• 한국농촌간호학회지
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• 제16권2호
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• pp.29-36
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• 2021

Purpose: The purpose of this study is to develop a living lab program to solve the musculoskeletal health problems of rural women and analyze its effects. Methods: The subjects included eight rural women and this study involved pretest and posttest designs for a single group. The program ran from July to August 2020 and consisted of one in-person training session and three weeks of management. The effectiveness of the program was evaluated by the change in the degree of pain experienced in the wrists, shoulders, and back, along with the general health status of the subjects. The Wilcoxon Sign-Rank test was used in the analysis. In addition, the program satisfaction was analyzed with five items based on the factors of the health belief model. Results: While the program seemed to have no significant impact on the health status of the subjects, all the participants did report reduced pain in their wrists, shoulders, and lower back. The 'sensitivity' and 'cue to action' metrics also increased with participation in the program. Conclusion: This program was effective in relieving some pain associated with the musculoskeletal problems in rural women. Therefore, such programs should be sustained and spread around community organizations

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.468-472
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• 2003

Recently, genetic engineering techniques have been used to display various heterologous peptides and proteins (enzyme, antibody, antigen, receptor and fluorescence protein, etc.) on the yeast cell surface. Living cells displaying various enzymes on their surface could be used repeatedly as 'whole cell biocatalysts' like immobilized enzymes. We constructed a yeast based whole cell biocatalyst displaying T. reesei cellobiohydrolase I (CBH I ) on the cell surface and endowed the yeast-cells with the ability to degrade cellulose. By using a cell surface engineering system based on ${\alpha}-agglutinin,$ CBH I was displayed on the cell surface as a fusion protein containing the N-terminal leader peptide encoding a Gly-Ser linker and the $Xpress^{TM}$ epitope. Localization of the fusion protein on the cell surface was confirmed by confocal microscopy. In this study, we report on the genetic immobilization of T. reesei CBH I on the S. cerevisiae and hydrolytic activity of cell surface displayed CBH I.

• 한국기술혁신학회:학술대회논문집
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• 한국기술혁신학회 2015년도 추계학술대회 논문집
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• pp.907-926
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• 2015

현재 우리나라 혁신시스템은 기존의 성장 및 기술공급 위주의 발전전략이 한계를 노정하면서 새로운 전환기를 맞고 있다. 이에 새로운 혁신 경로 창출을 위한 과학기술 ICT 정책 패러다임 전환은 물론 지속가능한 에너지, 농업 농촌 등 다양한 영역에서의 시스템 전환이 필요한 상황이다. 이를 위해서는 과학기술 ICT와 에너지 환경 등 관련 분야 간의 연계 통합은 물론, 기술이 활용 확산되는 현장이나 사회, 사용자의 이해가 더욱 중요해진다. 리빙랩은 사용자 참여를 기반으로 하는 새로운 혁신모델이자 지속가능한 시스템 전환의 니치 실험으로서 최근 유럽을 중심으로 부각되고 있다. 본 연구에서는 과학기술 ICT가 에너지 전환, 농업 농촌 시스템 전환, ICT 정책 패러다임의 전환에 적용된 리빙랩 사례를 살펴보고 우리나라에서의 시사점을 도출하였다. 유럽의 SusLab 프로젝트, C@R 프로그램, 대만의 리빙랩 사례 모두 사전기획 단계부터 지역 사용자의 배경과 경험을 적극적으로 수집하고, 사용자 주도의 혁신활동을 개발프로세스 전반에서 유지함으로써 적실성있는 문제해결은 물론 다양한 전환 실험을 성공적으로 추진하였다. 이러한 결과는 중앙정부와 지자체의 R&D사업에서 각각 리빙랩의 도입을 검토하는 우리나라에 다양한 시사점을 제공한다. 리빙랩은 국가 또는 지역 전반의 사회 기술시스템의 전환을 위한 전략적 니치 실험, 정책 간 연계통합의 수단, 새로운 지역혁신 모델로 활용될 수 있다. 또한 최근 혁신정책 패러다임에서 강조하고 있는 사용자 및 수요 측면을 반영한 정책통합을 실현할 수 있는 중요한 플랫폼이 될 수 있다.

• 한국축산식품학회지
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• 제41권2호
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• pp.300-311
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• 2021

The objective of the present study was to evaluate the cholesterol-assimilation ability of lactic acid bacteria (LAB), which were isolated from kimchi, a Korean traditional fermented cabbage. The isolated strain, using modified MRS medium, showed 30.5% cholesterol assimilation activity and was named Pediococcus acidilactici LRCC5307. Types and concentrations of bile were investigated for their effects on increasing the cholesterol assimilation ability of the LRCC5307 strain, a 74.5% decrease in cholesterol was observed when 0.2% bile salts were added. In addition, the manufacture of low-cholesterol butter using LRCC5307 was examined. After fermentation, LRCC5307 with butter showed 8.74 Log CFU/g viable cells, pH 5.43, and a 11% decrease in cholesterol. These results suggest that LRCC5307 could help in the production of healthier butter by decreasing cholesterol and including living LAB.

• 한국식품위생안전성학회지
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• 제20권4호
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• pp.267-271
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• 2005

본 연구는 세포막의 구성성분으로서 인체의 신진대사에 관여하는 물질인 인지질을 정량하기 위하여 인지질의 주요성분인 포스파티딜콜린에 대해 HPLC/ELSD를 이용한 신속, 정확한 분석법을 확립하였다. HPLC의 조건은 silica column을 사용하고 헥산:이소프로판올:물-30:60:8을 유속 0.5 ml/min으로 포스파티딜콜린을 분리하였고 ELSD 검출기를 사용하여 nebulizer온도는 $60^{\circ}C$. drift tube온도는 $75^{\circ}C$, carrier gas 30 psi로 하여 20 min내에 분리하였으며 검량선은 250 ppm$\∼$1,000 ppm으로 하여 정량하였다. 시료의 회수율은 $80\%$이상으로 높게 나타났으며 정량한계는 0.15 ppm이였다. 또한 이 분석법을 이용하여 실제 유통되고 있는 레시틴제품(난황레시틴 2종, 대두레시틴8종)에 대해 인지질의 함량과 포스파티딜함량을 조사한 결과, 인지질의 함량은 각각 81.5$\∼$83.3$\%$, $66.0\∼88.0\%$로 나타났으며 포스파티딜콜린의 함량은 각각 74.8$\∼$ 8.6$\%$및 $18.7\∼62.3\%$로 난황레시틴의 함량이 높게 나타났다.

• 설비공학논문집
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• 제29권12호
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• pp.638-644
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• 2017

Optimal cooling operation algorithm was developed based on a simulation case of a single family house model equipped with renewable energy facility. EnergyPlus simulation results were used as virtual test data. The model contained three energy storage elements: thermal heat capacity of the living room, chilled water storage tank, and battery. Their charging and discharging schedules were optimized so that daily electricity bill became minimal. As an optimization tool, linear programming was considered because it was possible to obtain results in real time. For its adoption, EnergyPlus-based house model had to be linearly approximated. Results of this study revealed that dynamic cooling load of the living room could be approximated by a linear RC model. Scheduling based on the linear programming was then compared to that by a nonlinear optimization algorithm which was made using GenOpt developed by a national lab in USA. They showed quite similar performances. Therefore, linear programming can be a practical solution to optimal operation scheduling if linear dynamic models are tuned to simulate their real equivalents with reasonable accuracy.

• 한국멀티미디어학회논문지
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• 제13권2호
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• pp.179-184
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• 2010

인간의 보다 나은 삶을 위한 유비쿼터스 사회에서 센서 네트워크기술과 함께 중요시 되고 있는 기술이 자동제어 및 모션제어기술이다. 산업현장 및 생활환경에서 GUI 환경의 보다 빠르고 정밀한 모션제어 및 위치제어기술에 대한 요구는 날로 늘어가고 있다. 특히, GUI 환경의 개선과 산업현장의 변화에 빠르게 적응할 수 있도록 PC 기반의 모션 제어시스템에 대한 수요가 날로 증가하고 있다. 본 연구에서는 높은 신뢰성과 정밀한 모션제어를 제공하는 NI PXI-7352 컨트롤러와 스텝 모터를 활용하여 사용자인터페이스를 획기적으로 증대시킨 그래픽기반 시스템 프로그래밍언어인 LabVIEW를 기반으로 산업현장에서 다양하게 응용할 수 있는 고성능 2축 스텝 모션 제어시스템을 개발하고자 한다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2007년도 Proceedings of ISRS 2007
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• pp.98-100
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• 2007

Tropical forests have variety of biodiversity values, which provide invaluable services to the living being on earth. In the recent years, tropical forests are regarded as valuable global resources that act as sink for carbon dioxide in order to mitigate global climatic change. In many parts of the world, tropical forests are being rapidly cleared by various means. Soil organic carbon (SOC) is concentrated in the upper 12 inches of the soil. So it is readily depleted owing to the degradation activities. In the present study, it was aimed to assess the magnitude of disturbance in the availability of SOC in a semi- evergreen forest, situated in the Eastern Ghats of Tamil Nadu, India. The forest density of this region was mapped with QuickBird satellite data. Intensive field soil sampling and floristic study were conducted to estimate the SOC status in different density classes and to identify the species availability. The SOC density ranged from 274.06 t/ha to 147.84 t/ha in the very dense and degraded semi-evergreen forest respectively. The SOC content was also varied from 3.70 to 1.83 % in the very dense semi-evergreen and medium semi-evergreen forests respectively. The species composition in different density classes was also varied considerably. As a result of this study, it was identified that the disturbance to forests by various means not only affect the density of forests but also affect the below ground SOC status proportionately.

• KIEAE Journal
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• 제14권3호
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• pp.87-95
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• 2014

The purpose of this study is to evaluate the correlation coefficients between the airtightness and sound insulation performance of Han-style windows in New Han-ok. To achieve these goals, field measurements were accomplished in 18 bedrooms of 16 Han-oks in which actual residents were living, and then lab measurements were proceeded in the reverberation lab for evaluating the sound insulation performance. Followings are results. The results of the correlation analysis between the airtightness(Air change per hour at 50 Pa, ACH50) and sound insulation performance(Sound reduction index, Rw) in bedrooms of actual Han-oks, it was found that there were no significant correlation between two evaluating values. On the other hand, it was analyzed that the correlation coefficients of total 24 structures(double casement windows, single casement window, casement and sliding windows, single sliding window, 6 types per each structure) were located on 0.6757 exponentially and 0.4154 lineary in the lab evaluating conditions. But, The results of evaluating 4 structure classificatorily, it was found that there were high correlation coefficients(0.8665~0.9273 at ACH50, 0.8414~0.9346 at Rw). These results were signified that the correlation coefficients were changed according to the each structure and case by case analysis were necessary at the same time.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 제37회 하계학술대회 논문집 C
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• pp.1627-1628
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• 2006

In this paper, mesoporous only platinum electrode and micro pore platinum electrode with mesoporous Pt are fabricated and characterized on a silicon substrate to check their usability as enzymeless sensing electrodes for developing non-disposable glucose sensors integrated with silicon CMOS read out circuitry. Since most of electrochemical glucose sensors are disposable due to the use of the enzymes that are living creatures, these are limited to use in the in-vivo and continuous monitoring system applications. The proposed mesoporous Pt electrode with approximately 2.5nm in pore diameter and 150nm in height was fabricated by using a nonionic surfactant $C_{16}EO_8$ and an electroplating technique. The micro pore Pt electrode with mesoporous Pt means the mesoporous Pt electrode fabricated on top of micro pore arrayed Pt electrode with approximately $10{\mu}m$ in pore diameter and $80{\mu}m$ in height. The measured current responses at 10mM glucose solution of plane Pt, micro pore Pt, micro pore with mesoporus Pt, and mesoporous Pt electrodes are approximately $9.9nA/mm^2$, $92.4nA/mm^2$, $3320nA/mm^2$ and $44620nA/mm^2$, respectively. These data indicate that the mesoporous Pt electrode is much more sensitive than the other Pt electrodes. Thus, it is promising for non-disposable glucose sensor and electrochemical sensor applications.