• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.2819-2822
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• 2013

In this study, antiproliferative effects of the selective estrogen receptor modulator Tamoxifen and the aromatase inhibitor letrozole (Femara) were evaluated and compared using the FM3A cell line, originating from a C3H mouse mammary carcinoma and positive in terms of estrogen receptor (ER) expression. Cell kinetic parameters including labelling index, mitotic index and labelling index were assessed after exposure of the. FM3A cell line to $0.001{\mu}g/ml$ of Tamoxifen and $0.25{\mu}g/ml$ of Femara for 4, 8, 16 and 32 h for all parameters. The results showed that cell growth was inhibited by both agents. There was a significant decrease in labelling index and mitotic index and significant increase in apoptotic index for all experimental groups. The differences between control and all experimental groups were statistically significant (p<0.001) for all applications.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권6호
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• pp.461-467
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• 2005

Background. 5-(and-6)-carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester mixed (CFSE) is the fluorescent labelling agent of living cells and used to trace the cells in vivo after transplatnation of various cells. The CFSE labelled cells can maintain fluorescence for up to 7 days after labelling. The MC3T3-E1 cell line (MC3T3) has been used for many studies about osteoblast, which is well known as a mouse preosteoblast. So the CFSE would be used to trace the transplanted MC3T3. However there are few reports about CFSE labelling of MC3T3. This study is aimed to know about adequate concenturation and incubation time of CFSE to MC3T3. Materials and methods. The MC3T3 was incubated in a humidified atmosphere of 95% air with 5% $CO_2$ at $37^{\circ}C$ using ${\alpha}$-minimal essential medium (${alpha}$-MEM) containing10% FBS and gentamycin. Ten mM CFSE solution in dimethylsulphoxide (DMSO: 1%) was diluted with phosphate buffered saline (PBS) and final concentration of culture medium was, respectively, 5, 10, 15, 20, 25 and 30 ${{\mu}M$. Then the MC3T3 was incubated with CFSE in a humidified atmosphere of 95% air with 5% $CO_2$ at $37^{\circ}C$ for 5, 10, 15, 20, 25, 30, 35, 40 and 45 minutes in each concentration. The fluorescence of CFSE labelled cells was analysed with a inverted fluorescence microscope. The duration of cell labelling was also studied. Trypan blue dye exclusion test was done for cell viability. Results. For concentration between 5 and 10 ${\mu}M$, CFSE did not significantly label the MC3T3 in vitro. The destruction of MC3T3 was observed at the concentration of 20 ${\mu}M$. In the concentration of 15 ${\mu}M$, the best labelling was obtained at an incubation period between 15 and 30 minutes. The MC3T3 labelled with an incubation period of 15 minutes at 15 ${\mu}M$ was still fluorescent 7 days after CFSE labelling. The mean cell viability was 95.93%. Conclusion. These results suggests an incubation period of 15 minutes at 15 ${\mu}M$ of CFSE provides best labelling of MC3T3 in vitro.

• International Journal of CAD/CAM
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• 제5권1호
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• pp.51-58
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• 2005

Interpreting a natural line drawing as a solid object requires simplifying assumptions in order to make the problem more tractable. Unfortunately, some of the assumptions made in the past have overly simplified the problem. Restricting the valency of vertices, and in particular allowing only trihedral vertices, distorts the problem, since algorithms which are satisfactory for the simplified problem are not satisfactory in the general case. This paper presents a test set of drawings of objects with higher-valency vertices. The intention in creating this test set is that it may be used to determine how effective various algorithms are in dealing with general (i.e. unrestricted) valency vertices.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2351-2354
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• 2015

In this study, the antiproliferative effects of the metformin was evaluated on MCF-7 Cells (human breast adenocarcinoma cell line). For this purpose cell kinetic parameters including cell proliferation assay, mitotic index and labelling index analysis were used. $30{\mu}M$, $65{\mu}M$ and $130{\mu}M$ Metformin doses were applied to cells for 24, 48 and 72 hours. The results showed that there was a significant decrease in cell proliferation, mitotic index and labelling index for all experimental groups (p<0.05) for all applications.

• 한국정보처리학회논문지
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• 제7권2호
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• pp.558-568
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• 2000

본 논문에서는 등고선 지도의 3차원 정보를 이용하여 수치지형도 자동 생성방법을 제안한다. 제안된 방법은 등고선의 세선화와 라벨링을 통해 등고성분과 비 등고성분을 정확히 구분하였으며, Bezier 곡선을 사용하여 끊어진 등고선을 부드럽게 연결한다. 마지막으로 등고선으로부터 얻어진 고도 데이터를 사용하여 3차원 데이터를 생성한다. 제안된 방법은 수치 지형도를 만들기 위해 소요되는 막대한 양의 인력과 시간, 비용을 절약할 수 있다.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2004년도 춘계학술대회 논문집
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• pp.225-230
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• 2004

A cold ＆ hot rolling coil production line of iron nill consists of a kind of coherent automatic process, but an automatic labelling process still had technical difficulties in the automation of its process. The reason for difficulties in building an automatic process is that quantitative data for each rolled coil from every shipping is not easy to receive from the previous process. it is not possible to apply for a general and simple purpose robot that is actually worked through a taught position to the process because the size and direction of the coi1 has differed on every shipping. From these reasons. we introduce a robot vision system to accept an expected variable situation and to ensure the stability and flexibility of the process. This paper examines a study applied for similar cases and finds the position and direction of relied coil using the moment invariant algorithm proposed by Hu. In addition. the camera calibration and position error compensation algorithm is applied by the analysis of the relationship of transition in a space coordinate system. The construction of a robot vision system proposed by this paper is a more intellectual system than that of the automatic labelling system. which is already used to the Daihen steel nill of NEW JAPAN steel mill co. Ltd in Japan, and shows a better independent operation in the field of production.

• 한국정보통신학회논문지
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• 제19권11호
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• pp.2540-2545
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• 2015

본 논문에서는 복부 초음파 영상에서 충수를 추출하는 방법을 제안한다. 제안된 충수 추출 방법은 복부 초음파 영상에서 명암 대비를 강조하기 위해 Ends_in Search Stretching 기법을 적용하고, Max-Min 이진화, 영역 레이블링, 잡음제거, Cubic Spline 보간법을 적용하여 복부 근육의 하단 근막 부분을 추출한다. 그리고 초음파 영상에서 추출된 근막 영역을 제거한 후, ART2 알고리즘을 적용하여 충수를 추출한다. 제안된 방법을 초음파 영상에 적용한 결과, 제안된 충수 추출 방법이 효율적으로 충수가 추출되는 것을 영상의학과 전문의를 통하여 확인하였다.

• 대한치과보철학회지
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• 제33권2호
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• pp.317-334
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• 1995

The purpose of this study was to compare the bone formation, maturation around HA-and titaniumcoated dental implants in dog. 5 hydroxyapatite coated IMZ implants and 5 titanium coated IMZ implants were placed into the previously extracted site in the mandible of 5 adults dogs. All dogs were injected intravenously Tetracycline, Alizalin red S, and Calcein for bone fluorescent labelling, After the experimental period of 16weeks, the dogs were sacrificed and tissue samples around the implants were obtained. Microscopic observations(ligth, polariged and fluorescence microscope), morphometric analysis, line profile with EPMA, and quantitative analysis for Ca,P, and Ti were performed. The results were as follows ; 1. Bone maturations around the implants were relatively lower than those of natural teeth. No significant differences in bone maturation and remodeling patterns were observed between the two implants groups. 2. Calcification of bone surrounding the implants was initiated in 8-11 weeks for HA-coated implants, while it took 11 weeks or more for Ti-coated implants. 3. Bone-to-implants contact ratio of 82.63% was recorded for HA-coated group and 72.25% for titanium coated group, with no significant difference between the two groups. 4. Bone around the implants exhibited reduced quantity of Ca and P in the $100{\mu}m$ region relative to natural teeth, while the rest of the regions showed no statistical differences. No significant differences were found between the two implant groups. 5. There was a separation of HA layer from the implant core and subsequent infiltration of inflammatory cells into the resulting space in the HA-coated implants, and evidences of phagocytosis of HA particles by macrophages. Bone calcification was more rapid around HA-coated implants compared to titanium-coated implants, but HA coated implants did not show any significant differences either in the degree of calcification or the bone-to-implant contact ratio over Ti coated implants. HA coated implants may have complications associated with HA absorption and separation of HA layer from the implant core.

• 한국응용곤충학회지
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• 제22권1호
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• pp.41-44
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• 1983

본 실험은 내충성품종을 선별하는 데 방사성동위원소인 P-32의 이용성을 검토하기 위해서 흰등멸구에 대해 기왕에 저항성 및 감수성으로 판정되어 있는 10개 품종을 선별하여 섭식량과 식이선호성간의 관계를 조사중 다음과 같은 결과를 얻었다. 1) 약충의 식이선호도와 P-32 섭식량간에는 밀접한 관계가 있었다. 2. 수칭유묘를 표식할 때는 근부와 엽의 길이 및 수를 일정하게 한 후 침적시키는 것이 P-32의 균일성을 인정할 수 있었다. 3. 내충성 검정시 공시충은 성충을 이용하는 것이 약충을 이용하는 것 보다 품종간에 상대적인 비교가 용이하였다.

• 조명전기설비학회논문지
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• 제21권1호
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• pp.19-27
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• 2007

본 논문에서는 모폴로지 기법을 이용한 TFT-LCD 셀의 라인 결함과 픽셀 결함을 검사할 수 있는 알고리즘을 개발하였다. 이때 LCD 셀의 브라이트 라인 결함, 다크 라인 결함, 브라이트 픽셀 결함, 다크 픽셀 결함들을 검출하기 위하여, 셀의 크기 특성을 고려한 모폴로지 연산자의 모양을 결정하고, 팽창 연산, 침식 연산 및 차분 기법을 이용하여 결함 정보를 추출하였다. 이후 다양한 실험을 통하여 결정된 적절한 임계값을 이용한 최적의 이진화 알고리즘을 적용하였다. 마지막으로 결함정보의 인식을 위한 라벨링 과정을 통하여, 결함들을 검출하였다. TFT-LCD 판넬의 다양한 검사 실험을 통하여, 본 논문에서 제안하는 알고리즘의 결함정보 검출 성능이 매우 우수함을 확인하였다.