• 한국식품저장유통학회지
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• 제5권2호
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• pp.127-132
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• 1998

In order to fad out an efficient way of topdressing nitrogen fertilizer in mulch-cropping system of onion(Allium cepa L.), solid, slow-release, and liquid forms of nitrogen fertilizers were allied to cv. 'Changnyungdaego' various number of times at different time, with 5 topdress applications of solid fertilizer serving as a control. Whole basal application of conventional solid fertilizer and 2 slow-release fertilizers were labor-saving and showed improved storage quality of bulbs, but resulted in poor plant growth and considerably low yield due to fertilizer shortage from early April. This suggests that topdress application is necessary. Liquid form of nitrogen fertilizer was more effective for plant growth and yield and saving labor than the solid form. Early applications was effective for increasing yield and storage quality of onion bulbs harvested. Thus two applications of liquid form of nitrogen fertilizer in February and March at rome month interval are recommended in mulch crowing system of onion.

• 한국잠사곤충학회지
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• 제42권1호
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• pp.1-5
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• 2000

To investigate the possibility of crypreservation of mulberry seeds in liquid nitrogen(LN$_2$), characteristics of the seeds were examined after picking mulberry syncarps and drying-heat treatment. Storage in LN$_2$has the potential of providing indifinite preservation of valuable seed germplasm. Determining the tolerance of seeds among given cultivars to LN$_2$cooling and subsequent rewarming is the first step to establishing the feasibility of LN$_2$storage. Seeds of 4 mulberry varities were treated to LN$_2$(-196$\^{C}$) for 24 hours after drying heat treatment. Seed moisture content of Daeryukppong was the highest. As moisture content of mulberry seed was below 1%, storage in LN$_2$was safe. And drying heat treatment for 60 minutes was suitable to prevent decreased germination rate and germination vigor of seeds. The seeds of Cheongilppong were unsuibable to cryopreserve in LN$_2$for longterm storage.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2008년도 하계학술발표대회 논문집
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• pp.142-147
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• 2008

This paper reports an analysis of self-pressurization in a closed cryogenic liquid storage system and its comparison with experimental data using liquid nitrogen. Partial equilibrium model(PEM), revised thermodynamic analysis of homogeneous model, has been applied for the pressurization in a closed tank. The vapor and liquid bulk temperature and the liquid-vapor interface temperature are separately calculated as their own representative values in this analysis. The analysis results of the partial equilibrium model are compared with the experimental data and other preceding homogeneous temperature models for validation.

• 대한수의학회지
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• 제33권2호
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• pp.285-287
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• 1993

Toxoplasma gondii frozen by the method of very slow cooling ($-30^{\circ}C/3hrs.{\rightarrow}\;-60^{\circ}C/lhr.{\rightarrow}\;-196^{\circ}C$ of liquid nitrogen)were inoculated mice after storage in the liquid nitrogen for 5 years. The results obtained were as follows; 1. Toxoplasma gondii was detected in mice 4~5th day after intraperitoneal inoculation of organisms stored in the liquid nitrogen once a year for 5 years. 2. Toxoplasma gondii could be preserved longer than 5 years in liquid nitorgen being evidenced by the in vivo test.

• 한국터널지하공간학회 논문집
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• 제18권2호
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• pp.175-181
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• 2016

본 연구는 액체질소와 같이 급속 동결이 가능하면서 질식의 위험을 배제할 수 있는 냉매로서 액화공기를 선정하였고 이의 적용성을 평가하였다. 액화공기의 안정성을 평가하기 위해 액체질소와 액체산소가 혼합된 액화공기의 산소 농도가 산업안전보건법에 제시된 산소 농도 기준에 부합되는지를 실험적으로 검증하였으며, 액체질소 및 액체산소의 혼합 비율, 액화공기 저장용기의 압력변화 및 유량변화에 따른 액화공기 산소 농도변화를 살펴보았다. 그 결과, 액체질소 및 액체산소를 8:2로 혼합하였을 경우 산업안전보건법에 제시된 산소 농도 기준에 부합되는 것을 확인하였다. 액화공기 저장용기의 압력변화 및 유량변화는 액화공기의 산소 농도에 큰 영향을 미치지 않는 것으로 나타났다.

• 한국수소및신에너지학회논문집
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• 제26권3호
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• pp.234-240
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• 2015

In the face of the world's growing energy storage needs, liquid hydrogen offers a high energy density solution for the storage and transport of energy throughout society. A 5 L liquid hydrogen storage tank has been designed, fabricated and tested to investigate boil-off rate of liquid hydrogen. As the insulation plays a key role on the cryogenic vessels, various insulation methods have been employed. To reduce heat conduction loss, the epoxy resin-based insulation supports G-10 were used. To minimize radiation heat loss, vapor cooled radiation shield, multi-layer insulation, and high vacuum were adopted. Mass flow meter was used to measure boil-off rate of the 5 L cryogenic vessel. A series of performance tests were done for liquid nitrogen and liquid hydrogen to compare with design parameters, resulting in the boil-off rate of 1.7%/day for liquid nitrogen and 16.8%/day for liquid hydrogen at maximum.

• 한국균학회지
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• 제34권1호
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• pp.54-58
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• 2006

액체질소 보존법은 동결 건조 보존이 불가능한 것을 포함한 곰팡이를 가장 효과적으로 보존할 수 있는 방법 중의 하나로서, 유전적인 변화와 오염을 방지하고 장기보존이 가능한 이점을 가진다. 크료튜브 대신 액체질소 보존에 사용할 수 있는 폴리프로필렌 스트로는 경제성, 안전성, 편리성 그리고 공간 이용성 등의 이점을 가진다. 이에 한국농업미생물자원센터(KACC)에서는 폴리프로필렌 스트로를 이용한 곰팡이의 액체질소 보존법을 정립하였으며, 이를 상세하게 소개하였다.

• 한국정밀공학회지
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• 제10권2호
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• pp.138-145
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• 1993

The bath cryostat of cryogenic apparatuses which are generally used to study physical phenomena under low temperature and ultra low temperature has been desigened and constructed. The practical use of the cryostat is verified by the measurement of the storage life of liquid heloum and liquid nitrogen vessels. The cryostat consists of triple structure of high vacuum environment in order to minimize the evaporation rate of liquid helium and liquid nitrogen by thermal conductivity and radiant heat. The minimum thickness which can stand against inner and outer pressures is calculated from considering the strength of the material.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2009년도 추계학술발표대회 논문집
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• pp.409-414
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• 2009

Since cryogens are stored at very low temperatures, the cryogenic storage systems are quite sensitive to heat leaks. Even though the vessel operated under sealed condition with vacuum insulation and reflective coatings are used, the heat leakage into the vessel is still unavoidable. Therefore, this paper concerns with numerical study of self-pressurization used to analysis the optimum design with the variation volume fraction, effect of heat flux and storage pressure of liquid nitrogen. The result shows that as the volume fraction increases, the pressure rise reduces and the relatively at atmosphere pressure is better than the higher one. In addition, higher heat flux leads the pressure rise increases faster than low one. The additional of heat pipe system to reduce the pressure rise rate also has been done. By this comparison, the optimum design for storage umbilical cord blood can be selected.

• Design & Manufacturing
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• 제14권2호
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• pp.1-6
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• 2020

Recently, research and commercialization related to the field of cell-based therapeutic drug development has been actively conducted. In order to maintain cell viability and prevent contamination, refrigeration preservation devices, such as CRF (controlled rate freezer) or vapor type LN2 tanks have been developed. On the other hand, the storage container for liquid nitrogen tanks currently on sale minimizes the flow structure to prevent structural defects when stored in a liquid nitrogen tank having a high thermal conductivity than vapor nitrogen. If the cell-based treatment drug is stored in the gaseous LN2 tank as it is, the cell survival after thawing is greatly reduced. It was estimated that the existing storage container structure was a factor that prevented the rapid entry and circulation of gaseous nitrogen into the container. Therefore, this study intends to propose a new supercellular storage container model that can maintain the mechanical strength while maximizing the fluid flow structure. To this end, we estimated that the structural change of the storage container effects on the equivalent stress formed around the through-holes of them when exposed to a cryogenic environment using thermal-structural coupled field analysis. As a result of storage experiments in the gas phase tank of the cell-based therapeutic agent using the developed storage container, it was confirmed that the cell growth rate was improved from 66% to 77%, which satisfied the transportation standards of the FDA(Food and Drug Administration) cell-based therapeutic agent.