• Molecules and Cells
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• 제39권12호
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• pp.841-846
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• 2016

Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translation in live neurons. Among various techniques for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in neurons. Here, we review recent advances in the study of mRNA localization and translation in live neurons using novel techniques for single-RNA imaging.

• Nuclear Medicine and Molecular Imaging
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• 제41권2호
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• pp.97-101
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• 2007

Correct localization of epileptogenic zone is important for the successful epilepsy surgery. Both ictal perfusion single photon emission computed tomography (SPECT) and interictal F-18 fluorodeoxyglucose positron emission tomography (FDG-PET) can provide useful information in the presurgical localization of intractable partial epilepsy. These imaging modalities have excellent diagnostic sensitivity in medial temporal lobe epilepsy and provide good presurgical information in neocortical epilepsy. Also provide functional information about cellular functions to better understand the neurobiology of epilepsy and to better define the ictal onset zone, symptomatogenic zone, propagation pathways, functional deficit zone and surround inhibition zones. Multimodality imaging and developments in analysis methods of ictal perfusion SPECT and new PET ligand other than FDG help to better define the localization.

• 대한의용생체공학회:의공학회지
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• 제18권1호
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• pp.9-16
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• 1997

We develop an automatic imaging matching technique for combining portal image and simulator image for improvements in localization of treatment in radiation therapy. Fusion of images from two imaging modalities is treated as follows. We archive images thxough a frame-yabber. The simulator and portal images are edge detected and enhanced with interpolated adaptive histouam equalization and combined using geometrical parameters relating the coordinates of two image data sets which are calculated using Fourier descriptors. We don't use any kind of imaging markers for patient's convenience. clinical use of this image matching technique for treatment planning will result in improvements in localization of treatment volumes and critical structures. These improvements will allow greater sparing of normal tissues and more precise delivery of energy to the desired irradiation volume.

• 로봇학회논문지
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• 제9권4호
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• pp.232-241
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• 2014

This paper proposes an underwater localization algorithm using probabilistic object recognition. It is organized as follows; 1) recognizing artificial objects using imaging sonar, and 2) localizing the recognized objects and the vehicle using EKF(Extended Kalman Filter) based SLAM. For this purpose, we develop artificial landmarks to be recognized even under the unstable sonar images induced by noise. Moreover, a probabilistic recognition framework is proposed. In this way, the distance and bearing of the recognized artificial landmarks are acquired to perform the localization of the underwater vehicle. Using the recognized objects, EKF-based SLAM is carried out and results in a path of the underwater vehicle and the location of landmarks. The proposed localization algorithm is verified by experiments in a basin.

• Investigative Magnetic Resonance Imaging
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• 제15권1호
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• pp.11-21
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• 2011

목적: 금속전극은 MRI 안에서 자기장의 왜곡을 일으켜 영상에 인공물이 나타난다. 본 논문에서는 전극이 B0와 수직으로 놓였을때 자기장 패턴의 특성을 이용하여 oblique-view angle imaging 방식을 통해 전극의 정확한 위치를 결정하는 방법을 제시하고자 한다. 대상 및 방법: 다양한 직경과 자화율을 가진 금속 전극모델의 시뮬레이션을 통하여 전극으로 인해 왜곡되는 field map의 양상을 파악하고 해상도에 따른 turbo spin-echo (TSE) 영상의 왜곡패턴을 분석하여 일반적인 영상기 법($90^{\circ}$ view)과 $45^{\circ}$ oblique-view에서의 위치 추정 기준을 마련하였으며 3.0T 임상용 장비에서 실제 전극의 TSE영상을 획득하여 시뮬레이션과 대조 검증하였다. 상대적으로 자기장의 왜곡에 민감한 gradient-refocused echo (GRE)시퀀스에서는 위상 영상을 이용해 위치를 추정하였다. 결과: 금속전극이 B0와 수직일 때 전극을 통과하는 $45^{\circ}$ 선상에서는 자기장 패턴의 변화가 매우 적었다. TSE 시퀀스의 경우 $45^{\circ}$ oblique-view 영상에서는 자화율의 크기에 관계없이 위치 추정기준이 잘 들어 맞았으며 자기장 왜곡에 의한 픽셀이 동양상이 양방향 대칭적으로 일어나므로 해상도가 낮은 경우에도 정확한 위치 추정이 가능하였다. 또한 GRE 시퀀스를 사용하였을때 $45^{\circ}$ oblique-view에서는 위상의 극성이 변화하는 선이 직교좌표계와 일치하기 때문에 일반적 방법보다 위치추정이 용이하였다. 결론: 시뮬레이션과 실제영상을 이용하여 일반적인 $90^{\circ}$ view에서보다 $45^{\circ}$ oblique-view에서 금속전극의 위치추정이 용이함을 확인하였다. 이는 전기 생리학적인 뇌연구 및 뇌수술 등을 MRI로 모니터링 하는데 적용 가능할 것으로 기대된다.

• International journal of thyroidology
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• 제11권2호
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• pp.92-98
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• 2018

Intraoperative identification and localization of parathyroid glands are crucial step in preventing postoperative hypocalcemia during thyroid and parathyroid surgery. If there is a method to predict the parathyroid's location rather than detecting and verifying with naked eye, it would make the operator easier to find and identify the parathyroid. Recently, near-infrared light imaging technologies have been introduced in the fields of thyroid and parathyroid surgery to predict the localization of the parathyroid. These are being conducted in two ways: autofluorescence imaging with a unique intrinsic fluorophore in the parathyroid tissues and fluorescence imaging with external fluorescence materials specially absorbed into parathyroid tissues. We are suggest that parathyroid glands can be detected by surgeon with NIR autofluorescence imaging even if they are covered by fibrofatty tissues before they are detected by surgeon's naked eye. These novel techniques are very useful to identify and preserve parathyroid glands during thyroidectomy. In this article, we reviewed the latest papers that describe autofluorescence imaging and exogenous ICG fluorescence imaging of parathyroid glands during thyroid and parathyroid surgery.

• 비파괴검사학회지
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• 제32권6호
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• pp.703-710
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• 2012

A structural health monitoring technique for locating impact position in a composite plate is presented in this paper. The method employs a single sensor and spatial focusing properties of time reversal(TR) and inverse filtering(IF). We first examine the spatial focusing efficiency of both approaches at the impact position and its surroundings through impact experiments. The imaging results of impact localization show that the impact location can be accurately estimated in any position of the plate. Compared to existing techniques for locating impact or acoustic emission source, the proposed method has the benefits of using a single sensor and not requiring knowledge of anisotropic material properties and geometry of structures. Furthermore, it does not depend on a particular mode of dispersive Lamb waves that is frequently used in other ultrasonic testing of plate-like structures.

• The Journal of the Acoustical Society of Korea
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• 제18권2E호
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• pp.10-17
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• 1999

The aim of the work described in this paper is to develop a complex underground acoustic system which detects and localizes the origin of an underground hammering sound using an array of hydrophones located about 100m underground. Three different methods for the sound localization will be presented, a time-delay method, a power-attenuation method and a hybrid method. In the time-delay method, the cross correlation of the signals received from the array of sensors is used to calculate the time delays between those signals. In the power-attenuation method, the powers of the received signals provide a measure of the distances of the source from the sensors. In the hybrid method, both informations of time-delays and power-ratios are coupled together to produce better performance of position estimation. A new acoustic imaging technique has been developed for improving the hybrid method. This new acoustic imaging method shows the multi-dimensional distribution of the normalized cost function, so as to indicate the trend of the minimizing direction toward the source location. For each method the sound localization is carried out in three dimensions underground. The distance between the true and estimated origins of the source is 28m for a search area of radius 250m.

• Asian Pacific Journal of Cancer Prevention
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• 제16권5호
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• pp.1715-1718
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• 2015

Background: Magnetic resonance imaging of breast, reported to be a high sensitivity of 94% to 100%, is the most sensitive method for detection of breast cancer. The purpose of this study was to investigate our clinical experience in MRI-guided breast lesion wire localization in Chinese women. Materials and Methods: A total of 44 patients with 46 lesions undergoing MRI-guided breast lesion localization were prospectively entered into this study between November 2013 and September 2014. Samples were collected using a 1.5-T magnet with a special MR biopsy positioning frame device. We evaluated clinical lesion characteristics on pre-biopsy MRI, pathologic results, and dynamic curve type baseline analysis. Results: Of the total of 46 wire localization excision biopsied lesions carried out in 44 female patients, pathology revealed fourteen malignancies (14/46, 30.4%) and thirty-two benign lesions (32/46, 69.6%). All lesions were successfully localized followed by excision biopsy and assessed for morphologic features highly suggestive of malignancy according to the American College of Radiology Breast Imaging Reporting and Data System (BI-RADS) category of MRI (C4a=18, C4b=17, C4c=8,C5=3). Of 46 lesions, 37 were masses and 9 were non-mass enhancement lesions. Thirty-two lesions showed a continuous kinetics curve, 11 were plateau and 3 were washout. Conclusions: Our study showed success in MRI-guided breast lesion wire localization with a satisfactory cancer diagnosis rate of 30.4%. MRI-guided wire localization breast lesion open biopsy is a safe and effective tool for the workup of suspicious lesions seen on breast MRI alone without major complications. This may contribute to increasing the diagnosis rate of early breast cancer and improve the prognosis in Chinese women.

• 한국게임학회 논문지
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• 제12권3호
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• pp.87-96
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• 2012

본 논문에서는 시퀀스 상에서 확장 칼만필터(Extended Kalman Filter) 기반의 SLAM(Simultaneous Localization And Mapping) 시스템의 안정적인 카메라 추적과 재위치(re-localization) 방법이 제안된다. SLAM으로 얻어진 3차원 특징점에 들로네(Delaunay) 삼각화를 적용하여 기준(reference) 평면을 설정하며, 평면상에 존재하는 특징점의 BRISK(Binary Robust Invariant Scalable Keypoints) 기술자(descriptor)를 생성한다. 기존 확장 칼만필터의 오차가 누적되는 경우를 판단하여 기준 평면의 호모그래피로부터 카메라 정보를 해석한다. 또한 카메라가 급격하게 이동해서 특징점 추적이 실패하면, 저장된 강건한 기술자 정보를 매칭하여 카메라의 위치를 다시 추정한다.