• Genomics & Informatics
• /
• 제21권2호
• /
• pp.19.1-19.13
• /
• 2023

Liver cancer, particularly hepatocellular carcinoma (HCC), poses a significant global threat to human lives. To advance the development of innovative diagnostic and treatment approaches, it is essential to examine the hidden features of HCC, particularly its 3D genome architecture, which is not well understood. In this study, we investigated the 3D genome organization of four HCC cell lines-Hep3B, Huh1, Huh7, and SNU449-using in situ Hi-C and assay for transposase-accessible chromatin sequencing. Our findings revealed that HCC cell lines had more long-range interactions, both intra-and interchromosomal, compared to human mammary epithelial cells (HMECs). Unexpectedly, HCC cell lines displayed cell line-specific compartmental modifications at the megabase (Mb) scale, which could potentially be leveraged in determining HCC subtypes. At the sub-Mb scale, we observed decreases in intra-TAD (topologically associated domain) interactions and chromatin loops in HCC cell lines compared to HMECs. Lastly, we discovered a correlation between gene expression and the 3D chromatin architecture of SLC8A1, which encodes a sodium-calcium antiporter whose modulation is known to induce apoptosis by comparison between HCC cell lines and HMECs. Our findings suggest that HCC cell lines have a distinct 3D genome organization that is different from those of normal and other cancer cells based on the analysis of compartments, TADs, and chromatin loops. Overall, we take this as evidence that genome organization plays a crucial role in cancer phenotype determination. Further exploration of epigenetics in HCC will help us to better understand specific gene regulation mechanisms and uncover novel targets for cancer treatment.

• 한국수자원학회논문집
• /
• 제55권10호
• /
• pp.723-736
• /
• 2022

본 연구에서는 금강권역을 대상으로 최대 12개월까지 선행예측이 가능한 월 강수량 예측모형을 구축하였으며, 예측모형 구축에는 다중회귀분석과 인공신경망의 두 가지 통계적 기법을 적용하였다. 예측인자 후보로 NOAA에서 제공하는 글로벌 기후패턴 39종과 금강권역에 대한 기상인자 8종 등 총 47종의 기후지수를 활용하였다. 예측대상월을 기준으로 과거 40년간의 월 강수량과 기후지수와의 지연상관성 분석을 통해 상관도가 높은 기후지수를 예측인자로 활용하여 다중회귀모형 및 인공신경망 모형을 구축하였다. 1991~2021년에 대해 매월 예측결과의 평균값과 관측값과의 적합도를 분석한 결과, 다중회귀모형은 PBIAS -3.3~-0.1%, NSE 0.45~0.50, r 0.69~0.70으로 분석되었으며, 인공신경망모형은 PBIAS -5.0~+0.5%, NSE 0.35~0.47, r 0.64~0.70로, 다중회귀모형에 의해 도출된 예측치의 평균값이 인공신경망모형보다 관측치에 좀 더 근접한 것으로 나타났다. 각 월의 예측범위 안에 관측치가 포함될 확률을 분석한 결과에서는 다중회귀모형이 57.5~83.6%(평균 72.9%), 인공신경망모형의 경우에는 71.5~88.7%(평균 81.1%)로 인공신경망모형 결과가 우수한 것으로 나타났다. 3분위 예측확률을 비교한 결과는 다중회귀모형의 경우에는 25.9~41.9%(평균 34.6%), 인공신경망모형은 30.3~39.1%(평균 34.7%)로 비슷하며, 두 모형 모두 평균 33.3% 이상으로 월 강수량에 대한 장기예측성을 확인 할 수 있었다. 이상과 같이 두 모형의 예측성 차이는 비교적 크지 않은 것으로 나타났으나, 예측범위에 대한 적중률이나 3분위 예측확률로부터 판단할 때 예측성에 대한 월별 편차는 인공신경망모형의 결과가 상대적으로 작게 나타났다.

• 대한전자공학회논문지SP
• /
• 제49권4호
• /
• pp.77-84
• /
• 2012

얼굴인식 기술은 지능형 보안, 웹에서 콘텐츠 검색, 지능로봇의 시각부분, 머신인터페이스 등, 활용이 광범위 하다. 그러나 일반적으로 대상자의 표정과 포즈 변화, 주변의 조명 환경과 같은 문제가 있으며 이와 더불어 원거리에서 획득한 영상의 경우 저해상도를 비롯하여 블러와 잡음에 의한 영상의 열화 등의 여러 가지 어려움이 발생한다. 본 논문에서는 포톤 카운팅(Photon-counting) 선형판별법(Linear Discriminant Analysis)을 이용한 다중 분류기(Classifier)에 의한 판정을 융합하여 얼굴 영상 인식을 수행한다. Fisher 선형판별법은 집단 간 분산을 최대로 하고 집단 내 분산을 최소로 하는 공간으로 선형 투영하는 방법으로, 학습영상의 수가 적을 경우 특이행렬 문제가 발생하지만 포톤카운팅 선형 판별법은 이러한 문제가 없으므로 차원축소를 위한 전 처리 과정이 필요 없다. 본 논문의 다중 분류기는 포톤 카운팅 선형판별법의 유클리드 거리(Euclidean Distance) 또는 정규화된 상관(Normalized Correlation)을 적용하는 판정규칙에 따라 구성된다. 다중분류기의 판정의 융합은 각 분류기 cost의 정규화(Normalization), 유효화(Validation), 그리고 융합규칙(Fusion Rule)으로 구성된다. 각 분류기에서 도출된 cost는 같은 범위로 정규화된 후 유효화 과정에서 선별되고 Minimum, 또는 Average, 또는 Majority-voting의 융합규칙에 의하여 융합된다. 실험에서는 원거리에서 획득한 효과를 구현하기 위하여 고해상도 데이터베이스 영상을 인위적으로 Unfocusing과 Motion 블러를 이용하여 열화하여 테스트하였다. 실험 결과는 다중분류기 융합결과의 인식률은 단일분류기보다 높다는 것을 보여준다.

• 대한불안의학회지
• /
• 제14권1호
• /
• pp.44-52
• /
• 2018

Objective : The present study had examined the psychiatric symptoms and physical health consequences for the bereaved parents of the high school students who died in the 2014 Sewol ferry accident. Methods : Forty bereaved parents participated in the study. The authors administered self-report questionnaires about the parents' health behaviors and psychiatric symptoms. The authors also conducted laboratory tests to assess the parents' physical health at 18 and 30 months after the accident. Univariate descriptive statistics were performed to report the prevalence and severity of psychiatric symptoms and health-related behaviors. Paired t-test and Mcnemar test were performed to compare the 18-and 30-month findings. Correlation analysis between psychiatric symptoms and laboratory findings were performed to find a relationship between the two variables. Results : At 30 months after the accident, most of the bereaved parents still appeared to suffer from complicated grief (97.5%), post traumatic stress disorder (80%), insomnia (77.5%) and severe depression (62.5%) based on the scores on the Inventory of Complicated Grief (ICG), the PTSD Check List-5 (PCL-5), the Insomnia Severity Index (ISI) and the Patient Health Questionnaire-9 (PHQ-9). One quarter of the bereaved parents reported high-risk drinking, and 47.5% reported increased drinking amount and frequency after the accident. In objective laboratory results, 55% of the bereaved parents were obese as defined by body mass index ${\geq}25$. The parents' mean low-density lipoprotein shows a significant increase over time (118.5 mg/dL at 18 months. vs. 132.5 mg/dL at 30 months. paired t-test t=-4.061, $$p{\leq_-}0.001$$). Total cholesterol and low-density lipoprotein at 30 months after the accident were in clinically borderline high range. In correlation analysis, triglyceride was positively correlated with ISI. Conclusion : The loss of children in the Sewol ferry accident, a disaster caused by human error, continued to have considerable impact on the victims' parents' mental and physical health 18 and 30 months after the accident. A longitudinal study following the parents' physical health would be necessary to investigate the long-term effects of this traumatic experience on physical health.

• 인간식물환경학회지
• /
• 제23권6호
• /
• pp.637-646
• /
• 2020

Background and objective: The main objective of this study is to measure the amount of particulate matter (PM) reduction under different characteristics of leaves in 18 different species of indoor plants. Methods: First, a particular amount of PM was added to the glass chambers (0.9×0.86×1.3 m) containing the indoor plant (height = 40 ± 20 cm), and the PM concentration were measured at 2-hour intervals. The experiment with the same conditions was conducted in the empty chamber as the control plot. Results: The range of PM reduction per unit leaf area of 18 species of experimental plants was 3.3-286.2 ㎍·m^-2 leaf, total leaf area was 1,123-4,270 cm², and leaf thickness was 0.14-0.80 mm and leaf size 2.27-234.47 cm². As time passed, the concentration of PM decreased more in the chamber with plants than in the empty chamber. Among the 18 indoor plants, the ones with the greatest reduction in PM2.5 in 2 hours and 4 hours of exposure to PM2.5 were Pachira aquatica and Dieffenbachia amoena. As the exposure time of PM increased, the efficiency of reducing PM2.5 was higher in plants with medium-sized leaves than plants with large or small leaves. The effect of reducing PM2.5 was higher in linear leaves than round or lobed leaves. Plants with high total leaf area did not have advantage in reducing PM because the leaves were relatively small and there were many overlapping parts between leaves. In the correlation between leaf characteristics and PM 2.5 reductions, all leaf area and leaf thickness showed a negative and leaf size showed a positive correlation with PM reduction. Conclusion: The PM reduction effect of plants with medium-sized leaves and long linear leaves was relatively high. Moreover, plants with a large total leaf area without overlapping leaves will have advantaged in reducing PM. Plants are effective in reducing PM, and leaf characteristics are an important factor that affects PM reduction.

• 터널과지하공간
• /
• 제33권5호
• /
• pp.414-424
• /
• 2023

본 연구에서는 픽커터의 다양한 형상변수들과 그에 따른 절삭 가능 암반 조건들에 대한 총 326개의 정보를 수집하고 데이터베이스를 구축하였다. 이와 같이 구축한 데이터베이스를 대상으로 통계분석을 실시한 결과, 픽커터의 형상을 정의하는 변수들 사이에는 유의미한 양(+)의 상관관계가 존재하는 것으로 나타났다. 특히, 픽커터의 길이 관련 변수들과 텅스텐 카바이드 삽입재의 형상 변수들 사이의 상관관계가 높게 나타났다. 픽커터 샤프트의 직경도 삽입재의 형상변수들과 밀접한 상관관계를 나타내었다. 반면, 네 가지 조건으로 정의한 암반 조건에 대한 변수와 픽커터의 형상 변수들 사이에는 뚜렷한 상관관계를 확인하기 어려웠다. 이는 암반의 불확실성으로 인해 픽커터의 적용 대상을 수치가 아닌 적용 가능 범위로 제시했기 때문인 것으로 사료된다. 단, 암반이 강해질수록 픽커터 형상변수들의 평균값이 증가하는 경향을 나타내었다. 그러나 픽커터의 길이 관련 변수들은 암반이 강해질수록 오히려 작아지는 것으로 나타났는데, 이는 경암반에서 길이가 긴 픽커터를 사용할 때 발생할 수 있는 모멘트의 발생을 줄이기 위한 방안으로 사료된다.

• 한국수자원학회논문집
• /
• 제52권7호
• /
• pp.493-503
• /
• 2019

본 연구의 목적은 영상 처리를 통하여 자갈 이상의 조립질 재료로 이루어진 하상의 입도 분포를 추정하는 기법을 개발하는 것이다. 전체 과정은 개별 입자의 영상 분석과정과 혼합 입경으로 이루어진 하상 영상에서 입자를 추출하고 분석하여 입도 분포를 추정하는 두 과정으로 이루어졌다. 먼저 개별 입자들의 영상에 나타난 평면 특성(장축, 중간축, 면적 등)이 실제 입자와 어떤 관계를 가지는지 분석하였다. 이를 위하여, 3개 중소하천에서 240개의 자갈 시료를 채취한 뒤, 각 입자의 장축, 중간축, 단축의 길이와 중량을 측정하였다. 또, 채취된 입자를 하나씩 촬영하여 영상을 만들고, 영상에서 장축과 중간축, 투영면적을 계측하였다. 영상에서 계측된 정보와 버니어 캘리퍼스와 저울을 이용하여 실제 측정한 자료를 비교하였다. 입자의 개별 측정 결과 자갈 하천의 하상 재료의 형상계수는 0.514~0.585 이었다. 또한 자갈의 중량은 장축이나 중간축보다는 투영면적과의 상관성이 더 높다. 따라서 영상의 투영면적에서 중량을 산정할 수 있는 관계식을 작성하였다. 또, 자갈하상을 촬영한 영상에서 ImageJ 프로그램를 이용하여 입자 하나하나의 윤곽을 검출한 뒤, 입자의 투영면적을 산출하였다. 그리고 투영면적과중량의 관계식을 이용하여, 중량입도분포를 추정하는 방법을 제시하였다. 제안된 방법을 3개 하천에 적용해 본 결과 비교적 상당한 정확도로 자갈의 입도분포를 추정할 수 있었다. 다만, 추정된 입도곡선에서 일부분은 실측된 입도곡선과 차이를 보였으며, 이는 추후 개선되어야 할 부분이다.

• 한국광물학회:학술대회논문집
• /
• 한국광물학회.한국암석학회 2001년도 공동학술발표회 논문집
• /
• pp.75-75
• /
• 2001

We report Sr, Nd and Pb isotope data of clinopyroxene separates from ultramafic xenoliths and their host basaltic rocks in Jeju Island, Baekryeong Island, Boeun and Ganseong, Korea. The isotopic data of the xenoliths and host basalts are distinctly different from those of Korean basement rocks. Except for two xenoliths from Ganseong, all samples in this study have isotopic ratios within the combined range of MORB-OIB data. All basaltic rocks have Nd-Sr-Pb isotope compositions different from those of xenoliths, indicating that the host basaltic magma did not derive from the lithospheric mantle where the xenoliths originated. The range of isotopic composition of xenoliths is much greater than that observed in host basalts, which reflects small-scale heterogeneity of the lithospheric mantle. The greater isotopic heterogeneity of the lithospheric mantle probably reflects its long-term stability. The spinel peridotite xenolith data of Jeju Island, Baekryeong Island and Boeun display mixing hyperbolas between DMM and EM II end members. Since Jeju basalts have EM II-like isotopic signature, the mixing relationship shown by the isotopic data of the Jeju xenoliths can be interpreted as the result of infiltration of metasomatic fluid or melt derived from basaltic magma into DMM-like lithospheric mantle. In contrast to other xenolith sites, the Ganseong xenoliths are dominantly clinopyroxene megacryst and pyroxenite. Clinopyroxene megacrysts have different isotopic ratios from their host basalt, reflecting its exotic origin. Two Ganseong xenoliths (wherlite and clinopyroxenite) have much enriched Sr and Nd isotopic ratios and Nd model ages of 2.5-2.9 Ga, and plot in an array away from the MORB-OIB field. The mantle xenoliths from Korean Peninsula have similar $\^$87/Sr/$\^$86/Sr,$\^$143/Nd/$\^$144/Nd and $\^$207/Pb/$\^$204/Pb ratios to, but higher $\^$208/Pb/$\^$204/Pb ratios than, those from eastern China, indicating that Korean xenoliths are derived from the lithospheric mantle with higher Th/U ratio compared with Chinese ones. The isotopic data of xenolith-bearing basalts of Baekryeong Island and Ganseong, along with Ulreung and Dok Islands, show a mixing trend betlveen DMM and EM I in Sr-Nd-Pb isotopic correlation diagrams, which is also observed in tile northeastern Chinese basalts. However, the Jeju volcanic rocks show an EM II signature that is observed in southeastern Chinese basalts. The isotopic variations in volcanic rocks from the northern and southern portions of the East Asia reflect a large-scale isotopic heterogeneity in their source mantle.

• 대기
• /
• 제27권3호
• /
• pp.359-370
• /
• 2017

In this study, the oceanic Total Precipitable Water (TPW) retrieval algorithm at 16 km altitude of High Altitude Long Endurance Unmanned Aerial Vehicle (HALE UAV) is described. Empirical equation based on Wentz method (1995) that uses the 18.7 and 22.235 GHz channels is developed using the simulated brightness temperature and SeeBor training dataset. To do radiative simulation, Satellite Data Simulator Unit (SDSU) Radiative Transfer Model (RTM) is used. The data of 60% (523) and 40% (349) in the SeeBor training dataset are used to develop and validate the TPW retrieval algorithm, respectively. The range of coefficients for the TPW retrieval at the altitude of 3~18 km with 3 km interval were 153.69~199.87 (${\alpha}$), 54.330~58.468 (${\beta}$), and 84.519~93.484 (${\gamma}$). The bias and RMSE at each altitude were found to be about $-0.81kg\;m^{-2}$ and $2.17kg\;m^{-2}$, respectively. Correlation coefficients were more than 0.9. Radiosonde observation has been generally operated over land. To validate the accuracy of the oceanic TPW retrieval algorithm, observation data from the Korea Meteorological Administration (KMA) Gisang 1 research vessel about six clear sky cases representing spring, autumn, and summer season is used. Difference between retrieved and observed TPW at 16 km altitude were in the range of $0.53{\sim}1.87kg\;m^{-2}$, which is reasonable for most applications. Difference in TPW between retrieval and observation at each altitude (3~15 km) is also presented. Differences of TPW at altitudes more than 6 km were $0.3{\sim}1.9kg\;m^{-2}$. Retrieved TPW at 3 km altitude was smaller than upper level with a difference of $-0.25{\sim}0.75kg\;m^{-2}$ compared to the observed TPW.

• 한국대기환경학회지
• /
• 제29권6호
• /
• pp.818-829
• /
• 2013

The collection of rainwater samples was made at Jeju area during 2009~2010, and the major ionic species were analyzed. In the comparison of ion balance, conductivity, and acid fraction for the validation of analytical data, the correlation coefficients showed a good linear relationship within the range of 0.966~0.990. The volume-weighted mean pH and electric conductivity were 4.9 and $17.8{\mu}S/cm$, respectively, at the Jeju area. The volume-weighted mean concentrations of ionic species in rainwater were in the order of $Cl^-$ > $Na^+$ > $nss-SO_4{^{2-}}$ > $NH_4{^+}$ > $NO_3{^-}$ > $Mg^{2+}$ > $H^+$ > $nss-Ca^{2+}$ > $HCOO^-$ > $K^+$ > $PO_4{^{3-}}$ > $CH_3COO^-$ > $NO_2{^-}$ > $F^-$ > $HCO_3{^-}$ > $CH_3SO_3{^-}$. The ionic strength of rainwater was $0.26{\pm}0.21$ mM during the study period. The composition ratios of ionic species were such as 50.1% for the marine sources ($Na^+$, $Mg^{2+}$, $Cl^-$), 30.9% for the anthropogenic sources ($NH_4{^+}$, $nss-SO_4{^{2-}}$, $NO_3{^-}$), and 4.7% for the soil source ($nss-Ca^{2+}$), and 3.1% for organic acids ($HCOO^-$, $CH_3COO^-$). From the seasonal comparison, the concentrations of $NO_3{^-}$, $nss-Ca^{2+}$, and $nss-SO_4{^{2-}}$ increased in winter and spring seasons, indicating a reasonable possibility of long range transport from Asia continent. Especially, the acidifying contributions by major inorganic acids ($nss-SO_4{^{2-}}$ and $NO_3{^-}$) and organic acids ($HCOO^-$ and $CH_3COO^-$) were 87.6% and 12.4%, respectively. In comparison by sectional inflow pathway of air mass during the rainy sampling days, the concentrations of $nss-SO_4{^{2-}}$ and $NO_3{^-}$ were relatively high when the air mass was moved from the China continent into Jeju area.