• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1637-1642
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• 2015

Background: RhoGTPase-activating proteins (RhoGAPs) regulate RhoGTPases in cells, but whether individual reactive oxygen species (ROS) regulate RhoGAPs is unknown. Our previous published papers have shown that deleted in liver cancer 1 (DLC1) inhibits cancer cell migration by its RhoGAP activity. The present study was designed to explore the role of $H_2O_2$ in regulation of DLC1. Materials and Methods: We treated cells with $H_2O_2$ for 24h and phenotypic changes were analyzed by MTT, RT-PCR, Western blotting, immunofluorescence staining and wound healing assays. Results: $H_2O_2$ downregulated cyclin D1 and cyclin E to inhibit proliferation, and upregulated BAX to induce apoptosis in MCF-7 cells. Compared with non-tumorigenic cells, $H_2O_2$ increased expression of DLC1 and reduced activity of RhoA in cancer cells. Stress fiber production and migration were also suppressed by $H_2O_2$ in MDA-MB-231 cells. Conclusions: Our study suggests that $H_2O_2$ inhibits proliferation through modulation of cell cycle and apoptosis-related genes, and inhibits migration by decreasing stress fibers via DLC1/RhoA signaling.

• Korean Journal of Environmental Biology
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• v.25 no.3
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• pp.191-196
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• 2007

Indoor air differs from outdoor atmosphere since it contains chemical and physical contaminants from building materials. This study deals with the biological effects of volatile organic compounds (VOCs) released from synthetic fiber carpet materials. One group of Tradescantia inflorescence was exposed to VOCs from the carpet sample in the environmental test chamber, while the other inflorescence group was exposed to a TO-14 standard gas mixture (1 ppm) for comparison. After the exposure, VOCs from the carpet were analysed by the desorber/GC/MS method, and micronuclei in the pollen mother cells of Tradescantia were scored under a microscope $({\times}400)$ to evaluate the genotocixicity induced by the exposure to VOCs. The chemical analysis confirmed that a total of 12 VOCs were released from the carpet materials, among which stylene $(71.9{\mu}g\;m^{-3})$ and toluene $(49.6{\mu}g\;m^{-3})$ were in the highest concentration. Twenty four hours of exposure to VOCs from the carpet in the environmental test chamber resulted in a micronucleus frequency as high as $7.73{\pm}0.75MCN$ per 100 tetrads, which was similar to that induced after exposure to the TO-14 standard gas mixture (1 ppm) for 4 hours. Meanwhile, two hours of exposure to the standard gas mixture did not cause a significant increase in the genotoxicity compared to the spontaneous micronucleus frequency. This result indicates that exposure for a long time to the air contaminated with VOCs from the carpet materials causes a genotoxic effect. The biological-chemical combination analyses in the study proved to be an effective tool for monitoring the indoor air contaminants.

• Proceedings of the KWS Conference
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• 2009.11a
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• pp.29-29
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• 2009

On this research, laser welding technology for manufacturing automobile body is studied. Laser welding technology is one of the important technologies used in the manufacturing of lighter, safer automotive bodies at a high level of productivity; the leading automotive manufacturers have replaced spot welding with laser welding in the process of car body assembly. Korean auto manufacturers are developing and applying the laser welding technology using a high output power Nd:YAG laser and a 6-axes industrial robot. On the other hand, the robot-based remote laser welding system was equipped with a long focal laser scanner system in robotic end effect. Laser system, robot system, and scanner system are used for realizing the high speed laser welding system. The remote laser welding system and industrial robotic system are used to consist of robot-based remote laser welding system. The robot-based remote laser welding system is flexible and able to improve laser welding speed compared with traditional welding as spot welding and laser welding. The robot-based remote laser systems used in this study were Trumpf's 4kW Nd:YAG laser (HL4006D) and IPG's 1.6kW Fiber laser (YLR-1600), while the robot systems were of ABB's IRB6400R (payload:120kg) and Hyundai Heavy Industry's HX130-02 (payload:130kg). In addition, a study of quality evaluation and monitoring technology for the remote laser welding was conducted. The welding joints of steel plate and steel plate coated with zinc were butt and lapped joints. The quality testing of the laser welding was conducted by observing the shape of the beads on the plate and the cross-section of the welded parts, analyzing the results of mechanical tension test, and monitoring the plasma intensity and temperature by using UV and IR detectors. Over the past years, Trumf's 4kW Nd:YAG laser and ABB's IRB6400R robot system was used. Nowadays, the new laser source, robot and laser scanner system are used to increase the processing speed and to improve the efficiency of processes. This paper proposes the robot-based remote laser welding system as a means of resolving the limited welding speed and accuracy of conventional laser welding systems.

• Korean Journal of Community Nutrition
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• v.25 no.5
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• pp.406-415
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• 2020

Objectives: The aim of this study was to compare the nutritional intake and Diet Quality Index-International (DQI-I) of gynecological cancer survivors and normal women. Methods: This study compared the anthropometric indices, dietary behavior, nutritional intake, and DQI-I in women with previous history of breast or uterine cancer [Gynecological cancer survivors group (GCSG, n=126)] and normal women [Normal control group (NCG, n=7,011)] using the 2013~2016 Korea National Health and Nutrition Examination Survey data. Results: Body mass index and waist circumference were lower in the GCSG compared the NCG. The frequency of skipping breakfast and eating out was higher in the NCG compared to GCSG. Energy and fat intake were significantly higher in the NCG than in the GCSG, whereas intake of all minerals and vitamins (excluding thiamine), and dietary fiber intake were higher in GCSG. It was observed that the fatty acid intake of the GCSG was significantly lower than that of the NCG. The diet quality evaluation using DQI-I results showed that GCSG was higher in the "within-group" diet variety and adequacy of vegetable group than the NCG, whereas the intake level of the fruit group was higher in NCG. Besides, protein, calcium, and vitamin C intake were higher in the GCSG than in the NCG. The GCSG showed higher levels of total fat and saturated fat moderation than the NCG, whereas cholesterol moderation showed the opposite results. The results of DQI-I comparison according to the cancer survival years showed that the overall score and scores related to diet adequacy and balance were higher in the below 5-year group, whereas the over 5-year group scored higher in terms of moderation of diet. Conclusions: The results of this study suggest that a chronic disease based management approach is needed in cancer survivors. The study provides important data which can help in the preparation of guidelines for long-term lifestyle and diet management, in these patients.

• Journal of Korean Society of Disaster and Security
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• v.13 no.1
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• pp.13-23
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• 2020

In 2005, Urban railway seismic design was introduced in Rep. Of Korea, and many studies on seismic performance evaluation and reinforcement methods were conducted. In accordance with the Enforcement Decree of the Earthquake Disaster Countermeasures Act issued in March 2009, during April 2010 to October 2013, some of local governments established detailed evaluation and reinforcement measures for seismic performance of the urban railway underground structure. Afterwards, the seismic performance reinforcement of the existing urban railway structures was conducted for the sections that a long period of used until the end of 2018, and the reinforcement works are carried out by various methods using the previously studied methods. However, various reinforcing materials and construction methods using have been studied, but the classification research on the construction methods currently applied to reinforcement construction of urban railways is insufficient. The purpose of study is to analysis the cases currently applied to seismic reinforcement construction and to show the characteristics of each construction method, the reasons for its application and problems.

• Information and Communications Magazine
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• v.14 no.12
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• pp.77-85
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• 1997

지금까지 access network에서의 xDSL의 위치를 살펴보고 DSL, HDSL, ADSL 및 VDSL을 중심으로 기술 및 최근의 표준화동향을 살펴보았다. ITU의 조사에 따르면 97년 기준으로 전세계 약 8억가구에 copper access line이 연결되어 있고 그중의 70%이상이 가정에 연결되어 있는데 증가추세도 북미의 경우 년3.5%, 서유럽과 태평양연안의 경우 년4.5%의 성장율을 보이고 있어 2000년경에는 10억가구에 다다를 전망이다. 미국은 97년기준 약 1억가구에 1억6000만 copper access line이 연결되어 있는데 Yankee Group이 조사한 바에 따르면 2000년을 기준으로 3백만가구에 ADSL/RADSL/SDSL이 연결되고 관련 revenue는 10억달러에 이를것으로 추산하고 있다. 전세계 xDSL 시장규모를 미국의 5배정도로 보면 2000년경에는 전세계 twist pair의 2%정도에 xDSL이 연결되어 ADSL/RADSL/SDSL 관련 세계시장규모가 50억달러에 이르고 시장성장률은 30%에 이를 것으로 보고 있다. 현재 이러한 xDSL의 표준화작업은 3장에서 언급한 바와 같이 ANSI T1, ETSI에서 주로 주도되고 있으나 ITU-T, IEEE, ADSL Forum, ATM Forum, DAVIC 등 다른 group과 활발한 liasion meeting 을 통해서도 협력안을 마련하려 노력하고 있다. 예를 들어 DAVIC은 long range(1.5Km이상) PMD규격으로 ADSL을, mid range($300m\sim1.5km$) PMD규격으로 VDSL을, short range(300m이하) PMD규격으로 CAP방식의 FTTC를 각각구정하여 xDSL을 수용하고 있다. Microsoft의 CEO인 Bill Gates가 'Bandwidth bottleneck. No question. that's the biggest obstacle.'이라고 천명한 바와 같이 정보사회로의 진전에 있어 bandwidth는 시급히 해결되어야 할 문제이다. 정보사회의 꿈을 실현하고자 1993년부터 시작된 In-formation Infrastructure의 구축노력이 그동안 다양하게 시도되어왔으나 숱한 우여곡절과 실패를 겪은 telco등의 서비스업자들이 이제는 너무 큰 꿈을 쫓기보다는 internet이라는 현실적인 시장에서 xDSL과 cablemodem을 바탕으로 차근차근 새로 시작하고 있다고 생각된다. 그동안 ADSL, Forum을 중심으로한 일부 technology evangelist들은 전세계 8억가구에 깔려있는 copper twist pair를 경제적인 'the last mile solution'으로 활용할 것을 줄기차게 주장해왔다. 그동안 xDSL은 VDSL이라는 차세대 대안을 제시하여 interim solution이라는 울타리를 벗어나는데 성공하였을 뿐만 아니라 IDSL, SDSL, RADSL등의 새로운 고객지향적 xDSL을 개발하여 선택의 폭을 넓혀주었다. xDSL vendor들간의 치열한 경쟁속에서 값싼 xDSL 시스템들이 속속 등장하고 있으며, DSLAM[8]과 같은 switch solution도 제시되어 기존의 copper twist pair를 이용해 값싸게 다양한서비스를 제공해 줄 수 있는 기틀을 마련한 것이 오늘날의 xDSL의 성공요인이라고 생각된다. 일찌기 Bell Atlantic의 CEO Ray Smith가 'Yes. ADSL is an interim solution that will be with us for forty years.'라고 간파한대로 xDSL은 fiber가 좀 더 값싼 해결책이 될 때까지 당분간 access network에서 확고한 자리를 구축할 것으로 보인다. 최근들어 우리나라의 초고속정보통신망도 ADSL 과 VDSL을 바탕으로 FTTH으로 진화하는 전략을 수립한것은 우리의 현실과 세계적인 추세를 반영한 일이라 여겨진다.

• Macromolecular Research
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• v.11 no.4
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• pp.207-223
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• 2003

For last five years, we are developing the novel local drug delivery devices using biodegradable polymers, especially polylactide (PLA) and poly(D,L-lactide-co-glycolide) (PLGA) due to its relatively good biocompatibility, easily controlled biodegradability, good processability and only FDA approved synthetic degradable polymers. The relationship between various kinds of drug [water soluble small molecule drugs: gentamicin sulfate (GS), fentanyl citrate (FC), BCNU, azidothymidine (AZT), pamidronate (ADP), $1,25(OH)_2$ vitamin $D_3$, water insoluble small molecule drugs: fentanyl, ipriflavone (IP) and nifedipine, and water soluble large peptide molecule drug: nerve growth factor (NGF), and Japanese encephalitis virus (JEV)], different types of geometrical devices [microspheres (MSs), microcapsule, nanoparticle, wafers, pellet, beads, multiple-layered beads, implants, fiber, scaffolds, and films], and pharmacological activity are proposed and discussed for the application of pharmaceutics and tissue engineering. Also, local drug delivery devices proposed in this work are introduced in view of preparation method, drug release behavior, biocompatibility, pharmacological effect, and animal studies. In conclusion, we can control the drug release profiles varying with the preparation, formulation and geometrical parameters. Moreover, any types of drug were successfully applicable to achieve linear sustained release from short period ($1{\sim}3$ days) to long period (over 2 months). It is very important to design a suitable formulation for the wanting period of bioactive molecules loaded in biodegradable polymers for the local delivery of drug. The drug release is affected by many factors such as hydrophilicity of drug, electric charge of drug, drug loading amount, polymer molecular weight, the monomer composition, the size of implants, the applied fabrication techniques, and so on. It is well known that the commercialization of new drug needs a lot of cost of money (average: over 10 million US dollar per one drug) and time (average: above 9 years) whereas the development of DDS and high effective generic drug might be need relatively low investment with a short time period. Also, one core technology of DDS can be applicable to many drugs for the market needs. From these reasons, the DDS research on potent generic drugs might be suitable for less risk and high return.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.586-590
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• 2009

Horsehair worms (Chordodes koreensis) develop as parasites in the bodies of grasshoppers, crickets, cockroaches, and some beetles. Chordodes koreensis is an accidental parasite of humans, livestock, or pets and poses no public health threat. The male of Chordodes koreensis in the later larval stage from canine vomitus was investigated by the scanning and transmission electron microscopy. In cross sections, the body wall is composed of four components namely epicuticle, cuticle, epidermis, and muscle layers. The parenchymal tissue fills the rest of the body and surrounds the visceral organs such as intestine, and ventral nerve cord but testes were not found. The epicuticle is a thin superficial layer whose surface shows rows of polygonal elevations called areoles. The cuticle has 17 layers of collagenous fibers spirally wound about the long axis of the worm. The section through the cuticle reveals the layers of large fibers cut obliquely lengthwise, alternating with layers of fibers sectioned obliquely crosswise. The layers of large fiber formed a double helix about longitudinal axis of the worm. The epidermis is a single layer. The muscles were interrupted by the nervous lamella in the only midventral portion. The medulla of muscle plate is composed of lightly stained cytoplasm, mitochondria, weakly developed endoplasmic reticulum, and glycogen granules. Between the medulla of a cell and the plasmalemma lies a broad cortical zone of myofilaments. The circular muscles are absent. The characteristic feature of the cytoplasm is that there was no content in peripheral mesenchyme, but was an abundance of large clear vacuoles which give the cytosome a foamy appearance. The nucleus of mesenchyme is not easily identified in our specimens.

• Journal of Periodontal and Implant Science
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• v.36 no.1
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• pp.27-37
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• 2006

Although the main purpose of periodontal treatment to regenerate is the complete regeneration of periodontal tissue due to periodontal disease, most of the treatment cannot meet such purpose because healing by long epithelial junction. Therefore, diverse materials of resorbable and non-resorbable have been used to regenerate the periodontal tissue. Due to high risk of exposure and necessity of secondary surgical procedure when using non-resorbable membrane, guided tissue regeneration using the resorbable membrane has gain popularity, recently. However, present resorbable membrane has the disadvantage of not having sufficient time to regenerate date to the difference of resorption rate according to surgical site. Meanwhile, other than the structure stability and facile manipulation, acellular dermal matrix has been reported to be a possible scaffold for cellular proliferation due to rapid revascularization and favorable physical properties for cellular attachment and proliferation. The purpose of this study is to estimate the influence of acellular dermal matrix on periodontal ligament, cementum and alveolar bone when acellular dermal matrix is implanted to 1-wall alveolar bone defect. 4 dogs of 12 to 16 month old irrelevant to sex , which below 15Kg of body weight, has been used in this study. ADM has been used for the material of guided tissue regeneration. The 3rd premolar of the lower jaw was extracted bilaterally and awaited for self-healing. subsequently buccal and lingual flap was elevated to form one wall intrabony defect with the depth and width of 4mm on the distal surface of 2nd premolar and the mesial surface of 4th premolar. After the removal of periodontal ligament by root planing. notch was formed on the basal position. Following the root surface treatment, while the control group had the flap sutured without any treatment on surgically induced intrabony defect. Following the root surface treatment, the flap of intrabony defect was sutured with the ADM inserted while the control group sutured without any insertion. The histologic specimen was observed after 4 and 8 weeks of treatment. The control group was partially regenerated by periodontal ligament, new cementum and new alveolar bone. the level of regeneration is not reached on the previous formed notch. but, experimental group was fully regenerated by functionally oriented periodontal ligament fiber. new cementum and new alveolar bone. In conclusion, we think that ADM seems to be used by scaffold for periodontal ligament cells and the matrix is expected to use on guided tissue regeneration.

• Korean Journal of Optics and Photonics
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• v.10 no.3
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• pp.195-200
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• 1999

We made sharp optical fiber tips with less than 100 nm diameter by using the heating and pulling method with a good repetition and fabricated the photon scanning tunneling microscope (PSTM) using constant intensity mode. The 3-dimensional PZT (Piezoelctric transducer) scanner made of a long PZT tube is consisted of three divided parts, that is, a pair of $\pm$ x and a pair of $\pm$y scanning parts and a z scanning part for the fine approach and scanning. The scanning dimension is 1.43 $\mu\textrm{m}$$\times$1.76 $\mu\textrm{m}$. The height of a optical tip to maintain a constant height within $1/{\lambda}_0$ (${\lambda}_0$ is the incident wavelength) from surface of a specimen to a optical tip is controlled automatically by using the electric feedback circuit. The 3-dimensional shape of standing evanescent waves generated on the surface of a dove prism was measured successfully by using the constant intensity mode PSTM.