• Macromolecular Research
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• v.16 no.1
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• pp.15-20
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• 2008

The enhanced permeability and retention (EPR) effect is used extensively for the passive targeting of many macromolecular drugs for tumors. Indeed, the EPR concept has been a gold standard in polymeric anticancer drug delivery systems. This study investigated the tumoral distribution of self-assembled nanoparticles based on the EPR effect using fluorescein and radio-labeled nanoparticles. Self-assembled nanoparticles were prepared from amphiphilic chitosan derivatives, and their tissue distribution was examined in tumor-bearing mice. The size of the nanoparticles was controlled to be 330 run, which is a size suited for opening between the defective endothelial cells in tumors. The long-circulating polymer nanoparticles were allowed to gradually accumulate in the tumors for 11 days. The amount of nanoparticles accumulated in the tumors was remarkably augmented from 3.4%ID/g tissue at 1 day to 25.9%ID/g tissue at 11 days after i.v. administration. The self-assembled nanoparticles were sustained at a high level throughout the 14 day experimental period, indicating their long systemic retention in the blood circulation. The ${\gamma}$-images provided clear evidence of selective tumor localization of the $^{131}I$-labeled nanoparticles. Confocal microscopy revealed the fluorescein-labeled nanoparticles to be preferentially localized in the perivascular regions, suggesting their extravasation to the tumors through the hyperpermeable angiogenic tumor vasculature. This highly selective tumoral accumulation of nanoparticles was attributed to the leakiness of the blood vessels in the tumors and their long residence time in the blood circulation.

• Journal of the Korean Society of Food Culture
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• v.34 no.6
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• pp.771-778
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• 2019

This study investigated the effect of temperature deviation on the water-holding capacity, tenderness, lipid oxidation, and color stability of Korean Hanwoo (韓牛) beef during long-term aging. The striploins (M. longissimus lumborum) were aged for 56 days at 2±1℃ (T1), 2±2℃ (T2), or 2±3℃ (T3). Drip loss and cooking loss were higher (p<0.05) in T3 than in T1 on day 56. The Warner-Bratzler shear force value was similar for all treatments during aging times. On day 56, the content of 2-thiobarbituric acid reactive substances was higher (p<0.05) in T3 as compared to T1. The CIE L^* and b^* values were similar for all treatments, but the a^* value was lower (p<0.05) in T3 than in T1 on the last day. These findings indicate that high temperature deviation adversely affects the water-holding capacity, lipid oxidation stability, and color stability of Hanwoo beef during long-term aging.

• Journal of Pharmacopuncture
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• v.21 no.1
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• pp.35-40
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• 2018

Objective: The role of BDNF (brain-derived neurotrophic factor), CREB (cAMP response element binding) and VGF neuropeptide has been proved in antidepressant activity of long term saffron administration in the rat hippocampus. In this study we evaluated the role of these proteins in antidepressant activity of saffron in long term administration in the rat cerebellum. Methods: Saffron aqueous extract (40 and 80 mg/kg/day) and imipramine (10 mg/kg/day) were administered intraperitoneally for 21 days to rats. At the end of experiment, animals were sacrificed and cerebellums were separated. The protein levels of BDNF, VGF, CREB and P- CREB in the rat cerebellum were evaluated using western blot analysis. Results: Saffron aqueous extract (80mg/kg/day) caused significant increase in protein level of P-CREB in long term treatment in the rat cerebellum. The increases in the protein levels of VGF, CREB and BDNF were not significant. Conclusion: In summary, our results showed that antidepressant effect of saffron in rat cerebellum might be due to the enhanced phosphorylation of CREB.

• Korean Journal of Veterinary Research
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• v.40 no.1
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• pp.1-16
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• 2000

Nowadays, mongolian gerbil is notably utilized for the research of brain and water deprivation because of a congenital incomplete willis circle structure in the brain, audiogenic seizure in low noise, and special cholesterol metabolism without water absorption for a long time. In this study, we are intend to identify the morphological changes of the catecholaminergic neuron of brain according to the time lapse in the condition of long term water deprivation. 55 mongolian gerbil were divided 10 groups(control, 1, 2, 3, 4, 5, 10, 15, 20, 42th day water deprivation group), of which each group include 5 mongolian gerbils and 5 normal mongolian gerbils in control group were also used for brain atlas as a control. The brains were observed by the immunohistochemical stain using the TH, DBH and PMNT antibody. The results were as followings; 1. The nerve fibers of the TH-immunoreactive neuron were observed only in the and corpus striatum of the telencephalon. 2. Intensity of the immunostain of the nerve fiber in the cerebral cortex and corpus striatum was decreased gradually day by day after water deprivation. 3. The TH-immunoreactive nerve cells were observed in the paraventricular and periventricular nucleus of the 3rd ventricular in the hypothalamus of mongolian gerbil but the number of nerve cells were decreased from the first day of the water deprivation to the 10th day and increased until the 20th day, after than redecreased from the 20th day by the continuous water deprivation. The number of nerve fibers in this area were increased in the first day, but decreased from the 2nd day of water deprivation. The shape and density of the dopamine secreting cells in the brain of mongolian gerbil by the immunoreactive stain were changed in the continuous water deprivation. In this results, we can conclude that dopamine concerned in the water metabolism of mongolian gerbil, and mongolian gerbil could be used as an animal model for the research of water deprivation.

• Journal of Environmental Science International
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• v.21 no.2
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• pp.189-202
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• 2012

Power spectral analysis for $PM_{10}$ observed at 10 cities in the Korean Peninsula from 2004 to 2010 was carried out to examine the spatial and temporal features of $PM_{10}$ evolution cycle. The power spectrum analysis proposed 9 typical cycles (0.5 day, 1day, 5.4day, 8~10day, 19~21day, 26day, 56day, 180day and 365day) for $PM_{10}$ evolution and the cycles are strongly associated with dilution and transportation due to the meterological influence. The spectrum intensity of 5.4day and 26day $PM_{10}$ evolution cycles mainly depend on the advection cycles of synoptic pressures system and long-term variation of climatological forcing, respectively. The intensity of $PM_{10}$ evolution with longer temporal cycles than one day tends to be stronger in La ni$\tilde{n}$a period in comparison with that in El ni$\tilde{n}$o period. Mean of typical intensity of $PM_{10}$ evolution in La ni$\tilde{n}$a period estimated to be 30% larger than El ni$\tilde{n}$o period. Thus the global scale meteorological phenomena such as El ni$\tilde{n}$o and La ni$\tilde{n}$a also can influence the variation of wind system in the Korean Peninsula and $PM_{10}$ evolution. but global scale forcing tends to influence different manner for $PM_{10}$ evolution in accordance with its temporal cycles.

• Journal of The Korean Society of Integrative Medicine
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• v.8 no.1
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• pp.219-228
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• 2020

Purpose : This study highlights the importance of occupational therapy at adult day care centers by examining the current state of this therapy at these centers. Methods : Data on 1,527 adult day care centers in South Korea were retrieved from a long-term care insurance site for the elderly and were statistically processed. Results : Of these centers, 1,331 (87.2 %) were cognitive activity-oriented, 37 (2.4 %) were exclusively for dementia treatment, and 159 were nonspecialized centers (10.4 %). A second finding was that 294 (19.3 %) of the centers operated occupational therapy programs. Third, only 40 occupational therapists (2.6 %) were working at one of these 1,527 care centers nationwide. Fourth, the occupational therapy programs comprised 4.91 ± 3.93 cognitive enhancement programs per center, 2.58 ± 2.26 training assistance programs per center, and other programs 2.56 ± 2.58 per center. Fifth, a regional comparison of the operation of occupational therapy programs at these centers showed a statistically significant difference in the number of programs between Seoul and other regions. Conclusion : Although most adult day care centers currently deliver cognitive enhancement programs, they show a low employment rate of occupational therapists. Therefore, additional research involving workers at adult day care centers is required to investigate perceptions and the necessity of occupational therapy.

• Journal of Korea Soil Environment Society
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• v.1 no.1
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• pp.19-27
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• 1996

Nonaqueous phase liquids(NAPL) not readily dissolved in water exist as a separate fluid phase. Groundwater contamination by NAPL such as organic solvents and petroleum hydrocarbons becomes major public concerns because of their long-term persistence in the subsurFace and their ability to contaminate large volumes of wate. Dense.-than-water NAPL(DNAPL) spilled into the subsurface penetrate through the saturated zone and ultimately form DNAPL pools on the bottom of the aquifer. The dissolution of DNAPL from these pools depends on the molecular diffusion coefficient, the vertical dispersivity, the groundwater velocity, the solubility, and the pool length. In this study, the vertical transverse dispersion coefficients for simulating the dissolution of DNAPL from such pools were obtained from the dissolution experiment. Under the experimental conditions used, the vertical transverse dispersion coefficients calculated were 1.86$cm^2$/day, 2.90$cm^2$/day and 4.51$cm^2$/4ay for seepage velocities of 59.2cm/day, 94.3cm/day and 158.0cm/day, respectively. And the vertical transverse dispersivity was 0.03024cm.

• Biomedical Science Letters
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• v.24 no.4
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• pp.411-417
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• 2018

The aim of this study was to investigate the impact of participation in the 622 km hyper-ultra-marathon on hepatic metabolism and renal function in middle-aged men. Healthy middle-aged male amateur ultra-marathoners between the ages of 40 and 60. Blood was collected at the pre-race, immediately after 300 km, 622 km hyper-ultra marathon race, 72 hours (3 day) and 144 hours (6 day) after the race, AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALP (alkaline phosphatase), ${\gamma}$-GTP (gamma glutamyl transferase), T-Bil (total bilirubin), D-Bil (direct bilirubin), T-protein (total protein), albumin, uric acid, BUN (blood urea nitrogen), creatinne were analyzed. ALP was significantly increased at 300 km, 622 km, day 3 and day 6 than the pre-race. ${\gamma}$-GTP, T-protein, albumin, uric acid, BUN and creatinine were not significantly different between the distances and the recovery period respectively. AST and ALT were significantly increased at 300 km, 622 km, day 3 and day 6 than the pre-race, respectively (P<0.05) at day 3 and day 6 they showed significant decrease from 300 km and 622 km, respectively (P<0.05). T-Bil and D-Bil increased significantly at 300 km and 622 km, respectively (P<.05) and significantly decreased at day 3 (P<0.05) compared to the pre-race, at day 3 and day 6 they were decreased significantly than 300 km and 622 km, respectively (P<0.05). In conclusion, no disturbance of renal function was observed according to the distances and between the recovery period of 622 km hyper-ultra marathon race, but reversible hepatocyte function could be degraded and some hemolysis of blood vessels was induced.

• Journal of The Korean Astronomical Society
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• v.11 no.1
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• pp.47-54
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• 1978

Twelve Chih was sasigned to each day in the old Chinese calendar. This paper clarifres the relations between 12 Chih and the diurnal motion of Big Dipper or the clock, and it shows the long term table and formula for the dayly assignment of 12 Chih in the calendar.

• The Journal of Engineering Geology
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• v.20 no.2
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• pp.169-181
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• 2010

This study aimed at simulating several responses to stresses caused by the ground water level variations around the K-1 oil stockpile. For this simulation, we considered the characteristic hydrogeological condition including the special occurrence of long and thick acidic dyke, which is regarded as the main geological structure dominating the ground water flow system at this study area. We activated twenty-four imaginary wells which are located in northern and southern area around central K-1 site. Each neighboring distance is altogether 300 m and whole distance between K-1 site and remote wells is 1,200 m. Through the modeling, we operated the long-term and continuous pumping tests and finally categorized five zones based on maximum pumping rates for the imaginary wells; zone I within 300 meter distance from K-1 site with a pumping rate of 50 $m^3/day$; zone II between 300 to 600 meter distance from K-1 site with a pumping rate of 75 $m^3/day$; zone III between 600 to 900 meter distance from K-1 site with 150 $m^3/day$; zone IV between 900 to 1,200 meter distance from K-1 site with 300 $m^3/day$; and zone V of acidic dyke area. At zone V, especially because of their possibility of high transmissivity for groundwater flow, it is necessary to control and restrict groundwater discharge.