• 대한교통학회지
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• 제13권3호
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• pp.67-86
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• 1995

It requires the detector system which can collect highly reliable traffic data in order to perform the real-time traffic signal control. This study is to decide the optimal shape of inductive loop for the real-time traffic signal control .This loop is located at the stopline in the signalized intersection for DS(Degree of Saturation) control. In order to find out the optimal shape of loop, 6types of experiments were performed . The results of the basic experiments of loops are as follows ; -the optimal number of turns for loop is 3 turns. -the impedance values of the loop detectors are similar to that of NEMA standards -the 1.8${\times}$4.5M loop is excellent for sensitivity in actual detection range of car length comparing to other shape of inductive loops. At the experimental of establishments of the optimal loop shape, it found that 1.8 4.5M loop has the highest values of $\DeltaL$ comparing to other types of loops, It means that the range of Lead-in cable length of this loop. And this loop is highly reliable in occpupancy time. Conclusivley, the 1.8${\times}$4.5M inductive loop is the optimal solution as a stop line loop detector for real -time traffic signal control.

• 전력전자학회논문지
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• 제27권1호
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• pp.80-83
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• 2022

This paper proposes an initial rotor polarity detection algorithm of a single-phase permanent magnet synchronous motor (SP-PMSM) related to stable open-loop starting for sensorless operation. Generally, the SP-PMSM needs an asymmetric air-gap structure to can avoid the initial starting failure at zero torque point. Therefore, the rotor polarity information can be obtained by using the DC offset current direction of a stator current through a high frequency voltage injection into an SP-PMSM with an asymmetric air gap. In this paper, the proposed rotor initial polarity detection algorithm is verified through several experimental results.

• The Plant Pathology Journal
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• 제31권3호
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• pp.219-225
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• 2015

The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over RT-PCR. RT-LAMP reactions were conducted with or without a set of loop primers since one out of six primers showed PVX specificity. Based on real-time monitoring, RT-LAMP detected PVX around 30 min, compared to 120 min for RT-PCR. By adding a fluorescent reagent during the reaction, the extra step of visualization by gel electrophoresis was not necessary. RT-LAMP was conducted using simple inexpensive instruments and a regular incubator to evaluate whether RNA could be amplified at a constant temperature instead of using an expensive thermal cycler. This study shows the potential of RT-LAMP for the diagnosis of viral diseases and PVX epidemiology because of its simplicity and rapidness compared to RT-PCR.

• 미생물학회지
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• 제47권2호
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• pp.103-109
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• 2011

Pectobacterium carotovorum subsp. carotovorum (PCC)는 세균성 무름병균으로 주로 감자, 양배추 등의 식물에서 질병을 일으킨다. 본 연구에서는 현장에서 신속하게 진단하기 위해 loop-mediated isothermal amplification법을 이용하여 1시간 내에 등온에서 검출 가능한 진단법을 개발하였으며, 이를 PCC-LAMP법이라 명명하였다. PCC의 lytic murein transglycolase 유전자를 특이적으로 증폭시키는 4개의 프라이머를 제작하였으며 최적 온도가 $61^{\circ}C$임을 확인하고 최적 조건을 확립하였다. 최적 조건을 바탕으로 4개의 프라이머가 $1{\times}10^3$ copies까지 검출하는 민감성을 확인할 수 있었다. 본 연구에서 개발된 PCC-LAMP법은 특이성 검사를 통해 PCC만이 특이적으로 검출됨을 확인하였으며, 이는 실제 시료에서도 적용 가능함을 확인하였다. PCC-LAMP법을 통하여 PCC를 신속하고 정확하게 검출함으로써 현장에서 유용하게 적용될 수 있을 것으로 사료된다.

• 비파괴검사학회지
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• 제35권5호
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• pp.321-331
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• 2015

냉각 위상잠금 적외선열화상 기법을 이용하여 원전 배관 루프시스템의 가열결함 검출의 선행연구를 통하여 냉각결함 검출조건의 적용에 관한 연구를 수행하였다. 배관의 결함가공은 감육 길이, 감육 깊이를 변화시켜 결함조건을 가공하여 루프시스템을 제작하였다. 사용된 장비는 적외선열화상 카메라와 냉각장치를 사용하였으며 냉각장치와 대상 루프시스템과의 거리는 2m로 고정시켜 실험을 수행하였다. 실험 결과의 분석을 위하여 냉각온도 분포, 위상데이터를 확보하고, 이를 분석하여 결함 길이를 측정하였다. 냉각결함 검출조건은 적외선열화상 데이터보다 위상잠금 적외선열화상 데이터가 측정 결과의 신뢰도가 높았다.

• 식물병연구
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• 제27권1호
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• pp.38-44
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• 2021

Xylella fastidiosa is the most damaging pathogen in many parts of the world. To increase diagnostic capability of X. fastidiosa in the field, the loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assay were developed to mqsA gene of citrate-synthase (XF 1535) X. fastidiosa and evaluated for specificity and sensitivity. Both assays were more robust than current published tests for detection of X. fastidiosa when screened against 16 isolates representing the four major subgroups of the bacterium from a range of host species. No cross reaction with DNA from healthy hosts or other species of bacteria has been observed. The LAMP and PCR assays could detect 10-4 pmol and 100 copies of the gene, respectively. Hydroxynaphthol blue was evaluated as an endpoint detection method for LAMP. There was a significant color shift that signaled the existence of the bacterium when at least 100 copies of the target template were present.

• The Plant Pathology Journal
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• 제34권6호
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• pp.499-505
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• 2018

Huanglongbing (HLB, Citrus greening disease) is one of the most devastating diseases that threaten citrus production worldwide. Although HLB presents systemically, low titer and uneven distribution of these bacteria within infected plants can make reliable detection difficult. It was known loop-mediated isothermal amplification (LAMP) method has the advantages of being highly specific, rapid, efficient, and laborsaving for detection of plant pathogens. We developed a new LAMP method targeting gene contained tandem repeat for more rapid and sensitive detection of Candidatus Liberibacter asiaticus (CLas), putative causal agent of the citrus huanglongbing. This new LAMP method was 10 folds more sensitive than conventional PCR in detecting the HLB pathogen and similar to that of real-time PCR in visual detection assay by adding SYBR Green I to mixture and 1% agarose gel electrophoresis. Positive reactions were achieved in reaction temperature 57, 60 and $62^{\circ}C$ but not $65^{\circ}C$. Although this LAMP method was not more sensitive than real-time PCR, it does not require a thermocycler for amplification or agarose gel electrophoresis for resolution. Thus, we expect that this LAMP method shows strong promise as a reliable, rapid, and cost-effective method of detecting the CLas in citrus and can be applied for rapid diagnosis is needed.

• 한국통신학회논문지
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• 제33권4A호
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• pp.440-449
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• 2008

본 논문에서는 Inmarsat M4 시스템의 수신기의 실제 구현에 적합한 16-QAM (Quadrature Amplitude Modulation) carrier recovery loop를 제안한다. Inmarsat M4 시스템 규격에서 권고하는 frequency tolerance는 ${\pm}924\;Hz$ (Signal bandwidth: 33.6 kHz) 로서 이러한 상대적으로 큰 주파수 옵셋 환경에서 안정된 동작이 가능한 carrier recovery loop 설계가 요구된다. 일반적인 PLL(Phase Locked Loop) 만을 이용한 carrier recovery loop는 상대적으로 큰 주파수 옵셋 환경에서 안정적인 성능을 보장할 수 없으며, 이에 따라 본 논문에서는 상대적인 주파수 옵셋이 큰 환경에서도 안정적이 동작이 가능한 Inmarsat M4 시스템을 위한 carrier recovery loop 루프를 제안한다. 제안된 carrier recovery loop는 우선 carrier recovery 이전에 UW 신호 detection 을 위해 주파수 옵셋에 강인한 differential filter 기반의 noncoherent 방식의 detector를 이용하여 UW detection을 수행하였으며, 이후 초기 주파수 옵셋 포착을 위해 UW(Unique Word) 신호를 이용한 차동 방식의 CP(Cross Product)-AFC를 적용하였다. 또한 일반적으로 알려진 16-QAM NDA (Non Data Aided) 방식 대신 안정적인 jitter 성능을 위하여 16-QAM DD(Decision Directed) 방식의 PLL 을 적용하여 위상 추적을 수행하였으며, 성능 검증을 통해 제안된 16-QAM carrier recovery loop가 만족스러운 성능과 신뢰성 있는 동작이 가능함을 입증하였다.

• Journal of Microbiology and Biotechnology
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• 제25권3호
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• pp.321-327
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• 2015

Nocardia salmonicida is one of the main pathogens of fish nocardiosis. The purpose of this study was to build a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of N. salmonicida. A set of four primers were designed from the 16S-23S rRNA intergenic spacer region of N. salmonicida, and conditions for LAMP were optimized as incubating all the reagents for 60 min at 64℃. LAMP products were judged with agar gel electrophoresis as well as with the naked eye after the addition of SYBR Green I. Results showed the sensitivity of the LAMP assay was 1.68 × 10³ CFU/ml (16.8 CFU per reaction) and 10-fold higher than that of PCR. The LAMP method was also effectively applied to detect N. salmonicida in diseased fish samples, and it may potentially facilitate the surveillance and early diagnosis of fish nocardiosis.

• Journal of Power Electronics
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• 제13권5호
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• pp.884-895
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• 2013

In this paper, the Fourier-based PLL (Phase-locked Loop) is introduced with a new structure, capable of selective harmonic detection in single and three-phase systems. The application of the FB-PLL to harmonic detection is discussed and a new model applicable to three-phase systems is introduced. An analysis of the convergence of the FB-PLL based on a linear model is presented. Simulation and experimental results are included for performance analysis and to support the theoretical development. The decomposition of an input signal in its harmonic components using the Fourier theory is based on previous knowledge of the signal fundamental frequency, which cannot be easily implemented with input signals with varying frequencies or subjected to phase-angle jumps. In this scenario, the main contribution of this paper is the association of a phase-locked loop system, with a harmonic decomposition and reconstruction method, based on the well-established Fourier theory, to allow for the tracking of the fundamental component and desired harmonics from distorted input signals with a varying frequency, amplitude and phase-angle. The application of the proposed technique in three-phase systems is supported by results obtained under unbalanced and voltage sag conditions.