• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2819-2822
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• 2013

In this study, antiproliferative effects of the selective estrogen receptor modulator Tamoxifen and the aromatase inhibitor letrozole (Femara) were evaluated and compared using the FM3A cell line, originating from a C3H mouse mammary carcinoma and positive in terms of estrogen receptor (ER) expression. Cell kinetic parameters including labelling index, mitotic index and labelling index were assessed after exposure of the. FM3A cell line to $0.001{\mu}g/ml$ of Tamoxifen and $0.25{\mu}g/ml$ of Femara for 4, 8, 16 and 32 h for all parameters. The results showed that cell growth was inhibited by both agents. There was a significant decrease in labelling index and mitotic index and significant increase in apoptotic index for all experimental groups. The differences between control and all experimental groups were statistically significant (p<0.001) for all applications.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.31 no.6
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• pp.461-467
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• 2005

Background. 5-(and-6)-carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester mixed (CFSE) is the fluorescent labelling agent of living cells and used to trace the cells in vivo after transplatnation of various cells. The CFSE labelled cells can maintain fluorescence for up to 7 days after labelling. The MC3T3-E1 cell line (MC3T3) has been used for many studies about osteoblast, which is well known as a mouse preosteoblast. So the CFSE would be used to trace the transplanted MC3T3. However there are few reports about CFSE labelling of MC3T3. This study is aimed to know about adequate concenturation and incubation time of CFSE to MC3T3. Materials and methods. The MC3T3 was incubated in a humidified atmosphere of 95% air with 5% $CO_2$ at $37^{\circ}C$ using ${\alpha}$-minimal essential medium (${alpha}$-MEM) containing10% FBS and gentamycin. Ten mM CFSE solution in dimethylsulphoxide (DMSO: 1%) was diluted with phosphate buffered saline (PBS) and final concentration of culture medium was, respectively, 5, 10, 15, 20, 25 and 30 ${{\mu}M$. Then the MC3T3 was incubated with CFSE in a humidified atmosphere of 95% air with 5% $CO_2$ at $37^{\circ}C$ for 5, 10, 15, 20, 25, 30, 35, 40 and 45 minutes in each concentration. The fluorescence of CFSE labelled cells was analysed with a inverted fluorescence microscope. The duration of cell labelling was also studied. Trypan blue dye exclusion test was done for cell viability. Results. For concentration between 5 and 10 ${\mu}M$, CFSE did not significantly label the MC3T3 in vitro. The destruction of MC3T3 was observed at the concentration of 20 ${\mu}M$. In the concentration of 15 ${\mu}M$, the best labelling was obtained at an incubation period between 15 and 30 minutes. The MC3T3 labelled with an incubation period of 15 minutes at 15 ${\mu}M$ was still fluorescent 7 days after CFSE labelling. The mean cell viability was 95.93%. Conclusion. These results suggests an incubation period of 15 minutes at 15 ${\mu}M$ of CFSE provides best labelling of MC3T3 in vitro.

• International Journal of CAD/CAM
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• v.5 no.1
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• pp.51-58
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• 2005

Interpreting a natural line drawing as a solid object requires simplifying assumptions in order to make the problem more tractable. Unfortunately, some of the assumptions made in the past have overly simplified the problem. Restricting the valency of vertices, and in particular allowing only trihedral vertices, distorts the problem, since algorithms which are satisfactory for the simplified problem are not satisfactory in the general case. This paper presents a test set of drawings of objects with higher-valency vertices. The intention in creating this test set is that it may be used to determine how effective various algorithms are in dealing with general (i.e. unrestricted) valency vertices.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2351-2354
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• 2015

In this study, the antiproliferative effects of the metformin was evaluated on MCF-7 Cells (human breast adenocarcinoma cell line). For this purpose cell kinetic parameters including cell proliferation assay, mitotic index and labelling index analysis were used. $30{\mu}M$, $65{\mu}M$ and $130{\mu}M$ Metformin doses were applied to cells for 24, 48 and 72 hours. The results showed that there was a significant decrease in cell proliferation, mitotic index and labelling index for all experimental groups (p<0.05) for all applications.

• The Transactions of the Korea Information Processing Society
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• v.7 no.2
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• pp.558-568
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• 2000

In the paper, we propose the automatic generation method of digital elevation map based on contour map. The proposed method classifies contour data and non-contour data by thinning and labelling process of the contour line and then connects smoothly broken contour line by Bezier curve. Finally, the digital devation map is generated by the interpolation using the height data of the contour line. The proposed method can reduce vest effort, time and expense which is spend to make digital elevation map.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2004.04a
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• pp.225-230
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• 2004

A cold ＆ hot rolling coil production line of iron nill consists of a kind of coherent automatic process, but an automatic labelling process still had technical difficulties in the automation of its process. The reason for difficulties in building an automatic process is that quantitative data for each rolled coil from every shipping is not easy to receive from the previous process. it is not possible to apply for a general and simple purpose robot that is actually worked through a taught position to the process because the size and direction of the coi1 has differed on every shipping. From these reasons. we introduce a robot vision system to accept an expected variable situation and to ensure the stability and flexibility of the process. This paper examines a study applied for similar cases and finds the position and direction of relied coil using the moment invariant algorithm proposed by Hu. In addition. the camera calibration and position error compensation algorithm is applied by the analysis of the relationship of transition in a space coordinate system. The construction of a robot vision system proposed by this paper is a more intellectual system than that of the automatic labelling system. which is already used to the Daihen steel nill of NEW JAPAN steel mill co. Ltd in Japan, and shows a better independent operation in the field of production.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.11
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• pp.2540-2545
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• 2015

In this paper, we propose a novel method to extract appendix from ultrasound image automatically to avoid such subjectivity issue. In the process, we apply a series of image processing algorithms such as Ends_in search stretching for emphasizing brightness contrast and binarization, region labelling, and cubic spline interpolation for extracting lower bound fasicia line that is the base of extracting the appendix. Knowing that the appendix is located at the lower organ area below the bottom fascia line, we conduct a series of image processing techniques to find the fascia line correctly. And then we apply ART2 algorithm to the organ area in order to extract appendix accurately. Through experiment, the effectiveness of the proposed method is verified based on the field experts' evaluations.

• The Journal of Korean Academy of Prosthodontics
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• v.33 no.2
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• pp.317-334
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• 1995

The purpose of this study was to compare the bone formation, maturation around HA-and titaniumcoated dental implants in dog. 5 hydroxyapatite coated IMZ implants and 5 titanium coated IMZ implants were placed into the previously extracted site in the mandible of 5 adults dogs. All dogs were injected intravenously Tetracycline, Alizalin red S, and Calcein for bone fluorescent labelling, After the experimental period of 16weeks, the dogs were sacrificed and tissue samples around the implants were obtained. Microscopic observations(ligth, polariged and fluorescence microscope), morphometric analysis, line profile with EPMA, and quantitative analysis for Ca,P, and Ti were performed. The results were as follows ; 1. Bone maturations around the implants were relatively lower than those of natural teeth. No significant differences in bone maturation and remodeling patterns were observed between the two implants groups. 2. Calcification of bone surrounding the implants was initiated in 8-11 weeks for HA-coated implants, while it took 11 weeks or more for Ti-coated implants. 3. Bone-to-implants contact ratio of 82.63% was recorded for HA-coated group and 72.25% for titanium coated group, with no significant difference between the two groups. 4. Bone around the implants exhibited reduced quantity of Ca and P in the $100{\mu}m$ region relative to natural teeth, while the rest of the regions showed no statistical differences. No significant differences were found between the two implant groups. 5. There was a separation of HA layer from the implant core and subsequent infiltration of inflammatory cells into the resulting space in the HA-coated implants, and evidences of phagocytosis of HA particles by macrophages. Bone calcification was more rapid around HA-coated implants compared to titanium-coated implants, but HA coated implants did not show any significant differences either in the degree of calcification or the bone-to-implant contact ratio over Ti coated implants. HA coated implants may have complications associated with HA absorption and separation of HA layer from the implant core.

• Korean journal of applied entomology
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• v.22 no.1 s.54
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• pp.41-44
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• 1983

In order to evaluate the technique of P-32 labelling method for screening lines of rice to whitebacked planthopper, the relationship between the amount of ingestion and feeding preference of insects were observed with the resistant and susceptible lines where characteristics were predetermined by comparing their feeding preference and antibiosis method. The feeding preference of the insects was significantly correlated with the amount of P-32 ingestion. It was more manifest in adults than nymphs of the whitebacked planthopper. The density of nymph and radioactivity of P-32 were high in the susceptible line. This suggested that deforming the ingestion amount of P-32 in the insects seemed to be useful technique for accurate screening. For this technique, the uniform labelling of P-32 on the rice seedings was prerequisite and the uniformity was increased by triming roots and leaves in the length and number.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.21 no.1
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• pp.19-27
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• 2007

In this paper, we develope and implement a TFT-LCD cell defects detection algorithm using morphology. To detect the bright line or dark line defects and the bright pixel or dark pixel defects of the TFT-LCD cells, we determine the shape of the morphology operators considering the shape characteristics of the TFT-LCD sub pixels. Using dilation, erosion, and the subtraction operators, we extract gray level defects information. Then, we apply the optimal threshold method which shows the best results in terms of several criteria. Finally, we determine the defects using labelling method. From various experiments using TFT-LCD panels, the proposed algorithm shows superior results.