• Mass Spectrometry Letters
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• 제4권1호
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• pp.1-9
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• 2013

Fungal biotechnology has been well established in food and healthcare sector, and now being explored for lignocellulosic biorefinery due to their great potential to produce a wide array of extracellular enzymes for nutrient recycling. Due to global warming, environmental pollution, green house gases emission and depleting fossil fuel, fungal enzymes for lignocellulosic biomass refinery become a major focus for utilizing renewal bioresources. Proteomic technologies tender better biological understanding and exposition of cellular mechanism of cell or microbes under particular physiological condition and are very useful in characterizing fungal secretome. Hence, in addition to traditional colorimetric enzyme assay, mass-spectrometry-based quantification methods for profiling lignocellulolytic enzymes have gained increasing popularity over the past five years. Majority of these methods include two dimensional gel electrophoresis coupled to mass spectrometry, differential stable isotope labeling and label free quantitation. Therefore, in this review, we reviewed more commonly used different proteomic techniques for profiling fungal secretome with a major focus on two dimensional gel electrophoresis, liquid chromatography-based quantitative mass spectrometry for global protein identification and quantification. We also discussed weaknesses and strengths of these methodologies for comprehensive identification and quantification of extracellular proteome.

• 한국초지조사료학회지
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• 제28권2호
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• pp.129-138
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• 2008

Demand for alternatives to petroleum is increasing the production of biofuels from food crops such as corn, soybeans, sorghum and sugarcane, etc. At least for the next 5 years, ethanol demand will be increased greatly in the United States and in the world. Presently, most ethanol produced in the United States is corn (Zea mays) ethanol. As a result, especially in the Americas and Southeast Asia, agricultural land is diverted to biofuel production. Even though biofuel industry has many advantage including national security, economical, energetical and sustainable impacts, it is driving grain prices up and creating considerable concern about the potential negative impacts on a wide range of food products that depend on gain : chicken, pork, beef, and dairy products such as milk, cheese, yoghurt, cream and ice cream. Feedstock crops are crops such as switchgrass(Panicum virgatum, L.), corn stover and grasses that can be used in industrial processes such as fermentation into alcohol fuels. Feedstock is no compete with food. Furthermore it is friendly environmental bioenergy crops. In Korea, with increasing demand for fossil fuels the exploration of alternative sources of liquid fuel is inevitable. I suggest Korea need to research and to develop actively on feedstock for biofuel production through this review.

• Mycobiology
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• 제49권3호
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• pp.280-284
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• 2021

Mushroom strains of Polyporales from the genera Coriolus, Trametes, Pycnoporus, Ganoderma, and Formitella were explored in terms of mycelial growth characteristics for the application of mushroom mycelia as alternative sources of materials replacing fossil fuel-based materials. Among the 64 strains of Polyporales, G. lucidum LBS5496GL was selected as the best candidate because it showed fast mycelial growth with high mycelial strength in both the sawdust-based solid medium and the potato dextrose liquid plate medium. Some of the Polyporales in this study have shown good mycelial growth, however, they mostly formed mycelial mat of weak physical strength. The higher physical strength of mycelial mat by G. lucidum LBS5496GL was attributed to its thick hyphae with the diameter of 13 mm as revealed by scanning electron microscopic analysis whereas the hyphae of others exhibited less than 2 mm. Glycerol and skim milk supported the best mycelial growth of LBS5496GL as a carbon and a nitrogen source, respectively.

• Korean Chemical Engineering Research
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• 제50권1호
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• pp.35-40
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• 2012

본 연구에서는 화석 연료 사용시 발생되는 이산화탄소를 분리할 수 있는 신 흡수제로써 활용 가능성을 파악하기 위하여, sulfonate계 이온성 액체인 1-(2-methoxyethyl)-3-methylimidazolium methanesulfonate 흡수제에 대하여 합성하고, 성상 분석 및 이산화탄소 흡수능을 측정하였다. 1단 방법을 이용하여 저렴하게 이온성 액체를 합성하였다. 합성된 시료의 열적, 화학적 안정성을 DSC 및 TGA를 사용하여 측정하였으며, 화학적 구조는 $^1H$-NMR spectrum으로 확인하였다. 또한, 가변부피투시창(Variable-volume view cell)이 장착된 고압용 상평형 장치를 사용하여 $CO_2$ 흡수능을 평가하였다. 실험 조건은 30, 50, $70^{\circ}C$ 온도에서 수행하였고, 압력 195 bar까지 측정하였다. 실험결과 압력이 증가하거나 온도가 감소할 때 $CO_2$ 흡수량이 증가하였으며, $30^{\circ}C$, 13 bar에서 27.6 $CO_2/IL$(g/kg)의 $CO_2$ 흡수능을 보였다.

• 에너지공학
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• 제16권2호
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• pp.83-92
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• 2007

석유자원 고갈에 따른 대체에너지 개발의 필요성과 더불어 온실가스인 $CO_{2}$ 저감 등 높은 환경개선 효과로 인하여 새로운 청정연료로 바이오매스로부터 제조되는 BTL(Biomass to Liquid)-디젤에 대한 관심이 유럽을 중심으로 크게 증가하고 있다. 본 논문에서는 BTL-디젤 기술 개발 현황 및 BTL 공정의 세부 공정기술들을 조사하였다. BTL 공정은 바이오매스 전처리 및 가스화, 합성가스 정제, F-T(Fischer-Tropsch) 합성 및 upgrading 공정 등으로 나눌 수 있으며, BTL 만의 차별적 기술로 합성가스 제조를 위한 가스화 공정과 함성가스 조성 조절을 포함하는 BTL 공정에 최적화된 F-T 합성 촉매 개발이 매우 중요하다. 대표적인 BTL 기술로 독일의 Choren 사는 Carbo-V 가스화 기술을 개발하여 세계 최초로 BTL 공정 상업화를 이루었으며, 네덜란드의 ECN은 tar와 BTX를 제거하기 위한 독자적인 OLGA 기술을 개발하여 가스화 시스템과 연계한 공정을 개발하였다. 또한 미국과 일본 등 많은 나라에서 경쟁적으로 기술개발을 재촉하고 있는 상황이다. 국내의 경우 이에 대한 연구는 전무하나 국내 에너지 안보를 위한 에너지 Mix 정책과 지구온난화 등 환경문제에 대응하기 위하여 BTL 기술 개발은 매우 중요한 의미를 가질 수 있으므로 독창적 기술의 선점이 매우 중요할 것으로 판단된다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.43-43
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• 2014

White rot fungi have been useful source of enzymes for the degradation of environmental pollutants including polycyclic aromatic hydrocarbons (PAHs) and synthetic dyes. PAHs are widespread organic compounds present in fossil fuels and are routinely generated by incomplete fuel combustion. PAHs are some of the major toxic pollutants of water and soil environments. Synthetic dyes are major water-pollutants, which are toxic to organisms in water environments and interfere photosynthesis of water plants. Removal of PAHs and synthetic dyes has been of interests in the environmental science especially in the environmental microbiology. Mushrooms are fungal groups that function as primary degraders of wood polyphenolic lignin. The ligninolytic enzymes produced by mushroom, including manganese peroxidase, lignin peroxidase, and laccase, mediate the oxidative degradation of lignin. The catalytic power of these enzymes in the degradation of aromatic ring compounds has been sought for the degradation of various organic compounds. In this project, we have screened 60 wild mushroom strains for their degradation activity against two representative PAHs, naphthalene and anthracene, and five aromatic dyes, including alizarin red S, crystal violet, malachite green, methylene blue, rose bengal. The degradation of PAHs was measured by GC while the decolorization of dyes was measured by both UV spectrophotometer and HPLC. As results, 9 wild mushroom strains showed high activity in degradation of PAHs and textile dyes. We also describe the secretive enzyme activities, the transcription levels, and cloning of target genes. In conjunction with this, activities of degradative enzymes, including laccase, lignin peroxidase, and Mn peroxidase, were measured in the liquid medium in the presence of PAHs and dyes. Our results showed that the laccase activity was directed correlated with the degradation, indicating that the main enzyme acts on PAHs and dyes is the laccase. The laccase activity was further simulated by the addition of $Cu^{2+}$ ion. Detailed studies of the enzyme system should be sought for future applications.

• 한국환경보건학회지
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• 제34권1호
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• pp.42-48
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• 2008

The objectives of this study were to measure ambient TGM concentrations in Seoul Korea, to determine the temporal variation of TGM, and to analyze the relationships among TGM, meteorological data and PM2.5 measured at the same time. Ambient TGM and PM2.5 concentrations were measured at the roof of the Graduate School of Public Health building in Seoul, Korea for the period of January to October 2004. Average TGM concentration was $3.43{\pm}1.17ng/m^3$. The average TGM was at a low concentration similar to those of background sites in other countries. The temporal variations and meteorological phenomena of TGM were not statistically significant. There was a positive link between TGM and PM2.5. It didn't indicate that reduction of $Hg^{2+}$ to Hg0 had occurred in liquid water contained in smog as in a previous study, but it shows that PM2.5 and TGM could be emitted from the same sources such as power plants and combustion engines. Also, the strong correlation between TGM and $SO_2$ concentrations indicated that the source of TGM was from fossil fuel combustions including coal combustion. Specifically, $SO_2\;and\;SO_4{^2-}$ concentrations correlated to TGM concentrations could be linked to TGM emitted from local and regional sources as well.

• 한국산학기술학회논문지
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• 제19권8호
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• pp.552-561
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• 2018

화석연료의 고갈과 환경문제를 대응하기 위한 대체에너지 중 재생가능하고 탄소중립(Carbon-neutral)자원인 바이오매스 (Biomass)를 연료로 이용하는 연구가 진행되고 있다. 바이오매스를 사용하는 대부분의 에너지 생산 시스템은 열화학전환방법이 대표적이다. 이 가운데 가스화 기술을 이용해 합성가스 (syngas)를 생산해 보일러나 엔진 등에 적용하여 열과 전기를 생산한다. 하지만 합성가스 (syngas)를 생산하는 과정에서 타르 (tar)가 발생되며 낮은 온도에서 응축되기 때문에 배관 및 엔진 등에 막힘 현상을 일으켜 공정 효율을 감소시키는 문제를 야기한다. 타르를 제거하기 위해 대부분의 가스화 공정에서 물을 이용한 wet scrubber를 사용하고 있는데 효율이 낮은 문제점이 있다. 이에 본 연구에서는 물과 oily material (soybean oil, waste cooking oil, mineral oil)을 이용하여 제거효율이 높은 순으로 나타내자면 Soybean oil>Waste Cooking Oil>Mineral oil>Water 순서로 나타났고 제거효율은 각각 약 97%, 약 70%, 약 63%, 약 30%의 효율을 보여주었으며 식물성 오일 종류인 soybean oil을 사용하였을 때 타르 제거 효율이 가장 높았다.

• 한국대기환경학회지
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• 제34권5호
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• pp.651-658
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• 2018

Particulate matter (PM) emitted from fossil fuel-combustion facilities can be classified as either filterable or condensable PM. The U.S. Environmental Protection Agency (EPA) defined condensable PM as material that is in the phase of vapor at the stack temperature of the sampling location which condenses, reacts upon cooling and dilution in the ambient air to form solid or liquid in a few second after the discharge from the stack. Condensable PM passed through the filter media and it is typically ignored. But condensable PM was defined as a component of primary PM. This study investigates the change of condensable PM according to the variation in the sulfur dioxide of combustion gas. Domestic oil boilers were used as the source of emission ($SO_2$) and the level of $SO_2$ concentration (0, 50, 80, and 120 ppm) was adjusted by diluting general light oil and marine gas oil (MGO) that contains sulfur less than 0.5%. Condensable PM was measured as 2.72, 6.10, 8.38, and $13.34mg/m^3$ when $SO_2$ concentration in combustion gas were 0, 50, 80, and 120 ppm respectively. The condensable PM tended to increase as the concentration of $SO_2$ increased. Some of the gaseous air pollutants emitted from the stack should be considered precursors of condensable PM. The gas phase pollutants which converted into condensable PM should reduced for condensable PM control.

• 한국수소및신에너지학회논문집
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• 제26권2호
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• pp.120-126
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• 2015

Recently, the ever-increasing use of fossil fuels for rapid industrial development and population significantly caused an environment pollution and global warming such as climate change. So research and development of sustainable and eco-friendly energy have been performed. Especially the interest in nuclear fusion fuel was significantly increased from the developed countries. The system of fusion energy production was tritium separation, storage and delivery, and purification. Republic of Korea is in charge of Storage and Delivery System (SDS) in the International Thermonuclear Experimental Reactor (ITER). Welding part of the SDS bottles for storing the tritium is known to be susceptible to hydrogen embrittlement. In this study, conducted a study for the relaxation of the stability and hydrogen embrittlement of the weld area. The hydrogen heat treatment was processed through the Pressure-Composition-Temperature (PCT) device according to the time variation. Also mechanical properties such as impact test and hardness test according to using the alkaline cleaning liquid for hydrogen embrittlement relief and the fracture was observed by scanning electron microscopy (SEM) after the mechanical properties evaluation.