• 한국작물학회지
• /
• 제42권1호
• /
• pp.1-6
• /
• 1997

근래에 육성된 한국산 녹두품종에 대하여 녹두 용도별 가공 적성분류 기준이 될 수 있는 주요 일반성분 함량과 지방산 조성을 조사하여 품종 육성의 기초 자료로 제공하고자 수행하였던 바 그 결과를 요약하면 다음과 같다. 1. 평균 조단백질, 조지방 및 조회분 함량은 각각 24.3, 3.6％였는데 수분함량은 약 10％ 정도였다. 2. 조단백질과 조탄수화물간, 종자수분과 조지방함량간, 종실중과 조지방함량간 등에서는 각각 고도의 부의 유의조관을 보였다. 3. 지방산조성 중 불포화지방산이 60∼67％ 함유되었으며 종류는 oleic acid, linoleic acid, linolenic acid, arachidonic acid 등 4종이었고, 포화지방산이 33∼40％정도 함유되었는데 종류는 stearic acid, palmitic acid, behenic acid, lignoceric acid 등 4종이었다. 4. 지방산 조성 중 linoleic acid가 33.46％로 가장 많았으며, 다음이 linoenic acid와 palmitic acid로 각각 21.87, 21.72％였고, 다음이 stearic acid, oleic acid, arachidonic acid가 각각 5.98, 5.87, 4.87％ 였으며 behenic acid와 lignoceric acid는 극히 미량 존재하였다. 5. Palmitic acid와 linoleic acid, oleic acid와 lignoceric, behenic acid간에는 고도의 정상관관계를, palmitic acid와 arachidonic, lignoceric acid간, oleic acid와 linolenic acid, arachidonic acid와 lignoceric acid간에는 고도의 부의 상관관계를 보였다. 6. 종실중과 oleic, arachidonic, behenic acid간에 고도의 정상관관계를 보였다.

• 한국식품영양과학회지
• /
• 제26권6호
• /
• pp.1033-1038
• /
• 1997

Antioxidant effect of several antioxidative components on the high purity $\alpha$-linolenic acid(HALA; ALA, 78.1%) ethyl ester concentrated from perilla oil were investigated by measuring weight-gains and peroxide value(POV) during storage at 5$0^{\circ}C$, 23$^{\circ}C$ and 4$^{\circ}C$. Amounts of antioxidant components were 0.2g/kg HALA ethyl ester for sesamin, sesangolin and butylated hydroxytolune(BHT), and 0.1g/kg for sesamol and 100g/kg for ether extracts from perilla seed. The oxidative stability of HALA ethyl ester was particularly increased by adding sesamol, ether extracts and BHT, but sesamin and sesangolin scarcely showed an antioxidant effect. POV on the HALA ethyl ester added sesamol and ether extract was less than 15.0meq/kg by 9 weeks of storage at 23$^{\circ}C$. However, in the case of low temperature storage at 4$^{\circ}C$, all the samples estimated showed less than 7.0meq/kg in POV by 5 months. Consequently, sesamol and ether extracts were recognized as available antioxidant components on the HALA ethyl ester from perilla oil.

• 한국식품과학회지
• /
• 제17권5호
• /
• pp.371-376
• /
• 1985

내한유채를 $25^{\circ}C$에서 60시간동안 발아시켜 건물량, 뿌리신장도, 아미노산 및 지방산조성의 변화를 조사한 결과는 다음과 같다. 유채의 건물량은 발아30시간에서 비교적 급격한 감소를 나타냈고 그 이후는 완만한 감소를 나타냈으며, 우리는 15시간까지는 관찰되지 않았으나 발아30시간이후는 일정한 속도의 신장도를 나타낸다. 유채는 발아함에 따라 수분, 조회분은 큰 영향을 받지 않았으나 조지방 조단백질은 감소하였으며 조섬유는 증가하였다. 유채의 아미노산은 Glu, Lys, Asp가 많고 lie, Met가 적었으며 제 1제한 아미노산은 Ile 이었다. 유채의 아미노산은 발아함에 따라 Lys, His, Arg, Asp, Thr, Ser, Ala, Val, lie, Leu는 증가하였지만 Clu, Met Tyr, Phe은 감소하여 발아함에 따라 Met이 제 1제한아미노산이 되었다. 지방산은 oleic acid (49.3%). linoleic acid(22.0%), eicosenoic acid (10.5%), Palmitic acid(5.9%), linolenic acid(5.7%), stearic acid(3.2%), erucic acid(2.8%)가 검출되었는데. 발아에 따라 oleic acid는 감소하였으며 Palmitic acid, linolenic acid 발아중기까지 증가하다가 그 이후는 감소하였고 linolenic acid eicosenoic acid 및 erucic acid는 발아중기까지는 감소하다가 그 이후는 증가하였다.

• 한국식품영양과학회지
• /
• 제19권6호
• /
• pp.636-640
• /
• 1990

For a studyon the production of ${\gamma}$-linolenic acid(GLA) by fungi 3 fungal strains were isolated from soil. Their cell growth lipid content and fatty acid composition were compared in shake flask culture. Among these fungi the fungus designated as FA-007 has high lipid content(21.1%) and GLA content(15.6% of total fatty acids) The fungal strain FA-007 was tentatively identified as Mucor sp. on the basis of morphological characteristics, Fungal oil produced by this fungus was composed of 75.2% neutral lipid 5.3% glycolipid and 19.5% phospholipid. Although the GLA content in phospholipid was higher than it in neutral lipid the GLA content in neurtal lipid was high as 15.5%.

• Asian-Australasian Journal of Animal Sciences
• /
• 제14권8호
• /
• pp.1110-1117
• /
• 2001

Responses of the rumen fungus, Neocallimastix frontalis RE1, to long chain fatty acid (LCFA) were evaluated by measuring gas production, filter paper (FP) cellulose digestion and polysaccharidase enzyme activities. LCFA (stearic acid, $C_{18:0}$; oleic acid, $C_{18:1}$; linoleic acid, $C_{18:2}$ and linolenic acid, $C_{18:3}$) were emulsitied by ultrasonication under anaerobic condition, and added to the medium. When N frontalis RE1 was grown in culture with stearic, oleic and linoleic acid, the cumulative gas production, gas pool size, FP cellulose digestion and enzymes activities significantly (p<0.05) increased at some incubation times(especially, exponential phases of fungal growth, 48~120 h of incubation) relative to that for control cultures. However, the addition of linolenic acid strongly inhibited all of the investigated parameters up to 120 h incubation, but not after 168 and 216 h of incubation. These results indicated that stearic, oleic and linoleic acids tended to have great stimulatory effects on fungal cellulolysis, whereas linolenic acid caused a significant (p<0.05) inhibitory effects on the cellulolysis by the rumen fungus. These results are the first report of the effect of LCFAs on the ruminal fungi. Further research is needed to identify the mode of action of LCFAs on fungal strains and to verify whether or not ruminal fungi have ability to hydrate unsaturated LCFAs to saturated FAs. There was high correlation between cumulative in vitro gas production and fungal growth (94.78%), FP cellulose degradation (96.34%), CMCase activity(90.86%) or xylanase activity (87.67%). Thus measuring of cumulative gas production could be a useful tool for evaluating fungal growth and/or enzyme production by ruminal fungi.

• KSBB Journal
• /
• 제26권6호
• /
• pp.557-560
• /
• 2011

It has been known that Δ-desaturase (TAD5) in the biosynthetic pathway of long chain polyunsaturated fatty acids of Thraustochytrium aureumis responsible for the conversion of di-homo-${\gamma}$-linolenic acid (C20：4) into arachidonic acid (C20：4). The genetic sequence analysis on TAD5 of Thraustochytrium aureum ATCC34304 used in this study showed that it has two amino acid changes when compared to that of Thraustochytrium aureum TAD5 first reported in 2003. Accordingly, Thraustochytrium aureum ATCC34304 TAD5 was named TAD5_1. TAD5_1-inserted methylotropic Pichia pastoris was prepared and then cultured with a precursor fatty acid, di-homo-${\gamma}$-linolenic acid. GC analysis confirmed that a certain amount of the precursor fatty acid was converted into arachidonic acid. In this study, not only a recombinant Pichia pastoris with the typical activity of ${\Delta}5$-desaturase which plays an essential role in the biosynthesis of LCPUFAs was successfully made but also the preparationpotential of a recombinant Pichia pastoris strain which may synthesize eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) that are important in maintaining and improving human's brain function was proposed.

• 대한화학회지
• /
• 제30권4호
• /
• pp.403-405
• /
• 1986

• 한국식품영양학회지
• /
• 제23권4호
• /
• pp.516-525
• /
• 2010

아마인의 유용성분에 대해서 알아보고 가열과정을 통한 성분변화 및 다른 종유작물과 유지의 물리화학적 성분과 비교, 평가하였다. 조지방은 37~43%, 탄수화물은 30~35%, 조단백질은 18~23%, 조회분은 3~4% 그리고 수분 함량은 0.2~6.8%의 함량을 나타내었다. 식이섬유 함량은 생아마인보다 가열 처리한 볶음 아마인에서 SDF의 함량이 증가한 것을 알 수 있었다. 하지만 상대적으로 IDF의 함량도 감소하기 때문에 TDF의 함량은 큰 차이를 보이지 않았다. 그리고 총 식이섬유 함량은 28~31%로 높은 함량을 보였다. 또한 아마인의 주요 유용성분을 살펴본 결과, 비타민은 ${\gamma}$-tocopherol(E), 아미노산은 agrginine, aspartic acid, glutamic acid가 높은 함량을 보였다. 이들은 생아마인보다 볶음 아마인에서 낮은 함량을 나타내었다. 아마인의 무기물 함량을 분석한 결과, 칼슘, 칼륨, 마그네슘, 인이 주요 구성성분이었다. 물리화학적인 특징으로 요오드가, 비누화가, 산가를 분석하였으며, 브라운 아마인유는 각각 204.1 g/100 g, 193.6 mg/g, 1.59 mg/g을 나타내었으며, 골드 아마인유는 각각 203.0 g/100 g, 189.9 mg/g, 2.35 mg/g으로 조사되었다. 아마인유의 지방산 함량에서는 전체적인 구성 비율을 볼 때 ${\alpha}$-linolenic acid(ALA, C18:3n-3)가 약 55.5~56.1% 로 가장 높은 함량을 차지하였다. 그리고 브라운보다는 골드 아마인유의 ${\alpha}$-linolenic acid의 함량이 약간 높았다. 높은 ${\alpha}$-linolenic acid 함량은 아마인유가 오메가-3 지방산이 강화된 식품 개발에 좋은 자원이 될 것으로 사료된다. 또한 다가 불포화지방산 함량이 약 71%로 함유되어 있으며, ${\alpha}$-linolenic acid 등 필수지방산의 함량이 높아 식품의 영양학적인 의의가 크며, 양질의 식용유로 사용 가능할 것이다.

• 한국토양비료학회지
• /
• 제15권4호
• /
• pp.251-257
• /
• 1982

광조사시간(光照射時間)에 따른 대두유식물(大豆幼植物)의 지방산(脂防酸)과 sterol 조성변화를 경시적으로 분석(分析)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 발아생육기간(發芽生育期間)동안 광조사시간(光照射時間)이 길수록 배축부(胚軸部)의 linoleic acid와 linolenic acid조성(組成)은 증가하였고 palmitic과 stearic acid는 감소(減少)하였다. 2. 발아후(發芽后) 2 일(日)째 근부(根部)의 palmitic과 linolenic acid는 광조사시간(光照射時間)이 길수록 감소(減少)하였고, stearic과 oleic acid는 증가하였다. 3. 발아생육기간(發芽生育期間)동안 자엽부(子葉部)에서 stigmasterol이 주(主)sterol이었고 배축부(胚軸部)와 근부(根部)에서는 sitosterol이 주(主)sterol이었다. 4. 발아후(發芽后) 6 일(日)째의 광조사(光照射) 24시간(時間)에서, 배축부(胚軸部)의 sitosterol이 가장 높았다. 5. 암상태(暗狀態)와 광조사(光照射) 24시간(時間)에서 근부(根部)의 sitosterol조성(組成)은 생육기간(生育期間)이 경과할수록 감소(減少)하였고, 광조사(光照射) 16시간(時間)에서는 sitosterol조성(組成)이 증가하였다.

• Journal of Nutrition and Health
• /
• 제27권10호
• /
• pp.967-978
• /
• 1994

This study was done to investigate the effect of age and dietary linolenic acid content and the linolenic acid/linoleic acid(LNA/LA) ratio on the lipid metabolism and formation of PGI2 and TXA2. The male Sprague-Dawley rats were fed 6 different with 0.2, 0.4, 0.6 of LNA/LA ratio within either 8% LNA(high LNA) or 4% LNA(low LNA) of fatty acid content for different feeding period(1, 4, 12 month). The dietary fat used were sesame oil, perilla oil, soybean oil and beef tallow. The concentration of serum total lipid, total cholesterol and HDL-C were increased with aging. Triglyceride concentration was decreased in 0.2 ratio of LNA/LA. The lipid content of liver showed similar tendency to that of serum. The ratio of PGI2/TXA2 was increased in 1 month rats and antithrombotic effect was reduced significantly with increasing age. The PGI2/TXA2 ratio was tended to be higher in diet of 0.2 and 0.4 LNA/LA ratio at high LNA level and in diet of 0.6 LNA/LA ratio at low LNA level. Especially PGI2/TXA2 ratio was increased linearly with rising LNA/LA ratio at low LNA level. It seemed that the LNA content and LNA/LA ratio had interaction to increase the antithrombotic effect bychanging TXA2 synthesis. And the dietary fatty acid related effect lowering the serum and liver lipid content, excepting triglyceride, was increased when dietary n3/n6 ratio was high(0.6) at both high and low n3 level. Therefore, it could not be recommended to consume large amount of n3 fatty acid or high ratio of n3/n6 to prevent cardiovascular diseases. These results suggested that the dietary fatty acid ratio of n3/n6 could be determined based on the n-3 content of dietary fat to reduce the risk of cardiovascular disease.