signals on the performance of the beam steering algorithms. Therefore, we defined 'signal separation' as the minimum angle of the interference signals and the desired signal, and analysed the relationship between the C/N0 performance of beam steering algorithms as LCMV/PM and the signal separation. For simulation, we set many GPS signals and jamming signals compared to the degrees of freedom. And changed the position and the height of the receiver in order to obtain various signal separation angles. In addition, we examined the effects of signal separation and JSR on the anti-jamming performance by applying the loss factors of the received power. Through the research, there is a tendency that the performance of beam steering algorithms is increased with the increase of the signal separation, and signal separation is more severely affecting on the anti-jamming performance compared to the number of signals and JSR.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.37 no.3
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• pp.43-48
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• 2000

In this paper, vortical integrated transformers using bondwires are proposed and characterized for MMIC's (Monolithic Microwave Integrated Circuits) In a wide range of frequencies (1∼20 GHz), where full-wave analysis by the FEM (Finite Element Method) was adopted. The electrical characteristics of the proposed transformers are compared with those of the spiral transformer. We extracted mutual inductances from S -parameters. The vertical transformers using bondwires have not only low insertion loss but also reduce parasitic capacitances and dielectric loss due to their separation from substrates. It can be fabricated easily by used of the modern automatic wirebonding technology. It is expected that the proposed transformers are to improve the performance of MMIC's applied to impedance matching, and phase shifting circuits.

• Transactions of Materials Processing
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• v.24 no.1
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• pp.37-43
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• 2015

The purpose of the current study is to improve the clinching joint strength of aluminum and fiber metal laminates (FMLs) comprised of three layers. The joining of FML and Al 5052 by a conventional clinching joint has some disadvantages such as necking of the upper sheet, lack of interlocking, defects caused by the vertical load, and especially loss of strength of the composite material due to the low ductility. In the current study, a tapered-hole clinching method is proposed as an alternative for the joining of Al 5052 and FMLs. A hole with a tapered shape is formed before the joining process. The design parameters were evaluated using the Taguchi method for the geometry of the tapered hole in order to determine the maximum separation load. The diameter of the punch corner, clearance, punch stroke and the tapered length were used as the main variables in the Taguchi method. In conclusion, the contribution ratio for each of the fours variable examined was 35.07%, 22.44%, 21.32% and 14.11%, respectively. In addition, the appropriate combination of the design parameters can make a 5% improvement in the vertical direction joint strength.

• Proceedings of the Korean Geotechical Society Conference
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• 2003.03a
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• pp.597-604
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• 2003

In the soil washing process, the contaminants are usually removed by abrasion from soil particles using mechanical energy and water However, organic contaminants with low water solubility like polycyclic aromatic hydrocarbons (PAH) are remained on soil particles. Previous studies have shown that surfactant possessing amphipathic activity enhances the solubility of organic materials. For this reason solutions with surfactants have been used to improve removal of organic contaminants on soil washing process. But, in this manner, many problems were found like complete loss of surfactants and additional contamination by surfactant. The remediation method using microemulsion has been introduced to overcome these disadvantages. In this case, surfactants are recycled by phase separation of microemulsion after remediation. In microemulsion process, the surfactant will be recycled by phase separation of the microemulsion into a surfactant-rich aqueous phase and an oil phase after extraction. That is why remediation concept applying microemulsion as washing media has been Introduced. Suitable microemulsion have to be used in order to have the chance of refilling the soil after decontamination and to avoid any risk due to toxicity. The purpose of this research is to evaluate effect of microemulsion to remediation of contaminated soil. We performed test with various organic contaminants like Pyrene and BTEX, also compared efficiency of remediation in microemulsion process with soil washing

• Journal of the Korean GEO-environmental Society
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• v.15 no.11
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• pp.29-42
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• 2014

This paper investigates the effects of micro-tunneling on buried pipelines parametrically. A simplified numerical approach was developed and various parametric studies have been conducted to evaluate the effects of ground settlements on the response of buried pipelines. The controlled parameters included the pipe stiffness, ground loss magnitude, and pipe location with respect to a micro-tunnel. Maximum settlement and curvature along a pipeline have been investigated and compared among others for different conditions. In addition, the numerical results have been compared with a theoretical method by Attewell et al. (1986), which is based on a Winkler type linear-elastic solution. The comparison indicated that the response of buried pipes to micro-tunneling-induced ground settlements highly depends on the soil-pipe interaction including the separation and slippage of pipe from soil with the effects of the investigated parameters. Therefore, rather than using the theoretical method directly, it would be a better assessment of the response of buried pipelines to consider the soil-pipe interaction in more realistic conditions.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.67 no.6
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• pp.724-729
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• 2018

This paper presented an evaluation method for the efficiency uncertainty of an inverter-fed three-phase induction motor using FEM. The motor efficiency in the FEM is calculated by the IEC 60034-2-3 as in the actual test. In the process of evaluating the efficiency uncertainty, the difference between the finite element method and the actual test is the method of calculating the type-A / B standard uncertainty of the input quantity to estimate the efficiency and each losses. For the input quantities which can confirm the instantaneous values with respect to time, the type-A standard uncertainty in the FEM is calculated from the RMS values or average values having separate periods in the steady state. And, the type-B standard uncertainty in the finite element method is assumed to be zero. Also, this paper compared and analyzed the efficiency uncertainty evaluated by the proposed method and the efficiency uncertainty through the actual test.