• Journal of Yeungnam Medical Science
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• 제21권2호
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• pp.215-223
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• 2004

실리콘의 부적절한 피하 주입으로 발생한 폐색전증의 방사선학적 특징은 초기에는 호흡기 증상만 보이지만 염증 매개성 반응이 진행함에 따라 양측 폐 침윤이 급격히 진행하는 것을 볼 수 있었다. 또한 실리콘의 비중이 혈액보다 낮아 중력 비의존성 분포를 보임을 알 수 있었고, 이는 기존의 다른 원인에 의한 폐색전증과 구분되는 소견이다.

• 대한상부위장관⦁헬리코박터학회지
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• 제18권3호
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• pp.168-173
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• 2018

Gastric cancer is still the leading cause of cancer deaths, especially in Asian countries. Recently, many studies have analyzed cell-free nucleic acids (cfNAs) circulating in the blood, for the early diagnosis of cancer and monitoring its progression. Circulating tumor nucleic acids (ctNAs) originate in a tumor and contain tumor-related genetic or epigenetic alterations. This review defines the nomenclatures of each form of cfNAs and describes the characteristics of circulating tumor DNA (ctDNA) and microRNA (miRNA), two major forms of ctNAs studied in gastric cancer research to date. We compare available studies on ctDNA, and explain trends observed in studies of miRNAs in gastric cancers. As these new blood-based biomarkers have attracted increasing attention, we have discussed several important points to be considered before the clinical translation of ctNA detection. We have also discussed the current status of research in this field, and clinical applications of specific ctNAs as tumor markers for gastric cancer diagnosis.

• BMB Reports
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• 제55권11호
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• pp.553-558
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• 2022

Hepatocellular carcinoma (HCC) is dangerous cancer that often evades early detection because it is asymptomatic and an effective detection method is lacking. For people with chronic liver inflammation who are at high risk of developing HCC, a sensitive detection method for HCC is needed. In a meta-analysis of The Cancer Genome Atlas pan-cancer methylation database, we identified a CpG island in the USP44 promoter that is methylated specifically in HCC. We developed methylation-sensitive high-resolution melting (MS-HRM) analysis to measure the methylation levels of the USP promoter in cell-free DNA isolated from patients. Our MS-HRM assay correctly identified 40% of patients with early-stage HCC, whereas the α-fetoprotein test, which is currently used to detect HCC, correctly identified only 25% of early-stage HCC patients. These results demonstrate that USP44 MS-HRM analysis is suitable for HCC surveillance.

• Journal of Digestive Cancer Research
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• 제6권2호
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• pp.59-63
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• 2018

지난 10 년 동안 순환하는 종양 세포는 새로운 바이오마커 및 기초 연구 대상으로 엄청난 주목을 받았다. 최근에는 순환 종양 DNA, 엑소좀 및 마이크로 RNA에 대한 연구도 활발하게 진행되고 있다. 순환 종양 표지자는 게놈 / 면역 프로필 결정, 반응 및 내성 모니터링, 조기 진단 및 예후 예측 이상의 치료제 선택과 같은 정밀 의학의 기본이 될 잠재력을 가지고 있다. 여기에서는 다양한 순환 종양 표지자의 진단 방법, 효능, 의미 및 적용 가능성을 소개하고자 한다.

• BMB Reports
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• 제57권3호
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• pp.155-160
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• 2024

Lung cancer carries one of the highest mortality rates among all cancers. It is often diagnosed at more advanced stages with limited treatment options compared to other malignancies. This study focuses on calnexin as a potential biomarker for diagnosis and treatment of lung cancer. Calnexin, a molecular chaperone integral to N-linked glycoprotein synthesis, has shown some associations with cancer. However, targeted therapeutic or diagnostic methods using calnexin have been proposed. Through 1D-LCMSMS, we identified calnexin as a biomarker for lung cancer and substantiated its expression in human lung cancer cell membranes using Western blotting, flow cytometry, and immunocytochemistry. Anti-calnexin antibodies exhibited complement-dependent cytotoxicity to lung cancer cell lines, resulting in a notable reduction in tumor growth in a subcutaneous xenograft model. Additionally, we verified the feasibility of labeling tumors through in vivo imaging using antibodies against calnexin. Furthermore, exosomal detection of calnexin suggested the potential utility of liquid biopsy for diagnostic purposes. In conclusion, this study establishes calnexin as a promising target for antibody-based lung cancer diagnosis and therapy, unlocking novel avenues for early detection and treatment.

• Journal of Digestive Cancer Research
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• 제11권2호
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• pp.85-92
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• 2023

Colorectal cancer (CRC) is one of the most prevalent cancers and is the leading cause of cancer-related mortality worldwide. Based on the current screening guidelines by the American Cancer Society and Korean multi-society expert committee, CRC screening is recommended in asymptomatic adults starting at the age of 45 years. Fecal immunochemical test-based screening programs reduce the development of CRC and related mortality in the general population. However, this most popular CRC screening strategy demonstrates a crucial limitation due to modest diagnostic accuracy. Colonoscopy may be considered as an alternative primary method for CRC screening; however, its implementation can still be challenging due to concerns regarding invasiveness, low adherence, cost-effectiveness, and quality assurance. To overcome the limitations of current screening tests, innovative noninvasive tests for CRC screening have been developed with advances in molecular biology, genetics, epigenetics, and microbiomics for detecting CRC, which may enhance the approach to CRC screening and diagnosis in clinical practice in the near future. This review explores the emerging screening methods and discusses their potential for integration into current practice.

• 대한세포병리학회지
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• 제17권1호
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• pp.38-45
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• 2006

Bile duct brush cytology has been employed as a diagnostic tool for the evaluation of pancreatic and biliary tract strictures. The specificity of this method is high however, its sensitivity is quite low. A recent study employing liquid based cytology (LBC) reported results comparable to those achieved via conventional cytology. Therefore, we have attempted to prospectively evaluate the diagnostic utility of bile duct brush cytology in pancreaticobiliary diseases. A total of 46 cases with bile duct stricture were enrolled including 11 cases of benign stricture, 29 cases of bile duct carcinoma, 3 cases of gallbladder cancer, and 3 cases of pancreatic cancer. Both conventional smear and LBC using $MonoPrep2^{TM}$ system were conducted in each case. The cytological diagnosis of each case was classed into the following categories; benign, suspicious for malignancy, and malignancy. The diagnostic accuracy of both cytologic methods was investigated. LBC evidenced a high rate of material insufficiency (13/46), which was attributed to low cellularity. The kappa index of both cytological methods was 0.508. Cytological and tissue diagnoses were correlated in 25 cases conducted from biopsy or operation. The sensitivity, specificity, positive predictive value, and negative predictive value were 41.2% (7/17), 100% (8/8), 100% (7/7), and 44.4% (10/18) in conventional smear; 58.8% (10/17), 87.5% (7/8), 90.9% (10/11), and 50.0% (7/14) in LBC; and 94.1% (16/17), 87.5% (7/8), 94.1% (16/17), and 87.5% (7/8) in any one of both cytological methods, respectively. Based on these results, the sensitivity of LBC was found to be superior to that of conventional smear and we were able to obtain higher positive predictive value upto 94.1% by simultaneously conducting both cytologic methods.

• Tuberculosis and Respiratory Diseases
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• 제61권6호
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• pp.547-553
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• 2006

배 경: 액상 세포학 검사법(Liquid-based cytology)은 최근들어 각광을 받고있는 검사방법으로 여러 연구, 특히 산부인과적 영역에서 암진단에 높은 효율성을 보여주고 있다. 본 저자들은 기관지내시경상 이상병변을 보이는 환자에서 세척세포진, 솔질세포진, 액상 세포학 검사법인 Thinprep을 시행하여 진단율 및 효용성을 비교하여 보았다. 방법 및 대상: 본원에서 2005년 6월부터 9월까지 호흡기적 증상을 보여 내원한 환자 중 기관지내시경을 시행하여 병변이 발견된 30명의 환자에서 Thinprep과 기존세포검사를 시행하였다. 결 과: 이상병변을 보였던 30명의 환자 중 조직검사를 통해 암을 진단받은 환자는 24명이었으며 4명은 결핵, 각각 1명씩은 기관지확장증, 기관지-폐누공을 진단받았다. Thinprep에서 암인 24명의 환자 중 19명이, 기존세포검사에서 기관지솔질검사(brushing)을 통해 얻은 결과가 17명, 세척액(washing)은 12명에서 암세포나 비전형세포가 보였다. 두 방법 모두 암이 아닌 환자에서는 비전형세포는 보이지 않았다. 세척세포진검사는 민감도 50%, 특이도 100%, 양성예측도 100%, 음성예측도 33.3%, 솔질세포진검사는 민감도 70.8%, 특이도 100%, 양성예측도 100%, 음성예측도 46.2%, Thinprep에서는 민감도 79.2%, 특이도 100%, 양성예측도 100%, 음성예측도 54%를 보였다. 결 론: 액상 세포학 검사법인 Thinprep은 기존의 검사법보다 민감도, 음성예측도가 큰 검사로 폐암의 진단률을 높이는데 도움을 줄 수 있으며 앞으로 대단위의 연구가 필요할 것으로 생각된다.

• Journal of Trauma and Injury
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• 제18권2호
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• pp.112-118
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• 2005

Background: N-Butyl Cyanoacrylate (NBCA) is a liquid embolic material that can be useful for transcatheter arterial embolization (TAE) of acute bleeding especially in patients with coagulopathy, because it does not depend on coagulation for its therapeutic effect. The aims of this study were to evaluate the clinical efficacy and safety of TAE with NBCA in acute bleeding patients. Methods: Between August 2003 and September 2004, TAE using NBCA for acute bleeding was performed in 23 patients (16 men, 7 women; mean age, 56.5years). The causes of bleeding were gastric ulcer (n=5), postoperative bleeding (n=4), post-biopsy bleeding (n=3), postpartum bleeding (n=3), duodenal ulcer (n=2), angiodysplasia (n=2), gastric lymphoma (n=1), iatrogenic injury (n=1), CMV gastritis (n=1), stab injury of the liver (n=1). TAE was performed using 1:3 mixtures of NBCA and iodized oil. The angiographic and clinical success rate, recurrent bleeding rate, procedure-related complication and clinical outcomes were evaluated. Results: The angiographic and clinical success rate was 100% and 91.3% (21/23), respectively. There was no serious ischemic complication. Recurrent bleeding occurred in 2 patients (8.7%) and they were managed with successful second TAE (n=1) and endoscopic treatment (n=1). Nine patients (39.1%) had coagulopathy at the time of TAE and clinical success rate in this group of patients was 88.9% (8/9). Conclusions: TAE with NBCA is highly effective and safe treatment modality for acute bleeding patients, especially when the patient has a coagulopathy.

• 동의생리병리학회지
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• 제34권3호
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• pp.149-158
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• 2020

The positive margins after LEEP(loop electrosurgical excision procedure) in cervical intraepithelial neoplasia are generally considered to be a risk factor for the recurrence or persistence of CIN currently. When positive margin exists, secondary LEEP or hysterectomy is performed. The aim of this study was to observe effects of Traditional Korean Medicine treatment for patients with surgical margin positive after LEEP. It was conducted retrospective chart review for 4 patients with the surgical margin positive after LEEP, who were scheduled to have secondary LEEP 3 months later. Patients were treated with herbal medicine, pharmacopuncture and herbal liquid vaginal treatment. They were followed up by cytology, colposcopy, human papillomavirus DNA test and punch-biopsy at 1, 3 and 6 months. After 3 month of treatment, three patients did not need secondary LEEP because of normal cytology, negative HPV status and normal colposcopy, while the other patient underwent secondary LEEP because of ASCUS cytology and positive high-risk HPV. After 6 month of treatment, the other patient also had normal cytology, negative HPV status and normal colposcopy and had been in fifth week of pregnancy. This study suggest that Traditional Korean Medicine treatment may be an effective to the patients with surgical margin positive after LEEP in cervical intraepithelial neoplasia.