• 한국식품위생안전성학회지
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• 제16권1호
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• pp.41-47
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• 2001

녹차카테킨(GTC)의 항산화 작용을 알아보고자 in vitro와 in vivo에서 지질과산화와 superoxide dismutase(SOD), catalase 활성에 미치는 영향에 대한 실험을 행하였다. In vitro 시험계에서의 항산화활성 실험결과, GTC는 peroxide value와 과산화지질 생성을 유의성 있게 억제시켰고, SOD 활성이 매우 높았다. 또한 GTC를 rat에 경구투여 한 후 항산화활성실험 결과, GTC는 $CCl_4$로 유도된 rat의 간 microsome의 지질과산화를 유의성 있게 억제시켰으며, SOD와 catalase 활성을 유의성 있게 증가시켰다. 따라서 GTC는 암과 노화의 예방과 관련이 되는 항산화 활성이 있는 것을 알 수 있었다.

• Preventive Nutrition and Food Science
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• 제19권1호
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• pp.27-33
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• 2014

Hydrolysates of Trianthema portulacastrum in acidified methanol were evaluated for their total phenolic (TP) constituents and respective antioxidant activities using in vitro assays (i.e., 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, percent inhibition of linoleic acid peroxidation, and ferric reducing power). The observed results indicate that root, shoot, and leaf fractions of T. portulacastrum contain 50.75~98.09 mg gallic acid equivalents/g dry weight of TP. In addition, these fractions have substantial reducing potentials (0.10~0.59), abilities to inhibit peroxidation (43.26~89.98%), and DPPH radical scavenging capabilities ($6.98{\sim}311.61{\mu}g/mL$ $IC_{50}$). The experimental data not only reveal T. portulacastrum as potential source of valuable antioxidants, but also indicate that acidified methanol may be an ideal choice for the enhanced recovery of phenolic compounds with retained biological potential for the food and pharmaceutical industry.

• Preventive Nutrition and Food Science
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• 제9권2호
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• pp.167-173
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• 2004

The aim of this study was to investigate the antioxidant effect of medicinal plants used by diabetic patients. Fourteen medicinal plants were selected and antioxidant activity such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, inhibition effect of linoleic acid autoxidation and low density lipoprotein (LDL) oxidation, thiobarbituric acid (TBA) value were measured. The Cornus officinalis had DPPH radical scavenging activity of 84.79%, which was higher than the 78.79% for $\alpha$-tocopherol. Rosa rugosa Thunberg, Pueraria thundergiana Bentham, Artemisia princeps var. orientalis and Sasamorpha purpurascens Nakai also had high values. Extracts with higher DPPH radical scavenging activities had higher total phenol concentrations, and positive correlations between these parameters were found. Inhibitory activities of linoleic acid autoxidation, LDL oxidation and TBA value, used as indices of oxidative stress, were observed in most of the selected medicinal plants. The highest inhibitory activity for TBA value was observed in the extract of Pueraria thundergiana Bentham. Rosa rugosa Thunberg (75.50 %), Sasamorpha purpurascens Nakai (74.00 %), and Cornus officinalis (73.00 %) all had high antioxidant activity against linoleic acid autoxidation, similar to that of $\alpha$-tocopherol. The same 3 plants also had high in inhibitory activity against LDL oxidation. Therefore, we demonstrated that medicinal plants used as folk remedy by diabetic patients had antioxidant activity. Especially, Rosa rugosa Thunberg, Cornus officinalis, Pueraria thundergiana Bentham, Arlemisia princeps var. orientalis, and Sasamorpha purpurascens Nakai had high phenol concentrations which resulted in high values for DPPH radical scavenging. These same plants exhibited high values for inhibitory activities for TBA value, linoleic acid autoxidation and LDL oxidation, indices associated with lipid peroxidation.

• 한국응용과학기술학회지
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• 제32권3호
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• pp.363-371
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• 2015

n-Butanol and ethyl acetate extracts of thermal treatment yam (Dioscorea batatas $D_{ECNE.}$) belonging to the family Dioscoreaceae were measured for their radical scavenging activity and lipid peroxidation inhibition ability. In this study, ethyl acetate extract showed the most potent antioxidant activity evaluated by ferrous ion chelating activity and NO radical scavenging activity. Nevertheless, n-butanol extract was more effective in inhibiting linoleic acid peroxidation. A significant difference between n-butanol extract and ethyl acetate extract in nitrite scavenging activity ${\beta}$-carotene bleaching assays could not be found. Also, the results of this study showed that thermal treatment yam could be used as easily accessible source of natural antioxidants and as a possible food supplement.

• 대한한방내과학회지
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• 제23권1호
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• pp.57-64
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• 2002

Objective : This study was designed to investigate the anti-oxidative effects of Oldenlandiae Diffusae Herba Water extract (ODHW) on lipid peroxidation by free radicals oxidative hepatic injury. Methods : In order to evaluate anti-oxidative activities of ODHW in the liver cell, cultured normal rat liver cells(Ac2F) were incubated with or without ODHW. After 16 hours to 18 hours of experiment, cells were placed in DMEM medium without serum, and then incubated with 1mM tert-butyl hydro-peroxide(t-BHP) for two hours. Viable cells were detected by MTT assay. The levels of LPO induced by hydroxyl radical derived from H2O2-Fe2+ system in rat liver homogenate were determined by means of TBA. Inhibitory effect of ODHW on superoxide generation was measured by xanthine-xanthine oxidase system. Results : In the linoleic acid autoxidation system, ODHW exhibited antioxidant activity, which inhibited 85% of linoleic acid peroxidation. These effects were similar to those of dl-a-tocopherol. ODHW showed scavenging effects on DPPH radical, inhibited superoxide generation in xanthine-xanthine oxidase system, and also inhibited lipid peroxidation of rat liver tissue with hydroxyl radical derived from $H_2O_2-Fe^{2+}$ system. In addition, ODHW protected the cell death induced by t-BHP and it significantly increased cell viability in a normal rat liver cell(Ac2F)

• 생명과학회지
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• 제28권5호
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• pp.540-546
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• 2018

그라비올라(Annona muricata) 잎으로부터 정제수, 에탄올, 메탄올로 추출하여 수율, 총 페놀성 화합물과 이에 속하는 flavonoid 함량, 미네랄 함량을 측정하고 DPPH free radical scavenging 활성, Cu/Fe 환원력, 간 조직의 microsome 생체막의 지질과산화 억제능 및 linoleic acid를 이용한 지질과산화, ${\beta}$-Carotene 탈색화 정도를 비교검토하였다. 그라비올라 잎의 수율은 수용성 추출물이 가장 높았으며(1.76%), 총 페놀성 화합물 함량은 그라비올라의 메탄올 추출물에서 가장높았으나 페놀성 화합물에 속하는 폴리페놀 함량은 에탄올 추출물에서 가장 높은 결과를 나타내었고, 미네랄 함량은 Ca, K, Mg, Na, Mn의 순으로 높게 측정되었다. DPPH를 이용한 radical 소거능을 측정한 결과, 그라비올라 잎의 에탄올과 메탄올 추출물에서 대조구인 BHT 0.05%와 가장 근접한 활성을 나타내었다. Fe와 Cu의 환원력은 모든 군에서 농도 의존적으로 증가하였고, 그 중 에탄올과 메탄올 추출물에서 Cu/Fe 환원력 활성이 매우 강하였다. 그러나 간 조직을 이용하여 측정하는 지질과산화 억제활성과 TBA법으로 지질과산화를 측정한 결과, 그라비올라 잎의 에탄올 추출물에서 지질과산화 억제능을 보였다. ${\beta}$-Carotene bleaching에서 높은 lipid peroxyl 라디칼 소거 활성이 있음을 확인할 수 있었다. 따라서, 그라비올라 잎은 기능성 식품의 개발을 위한 천연 항산화제 소재로서 활용 될 수 있을 것으로 판단되어진다.

• 생명과학회지
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• 제19권11호
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• pp.1553-1561
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• 2009

홍국발효홍삼의 알코올성 간 질환 개선용 건강기능식품 개발을 위한 기초 자료를 얻기 위하여 일반 이화학적 특성과 항산화 활성 정도를 측정 하였다. 홍삼 및 홍국발효홍삼의 추출물 수율은 각각 수용성 5.83% 및 6.58%, 70% 에탄올 0.98% 및 0.62%, 70% 메탄올 3.04% 및 1.27%로 수용성 추출에서 가장 높았다. 홍삼 및 홍국발효홍삼 1% 추출물 중의 총 당도는 각각 0.23% 및 0.17%로 홍삼에서 약간 높았으며, 가용성 고형분도 각각 0.6 및 0.5 Brix로 홍삼에서 약간 높았다. 총 폴리페놀 화합물 함량은 홍삼보다 홍국발효홍삼 추출물에서 높았으며, 70% 에탄올 추출물에서 가장 높았다. 미네랄 함량은 K가 22,386 및 16,936 ppm으로 가장 많이 함유되어 있었고, 다음으로 Ca이 각각 3,693 및 2,310 ppm으로 함유되어 있었고, Mg는 각각 2,647 및 2,703 ppm, Na는 각각 103 및 109 ppm, Fe는 각각 115 및 42 ppm, Zn은 각각 57 및 62.3 ppm이 함유되어 있었다. 항산화 실험에서 총 폴리페놀 함량, DPPH radical 소거능 및 불포화 지방산 linoleic acid를 이용한 TBA법에 의한 항산화 활성은 홍삼 추출물 보다는 홍국발효홍삼에서 높은 것으로 나타났다.

• 사상체질의학회지
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• 제13권1호
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• pp.51-60
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• 2001

Effects of Taeumjowetang on Lipid Peroxidation by Free Radicals and Oxidative Damage of Hepatocytes by tert-Butyl Hydroperoxide. 1. Purpose The present study was carried out to evaluate the antioxidant effects of Taeumjowetang in vitro. 2. Methods In this study, antioxidant effects of TJT on lipid peroxidation were determined according to the method of TBA. (Abbreviation) TJT : Taeumjowetang, TBA : 2-thiobarbituric acid. 3. Results : 1) TJT inhibited markedly peroxidation of linoleic acid during the autoxidation. 2) TJT inhibited lipid peroxidation induced by hydroxyl radical derived from H2O2-Fe2+ in rat liver homogenate. 3) TJT showed 66% scavenging effect on DPPH radical. 4) TJT exhibited a 25% inhibitory effect on superoxide generation from xanthine-xan thine oxidase system. 5) To investigate the antioxidative effects of TJT on the hepatocytes, cultured normal rat liver cells(Ac2F) were prepared and incubated with or without TJT. After 16~18hr, cells placed in DMEM medium without serum, and then incubated with 1mM t-BHP for 2hr. Viable cells were detected by MTT assay. In this test, TJT protected the cell death induced by t-BHP and significantly increased cell viability in the normal rat liver cell. (Abbreviation) DPPH : ${\alpha},{\alpha}$-diphenyl-${\beta}$-picryl hydrazyl, DMEM : Dulbecco's Modified Eagle Medium, t-BHP : terr-butyl hydroperoxide, 4. Conclusion These results suggested that TJT might play a protective role in lipid peroxidation by free radicals.

• 생명과학회지
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• 제21권7호
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• pp.1052-1059
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• 2011

가시오가피 잎, 뿌리, 줄기 및 열매의 수용성, 에탄올 및 메탄올 추출물의 생리활성 물질 분석과 항산화 활성(DPPH free radical scavenging 활성, Cu/Fe-환원력, 간 조직 microsome 생체막 및 linoleic acid 과산화지질, Tyrosinase 저해활성)을 측정 하였다. 가시오가피 잎의 열수 추출물에서 추출 수율 16.04%, 폴리페놀 화합물 함량2.67% 및 플라보노이드 함량 1.43%로 가장 높았다. 가시오가피 잎, 뿌리, 줄기 및 열매의 주요 미네랄은 K, Ca 및 Mg 이었다. DPPH free radical scavenging 활성, Cu/Fe-환원력, 간 조직 microsome 생체막 및 linoleic acid의 과산화 지질 측정에 의한 항산화 활성은 가시오가피 잎, 뿌리, 줄기 및 열매 모두에서 나타났으며, 시료 처리 농도 의존적으로 활성이 증가되는 것으로 나타났다. 이상의 실험 결과에서 가시오가피 잎, 뿌리, 줄기 및 열매 모두에서 높은 항산화 활성이 있었으며, 이는 폴리페놀 화합물과 플라보노이드와 같은 생리활성 성분을 많이 함유하고 있는 것과 깊은 관련성이 있는 것으로 향후 건강기능식품이나 기능성화장품의 천연 항산화제 소재 개발 관련 연구의 기초자료로 활용될 수 있을 것으로 사료되어 진다.

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1524-1528
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• 2010

The antioxidant properties of 46 lichen species, collected from the highly UV-exposed alpine areas of southwestern China, were evaluated for their potential therapeutic utilization. The anti-linoleic acid peroxidation activity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity, reducing power, and total phenolic contents were all assessed in vitro in the methanol extract of the lichens. A potent reducing power was detected in a number of the lichen extracts, when compared with butylated hydroxyanisole (BHA). In general, it was found that many of the lichens, with antioxidant properties, contained large quantities of phenolic content. Extracts of Peltigera praetextata and Sticta nylanderiana were found to exhibit the most potent activity in all of the antioxidant tests. In particular, extracts of S. nylanderiana displayed a 1.37 times greater anti-linoleic acid peroxidation activity, when compared with the ascorbic acid used as the positive control. S. nylanderiana also possessed the strongest free radical scavenging activity amongst all the tested species, with an inhibition rate of 90.4% at concentration of $330{\mu}g/ml$. Activity-guided bioautographic TLC and HPLC analyses were used to establish which compounds were responsible for the potent antioxidant activities of the S. nylanderiana extract. These analyses revealed lecanoric acid to be primarily responsible for the effective antioxidant properties of S. nylanderiana. Overall, these results have indicated that several highland lichens have the potential of being utilized as novel bioresources for naturally occurring antioxidant therapies.