• 한국정밀공학회지
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• 제16권1호통권94호
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• pp.42-48
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• 1999

A control method for a robot hand grasping a object in a partially unknown environment will be proposed, where a proximate sensor detecting the distance between the fingertip and object was used. Particularly, the finger joints were driven servo-pneumatically in this study. Based on the proximate sensor signal the finger motion controller could plan the grasping process divided in three phases ; fast aproach, slow transitional contact and contact force control. That is, the fingertip approached to the object with full speed, until the output signal of the proximate sensor began to change. Within the perating range of the proximate sensor, the finger joint was moved by a state-variable feedback position controller in order to obtain a smooth contact with the object. The contact force of fingertip was then controlled using the blocked-line pressure sensitivity of the flow control servovalve for finger joint control. In this way, the grasping impact could be reduced without reducing the object approaching speed. The performance of the proposed grasping method was experimentally compared with that of a open loop-controlled one.

• BMB Reports
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• 제44권3호
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• pp.211-216
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• 2011

Vitamin C (VC) is an important antioxidant and enzyme co-factor that works by stimulating the immune system and protecting against infections. It is well known that melanoma cells are more susceptible to VC than any other tumor cells. However, the role of VC in the treatment of colon cancer has not been studied. Cisplatin (CDDP) is a DNA damaging agent and is widely used for treating cancer, while the role of p53 in CDDP-induced cell death has been stressed. Using cell growth assays, morphological methods, Western blotting, flow cytometry, and DNA fragmentation analysis, we measured the expression of p53 level involved in the effect of VC on CDDP-induced apoptosis of HCT116, a human colon cancer cell line. CDDP plus VC treatment resulted in significantly increased apoptosis along with upregulation of p53 compared to untreated cells and/or CDDP-treated cells. These results suggest that VC enhanced CDDP sensitivity and apoptosis via upregulation of p53.

• 대한수의학회지
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• 제58권1호
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• pp.27-31
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• 2018

Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between $1.97{\times}10^4/mL$ and $9.85{\times}10^3/mL$ for samples with a 2-fold serial dilution from $1.25{\times}10^6\;TCID_{50}$ ($TCID_{50}$, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 하계학술대회 논문집 A
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• pp.609-611
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• 2003

This paper presents a method for a congestion management in the competitive market which is modeled as the multiple bilateral transaction. Traditionary, the congestion management for the multiple bilateral transaction is accomplished through the process of adjustment bids. It is necessary in for the market entity to submit their price information when the congestion is occured. Finally, the ISOs can be blown about the approximated contract price of participants from the price of adjustment bids. However, the entities can submit only the amount of their contract to ISOs and ISOs are required the method to manage the congestion only by the contracted quantity. Therefore, this paper presents a method for congestion management by curtailing the only contracted quantity of market entities. To evaluated the above problem, we suggest Bilateral Transaction Distribution Factors(BTDFs), which is the sensitivity of line flow with curtailment of transactions. Using this factor, we studied about congestion management when the objective function is to minimize total curtailment of transaction.

• 대한전기학회논문지:전력기술부문A
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• 제55권5호
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• pp.202-213
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• 2006

This paper presents a new approach for optimal dispatch of energy and frequency regulation reserve considering contingency in a competitive electricity market. It is necessary to introduce the reserve market with the spot energy market for operating efficiently and obtaining the security of the electricity market. However, the reserve market is closely connected with the energy market since the energy and reserve are produced from the same resources. Thus, it is inevitable to co-optimize the energy and ancillary service for efficient operation of energy and ancillary service market. Therefore, this paper proposes a new method for optimal dispatch of energy and frequency regulation reserve considering n-1 contingency of generator and transmission line using constraints and sensitivity based on AC power flow To verify the effectiveness of the proposed method, the numerical studies have been performed for 5-bus sample system and modified IEEE 14-bus system.

• 한국산학기술학회논문지
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• 제19권2호
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• pp.689-694
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• 2018

최근 친환경 자동차 개발과 함께 대기 오염 문제 대응을 위해서 자동차 연비 개선은 차량 개발에 있어서 매우 중요하다. 개발 차량의 연비 향상을 위한 요소 중 차량 외관을 타고 흐르는 공기 흐름과 차량 후부에서의 공기 유동 현상은 중요한 요소이다. 특별히 SUV(Sports Utility Vehicle) 차량의 차량 후부 공기 유동은 리어 스포일러 설계 시 고려해야 하는 중요한 설계 항목 중 하나이다. 본 연구에서는 차량의 공기 유동에 영향을 주는 리어 스포일러의 제어 인자를 정하고 각 제어 인자의 공기 유동 특성에 대한 민감도를 시험을 통하여 비교 분석하고, 유동 저항력을 감소시킬 수 있는 스포일러의 최적 제어 인자를 결정하였다. 결정된 최적 제어 인자를 이용한 시험 모델을 만들어 시험평가하고 그 결과 값에 대한 S/N비와 평균값을 분석하여 제어 인자 값을 변경하였다. 변경된 최적 제어 인자 값을 이용한 개선 모델을 다시 제작하고 유동 시험을 실시하여 공기 저항력 감소 효과와 원가절감 효과를 확인하였다.

• 농업과학연구
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• 제39권4호
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• pp.613-618
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• 2012

This study was performed to optimize the basic platform of a lateral flow immunoassay. Improvement of the limit of detection (LOD) was evaluated according to the width of a nitrocellulose membrane with varying concentrations of analyte. The analyte, neutravidin was detected based on the avidin-biotin interaction. The antibody-Au nanoparticle conjugation was mostly stabled in a PBS buffer of pH 7.3. The optimal widths of a nitrocellulose membrane were 4 and 6 mm considering the sample flow rate and signal strength of the test line on the membrane. The LOD of neutravidin was 0.001 mg/ml in the optimum conditions.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.6115-6120
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• 2013

Introduction: Some non-small cell lung cancer (NSCLC) tumor cells are insensitive to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) -based therapy. This study was conducted to examine the effect of embelin on the sensitivity of the A549 NSCLC cell line to TRAIL receptor2 (TRAILR2) monoclonal antibodies and to investigate the potential mechanisms. Materials and Methods: A549 cells were treated with embelin, TRAILR2 mAb or a combination of both. Cell viability was measured using ATPlite assay and apoptosis rates were determined by flow cytometry with AnnexinV-FITC and propidium iodide staining, with the expression levels of proteins analyzed by Western blotting. Results: The cell survival rate of separate treatments with 100 ng/ml TRAILR2 antibody or 25 uM embelin were $81.5{\pm}1.57%$ and $61.7{\pm}2.84%$, respectively. Their combined use markedly decreased cell viability in A549 cells to $28.1{\pm}1.97%$ (P<0.05). The general caspase inhibitor Z-VAD-FMK could inhibit the embelin-enhanced sensitivity of A549 cells to TRAILR2 mAb ($75.97{\pm}3.17%$)(P<0.05). Both flow cytometry and cell morphological analysis showed that embelin was able to increase TRAIL-induced apoptosis in A549 cells. Combined treatment with embelin and TRAILR2 mAb augmented the activation of initiator caspases and effector caspase. In addition, A549 cells showed increasing levels of TRAILR2 protein and decreasing levels of Bcl-2, survivin and c-FLIP following the treatment with embelin+TRAILR2 mAb. Conclusions: Embelin could enhance TRAIL-induced apoptosis in A549 cells. The synergistic effect of the combination treatment might be due to modulation of multiple components in the TRAIL receptor-mediated apoptotic signaling pathway, including TRAILR2, XIAP, survivin, Bcl-2 and c-FLIP.

• 한국전자파학회논문지
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• 제29권7호
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• pp.477-483
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• 2018

본 논문에서는 FM 방송대역(88~108 MHz)에서 동작하는 자동차 전면 글래스 상에 설계되는 투명 안테나를 제안한다. 투명 안테나는 전형적으로 차량 지붕에 설치된 샤크핀 안테나의 공간적 제한을 피하기 위해 차량 전면 글래스에 구현하였다. 전면 글래스에 설계되는 안테나는 낮은 수심감도와 다른 서비스대역의 안테나로부터의 방사간섭 문제를 합리적으로 해결할 수 있다. 전면 글래스는 고유한 closed-line 구조를 가지고 있으며, 이 구조로 인해 전면 글래스 외곽의 차체 표면으로 전류가 흐르게 되어 FM 방송대역 이전 부분에서 첫 공진점이 발생한다. Closed-line 구조를 이용한 표면 전류 길이 제어를 통해 첫 공진점이 FM 대역에서 동작하는 안테나를 설계하였다. 또한, RF 수동소자로 설계하기 적합한 투명전극 소자 마이크로 메탈메쉬 필름의 투명성을 통해 시각적 인식의 최소화 하였다. 측정된 안테나는 FM 대역에서 반사계수는 -6 dB 이하이며, average peak gain은 -0.9 dB이다. 본 연구를 통해 제안된 전면 글래스 투명안테나는 미래의 자동차 안테나 개발에 필요한 공간과 설계 자유에 대한 가능성을 보여주었다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.6135-6140
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• 2013

Background: Breast cancer is a common malignant tumor which affects health of women and multidrug resistance (MDR) is one of the main factors leading to failure of chemotherapy. This study was conducted to establish paclitaxel-resistant breast cancer cell line and nude mice models to explore underlying mechanisms of MDR. Methods: The breast cancer drug-sensitive cell line MCF-7 (MCF-7/S) was exposed in stepwise escalating paclitaxel (TAX) to induce a resistant cell line MCF-7/TAX. Cell sensitivity to drugs and growth curves were measured by MTT assay. Changes of cell morphology and ultrastructure were examined by optical and electron microscopy. The cell cycle distribution was determined by flow cytometry. Furthermore, expression of proteins related to breast cancer occurrence and MDR was tested by immunocytochemistry. In Vivo, nude mice were injected with MCF-7/S and MCF-7/TAX cells and weights and tumor sizes were observed after paclitaxel treatment. In addition, proteins involved breast cancer and MDR were detected by immunohistochemistry. Results: Compared to MCF-7/S, MCF-7/TAX cells had a higher resistance to paclitaxel, cross-resistance and prolonged doubling time. Moreover, MCF-7/TAX showed obvious alterations of ultrastructure. Estrogen receptor (ER) expression was low in drug resistant cells and tumors while expression of human epidermal growth factor receptor 2 (HER2) and Ki-67 was up-regulated. P-glycoprotein (P-gp), lung resistance-related protein (LRP) and glutathione-S-transferase-${\pi}$ (GST-${\pi}$) involved in the MDR phenotype of resistant cells and tumors were all overexpressed. Conclusion: The underlying MDR mechanism of breast cancer may involve increased expression of P-gp, LRP and GST-${\pi}$.