• 대한수의학회지
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• 제59권4호
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• pp.195-199
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• 2019

Disulfiram (DSF) is a member of the dithiocarbamate family that can bind copper. Recent studies have shown that DSF has anti-cancer activities, but the mechanism has not been clarified. Therefore, it is important to study the action mechanism of DSF to maximize its anticancer effects. A human leukemia cell line, HL-60, was used in this study. HL-60 cells were treated with DSF and the cellular metabolic activity was measured. DSF increased the cell death of HL-60 cells in annexin V-fluorescein isothiocyanate/propidium iodide staining analysis. In addition, DSF decreased the mitochondrial membrane potential (MMP) of the HL-60 cells. The cytotoxicity of DSF on HL-60 cells was observed at 0.4 μM. Interestingly, the reduction of MMP by DSF was recovered by N-acetyl-L-cysteine, an inhibitor of reactive oxygen species (ROS) production. This suggests that the decrease in MMP by DSF is closely related to the production of ROS in HL-60 cells, which indicates the relationship between the apoptosis of HL-60 cells by DSF and the role of the mitochondria. This study provides clinicians and researchers with valuable information regarding the anti-cancer activity of DSF in terms of the action mechanism.

• Archives of Pharmacal Research
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• 제21권5호
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• pp.610-614
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• 1998

DW-116, [1-(5-fluoro-2-pyridyl)-6-fluoro-7-(4-methyl-1-piperazinyl)-1,4-dihydro-4-oxoquino-line-3-carboxylic acid hydrochloride}, is a new quinolone antibiotic with a broad antibacterial spectrum against G(+) and G(-) bacteria. DW-116 was evaluated for the immunomodulating activities, which is one of the efforts to investigate the mechanism of action related to the good in vivo antibacterial efficacy. The results of in vitro studies revealed there was no statistically significant increase in B and T lymphocyte proliferation. But the results of in vivo studies showed that the number of plaque forming cells (PFC), the amount of polyclonal antibodies and delayed-type hypersensitivity (DTH) were significantly increased after the repeat administration with 12 and 60 mg/kg of DW-116. Taken together, these results proposed that immunostimulting effect of DW-116 could be one of the action mechanisms for demonstrating in vivo antibacterial activities under these experimental conditions.

• 대한한의학회지
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• 제39권4호
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• pp.158-170
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• 2018

Objectives: The objective of this study is Korean mistletoe lectin (Viscum album coloratum agglutinin, VCA) and bee venom (BV) to experimental prove comparative study of VCA and BV on the anti-cancer effect and mechanisms of action. Methods: In this study, it was examined in a human hepatocellular carcinoma cell line, Hep G2 cells. Cytotoxic effects of VCA and BV on Hep G2 cells were determined by 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay in vitro. VCA and BV killed Hep G2 cells in a time- and dose-dependent manner. Results: The apoptotic cell death was then confirmed by propidium iodide staining and DNA fragmentation analysis. The mechanisms of action was examined by the expression of anti-apoptotic proteins and activation of mitogen-activated protein kinases. Treatment of Hep G2 cells with VCA activated poly (ADP-ribose) polymerase-1 (PARP-1) known as a marker of apoptosis, and mitogen-activated protein kinases signaling pathways including SAPK/JNK, MAPK and p38. BV also activated PARP-1, MAPK, p38 but not JNK. The expression level of anti-apoptotic molecule, Bcl-X, was decreased by VCA treatment but not BV. Finally, the phosphorylation level of ERM proteins involved in the cytoskeleton homeostasis was decreased by both stimuli. Conclusion: We examined the involvement of kinase in VCA or BV - induced apoptosis by using kinase inhibitors. VCA-induced apoptosis was partially inhibited by in the presence.

• Journal of Electrical Engineering and Technology
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• 제12권5호
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• pp.1955-1962
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• 2017

Induction motors are a workhorse for the industry. The condition monitoring and fault analysis are the main concern for the engineers. The bearing is one of the vital segment of the induction machine and the condition of the whole machine is decided based on the condition of the bearing. In the present paper, the vibration signal of the bearing has been used for the analysis. The first line of action is to perform a statistical analysis of the vibration signal which gives trends in signal. To get the location of a fault in the bearing the second action is to develop an index based on Wavelet Packet Transform node energy named as Bearing Damage Index (BDI). Further, Teager-Kaiser Energy Operator (TKEO) has been calculated from higher index value to get the envelope and finally Power Spectral Density (PSD) has been applied to identify the fault frequencies. A performance index has also been developed to compare the usefulness of the proposed method with other existing methods. The result shows that the strong amplitude of fault characteristics and its side bands help to decide the type of fault present in the recorded signal obtained from the bearing.

• Food Science and Biotechnology
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• 제14권6호
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• pp.854-859
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• 2005

Although action modes of equol and genistein have been extensively studied, their precise roles in tumor cells remain elusive. To address possible effects of these compounds on protein expression in mammary tumor cells, proteins modulated in MCF-7 mammary tumor cells when incubated in absence and presence of 10 uM equol or genistein were identified through 2-dimensional gel electrophoresis, MALDI-TOF MS/MS, and NCBInr database search using Mascot software. Most proteins differentially expressed in MCF-7 cells after treatment with 10 uM genistein or equol were identified as being the same. Exposure to both compounds caused decreased cellular expression of RNA-binding protein regulatory subunit and oncogene DJ1 tubulin beta-1 chain, and increased expression of heterogeneous ribonucleoproteins F and L, KH-type splicing regulatory protein, and translation elongation factor EF-Tu precursor. Genistein and equol at dose used in this study showed common action mechanism.

• 한국자원식물학회지
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• 제33권3호
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• pp.183-193
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• 2020

Recently, as a natural substance has been emphasized interest in research to enhance the immune function. Green lettuce (Lactuca sativa L.) is a popular vegetable used fresh and it contains various phytochemicals and antioxidant compounds, and has been reported to have various physiological activities such as antibacterial, antioxidant, antitumor and anti-mutagenic. However, only a few studies have investigated on the mechanism of action of immune-enhancing activity of lettuce. Therefore, in this study, the immunomodulatory activities and potential mechanism of action of Green lettuce extracts (GLE) were evaluated in the murine macrophage cell line RAW264.7. GLE significantly increased NO levels by RAW264.7 cells, as well as expressions of immunomodulators such as iNOS, COX-2, IL-1β, IL-6, IL-12, TNF-α and MCP-1. Although GLE activated ERK1/2, p38, JNK and NF-κB, GLE-mediated expressions of immunomodulators was dependent on p38, JNK and NF-κB. In addition, TLR4 inhibition blocked GLE-mediated expressions of immunomodulators and activation of p38, JNK and NF-κB. Taken together, these results demonstrated that TLR4-MAPK/NF-κB signalling pathways participated in GLE-induced macrophage activation and GLE could be developed as a potential immunomodulating functional food.

• 전기학회논문지
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• 제56권11호
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• pp.2040-2050
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• 2007

In this study, we present a novel method for estimating the information of MU(motor unit) which is the basic element of human muscle by using surface EMG. Some of the method developed in this field could only estimate the numbers of MU that is activated. However, in our study the MU-simulator based on the line source model was designed to estimate the MU information including the numbers of MU and muscle fiber, conduction velocity, MU diameter, fiber diameter, and end plate position. The SMUAP(single motor unit action potential) detector was designed and CMAP(compound muscle action potential) by electrical stimulus was recorded. With these data, the MU-simulator can estimate the MU information by varying muscle paramater settings through MSE(mean square error) method. Our results shows that the proposed method can be comparable with the method of anatomical studies. Moreover, our system can be utilized to build a tool for diagnosis and treatment assessment of neuromuscular patients.

• 농촌계획
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• 제22권3호
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• pp.143-151
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• 2016

The purpose of this study is to introduce the village project for conservation and improvement of rural tourism village. So, in this study, the current rural tourism village and storytelling planning are examined and the village project presented in village planning is also examined. And this study propose the storytelling implementation of the village project through the case study related with the Susanri in Jeju. The results are summarized as follows. Firstly, it is important that each village projects are storytelling for tourism resource and improve rural tourism village. Secondly, it is necessary to define story range, action planning, and management agency of village projects. Thirdly, it is desirable that the village projects should be carried out as reviving the local community. Comparing to the storytelling tourism plan, there are few rural tourism plans so far, so, it is difficult to applicate the system of the village project, but, continuous studies in this line can help the rural tourism plan to ensure practicality.

• 대한전기학회논문지:전력기술부문A
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• 제51권6호
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• pp.262-268
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• 2002

Preventive control has to solve two important problems. The first is fast and accurate severity assessment of instability originated from the occurrence of a dangerous contingency. The second is to choose an action able to stabilize it. In this paper we assess contingencies in power systems using PASF(Power Angle Shape Filtering) and control power systems by a generation rescheduling. The control action stabilize the whole set of harmful contingencies simultaneously. Note that conventional time-domain transient stability methods can hardly tackle preventive control. So, we study the preventive control using off-line method. The proposed method is applied to prevent and to correct loss of synchronism of all the generators in a operating systems data.

• 조명전기설비학회논문지
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• 제12권2호
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• pp.21-28
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• 1998

본 연구는 산업계에서 많이 사용되고 잇는 3상 브리지 다이오드 정류기에서 입력측에 발생하는 고조파 문제를 개선하고자 한다. 다이오드 방식은 제어기가 필요치 않기 때문에 간단하고 높은 신뢰성을 갖지만 입력단 전류의 불연속성으로 인하여 전류파형의 왜곡이 생긴다. 본 연구에서는 기존의 3상 브리지 다이오드 정류기에 부가적으로 부착 가능한 3개의 스위치를 제안하여 매순간 3상의 전류 중에서 다이오드 정류기로 흐르지 못한 한 상의 전류를 제안한 스위치를 통하여 흐르게 하여 전류가 연속적으로 흐르게 하였다. 연속적인 전류의 흐름은 고조파 성분을 감소시키며 역률 또한 개선시키는 것을 모의 실험과 실험을 통하여 입증하였다.