• Title/Summary/Keyword: Limit of experimental method

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A new nano-composite carbon ink for disposable dopamine biosensors (나노컴포지트 카본 잉크가 전착된 일회용 도파민 바이오센서)

  • Dinakaran, T.;Chang, S.-C.
    • Analytical Science and Technology
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    • v.29 no.1
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    • pp.35-42
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    • 2016
  • A new nano-composite carbon ink for the development of disposable dopamine (DA) biosensors based on screen-printed carbon electrodes (SPCEs) is introduced. The method developed uses SPCEs coupled with a tyrosinase modified nano-composite carbon ink. The ink was prepared by an “in-house” procedure with reduced graphene oxide (rGO), Pt nanoparticles (PtNP), and carbon materials such as carbon black and graphite. The rGO-PtNP carbon composite ink was used to print the working electrodes of the SPCEs and the reference counter electrodes were printed by using a commercial Ag/AgCl ink. After the construction of nano-composite SPCEs, tyrosinase was immobilized onto the working electrodes by using a biocompatible matrix, chitosan. The composite of nano-materials was characterized by X-ray photoelectron spectroscopy (XPS) and the performance characteristics of the sensors were evaluated by using voltammetric and amperometric techniques. The cyclic voltammetry results indicated that the sensors prepared with the rGO-PtNP-carbon composite ink revealed a significant improvement in electro-catalytic activity to DA compared with the results obtained from bare or only PtNP embedded carbon inks. Optimum experimental parameters such as pH and operating potential were evaluated and calibration curves for dopamine were constructed with the results obtained from a series of amperometric detections at −0.1 V vs. Ag/AgCl. The limit of detection was found to be 14 nM in a linear range of 10 nM to 100 µM of DA, and the sensor’s sensitivity was calculated to be 0.4 µAµM−1cm−2.

Transparent Manipulators Accomplished with RGB-D Sensor, AR Marker, and Color Correction Algorithm (RGB-D 센서, AR 마커, 색수정 알고리즘을 활용한 매니퓰레이터 투명화)

  • Kim, Dong Yeop;Kim, Young Jee;Son, Hyunsik;Hwang, Jung-Hoon
    • The Journal of Korea Robotics Society
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    • v.15 no.3
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    • pp.293-300
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    • 2020
  • The purpose of our sensor system is to transparentize the large hydraulic manipulators of a six-ton dual arm excavator from the operator camera view. Almost 40% of the camera view is blocked by the manipulators. In other words, the operator loses 40% of visual information which might be useful for many manipulator control scenarios such as clearing debris on a disaster site. The proposed method is based on a 3D reconstruction technology. By overlaying the camera image from front top of the cabin with the point cloud data from RGB-D (red, green, blue and depth) cameras placed at the outer side of each manipulator, the manipulator-free camera image can be obtained. Two additional algorithms are proposed to further enhance the productivity of dual arm excavators. First, a color correction algorithm is proposed to cope with the different color distribution of the RGB and RGB-D sensors used on the system. Also, the edge overlay algorithm is proposed. Although the manipulators often limit the operator's view, the visual feedback of the manipulator's configurations or states may be useful to the operator. Thus, the overlay algorithm is proposed to show the edge of the manipulators on the camera image. The experimental results show that the proposed transparentization algorithm helps the operator get information about the environment and objects around the excavator.

Spectrofluorimetric Determination of Pb ion in Aqueous Media Using an Optical Sensor (광센서를 이용한 수용액 중 납이온의 형광분광법적 정량)

  • Lee, Sang Hak;Seo, Hyo Suk
    • Journal of the Korean Chemical Society
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    • v.46 no.5
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    • pp.407-411
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    • 2002
  • A method to determine lead ion in aqueous media using an optical sensor loaded on a fluorescent optode membrane incoporating a metal ion-selective ionophore, a proton-selective chromoionophore and lipophilic anionic sites has been studied. The effects of pH and thickness of membrane on the fluorescence intensity were investigated. The effects of foreign ions such as $Na^+$, $K^+$, $Mn^{2+}$ and $Zn^{2+}$ on the determination of lead ion were also studied. The linear range in the calibration curve for the determination of lead ion was found to be 5.0${\times}10^-7$ to 5.0${\times}$$10^-3$M and the correlation coefficient in this range was -0.99107 under the optimal experimental conditions. The relative standard deviation of the blank signals was 3.0% and the detection limit of lead ion was 5.0${\times}$$10^-9$M.

The improved facial expression recognition algorithm for detecting abnormal symptoms in infants and young children (영유아 이상징후 감지를 위한 표정 인식 알고리즘 개선)

  • Kim, Yun-Su;Lee, Su-In;Seok, Jong-Won
    • Journal of IKEEE
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    • v.25 no.3
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    • pp.430-436
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    • 2021
  • The non-contact body temperature measurement system is one of the key factors, which is manage febrile diseases in mass facilities using optical and thermal imaging cameras. Conventional systems can only be used for simple body temperature measurement in the face area, because it is used only a deep learning-based face detection algorithm. So, there is a limit to detecting abnormal symptoms of the infants and young children, who have difficulty expressing their opinions. This paper proposes an improved facial expression recognition algorithm for detecting abnormal symptoms in infants and young children. The proposed method uses an object detection model to detect infants and young children in an image, then It acquires the coordinates of the eyes, nose, and mouth, which are key elements of facial expression recognition. Finally, facial expression recognition is performed by applying a selective sharpening filter based on the obtained coordinates. According to the experimental results, the proposed algorithm improved by 2.52%, 1.12%, and 2.29%, respectively, for the three expressions of neutral, happy, and sad in the UTK dataset.

Elasto-Plastic Behavior of Steel Beams with High Strength Bolted Splices (고력볼트 접합이음 철골보의 탄소성거동)

  • Choi, Sung Mo;Kim, Jin Ho;Roh, Won Kyoung
    • Journal of Korean Society of Steel Construction
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    • v.15 no.5 s.66
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    • pp.531-539
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    • 2003
  • Unlike field-welded moment frames damaged during the Northridge earthquake, a column-tree moment frame has a tool to control and reduce its seismic behavior. The tool is the girder splice. Girder splices could be designed to be sufficiently ductile and to have a prescribed bending moment capacity. In such a design, during earthquakes, the girder splices would act as ductile "fuses" and limit the magnitude of forces including the bending moment that could be developed in the frame. In Korea, most moment frames arc composed of a column-tree moment frame. Therefore, the elasto-plastic behavior of steel beams with high strength bolted friction splice should be clarified. Furthermore, structural capacities, including energy absorption capacity, must be quantitatively found. This paper discusses an experimental study to clarify elasto-plastic behavior of steel beams with high strength bolted friction splices. A total of 5 specimens were tested. A specimen was fabricated to have a beam splice designed by a full strength method. Other specimens were fabricated to have beam splices with 75%, 50% and 0% capacities compared with the specimen.

A Research of Grain Size Analysis of Particulate Matter in Fire Effluent (연소 생성물 내 입자상 물질의 입도에 관한 연구)

  • Kim, Sung-Soo;Choi, Seo-Yeon;Rie, Dong-Ho
    • Fire Science and Engineering
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    • v.28 no.6
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    • pp.8-12
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    • 2014
  • Interior finishing materials using noncombustible were regulated by the building codes to prevent the spread of fire and protect occupants. The average deed of stopping time of experimental mouse exposing combustion gas were measured by KS F 2271 gas toxicity test. At that time, The average deed of stopping time under 9 minutes were judged a inconsistence. This experiment method has limit to find out a cause of toxicity effect factor. In this study, particle size analysis were performed for investigate a major factor.

An Analytical Evaluation on Buckling Resistance of Tapered H-Section Deep Beam (춤이 큰 웨브 변단면 H형 보의 휨내력에 대한 해석적 평가)

  • Lee, Seong Hui;Shim, Hyun Ju;Lee, Eun Taik;Hong, Soon Jo;Choi, Sung Mo
    • Journal of Korean Society of Steel Construction
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    • v.19 no.5
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    • pp.493-501
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    • 2007
  • Recently, in the domestic amount of materials,curtailment and economic efficiency security by purpose, tapered beam application is achieved, but the architectural design technology of today based on the material non-linear method does not consider solutions to problems such as brittle fracture. So, geometric non-linear evaluation thatincludes initial deformation, width-thickness ratio, web stiffener and unbraced length is required. Therefore, in this study, we used ANSYS, a proven finite elementanalysis program,and material and geometric non-linear analysis to study existing and completed tapered H-section as deep beam's analysis model. Main parameters include the width-thickness ratio of web, stiffener, and flange brace, with the experimental result obtained by main variable buckling and limit strength evaluation. We made certain that a large width-thickness ratio of the web decreases the buckling strength and short unbraced web significantly improves ductility.

Fuel-rich Combustion with AP added Propellant in a Staged Hybrid Rocket Engine (다단 하이브리드 로켓에서 AP 첨가 추진제의 연료과농 연소)

  • Lee, Dongeun;Lee, Changjin
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.44 no.7
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    • pp.576-584
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    • 2016
  • In this study, AP added propellant has been proposed as a method of enhancing the low specific impulse performance found for staged hybrid rocket engine. Experimental tests were carried out to analyze and evaluate the effect of AP added propellant on specific impulse performance as well as fuel-rich combustion characteristics in a staged hybrid rocket engine. Upper limit of AP content in propellant was set to be 15 wt% to maintain the hybrid rocket engine advantages. As a result, 15 wt% AP added propellant showed 3% higher specific impulse performance compared to 0 wt% AP added propellant. Moreover, AP addition proved to offer less injected oxidizer mass flow, less O/F variation, and less combustion pressure while producing fuel-rich gas of the same combustion temperature. Future studies will carry out more combustion tests with metal additives to further enhance specific impulse.

A Study on eye-tracking software design and development for e-sports viewing on the web (e 스포츠 웹 시청 연구를 위한 시선 분석도구 설계 및 개발)

  • Ko, Eunji;Choi, SunYoung
    • Journal of Korea Game Society
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    • v.15 no.4
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    • pp.121-132
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    • 2015
  • This study suggests a design for an analytical software program and method for multitasking e-sports viewing through the web using an eye tracking device. To fulfill this task, we designed a Window of Interest (WOI) to measure and record visually on a screen wherever numerous multitasking activities occur. In addition, we developed an OBS (Opensource Broadcaster Software) plug-in that records and streams participant viewing behavior patterns in real time. The purpose of this study is as follows. First, unlike existing tools that limit web interface recording to still images, the developed tool can record dynamically via media such as videos. Second, when several windows are processed on a screen, the tool can accurately record the gaze positions of the participants. Lastly, the tool can enhance the objective validity of the data as it can be implemented in natural situations. Therefore, this study can trace natural viewing patterns and behavior as we do not create artificial experimental environments and stimuli.

Establishment of Choline Analysis in Infant Formulas and Follow-up Formulas by Ion Chromatograph (이온크로마토그래프를 이용한 조제유류 및 영아용·성장기용 조제식 중 콜린 함량 분석법 연구)

  • Hwang, Kyung Mi;Ham, Hyeon Suk;Lee, Hwa Jung;Kang, Yoon Jung;Yoon, Hae Seong;Hong, Jin Hwan;Lee, Hyoun Young;Kim, Cheon Hoe;Oh, Keum Soon
    • Journal of Food Hygiene and Safety
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    • v.32 no.5
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    • pp.411-417
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    • 2017
  • This study was conducted to establish the analysis method for the contents of choline in infant formulas and follow-up formulas by ion chromatograph (IC). To optimize the method, we compared several conditions for extraction, purification and instrumental measurement using spiked samples and certified reference material (CRM; NIST SRM 1849a) as test materials. IC method for choline was established using Ion Pac CG column and 18 mM $H_2SO_4$ mobile phase. The parameters of validation were specificity, linearity, LOD, LOQ, recovery, accuracy, precision and repeatability. The specificity was confirmed by the retention time and the linearity, $R_2$ was over 0.999 in range of 0.5~10 mg/L. The detection limit and quantification limit were 0.14, 0.43 mg/L. The accuracy and precision of this method using CRM were 95%, 2.1% respectively. Optimized methods were applied in sample analysis to verify the reliability. All the tested products were acceptable contents of choline compared with component specification for nutrition labeling. The standard operating procedures were prepared for choline to provide experimental information and to strengthen the management of nutrient in infant formula and follow-up formula.