• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of International Convention of the Pharmaceutical Society of Korea
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• pp.154.3-155
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• 2001

• 동의생리병리학회지
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• 제22권4호
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• pp.766-770
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• 2008

Bone is a dynamic tissue that is regulated by the balance between bone-resorbing osteoclasts and bone-forming osteoblasts. Curcumin isolated from Kang-hwang (Turmeric) is widely used as a foodstuff, cosmetic, and medicine. However, the effect of curcumin isolated from Kang-hwang in osteoclast differentiation remains unknown. In this study, we sought to examine the role of curcumin in osteoclast differentiation. Here we show that curcumin greatly inhibited RANKL-mediated osteoclast differentiation in osteoclast precursors without cytotoxicity. RANKL induced the phosphorylation of p38 and JNK mitogen-activated protein kinase (MAPK) and mediated $I-{\kappa}B$ degradation in bone marrow macrophages (BMMs). However, RANKL-mediated p38 MAPK phosphorylation was inhibited by the addition of curcumin. Curcumin inhibited the mRNA expression of TRAP, c-Fos, and NFATc1 in BMMs treated with RANKL. Furthermore, the protein expression of c-Fos and NFATc1 induced by RANKL was suppressed by curcumin treatment. Taken together, our results suggest that curcumin may have a potential therapeutic role in bone-related diseases such as osteoporosis by inhibiting osteoclast differentiation.

• 대한본초학회지
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• 제35권4호
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• pp.9-16
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• 2020

Objective : To identify the effects of the water extract of Liriope platyphylla tuber (Liriopis tuber, LT) on the activation of astocytes, we investigated the regulatory effects of LT extract on H₂O₂-induced oxidative damage in C6 rat astrocytes. Methods : LT extract was extracted with boiling water. C6 cell line were treated with LT extract at 1, 2, and 3 mg/㎖ or without for 30 min and then stimulated with H₂O₂ at 5 ㎛ for 24 hr. The cell viability was measured by MTT assay. The expression of glial fibrillary acidic protein (GFAP), signal transducer and activator of transcription 3 (STAT3), phospho-STAT3 (pSTAT3), cyclooxygenase (COX-2), Nuclear factor-κB (NF-κB), superoxide dismutase 2 (SOD2), heme oxygenase-1 (HO-1), catalase, Akt, phospho-Akt (p-Akt) phosphoinositide 3-kinases (PI3K), and protein kinase C alpha (PKCα) proteins were determined by Western blot, respectively. GFAP expression was also observed with immunocytochemistry under a fluorescence microscope. Results : LT extract induced cell proliferation in H₂O₂-stimulated C6 cells. LT extract significantly inhibited the expression of GFAP, NF-κB and COX-2 and increased the expression of HO-1 and the phosphorylation of STAT3 in H₂O₂-stimulated C6 cells. LT extract also significantly increased the phosphorylation of Akt and decreased the expression of PKCα in a dose-dependent manner in H₂O₂-stimulated C6 cells. Conclusions : LT extract can regulate H₂O₂-induced activation of astrocytes through inhibiting the expression of NF-κB, COX-2 and regulating Akt / HO-1, STAT3 or PKCα signaling pathway.

• 동아시아식생활학회지
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• 제15권1호
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• pp.20-28
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• 2005

The dietary effect of water extracts of Liriopis tuber(WELT) in the diabetic SD-rats on the level of blood sugar and serum cholesterol was investigated. The experimental plots divided into normal group(N), diabetic control(DC), 5% WELT-group(WELT-I) and 10% WELT-group (WELT-II). Each group was fed for 6 weeks, then continuously fed for 1 more week after streptozotocin injection. The loss of the body weight fed for one week after induction of the diabetes was 2.2～6.3% in the WELT-I and -II groups, but it was 18.6% in the DC-group. There was no significant difference in the feed intakes after diabetes induction between N-group and WELT-group, while it was significantly increased in DC-group. The feed efficiency ratios before diabetes induction were 1.70 in WELT-I group, 1.53～1.59 in the N, DC and WELT-II group, while the ratios after diabetes induction were 0.92 in DC-group, 1.51～1.83 in the N, WELT-I and -II group. While the amounts of water intakes for one week after diabetes induction was 625.4 mL in the DC-group, and it were 364.3～371.1 mL in the WELT-groups showing no significant difference with N-group. The excretion amounts of urine were 431.96 mL in DC-group for one week after diabetes induction, and it was 182～192.84 mL in WELT-groups. The ratios of liver weight against body weight were 2.74% in N-group, 2.93～2.96% in WELT-groups, but it was 4.01% in DC-group. The level of blood glucose in WELT-groups fed for one week after diabetes induction were 136.8～138.6 mg/dL showing no significant difference with N-group, but it was 357.8 mg/dL in DC-group. The level of serum triacylglycerol and serum total cholesterol were 93.8 and 68.7 mg/dL in N-group, 120.1 and 101.6 mg/dL in DC-group, 97.4～100.6 and 60.8～67.7 mg/dL in WELT-groups, respectively, showing no significant difference between N-group and WELT-groups. HDL-cholesterol/total cholesterol ratio were 0.63 in N-group, 0.57～0.67 in WELT -groups, which was significantly higher than that of DC-group(p<0.05). Atherogenic index were 0.58 in N-group, 0.49～0.74 in WELT-groups, but it was 1.32 in DC-group. The above results suggest that the WELT diets may have both preventive and curing effects against the diabetes.

• 한국식품영양과학회지
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• 제46권3호
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• pp.381-388
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• 2017

본 연구에서는 증숙 맥문동을 첨가한 쿠키의 품질 특성 및 관능적 특성을 반응표면분석법을 이용하여 분석하여 저칼로리 쿠키의 개발 가능성을 검토하고자 하였다. 증숙 맥문동 열수추출물, 버터 및 설탕의 함량을 독립변수로 하고 17가지의 쿠키를 제조하여 쿠키의 특성을 분석한 결과 독립변수에 따라서 반죽의 퍼짐성, 쿠키의 당도, 색도, 경도 및 색, 조직감, 단맛, 외관, 전반적인 기호도와 관련된 관능적 특성이 유의적으로 변화하는 것을 알 수 있었다. 이러한 결과를 바탕으로 설탕의 사용은 최소로 하면서 쿠키의 품질 특성 및 관능 특성에 대한 반응변수의 값을 최대로 나타낼 수 있는 배합비를 분석한 결과 당류 저감화를 위한 최적의 배합비는 증숙 맥문동 열수추출물 6.84 g, 버터 47.20 g, 설탕 36.02 g으로 나타났다. 최적화된 배합비를 토대로 증숙 맥문동 열수추출물이 첨가되지 않은 대조군과 품질 특성 및 관능적 특성을 비교한 결과, 당도는 유의적으로 감소하였으나 관능평가 항목의 단맛은 유의적인 차이가 나지 않으면서 나머지의 관능평가 항목은 유의적으로 증가함을 알 수 있었다. 이상의 결과를 토대로 증숙 맥문동의 활용할 경우 당류 저감화를 위한 다양한 제품의 개발에 중요한 기초 자료로 활용될 수 있을 것으로 판단된다.

• 한국식품영양과학회지
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• 제46권2호
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• pp.229-236
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• 2017

본 연구에서는 증숙 맥문동을 첨가한 양갱 제조조건을 반응표면분석법을 이용하여 최적화하여 저칼로리 양갱의 개발 가능성을 검토하고자 하였다. 증숙 맥문동 열수 추출물, 설탕 및 한천의 함량을 독립변수로 하여 16가지의 양갱을 제조하여 pH, 수분 함량, 당도, 색도 및 갈변도 등의 이화학적 품질 특성과 조직감, 관능평가를 수행하였다. 이화학적 품질 특성 중에서 pH 및 갈변도는 증숙 맥문동 열수 추출액, 당도 및 수분 함량은 설탕, 색도는 증숙 맥문동 열수 추출액과 설탕의 양이 품질 특성에 더 많은 영향을 나타냈으며, 조직감에 있어서 씹힘성과 검성은 분말한천의 양에 의존하는 경향을 나타내었다. 또한, 관능평가에 있어서 유의성을 나타낸 외관, 색, 단맛, 단단함, 질감 및 종합적인 기호도의 경우 증숙 맥문동 열수 추출물, 설탕이 증가할수록 기호도가 증가하는 것으로 나타났다. 이러한 결과를 바탕으로 당류의 사용은 최소화하면서 최적의 양갱의 품질 특성 및 기호도를 나타내는 조건은 증숙 맥문동 열수 추출물이 35 mL, 설탕은 55.23 g, 분말한천은 3.39 g으로 최적 첨가량이 예측되었으며, 기본 배합비와 비교한 결과 단맛은 유의적 차이가 나타나지 않고 그 외의 모든 관능평가 항목에서 유의적으로 우수하였다. 따라서 증숙 맥문동을 식품에 활용할 경우 다양한 저칼로리 제품의 개발에 이용이 가능할 것으로 판단된다.

• 한국작물학회지
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• 제48권
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• pp.79-95
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• 2003

To clarify the botanical origins of oriental medicines which have been argued or confused for plant origins, species of original plants were investigated through the textural research for oriental medicines and the comparison of Chinese, Korean, Japanese and North Korean Pharmacopoeia. Twenty oriental medicines were studied; Angelicae Gigantis Radix, Rhei Rhizoma, Atractylodis Rhizoma Alba, Aconiti Lateralis Radix Preparata, Acanthopanacis Cortex, Osterici Radix, Cnidii Rhizoma, Saposhnikovae Radix, Magnoliae Cortex, Paeoniae Radix, Liriopis Tuber, Zanthoxyli Fructus, Achyranthis Radix, Sinomeni Caulis et Rhizoma, Polygonati Rhizoma, Cinnamomi Cortex, Visci Herba et Loranthi Ramulus, Fritillariae Thunbergii Bulbus, Pogostemonis Herba, and Curcumae Longae Radix.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.511-511
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• 2003

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.234.1-234.1
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• 2003

Spicatoside A and spicatoside B were isolated from Liriopis tuber. Spicatoside A has anticancer activity and it is composed of 1 ${\beta}$-hydroxy diosgenin and trisaccharides. Spicatoside B has the preventive effect of diabetes mellitus and also has 1 ${\beta}$-hydroxy derivatives, which is clevaged the ether linkage of F ring of diosgenin. Therefore, selective synthesis of 1 ${\beta}$-hydroxydiosgenin is required. (omitted)

• 대한본초학회지
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• 제31권1호
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• pp.69-75
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• 2016

Objectives : The aim of this study is to investigate the various effects of individual or combined extract of GamiSaengmaeksan (GSS) on cell viability, anti-inflammatory and antioxidant activityMethods : In order to evaluate cytotoxicity, MTT assay was performed. We investigated the levels of proinflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 and interleukin (IL)-1β, and nitric oxide(NO) in LPS-induced RAW 264.7 cells to check the effects on anti-inflammatory activity. The level of NO production in RAW 264.7 cells was measured by using Griess reagent. The levels of cytokines and ROS were measured by Luminex and Flow cytometry, respectively.Results : At concentration of 200 ㎍/㎖ GSS, cytotoxicity was observed in RAW 264.7 cells. However, at concentration less than 100 ㎍/㎖ of both combine and individual GSS, cytotoxicity was not observed in Raw 264.7 cells. However, the level of ROS in RAW 264.7 cells were decreased at both extract of 100 ㎍/㎖ GSS. Also, the level of NO in RAW 264.7 cells were decreased from extraction of concentration of 100 ug/ml in GSS and individual-extraction of Liriopis Tuber, White Ginseng and Glycyrrhizae Radix. In addition, productions of pro-inflammatory cytokines (TNF-α) in LPS-induced RAW 264.7 cells were decreased from extraction of concentration of 10 and 100 (㎍/㎖) in GSS and individual-extraction of Liriopis Tuber.Conclusions : It is concluded that combined extract of GSS appears to be more effective in anti-oxidation and anti-inflammatory effect than those in individual-extraction of GSS. These results may be developed as a raw material for new therapeutics to ease the symptoms related with inflammatory and oxidative stress.