• 한국식품과학회지
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• 제29권5호
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• pp.925-931
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• 1997

전갱이의 조미 반건품을 대량으로 생산하기 위한 기초자료를 얻기 위해, 전갱이를 조미 반건조한 후 나일론+폴리에틸렌+선형 저밀도폴리에틸렌$(0.015/0.045/0.04\;{\mu}m)$의 적층필름으로 진공포장하여 빙결점 동결$(-3{\pm}0.5^{\circ}C)$ 저장하면서 저장 중의 품질변화를 비교 검토하였다. 전갱이 조미 반건품을 제조하기 위한 가장 적합한 건조온도 및 시간은 상대습도 $50{\pm}5%$, 풍속 2m/sec 조건에서 각각 $20^{\circ}C$ 및 4시간이었으며 진공포장제품의 저장기한은 42일이었고 비포장제품의 경우는 28일이었다.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.453-464
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• 2014

The current research was focused on the extracellular biosynthesis of bactericidal silver nanoparticles (AgNPs) using cell-free supernatant of a local isolate previously identified as a novel Streptomyces aegyptia NEAE 102. The biosynthesis of silver nanoparticles by Streptomyces aegyptia NEAE 102 was quite fast and required far less time than previously published strains. The produced particles showed a single surface plasmon resonance peak at 400 nm by UV-Vis spectroscopy, which confirmed the presence of AgNPs. Response surface methodology was chosen to evaluate the effects of four process variables ($AgNO_3$ concentration, incubation period, pH levels, and inoculum size) on the biosynthesis of silver nanoparticles by Streptomyces aegyptia NEAE 102. Statistical analysis of the results showed that the linear and quadratic effects of incubation period, initial pH, and inoculum size had a significant effect (p < 0.05) on the biosynthesis of silver nanoparticles by Streptomyces aegyptia NEAE 102. The maximum silver nanoparticles biosynthesis (2.5 OD, at 400 nm ) was achieved in runs number 5 and 14 under the conditions of 1 mM $AgNO_3$ (1-1.5% (v/v)), incubation period (72-96 h), initial pH (9-10), and inoculum size (2-4% (v/v)). An overall 4-fold increase in AgNPs biosynthesis was obtained as compared with that of unoptimized conditions. The biosynthesized silver nanoparticles were characterized using UV-VIS spectrophotometer and Fourier transform infrared spectroscopy analysis, in addition to antimicrobial properties. The biosynthesized AgNPs significantly inhibited the growth of medically important pathogenic gram-positive (Staphylococcus aureus) and gram-negative bacteria (Pseudomonas aeruginosa) and yeast (Candida albicans).

• 한국수산과학회지
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• 제36권6호
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• pp.716-722
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• 2003

This study determined the optimum dosage for coagulation reactions of red tide organisms (RTO) using a combination of ignited oyster shell powder (10sp) and loess and examined the electrokinetic and rheological characteristics of their flocs. Two kinds of RTO, Cylindrotheca closterium and Skeletonema costatum, were sampled in Masan Bay and cultured in the laboratory. Coagulation experiments were conducted using various concentrations of IOSP, loess, IOSP+1oess, RTO, and a jar tester RTO cell numbers were counted for both the supernatant and RTO culture solution. The removal rates increased rapidly with increasing IOSP concentrations up to 50 mg/L and loess concentrations up to 800 mg/L. A removal rate of $100\%$ was reached at 400 mg/L of IOSP and 6,400 mg/L of loess. The highest increment $(16.7\%)$ of the rates of coagulation reaction occurred using both IOSP and loess (50+200 mg/L) in comparison with IOSP alone. The rate of coagulation reaction using both IOSP and loess (50+200 mg/L), $90.6\%,$ was similar to employing either IOSP of 150 mg/L or loess of 3,200 mg/L. All of the coagulation liquids for RTO, IOSP (200 mg/L), loess (200 ma/L), and IOSP+1oess (200+200 mg/L) revealed non-Newtonian fluid properties and therefore their shear rate vs. shear stress curves were non-linear. The coagulation liquids revealed elastic body properties at a lower shear rate increasing in the following order: RTO, IOSP (200 mg/L), loess (200 mg/L), and IOSP+1oess (200+200 mg/L. IOSP+1oess (200+200 mg/L) especially demonstrated plastic flow properties at a lower shear rate.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2013년도 추계학술대회
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• pp.346-349
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• 2013

본 논문에서는 MPPT(Maximum Power Point Tracking) 제어기능을 갖는 빛, 진동 그리고 열 에너지를 이용한 삼중입력 에너지 하베스팅 회로를 설계하였다. 설계된 회로는 MPPT 제어를 통해 솔라셀과 압전소자 그리고 열전소자로부터 최대 가용 전력을 수확하고, 수확된 에너지를 저장 커패시터에 병합하여 저장한다. 병합된 에너지는 PMU(Power Management Unit)를 통해 센서노드로 공급된다. MPPT 제어는 변환소자의 개방회로전압과 MPP(Maximum Power Point) 전압간의 비례관계를 이용하여 구현하였다. 제안된 회로는 $0.18{\mu}m$ CMOS 공정으로 설계하였으며, 모의실험을 통해 동작을 검증하였다. 설계된 에너지 하베스팅 회로의 칩 면적은 $945{\mu}m{\times}995{\mu}m$이다.

• 한국통신학회논문지
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• 제30권12C호
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• pp.1193-1200
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• 2005

본 논문에서는 JPEG2000 표준에서 주파수 변환기법으로 채택된 이산 웨이블릿 변환과 선형양자화 방법을 사용하여 영상 전체가 아닌 영상의 부분 데이터만을 암호화하여 계산양을 줄이는 부분 암호화 방법을 제안하고 하드웨어로 구현하였다. 또한 계산양이 많은 암호화 알고리즘 대신 비교적 계산양이 적은 카오스 시스템을 이용함으로써 계산양을 더욱 감소시킨다. 영상 데이터의 변환 방법은 암호화할 부대역을 선택하여 영상데이터를 일정한 블록으로 만든 후 무작위로 좌/우 쉬프트 하는 방법과 두 가지 양자화 할당 방식(하향식-코드 할당방식/반향-코드 할당방식)에 따라 데이터를 교환하는 방식을 사용한다. 제안한 암호화 방법을 소프트웨어로 구현하여 약 500개의 영상을 대상으로 실험한 결과 원 영상 데이터를 부분적으로 암호화함으로써 원 영상을 인식할 수 없을 정도의 암호화효과를 얻을 수 있음을 알 수 있었다. 구현한 하드웨어 암호화 시스템은 삼성 $0.35{\mu}m$ 팬텀-셀 라이브러리를 사용하여 합성함으로써 게이트 수준 회로를 구성하였고 타이밍 시뮬레이션을 수행한 결과 100MHz 이상의 동작 주파수에서 안정적으로 동작함을 확인하였다.

• Corrosion Science and Technology
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• 제7권6호
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• pp.319-323
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• 2008

The polarization resistance of mild steel in 0.5M hydrochloric acid has been evaluated by using impedance (Z) and linear polarization (LPR) techniques and compared to the noise resistance obtained from electrochemical noise data. The degree of localization of this general corrosion has also been discussed by evaluating localization index and power spectral density. Polarization resistance obtained by LPR technique ($28\Omega$) was higher than that obtained by impedance technique ($15\Omega$). Noise resistance ($11\Omega$) was much lower than polarization resistance measured by both of above techniques. Higher polarization resistance obtained by LPR technique is generally caused by passivation effect in the presence of scales or deposits which can introduce an increased resistance as can low conductivity electrolytes. The reason why noise resistance is lower than polarization resistance is the effect of background noise detected by using three platinum electrodes cell in 0.5M hydrochloric acid. Slope($-\beta$) of power spectral density (PSD) obtained from analysis of noise data ($-\beta$ = 3.3) was much higher than 2 which indicates mild steel corroded uniformly. Localization index (LI) calculated from statistical analysis (LI=0.08) is much lower than 1 which indicates that mild steel did not corroded locally. However, LI value is still higher than $1x10^{-3}$ and this indicates that mild steel corroded locally in microscopic point of view.

• BMB Reports
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• 제37권4호
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• pp.408-415
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• 2004

The expression of the Paenibacillus sp. A11 cyclodextrinase (CDase) gene using the pUC 18 vector in Escherichia coli JM 109 resulted in the formation of an insoluble CDase protein in the cell debris in addition to a soluble CDase protein in the cytoplasm. Unlike the expression in Paenibacillus sp. A11, CDase was primarily observed in cytoplasm. However, by adding 0.5 M sorbitol as an osmolyte, the formation of insoluble CDase was prevented while a three-fold increase in cytoplasmic CDase activity was achieved after a 24 h-induction. The recombinant CDase protein was purified to approximately 14-fold with a 31% recovery to a specific activity of 141 units/mg protein by 40-60% ammonium sulfate precipitation, DEAE-Toyopearl 650 M, and Phenyl Sepharose CL-4B chromatography. It was homogeneous by non-denaturing and SDS-PAGE. The enzyme was a single polypeptide with a molecular weight of 80 kDa, as determined by gel filtration and SDS-PAGE. It showed the highest activity at pH 7.0 and $40^{\circ}C$. The catalytic efficiency ($k_{cat}/K_m$) values for $\alpha$-, $\beta$-, and $\gamma$-CD were $3.0{\times}10^5$, $8.8{\times}10^5$, and $5.5{\times}10^5\;M^{-1}\;min^{-1}$, respectively. The enzyme hydrolyzed CDs and linear maltooligosaccharides to yield maltose and glucose with less amounts of maltotriose and maltotetraose. The rates of hydrolysis for polysaccharides, soluble starch, and pullulan were very low. The cloned CDase was strongly inactivated by N-bromosuccinimide and diethylpyrocarbonate, but activated by dithiothreitol. A comparison of the biochemical properties of the CDases from Paenibacillus sp. A11 and E. coli transformant (pJK 555) indicates that they were almost identical.

• Geomechanics and Engineering
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• 제11권5호
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• pp.625-638
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• 2016

This paper presents field study and numerical modeling results for a single-cell low-fill concrete box culvert under a flexible pavement subjected to traffic loading. The culvert in the field test was instrumented with displacement transducers to capture the deformations resulting from different combinations of static and traffic loads. A low-boy truck with a known axle configuration and loads was used to apply seven static load combinations and traffic loads at different speeds. Deflections under the culvert roof were measured during loading. Soil and pavement samples were obtained by drilling operation on the test site. The properties of the soil and pavement layers were determined in the laboratory. A 3-D numerical model of the culvert was developed using a finite difference program FLAC3D. Linear elastic models were used for the pavement layers and soil. The numerical results with the material properties determined in the laboratory were compared with the field test results. The observed deflections in the field test were generally smaller under moving loads than static loads. The maximum deflections measured during the static and traffic loads were 0.6 mm and 0.41 mm respectively. The deflections computed by the numerical method were in good agreement with those observed in the field test. The deflection profiles obtained from the field test and the numerical simulation suggest that the traffic load acted more like a concentrated load distributed over a limited area on the culvert. Elastic models for culverts, pavement layers, and surrounding soil are appropriate for numerical modeling of box culverts under loading for load rating purposes.

• 한국전자통신학회논문지
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• 제13권4호
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• pp.695-704
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• 2018

X밴드 선박용 레이더를 이용하여 개발한 강우 추정 기술로 강우 사례를 검증하였다. 디지털 신호 변환기를 사용하여 선박용 레이더로부터 수신된 신호를 디지털 반사 정보로 변환하였다. 반사도 정보에서 지형클러터 신호를 제거하고, 빔 감쇠와 빔 부피의 변화에 의해 발생하는 오류를 보정 처리하였다. 처리된 반사 정보는 우량계 강우량과 선형적인 관계를 보였다. 레이더 신호를 직교좌표계로 변환하여 정량적 강우를 추정하였다. 자동기상관측소 세 지점에서 $5mm\;hr^{-1}$ 이상의 강우가 기록된 사례에서 선박용 레이더의 강우 분포는 기상청 운용 기상 레이더의 결과와 일치하였다.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.262-270
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• 2007

Of the twelve lytic bacteriophages recovered from five different fermenting cucumber tanks that were inoculated with Pediococcus sp. LA0281, a lytic phage, ${\phi}ps05$, was characterized in the present study. The plaques were mostly clear and round-shaped on the lawn of starter strain, indicating lytic phage. Overall appearance indicated that it belongs to the Siphoviridae family or Bradley's group B1, with a small isometric head and a flexible noncontractile tail with swollen base plate. The average size was found to be 51.2 nm in head diameter and 11.6 nm wide ${\times}$ 129.6 nm long for the tail. The single-step growth kinetics curve showed that the eclipse and the latent period were 29 min and 34 min, respectively, and an average burst size was calculated to be 12 particles per infective center. The optimum proliferating temperature ($35^{\circ}C$) was slightly lower than that of cell growth ($35\;to\;40^{\circ}C$). The structural proteins revealed by SDS-PAGE consisted of one main protein of 33 kDa and three minor proteins of 85, 58, and 52 kDa. The phage genome was a linear double-stranded DNA without cohesive ends. Based on the single and double digestion patterns obtained by EcoRI, HindIII, and SalI, the physical map was constructed. The overall size of the phage genome was estimated to be 24.1 kb. The present report describes the presence of a lytic phage active against a commercial starter culture Pediococcus sp. LA0281 in cucumber fermentation, and a preliminary study characterizes the phage on bacterial successions in the process of starter-added cucumber fermentation.