• Title/Summary/Keyword: Long-term Preservation

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Revolution of Dead-Cell: Production of New Generation by Intracytoplasmic Dried-Sperm Injection in Mammal

  • Kim, Duk-Im;Kim, Chang Jin;Lee, Kyung-Bon
    • Reproductive and Developmental Biology
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    • v.39 no.3
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    • pp.69-76
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    • 2015
  • In a conventional sense, dried-spermatozoa are all dead and motionless due to the lost of their natural ability to penetrate oocytes both in vivo and in vitro. However, their nuclei are completely able to contribute to normal embryonic development even after long-term preservation in a dried state when the dried-spermatozoa are microinjected into the oocytes. In this sense, dried spermatozoa must still be alive. Thus, defining spermatozoa as alive or dead seems rather arbitrary. Several drying method of sperm including freeze-drying, evaporative/convective-drying and heat-drying were represented in this review. Although the drying protocol reported here will need further improvement, the results suggest that it may be possible to store the male genetic resources.

Glucose metabolism and evaluation of hypoglycemia in neonates (신생아에서의 포도당 대사와 저혈당증의 평가)

  • Kim, Eun Young
    • Clinical and Experimental Pediatrics
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    • v.50 no.3
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    • pp.223-229
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    • 2007
  • The fetus is completely dependent on mother for glucose and other nutrient transfer across the placenta. At birth, when the maternal supply is discontinued, the neonate must adjust to an independent existence. The changes in the neonate's glucose homeostasis during this transition to the extrauterine environment are influenced by the mother's metabolism and intrinsic fetal and placental problems. Maturation of carbohydrate homeostasis results from a balance between substrate availability and coordination of developing hormonal, enzymatic, and neural systems. These mechanisms may not be fully developed in neonates, so the neonate is vulnerable to carbohydrate disequilibrium resulting in damage to the central nervous system. Hypoglycemia is a relatively common metabolic problem seen during newborn care. However its definition, management and long term sequalae remain controversial. Hyporglycemia occurs frequently as a transient disorder with excellent prognosis. It also may persist and recur and cause permanent neurological complications. Although the key to effective treatment of hypoglycemia is diagnostic specific, the maintenance of euglycemia is critical to the preservation of central nervous system function. This article discusses physiology of perinatal glucose homeostasis, focusing on evaluation and treatment of hypoglycemia.

Understanding Ancient Human Subsistence through the Application of Organic Residue Analysis on Prehistoric Pottery Vessels from the Korean Peninsula

  • Kwak, Seungki;Kim, Gyeongtaek
    • Journal of Conservation Science
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    • v.36 no.4
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    • pp.244-254
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    • 2020
  • This study investigates prehistoric human subsistence and pottery use on the Korean peninsula through the organic residue analysis of archaeological potsherds. Ancient human subsistence is one of the core topics in Korean archaeology. However, due to the high acidity of sediments, which prevents the long-term preservation of organic remains, archaeologists have been short of critical information on how these early prehistoric dwellers lived. Ceramic vessels can contain well-preserved lipids originating from past culinary practices. For a better understanding of human subsistence on the prehistoric Korean peninsula, food-processing behaviors were reconstructed by analyzing ancient lipids extracted from a pottery matrix. The potsherd samples used in the analysis in this paper were collected from major prehistoric habitation sites. The results show that subsistence strategies differed according to both location and time period and reveal how organic residue analysis can contribute to a better understanding of prehistoric human subsistence strategies.

Groundwater Flow model of Drawdown and Recovery Due to Watertight Tunnel Excavation and Design Example for Lining (터널시공에 따른 지하수위 변화의 모델링과 이를 고려한 완전방수 터널의 라이닝 설계 예)

  • 남기천;이형원;배정식;나경웅
    • Tunnel and Underground Space
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    • v.4 no.1
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    • pp.24-30
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    • 1994
  • Although a dry-system tunnel is not good for reasons fo economy and construction, it has been applied to some tunnels under construction owing to the advantages of good long-term maintenance of tunnel, prevention of consolidation settlement due to the drawdown of groundwater, preservation of the ecosystem, cutailment of operation cost, and so on. The stability of groundwater and the change of the applied water pressure after water proofing were analysed by the finite element method. Using this result, an example of designing the secondary lining for the dry-system tunnel which is to be constructed in low-permeability hard rock was presented.

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A Design of Metadata for Government Electronic Records Long-Term Preservation (국가 전자기록물 영구보존을 위한 메타데이터 설계)

  • Hwang Yun-Young;Lim Hyusk-Soo;Lee Kyu-Chul
    • Proceedings of the Korean Information Science Society Conference
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    • 2005.07b
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    • pp.88-90
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    • 2005
  • 전자정부 사업이 추진되면서 정부의 각급기관은 전자적으로 기록물을 생성 및 유통하고 있다. 각급기관에서 생성된 전자기록물은 관리 및 열람${\cdot}$활용을 위해 자료관시스템으로 이관되며, 준영구 이상의 전자기록물은 영구보존을 위해 국가기록원으로 이관되어 보존된다. 전자기록물을 효과적으로 영구보존 및 활용하기 위해서는 여러 종류의 메타데이터가 필요한데, 이를 위해 외국에서는 전자기록물 메타데이터를 표준화하여 사용하고 있다. 본 논문에서는 전자기록물 영구보존 메타데이터 요소를 정의한 ISO 15489와 호주 빅토리아주의 메타데이터 및 우리의 메타데이터를 분석하여 우리의 현실에 맞는 메타데이터를 제안하고자 한다.

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A Study on the Status and Component of Digital Archive (디지털 아카이브의 현황 및 구성 요소에 관한 연구)

  • 최원태
    • Journal of the Korean Society for Library and Information Science
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    • v.35 no.2
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    • pp.23-40
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    • 2001
  • Digital technologies we used increasingly for information production. storage, and distribution. This study provides an overview of the background and related issues for preserving digital informations in the long-term including technical, economic and national concerns for the digital archive. Selection, identification, preservation, and maintenance components of the digital archive is be investigated.

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Nonoperative Treatment of Achilles Tendinopathy (아킬레스 건병증의 비수술적 치료)

  • Jeong, Jae Jung
    • Journal of Korean Foot and Ankle Society
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    • v.25 no.2
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    • pp.66-71
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    • 2021
  • Achilles tendinopathy has seen good results with conservative management. However, the management of Achilles tendinopathy lacks evidence-based support, and tendinopathy patients are at risk of long-term morbidity with unpredictable clinical outcomes. Data suggests that 29% of tendinopathy patients required surgical intervention during the follow-up period. Chronic pain after damage to the Achilles tendon is a result of incomplete recovery of fibrous tissue. Recently, many procedures, including various injection treatments, have been tried without understanding proper preservation techniques and procedures for faster tendon recovery, especially for patients who want to quickly return to their daily lives. This article is an extensive literature review on nonoperative management of Achilles tendinopathy.

Analysis of the Change in Density of Development And Environmental Restrictions Conflict Prediction in Pyeongchang (개발 밀도의 변화 분석과 환경규제 갈등 예측 -평창을 사례로-)

  • Bae, Sun-Hak
    • Journal of the Korean association of regional geographers
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    • v.15 no.2
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    • pp.282-291
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    • 2009
  • This research predicts the spatial discord which relates with a restriction using 1915 and 2005 spatial data. In this research, difference of relative spatial density is measured and predicted the discord which relates with an environmental restriction in Pyeongchang. First, research area Pyeonchang's relative crowding degree of the building was strengthened from 1915 to 2005. When classifies a change type, formed the strong hold with new regulation and grew types and at the strong hold where contiguous concentration is progress types, general the influence weakening types and the change almost nil types. The next is the result which analyzes the long and short terms discord for the environmental restriction which is forecast from the research area. That is forecast with the fact that the discord between of development and preservation will be big with long and short terms in Jinbu-Myeon, Pyeongchang-Eup city center angles and 31 national road circumferences. And in Daegwanryeong-Myeon the discord is big short-term but with the fact that the discord will be weakened long-term. Bangrim-Myeon, now the discord is weak but the discord will be strengthened long-term. This result could be applied with fundamental data for weakening the spatial discord of the area.

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Enhanced Production of Phaeodactylum tricornutum (Marine Diatoms) Cultured on a New Medium with Swine Wastewater Fermented by Soil Bacteria

  • Kim, Mi-Kyung;Chang, Moo-Ung
    • Journal of Microbiology and Biotechnology
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    • v.16 no.12
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    • pp.1947-1953
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    • 2006
  • There have been a number of studies of methods for recycling animal wastewater to provide new bioresources. In the present work, a marine algal culture medium, designated KEP II, was prepared by adding swine waste (3% v/v) fermented by soil bacteria to a dilution of f/2 culture medium (CT). When Phaeodactylum tricornutum was grown in batch culture in KEP II, the cells lasted long at the exponential phase producing the specific growth rate and biomass; the production of total amino acids and secondary metabolites rose up to 5-fold. It also substantially enhanced the maximum quantum yield of photo system (PS) II of P. tricornutum, greatly increased the level of thylakoid membranes containing PS, and stimulated the production of pyrenoids, including enzymes for $CO_2$ fixation in chloroplasts. KEP II should improve the cost efficiency of industrial mass batch cultures and the value of microalgae for long-term preservation of fresh aquaculture feed as well as production of anticancer and antioxidant agents. Specifically, a low-cost medium for growing the diatoms of aquaculture feed will be economically advantageous.

Optimization Conditions for Cryopreservation of Deutzia paniculata Nakai, Endangered Plant

  • Seol, Yuwon;Yong, Seong Hyeon;Choi, Eunji;Jeong, Mi Jin;Suh, Gang Uk;Lee, Cheul Ho;Choi, Myung Suk
    • Journal of Forest and Environmental Science
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    • v.36 no.4
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    • pp.274-280
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    • 2020
  • As the importance of biological resources increases, the conservation technology is becoming important for rarities. This study was conducted to establish an efficient cryopreservation conditions for the Deutzia paniculata, endangered plant species, by using both cryopreservation methods of vitrification and encapsulation. As a result, the sucrose pretreatment seed viability showed up to 30.7% in the treatments. The cryoprotectant treatment improved the viability of the seeds, and was found to be excellent in the vitrification method using PVS3. The vitrification method had over 10% higher germination rate than the seeds preserved by encapsulation. In addition, the germination rate showed a significant difference according to the cryopreservation treatment time, and the germination rate of seeds decreased very much as the long time became longer. Plants germinated from preserved seed in liquid nitrogen showed poor growth compared to untreated, and good growth in PVS3 120 minutes. In addition, the growth of germinated plants by liquid nitrogen treatment time was better in the vitrification method. These results are expected to be useful for long-term preservation of D. paniculata, endangered plants.