• Ocean Systems Engineering
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• v.1 no.1
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• pp.31-57
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• 2011

Impact pressure due to sloshing is of great concern for the ship owners, designers and builders of the LNG carriers regarding the safety of LNG containment system and hull structure. Sloshing of LNG in partially filled tank has been an active area of research with numerous experimental and numerical investigations over the past decade. In order to accurately predict the sloshing impact load, a new numerical method was developed for accurate resolution of violent sloshing flow inside a three-dimensional LNG tank including wave breaking, jet formation, gas entrapping and liquid-gas interaction. The sloshing flow inside a membrane-type LNG tank is simulated numerically using the Finite-Analytic Navier-Stokes (FANS) method. The governing equations for two-phase air and water flows are formulated in curvilinear coordinate system and discretized using the finite-analytic method on a non-staggered grid. Simulations were performed for LNG tank in transverse and longitudinal motions including horizontal, vertical, and rotational motions. The predicted impact pressures were compared with the corresponding experimental data. The validation results clearly illustrate the capability of the present two-phase FANS method for accurate prediction of impact pressure in sloshing LNG tank including violent free surface motion, three-dimensional instability and air trapping effects.

• Macromolecular Research
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• v.14 no.1
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• pp.1-33
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• 2006

Polyimides (PIs) exhibit excellent thermal stability, mechanical, dielectric, and chemical resistance properties due to their heterocyclic imide rings and aromatic rings on the backbone. Due to these advantageous properties, PIs have found diverse applications in industry. Most PIs are insoluble because of the nature of the high chemical resistance. Thus, they are generally used as a soluble precursor polymer, which forms complexes with solvent molecules, and then finally converts to the corresponding polyimides via imidization reaction. This complexation with solvent has caused severe difficulty in the characterization of the precursor polymers. However, significant progress has recently been made on the detailed characterization of PI precursors and their imidization reaction. On the other hand, much research effort has been exerted to reduce the dielectric constant of PIs, as demanded in the microelectronics industry, through chemical modifications, as well as to develop high performance, light-emitting PIs and liquid crystal (LC) alignment layer PIs with both rubbing and rubbing-free processibility, which are desired in the flat-panel display industry. This article reviews this recent research progresses in characterizing PIs and their precursors and in developing low dielectric constant, light-emitting, and LC alignment layer PIs.

• Special Issue of the Society of Naval Architects of Korea
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• 2005.06a
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• pp.22-30
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• 2005

Sloshing loads, produced by the violent liquid free-surface motions inside the cargo tank have become an important design parameter in ship building industry since there have been demands for the increased sizes of the cargo containment system of LNG carriers. In this study, sloshing impact pressure acting on the shell of the spherical cargo tank of an LNG carrier as well as dynamic pressure and flow behavior around the pump tower located at the center of the tank have been calculated. Comparative numerical sloshing simulations for a spherical LNG tank using 2-D LR.FLUIDS which is based on the finite difference method and 3-D MSC.DYTRAN which is capable of calculating nonlinear fluid-structure interaction have been carried out. A method of calculating sloshing-induced dynamic loads and the subsequent structural strength analysis for pump tower of a spherical LNG carrier using MSC. DYTRAN and MSC.NASTRAN have been presented.

• BMB Reports
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• v.37 no.4
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• pp.460-465
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• 2004

A 16-residue polypeptide model with the sequence acetyl-YALSLAATLLKEAASL-OH was derived by rational de novo peptide design. The designed sequence consists of amino acid residues with high propensity to adopt an alpha helical conformation, and sequential order was arranged to produce an amphipathic surface. The designed sequence was chemically synthesized using a solid-phase method and the polypeptide was purified by reverse-phase liquid chromatography. Molecular mass analysis by electro-spray ionization mass spectroscopy confirmed the correct designed sequence. Structural characterization by circular dichroism spectroscopy demonstrated that the peptide adopts the expected alpha helical conformation in 50% acetonitrile solution. Liposome binding assay using Small Unilamellar Vesicle (SUV) showed a marked release of entrapped glucose by interaction between the lipid membrane and the tested peptide. The channel-forming activity of the peptide was revealed by a planar lipid bilayer experiment. An analysis of the conducting current at various applied potentials suggested that the peptide forms a cationic ion channel with an intrinsic conductance of 188 pS. These results demonstrate that a simple rational de novo design can be successfully employed to create short peptides with desired structures and functions.

• Journal of Aerospace System Engineering
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• v.12 no.2
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• pp.15-22
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• 2018

A compressible multiphase flow was investigated using a DIM consisting of seven equations, including the fifth-order MLP and a modified HLLC Riemann solver to achieve a precise interface structure of liquid and gas. The numerical methods were verified by comparing the flow structures of the high-pressure water and low-pressure air in the shock tube. A 2D air-helium shock-bubble interaction at the incident shock wave condition (Mach number 1.22) was numerically solved and verified using the experimental results.

• Journal of the Earthquake Engineering Society of Korea
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• v.3 no.4
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• pp.71-82
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• 1999

An axisymmetric shell element which includes the effects of the meridional and circumferential cable prestresses is developed. The fluid-structure interaction is expressed as added mass effect which is in proportion to the acceleration of the structure in interface surface. The added mass is obtained by using finite element method under the assumption that the fluid is invicid, incompressible and irrotational. It is coded for personal computer by the maximum use of axisymmetic properties and the dynamic analysis are performed under seismic exitations. A ring element makes the characteristics of the axisymmetric shell to be fully utilized. The elgenvalue solutons under the initial prestresses and the internal fluid are well agreed with the exact solutions and references by using under 20 elements. The eigenvalues are decreased along the increasing the height of internal fluid and these effects are dominant under the lower wave numbers. The results of the seismic analysis show that the radial deflection under the meridional prestress is a little larger than that under the circumferential prestress.

• Computational Structural Engineering
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• v.10 no.4
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• pp.291-302
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• 1997

This paper presents an analytical method for evaluating the free vibration of a circular cylindrical tank filled with bounded compressible fluid. The analytical method was developed by means of the finite Fourier series expansion method. The compressible fluid motion was determined by means of the linear velocity potential theory. To clarify the validity of the analytical method, the natural frequencies of a circular cylindrical tank with the clamped-clamped boundary condition, and filled with water, were obtained by the analytical method and the finite element method using a comercial ANSYS 5.2 software. Excellent agreement on the natural frequencies of the liquid-filled tank structure was found. The compressiblity and the fluid density effects on the normalized coupled natural frequencies were investigated. The density of fluid affects on all coupled natural frequencies of the tank, whereas the compressibility of fluid affects mainly on the natural frequencies of lower circumferential modes.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.395-402
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• 2005

Biologically active recombinant human follicle stimulating hormone (rhFSH) was produced in Chinese hamster ovary cells and purified by a series of chromatographic steps. The chromatographic steps included anion-exchange chromatography (DEAE Sepharose F/F, Q Sepharose F/F), hydrophobic interaction chromatography (Source 15 PHE), and hydroxyapatite chromatography (Macro-Prep ceramic hydroxyapatite type I). A distinctive step of the purification process developed was the use of ZnCl$_2$ for the removal of non-glycosylated or lowly-glycosylated FSH and impurities through co-precipitation with Zn$^{2+}$. Purified rhFSH was identified and characterized by several physicochemical and biological methods such as gel electrophoresis, high-performance liquid chromatography, amino acid analysis, carbohydrate analysis, and biological activity. The overall yield of the purification was ~$30\%$. The rhFSH preparation obtained showed high purity (>$99\%$) and high in vivo potency (>16,000 IU/mg). Carbohydrate analysis suggested that the purified rhFSH contained approximately $40\%$ (w/w) carbohydrate with di­or tri-antennary structure on average, which is somewhat more heavily sialylated than commercially available rhFSH. In conclusion, the results of these analyses established an identity of the purified rhFSH with natural FSH from human pituitary glands, and furthermore, the purified rhFSH preparation showed higher in vivo potency and was slightly more heavily sialylated than commercially available rhFSH.

• Archives of Pharmacal Research
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• v.24 no.2
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• pp.144-149
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• 2001

N-acetylsphingosine (C2-ceramide) is a synthetic water-soluble ceramide mimicking the activity of natural ceramides. By fixing chiral conformation on carbon numbers 2 and 3 in the ceramide structure, four chiral C2-ceramides naming d-erythro-, I-erythro-, d-threo-and 1-three C2-ceramide were synthesized. We have investigated the chiral effects of these C2-ceramides on the sphingolipid metabolism, particularly on both the sphingolipid bio- synthetic pathway and on the degradation pathway. In both HL-60 and U937 cells, the chiral C2-ceramide ($10{\mu}\textrm{m}$) showed sphingosine accumulation monitored fluoromatrically by a high performance liquid chromatographic separation of the sphingoid bases. Most importantly, in HL-60 cells, l-erythro C2-ceramide induced a 50 fold increase in sphingosine as compared to the control, while l-threo C2-ceramide exhibited a minimal 7-fold in-crease. In contrast, sphinganine, another sphingoid base, showed less accumulation by any chiral C2-ceramide tested under the same conditions. These results suggested that chiral C2-ceramide primarilyacts on the sphingolipid degradation pathway rather than on the sphingolipid biosynthetic route. The strong $C_0/G_1$ phase arrest in the cell cycle by treatment of I-erythro C2-ceramide indicates that the blockade of the sphingolipid degradation pathway might be concomitantly involved in the dysfunction of the cell cycle. On the other hand, the fact that all chiral C2-ceramides tested failed to inhibit the activity of sphingosine kinase acting on the removal of sphingosine by producing sphingosine-1 -phosphate demonstrates that chiral C2- ceramides may increase sphingosine by activating various ceramidases by which natural ceramides are divided into sphingosine and free fatty acids. However, the precise steps involved in this interaction are still unknown.

• Analytical Science and Technology
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• v.6 no.1
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• pp.107-119
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• 1993

The purpose of this study is to investigated the elution behavior of platinoid metal acetylacetonates, which is the key to elucidate their retention mechanism and optimize their RPLC separation conditions. The retention data of four platinoid metal acetylacetonates have been measured on four different columns in methanol-water and acetonitrile-water systems. The retention of uncharged platinoid metal acetylacetonates is interpreted by solvophobic effect. The retention of platinoid metal acetylacetonates is also greatly influenced by the geometric structure of the complexes. The square planar chelates, $Pd(acac)_2$, $Pt(acac)_2$, are retained longer than the octahedral chelates, $Rh(acac)_3$, $Ir(acac)_3$. It is likely due to that square planar chelates show greater interaction with nonpolar stationary phase than octahedral chelates. The results of van't Hoff plots have shown that platinoid metal acetylacetonates is operated on the same retention mechanism in the temperature range of $25{\sim}45^{\circ}C$. The study of the retention mechanism by the enthalpy-entropy compensation phenomenon has indicated that the retention mechanism of octahedral chelates and square planar chelates do not vary with the composition change of methanol-water mobile phase, respectively. In acetonitrile-water mobile phase, however, the retention mechanism is observed to be more complicated. Optimum condition for the separation of four platinoid metal acetylacetonates is found to be 40% methanol, polymeric C18 column, and $45^{\circ}C$.