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http://dx.doi.org/10.7747/JFES.2020.36.4.274

Optimization Conditions for Cryopreservation of Deutzia paniculata Nakai, Endangered Plant  

Seol, Yuwon (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Yong, Seong Hyeon (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Choi, Eunji (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Jeong, Mi Jin (Division of Plant Resources, Korea National Arboretum)
Suh, Gang Uk (Division of Plant Resources, Korea National Arboretum)
Lee, Cheul Ho (DMZ Botanic Garden, Korea National Arboretum)
Choi, Myung Suk (Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
Publication Information
Journal of Forest and Environmental Science / v.36, no.4, 2020 , pp. 274-280 More about this Journal
Abstract
As the importance of biological resources increases, the conservation technology is becoming important for rarities. This study was conducted to establish an efficient cryopreservation conditions for the Deutzia paniculata, endangered plant species, by using both cryopreservation methods of vitrification and encapsulation. As a result, the sucrose pretreatment seed viability showed up to 30.7% in the treatments. The cryoprotectant treatment improved the viability of the seeds, and was found to be excellent in the vitrification method using PVS3. The vitrification method had over 10% higher germination rate than the seeds preserved by encapsulation. In addition, the germination rate showed a significant difference according to the cryopreservation treatment time, and the germination rate of seeds decreased very much as the long time became longer. Plants germinated from preserved seed in liquid nitrogen showed poor growth compared to untreated, and good growth in PVS3 120 minutes. In addition, the growth of germinated plants by liquid nitrogen treatment time was better in the vitrification method. These results are expected to be useful for long-term preservation of D. paniculata, endangered plants.
Keywords
cryopreservation; endangered plants; vitrification; encapsulation;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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1 Murashige T, Skoog F. 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant 15: 473-497.   DOI
2 Nishizawa S, Sakai A, Amano Y, Matsuzawa T. 1993. Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic suspension cells and subsequent plant regeneration by vitrification. Plant Sci 91: 67-73.   DOI
3 Normah MN, Sulong N, Reed BM. 2019. Cryopreservation of shoot tips of recalcitrant and tropical species: advances and strategies. Cryobiology 87: 1-14.   DOI
4 Paek KY, Ko JA, Gwak SS, Kim JY, Kim CG, Kim HH, Park KI, Park SY, Park SC, Liu JR, Yoo CY, Yun JG, Lee GJ, Jeon SI, Jung M, Jeong BR, Han BH, Han JH, Hyung NI. 2016. Plant Biotechnology. Hyangmunsa, Seoul, 363 pp.
5 Park JI, Chung WS. 2009. Cryopreservation of Catalpa ovata G.Don seeds. J Agr Sci 25: 30-34.
6 Park JS. 2016. Growth Environmental Characteristics of Paniculate Korean Deutzia Populations in Korea. MS thesis. Yeungnam University, Gyeongsan, Korea. (in Korean)
7 Paulet F, Engelmann F, Glaszmann JC. 1993. Cryopreservation of apices of in vitro plantlets of sugarcane (Saccharum sp. hybrids) using encapsulation/dehydration. Plant Cell Rep 12: 525-529.   DOI
8 Peter MV. 1994. Beyond Global Warming: Ecology and Global Change. Ecology 75: 1861-76.   DOI
9 Sakai A, Kobayashi S, Oiyama I. 1990. Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9: 30-33.   DOI
10 Seo MJ. 2005. Cryopreservation of Herbaceous Peony (Paeonia lactiflora Pall.) by Encapsulation-dehydration of Dormant Shoot Tips. MS thesis. Kyungpook National University, Daegu, Korea. (in Korean)
11 Ahn YH. 2001. Cryopreservation and Germination of Native Aquilegia Species Seeds by Predehydration Treatment. Korean J Plant Res 14: 251-258.
12 Yang WH, Yong SH, Park DJ, Seol YW, Choi EJ, Jeong MJ, Suh GU, Lee CH, Choi MS. 2018. Optimization Conditions for Cryopreservation of Potentilla Discolor Bunge. J Korean Soc For Sci 107: 258-265.
13 Yong SH, Park DJ, Yang WH, Seol YW, Choi EJ, Jeong MJ, Suh GU, Lee CH, Choi MS. 2019. Cryopreservation of Sievers Wormwood (Artemisia sieversiana Ehrh. Ex Willd.) Seeds by Vitrification and Encapsulation. For Sci Technol 15: 180-186.
14 Cottrell HJ. 1947. Tetrazolium salt as a seed germination indicator. Nature 159: 748.   DOI
15 Ahn YH. 2002. Cryopreservation of winter vegetation buds of Betula platyphylla var. japonica in liquid nitrogen. Korean J Plant Res 15: 89-95.
16 Association of Official Seed Analysts. Seed Vigor Test Committee. 1983. Seed Vigor Testing Handbook: Contribution no. 32 to the Handbook on Seed Testing. Association of Official Seed Analysts, Boise, 88 pp.
17 Bajaj YPS. 1976. Regeneration of Plants from Cell Suspensions Frozen at -20, -70 and -196℃. Physiol Plant 37: 263-268.   DOI
18 Chang CS, Kim H, Kim YS. 2001. Reconsideration of rare and endangered plant species in Korea based on the IUCN Red List Categories. Korean J Plant Tax 31: 107-142.   DOI
19 Choi YC, Ryu KS, Bang HS. 2000. Cryopreservation of Mulberry(Morus) Seeds in Liquid Nitrogen(LN2). Korean J Seric Sci 42: 1-5.
20 Davis AJ, Jenkinson LS, Lawton JH, Shorrocks B, Wood S. 1998. Making mistakes when predicting shifts in species range in response to global warming. Nature 391: 783-786.   DOI
21 Engelmann F. 1991. In vitro conservation of tropical plant germplasm- a review. Euphytica 57: 227-243.   DOI
22 Engelmann F. 2004. Plant cryopreservation: progress and prospects. In Vitro Cell Dev Biol 40: 427-433.   DOI
23 Gonzalez-Arnao MT, Engelmann F. 2006. Cryopreservation of plant germplasm using the encapsulation-dehydration technique: review and case study on sugarcane. Cryo Letters 27: 155-168.
24 Han SH, Kim CS, Jang SS, Lee HJ. 2004. Effects of Cryopreservation on the Seed Germination and Growth Properties of Seedlings of Maackia amurensis. Korean J Plant Res 17: 75-81.
25 Jeon SM, Kim CK. 2014. Cryopreservation of Chrysanthemum morifolium cv. 'White ND' Shoot Tips using Encapsulation-Dehydration-Vitrification Method. Curr Res Agric Life Sci 32: 99-103.   DOI
26 Kim SW, In DS, Jeong WJ, Woo JW, Jung M, Liu JR. 1998. Plant Regeneration from Cryopreserved Embryogenic Cell Suspension Cultures of Cucumber. Korean J Plant Tissue Cult 25: 501-505.
27 Kartha KK, Engelmann F. 1994. Cryopreservation and Germplasm Storage. In: Plant Cell and Tissue Culture (Vasil IK, Thorpe TA, eds). Kluwer Academic, Dordrecht, pp 195-230.
28 Kim HH, Yi JY, No NY, Cho GT, Yoon MS, Baek HJ, Kim CK. 2010. Development of cryopreservation protocols through droplet-vitrification and its application to vegetatively propagated crop germplasm. Proceedings of Korean J of Plant Res 14: 12-12.
29 Kim KY. 2010. Cryopreservation of Cultivated Lily Bulblets by Droplet-Vitrification Procedure. MS thesis. Kyungpook National University, Daegu, Korea. (in English)
30 Kim SW, Jeong WJ, Min SR, Bae KS, Liu JR. 1995. Plant regeneration from cryopreserved embryogenic cell suspension cultured of Korean rice (Oriza sativa L.) cultures. Korean J Plant Tissue Cult 22: 115-119.
31 Kim YW, Kim JC, Yun Y, Noh ER, Son SH. 1999. Cryopreservation of Embryogenic Tissue and Plant Regeneration in Larix leptolepis. Korean J Plant Tissue Cult 26: 229-233.
32 Lee JY. 2011. Permanent preservation of genetic resources through cryopreservation technology. The Science & Technology 501: 87-89.
33 Lee YK, Park SU, Kim HH. 2011. Cryopreservation of chrysanthemum shoot tips using the droplet-vitrification technique. Korean J Agric Sci 38: 227-233.   DOI
34 Lee YY, Lee MH, Yi JY, Lee TY, Son EH, Park HJ. 2017. Response of germination rate and ascorbate peroxidase activity to cryopreservation of Perilla (Perilla frutescens) seeds with variable initial viabilities. Korean J Environ Agric 36: 256-262.   DOI