• Title/Summary/Keyword: Liquid nitrogen

Search Result 1,380, Processing Time 0.03 seconds

Changes of Nutrients in Media and Mycelia on Liquid Spawn Culture of Lentinula edodes (표고 액체종균 배양시 배지와 균사체의 양분변화)

  • Shim, Kyu-Kwang;Yoo, Young-Jin;Koo, Chang-Duck;Kim, Myung-Kon
    • The Korean Journal of Mycology
    • /
    • v.42 no.2
    • /
    • pp.144-149
    • /
    • 2014
  • Lentinula edodes liquid spawn growth under explosive aeration (supplying air with tiny bubbles) and soybean meal addition to liquid culture medium were investigated in terms of mycelial growth and residual free sugar content. The two treatments were effective for homogeneous culturing of mycelial spawn and for separating colonies during multiplication after an exponential growth period without limiting sustaining nitrogen nutrients. The mycelial growth and carbon dioxide concentration were greatest on the 13th day since the inoculation. At 12th day, however, free sugars were almost depleted in the upper part of the liquid medium. Total nitrogen content within precipitated mycelia was the highest at the 13th day. Chitin and sucrose contents in the mycelia were the highest at the 18th day, but ergosterol content became highest at 22 days. These results suggest that Lentinula edodes liquid spawn is ready in 18 days after inoculation.

Chemical properties of liquid swine manure for fermentation step in public livestock recycling center

  • Lee, Dong Sung;Lee, Jae-Bong;Lee, Myoung-Yun;Joo, Ri-Na;Lee, Kyo-Suk;Min, Se-Won;Hong, Byeong-deok;Chung, Doug-Young
    • Korean Journal of Agricultural Science
    • /
    • v.43 no.3
    • /
    • pp.424-431
    • /
    • 2016
  • The nutrients in livestock manure produced during fermentation processes in public livestock recycling centers are used as fertilizers. However, the large amounts of swine manure produced in intensive livestock farms can be a nonpoint source of pollution. In this experiment, we investigated the chemical properties, inorganic components, and heavy metal contents in 101 samples of liquid swine manure collected from 28 public livestock recycling centers throughout the nation. Results showed that the average pH of the samples was alkaline (pH range 5.18 to 9.54), and their maximum EC was $53.2dS\;m^{-1}$. The amounts of total nitrogen and total phosphorus were in the range of 1000 - 2000 and $200-800mg\;L^{-1}$ while potassium, which constituted 47% of the total inorganic ions recovered from the liquid swine manure, amounted to $1500mg\;L^{-1}$. The most distinctive heavy metals recovered from the liquid swine manure were copper and zinc although the amounts of both heavy metals were much lesser than those of the standards as livestock liquid fertilizer set by the Rural Development Administration. On the other hand, the amount of nitrogen decreased rapidly with an increasing fermentation period from immature to mature, assumed to be lost as volatile compounds, such as ammonia, which are the major odor components during the fermentation process.

RETF 액체산소 공급설비 및 엔진 수류시험

  • Han, Yeoung-Min;Cho, Nam-Kyung;Kim, Seung-Han;Chung, Yong-Ghap;Park, Sung-Jin;Lee, Kwang-Jin;Kim, Young-Han;Moon, Il-Yoon
    • Aerospace Engineering and Technology
    • /
    • v.1 no.2
    • /
    • pp.123-131
    • /
    • 2002
  • In this paper, characteristics of cryogenic liquid oxygen was examined during cold flow of KSR-III main engine at each stage. The effect of venting was examined at the stage of cooling and at the pressurization stage, the interaction between nitrogen gas and liquid oxygen was also examined. The characteristic of liquid oxygen in the engine manifold was analyzed. The results showed that venting was the primary role at the cooling process and the interaction of nitrogen gas and liquid oxygen in the run tank is limited at the surface area. With the sampling rate of 1KHz static and dynamic pressure were measured in the rocket engine manifold and in the LOX supply equipment. 32.5mm and 38mm orifice were installed for the tests and pressure condition of liquid oxygen was 23Bar, 29Bar, 41Bar. Increase of orifice diameter and decrease of supply pressure reduced the perturbation of pressure in engine manifold.

  • PDF

Exergy Analysis and Evaluation of Cryogenic Nitrogen Production Process (초저온 질소생상공정의 Exergy 해석 및 평가)

  • 용평순;뭉홍만;이성철
    • Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
    • /
    • 1999.02a
    • /
    • pp.169-172
    • /
    • 1999
  • The exergy method is used for analysis and evaluation of the cryogenic nitrogen production process which is operated by expansion turbene and liquid nitrogen. The exergy loss and thermodynamic effeciency are calculated for the each process. Also the operating efficiency and the exergy distribution are examined for each unit of proces. The optimal conditions to minimize the exergy loss of nitrogen column are found that nitrogen recovery ratio is maximum and operating pressure is 5.0 kg/cm2g. The exergy method can be used to design a plant and to evaluate its process.

  • PDF

Submerged Culture of Phanerochaete chrysosporium and Lignin Peroxidase Production (Phanerochaete chrysosporium의 액체 배양 및 Lignin Peroxidase 생산)

  • Park, Se-Keun;Jeong, Myoung-Sun;Kim, Yeong-Kwan
    • Journal of Industrial Technology
    • /
    • v.21 no.A
    • /
    • pp.343-349
    • /
    • 2001
  • This study characterizes the growth of white rot fungi Phanerochaete chrysosporium IFO 31249) and lignin peroxidase(LiP) activity in different submerged culture media. P. chrysosporium was grown in the form of pellet of various sizes from a spore inoculum under shaking liquid culture condition. While the growth of mycelia was higher under the nitrogen-sufficient culture than under the nitrogen-limited culture, ligninase activity was relatively lower. The lignin peroxidase appeared in nitrogen-limited culture and was suppressed by excess nitrogen. High level(40U/l) of lignin peroxidase activity was obtained in the growth medium containing 1.5mM veratryl alcohol, a secondary metabolite of P. chrysosporium. Lignin peroxidase production was not observed under conditions of nitrogen sufficiency or in balanced media, suggesting that control parameters could increase the activity by manipulating the secondary metabolism.

  • PDF

Methodology for Describing Different Phase States of Molecular Nitrogen

  • Cho, Haeng Muk;Kudryavtsev, I.N.;Kramskoy, A.V.
    • Journal of Energy Engineering
    • /
    • v.23 no.4
    • /
    • pp.215-222
    • /
    • 2014
  • A theory-based methodology for describing the thermodynamic properties of molecular nitrogen is presented. The results obtained indicate a successful application of a fully consistent statistical method for the description of a molecular system in different phase states. The method employs a density of states equation for solid nitrogen and a perturbation potential for gaseous and liquid nitrogen. The main characteristics of the calculation method include the need for a minimal number of initial data and the absence of fitting parameters. The adequacy of the physical model that is the basis for the method allows a description of existing experimental data and the peculiarities of the thermodynamic properties.

Measurement of Nitrogen Solubility with Ti addition in Stainless Steel (스테인레스강중의 Ti첨가에 따른 질소용해도 측정)

  • Lee, Young-Uk
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.10 no.11
    • /
    • pp.3043-3047
    • /
    • 2009
  • Nitrogen solubility and nitride formation in liquid 304 Stainless steel has been measured by sampling method at various temperatures. Also values of thermodynamic functions are determined. Nitrogen solubility in molten 304 Stainless steel is increased with temperature and under the 1atm nitrogen atmosphere, titanium nitride is formed over 0.03wt%Ti in 304Stainless steel. Produced nitride is identified TiN by EDS analysis.

REBCO coil operation in gaseous helium and solid nitrogen

  • Diev, D.N.;Makarenko, M.N.;Naumov, A.V.;Polyakov, A.V.;Shcherbakov, V.I.;Shutova, D.I.;Surin, M.I.
    • Progress in Superconductivity and Cryogenics
    • /
    • v.21 no.3
    • /
    • pp.47-50
    • /
    • 2019
  • The paper gives the results of the experiments with a model two-section REBCO solenoid cooled by either gaseous helium (GHe) or sub-cooled/solid nitrogen (SN2) in (50-77) K temperature range. The major cooling source was a single-stage cryocooler Sumitomo CH-110 with the cooling power of 175 W and 130 W at 77 K and 50 K respectively. The coil itself was not directly conduction cooled. We compare the time taken by both coolants to obtain the temperature of the magnet of about 50 K and the homogeneity of the temperature distribution within the cryostat. Test results for the coil operation in solid nitrogen together with the comparison of its critical properties in SN2 and GHe are also presented.

Comparison of Physico-Chemical Properties of Organic Liquid Fertilizer Made from Seaweed by Adding Microorganism and Molasses (해초류를 이용한 유기 액비 제조 시 발효 미생물원 및 당밀 첨가에 따른 액비의 특성 비교)

  • An, Nan-Hee;Cho, Jung-Rai;Shin, Jae-Hun;Ok, Jung-Hun;Kim, Seok-Cheol
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.23 no.4
    • /
    • pp.32-39
    • /
    • 2015
  • Objective of this study was to investigate characteristics of inorganic components contained in liquid fertilizer produced using seaweed by adding microorganisms and molasses. Addition of dry yeast to liquid fertilizer resulted in little change in pH and considerable increase in EC with high EC value compared to other liquid fertilizers which have microorganisms additives. Also, it was appeared that the dry yeast-added treatment had higher $NH_4-N$ concentration than other treatments. In the other hand, addition of molasses resulted in low pH compared to the control which has no additives, and EC was not different depending on the amount of molasses. $NH_4-N$ concentration in the 2% molasses added treatment was lowest and it showed a significant difference in the no and 1% molasses added treatments. In conclusion, it was shown that addition of dry yeast to liquid fertilizer increased ammonium nitrogen concentration by accelerating nitrogen mineralization, while molasses has an effect of inhibiting nitrogen mineralization. With application of organic liquid fertilizer containing seaweed increased the fresh weight of chinese cabbage.

Effect of SCODMn and pH Adjustment on Physicochemical Characteristics in Liquid Fertilizer Production Process Using Swine Manure (SCODMn 농도 및 pH 조정이 양돈분뇨의 후숙발효과정에 미치는 이화학적 영향)

  • Hong, In-Gi;Kim, Soo-Ryang;Lee, Myung-Gyu
    • Journal of Animal Environmental Science
    • /
    • v.18 no.sup
    • /
    • pp.13-20
    • /
    • 2012
  • This research investigated the effect of $SCOD_{Mn}$ concentrations and pH adjustment at the stage before land application, namely 2nd-aeration treatment stage of liquid fertilizer in the liquid fertilizer treatment process of swine manure on the physicochemical compositions of 2nd-aeration treated liquid fertilizer. The liquid fertilizer used in this research is the alkaline fermented liquid fertilizer of swine manure more than pH 9.0 through aeration treatment (Alkaline fermentation treatment group). About the alkaline liquid fertilizer, phosphate neutralization treatment was conducted with phosphoric acid and it was a phosphate neutralization treatment group. In 2nd-aeration treatment of liquid fertilizer for 30 days, each group was divided into alkaline treatment groups (T-1, T-2, and T-3) and phosphate neutralization treatment groups (T-4, T-5, and T-6) according to early $SCOD_{Mn}$ concentrations. The research results are as follows. 1. As for $SCOD_{Mn}$ reduction rate, the average 29.9% in alkaline treatment groups and the average 36.9% in phosphate neutralization treatment groups were shown and so the relatively high reduction rate was shown in phosphate neutralization treatment groups. 2. After finishing the experiment, the group of the lowest $SCOD_{Mn}$ concentrations was the phosphate neutralization treatment group, T-6 with the lowest inflow concentrations. In case the final goal level of 2nd-aeration treated liquid fertilizer is assumed as concentrations less than $SCOD_{Mn}$ 3,000 ppm, it would be desired that inflow concentrations of 2nd-aeration treatment groups are adjusted less than $SCOD_{Mn}$ 5,500 ppm. 3. As for the persistence rate of nitrogen, the average 29.3% in alkaline treatment groups and the average 38.9% in phosphate neutralization treatment groups were shown and so phosphate neutralization treatment groups showed the relatively low loss rate of nitrogen, meanwhile, in the case of T-P, phosphate neutralization treatment groups maintained high concentrations (average 1,473 ppm). 4. In the event of 2nd-aeration treatment of liquid fertilizer, "alkaline fermentation treatment" condition in 'low phosphate-low nitrogen' type and "phosphate neutralization treatment" condition in 'high phosphate-high nitrogen' type are expected to be favorable.