• Journal of Plant Biotechnology
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• 제45권2호
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• pp.146-153
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• 2018

Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. Previous studies on Lilium tissue culture have been linked to direct regeneration and a few studies have been conducted on indirect regeneration, which has been studied under bright conditions. In this study, for the first time in the world, all the stages of indirect regeneration (callus induction, shoot and root induction) have been studied under dark conditions. Callus formation and the regeneration levels of L. Ledebourii Bioss were examined for three replicates in an MS (Murashige and Skoog) medium with different hormonal compositions and by using a factorial experiment in the framework of a completely random plan. For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In terms of callus wet weight, the highest amount was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In addition, in terms of diameter, the highest amount was found in $3{\mu}M$ of 2,4-D, and $0.5{\mu}M$ of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of $3{\mu}M$ of 2,4-D, and $0.1{\mu}M$ of kinetin. This study contended that auxin and cytokinin can induce long shoots and roots through cell elongation in dark condition.

• Journal of Plant Biotechnology
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• 제6권1호
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• pp.9-13
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• 2004

Transient uidA expression was used to optimize parameters required for biolistic transformation of suspension cells of Easter lily, Lilium longiflourm. Maximum uidA expression occurred following bombardment with gold particles as compared to tungsten. A 3hr pre-treatment of suspension cells with 0.125M osmoticum resulted in a 1.5X increase in uidA expression. A helium pressure of 1550 psi combined with a particle travelling distance of 6cm resulted in maximum uidA expression as compared to either 1100, 1200, or 1800 psi. Transient transformation resulted in up to 493 uidA expressing cells/Petri plate. For stable transformation suspension cells of Lilium longiflorum, were co-bombarded with plasmid DNA containing cucumber mosaic virus (CMV) replicase under the rice actin (Act1) promoter and either the bar or PAT genes under the cauliflower mosaic virus (CaMV 355) promoter. Ten regenerated plants contained the transgene as analyzed by PCR, and two of the ten plants were confirmed to contain the transgene by Southern hybridization. The two transgenic plants were independent transformants, one containing the bar gene and the other both the CMV replicase and bar genes. Plants were sprayed at the rosette stage and found to be resistant to 1000 mg/L of phosphinothricin (Trade name-Ignite) indicating expression of the bar gene throughout the leaves when bar was under control of the CaMV 35S promoter.

• 식물조직배양학회지
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• 제28권6호
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• pp.341-346
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• 2001

본 실험에서 Lilium oriental hybrid 'Casa Blanca'. asiatic hybrid 'Mona'와 longiflorum hybrid 'Hinomoto'의 기내 인편으로부터의 자구 형성 및 비대를 위해 MS 배지 내 무기물 농도를 0.5∼2배로, 배지 내 총 질소 농도를 30∼120 mM로 달리하여 자구 형성과 생장을 조사한 결과 인편으로부터의 자구 형성수는 0.5와 1배의 MS 배지 무기물 농도 또는 30 mM의 총 질소 농도에서 'Mona' 품종이 다른 품종에 비해 가장 많았다. 'Casa Blanca'와 'Hinomoto'에서는 0.5배 MS 무기물 농도 또는 30 mM의 질소 농도가 자구 형성에 가장 효과적이었다. 자구 비대는 2배 MS 무기물 농도 또는 120 mM 총질소 농도에서 3품종 모두 양호하였다.

• 원예과학기술지
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• 제19권4호
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• pp.591-595
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• 2001

'Casablanca'와 'Marco Polo' 품종의 구근 저장중 발생하는 부패병을 방제하기 위해 azoxystrobin, benomyl+thiram 및 prochloraz와 길항세균배양액 등을 처리하여 적정 약제 및 농도를 구명하고, 약제처리된 구근의 생육상 및 절화의 품질을 조사하여 약해의 유무를 판별하고자 수행하였다. 백합구근이 부패병 발생은 azoxystrobin과 prochloraz가 억제 효과가 있으며, Benomyl+thiram는 국내산 구근의 부패병 발병 억제 효과가 없는 것으로 조사되었다. 생리적으로 백합구근의 견고성을 증가시키기 위해 처리한 calcium chloride 혼용처리는 부패병을 증가시켰다. 길항세균 배양액을 1/5로 희석하여 처리하였을 때, 부패병이 발생을 효과적으로 억제하였다. 재배중 'Casablanca' 품종은 benomyl+thiram 처리구에서, 'Marco Polo' 품종은 benomyl+thiram와 calcium 혼용 처리구에서 맹아 억제가 심하였다. 구근의 저장전 약제처리는 백합의 영양생장 과정에서 생육상과 꽃의 품질에는 영향을 주지 않으며, calcium과 benomyl+thiram 처리구에서 잎과 꽃의 길이가 다른 처리구에 비해 작으나 유의차는 없었다.

• 원예과학기술지
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• 제19권4호
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• pp.585-590
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• 2001

본 연구는 강원도 평창의 고랭지에서 1998년 5월부터 10월까지 백합의 생육 향상과 우량구근 생산을 위해 여러 가지 피복재를 재배에 이용하였다. 피복처리재는 톱밥, 흑색필름, 반사필름, 투명필름, 그리고 백흑이중필름을 사용하였다. 백합 공시 품종은 일본 수출에 유리한 'Casablanca'와 'Marco Polo'를 재식하였다. 흑색필름과 백흑이중필름 피복재배는 백합 잎의 생장과 발육을 지연시켰다. 피복재배는 'Casablanca' 품종의 지상부 생체중을 증가시켰다. 구근생장은 흑색필름과 백흑이중필름 피복구를 제외한 처리구는 무처리구와 유사한 구근 생육상을 보여주었다. 톱밥과 반사필름 피복재배가 무처리구와 다른 피복처리구보다 백합 지상부 생육, 지하부 생육, 그리고 구근생육에 유리하였다. 흑색필름은 지상부와 지하부 생육, 그리고 개화 향상에 적합하지 않은 것으로 조사되었다. 투명필름처리와 백흑이중필름은 백합재배 시 무처리구와 비교하여 지상부생육과 구근 생산에 뚜렷한 증진 효과가 없었다. 한편 피복재가 대체적으로 생육을 지연시키는 효과가 있어 관행수확기보다 수확기를 다소 늦추는 것이 백합 구근생산에 유리할 것으로 판단된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.43-43
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• 2020

Plants regenerated from in vitro cultures are associated with chromosomal variations, which have been generally found in long-term culture. Reducing plant culture age is one of the ways to reduce genetic and epigenetic changes. The present study focused on the efficient in vitro propagation of lily cultivars and has intensified to speed up bulb propagation for cryopreservation. The multiplication process applied in this experiment uses starting material, which the newly small bulb formed from bulb-scales in two lily cultivars. The adventitious bulb from bulb-scale tissue cultured on three different media following Murashige and Skoog (MS) basal medium supplemented with 1 g/L Charcoal, MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone with or without Charcoal, respectively. After about seven weeks, there is little change in the number of newly propagated bulbs in small bulbs of the two media. Compared to the both mediums, the number of the propagated bulbs is increased 5 times in MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone without Charcoal. After about seven weeks, the results of the propagation showed that the number of the propagated bulbs is increased 5 times in MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone without Charcoal compared to the both mediums. The number of propagated bulbs ranged from 5 to 6 and 4 to 6 with an average of 5 in Tropicalpink and Greenstar cultivars, respectively. There is little change in the number of newly propagated bulbs in small bulbs of other media. The multiplication process applied in this study may save in vitro culture period and effort.

• 한국육종학회지
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• 제41권4호
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• pp.583-586
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• 2009

"Hyehwa" an Asiatic lily cultivar was released in 1998 at National Institute of Horticultural and Herbal Science (RDA), Suwon, Korea. The cross was made in 1991 between Asiatic lily "White Bird", a white colored, and "Avignon", an unspotted scarlet red colored. It was preliminarily selected as A93-20 in 1993. Its multiplication, bulbing growth and flowering characteristic tests were conducted from 1994 to 1998. A new cultivar "Hyehwa" flowers in middle of June and grows 98.7 cm in height. Flowers bloom upward-facing, thick orange (RHS, 28A). Year-round flowering is possible by storage of the bulb under $-1.5^{\circ}C$ conditions. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. Botrytis disease control is needed in the wet season.

• 한국육종학회지
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• 제40권4호
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• pp.546-549
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• 2008

'Yeeun' an Asiatic hybrid lily was released in 2005 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Suwon, Korea. The cross was made in 1994 between Liliium Asiatic 'White Bird' white colored, and Liliium Asiatic 'Cote d'Azur', pink colored variety. It was preliminarily selected as 'A96-5' in 1996. Multiplication and bulbing, and characteristic tests were conducted from 1997 to 2004. The evaluation of characteristics was investigated as 'Wongyo C1-21' in 2004 at Suwon. 'Yeeun' flowers in the end of June and grows 41.6 cm stem length. Flowers are upward-facing, unspotted in petals and yellow green (RHS, 18C). Year-round flowering can be by storing the bulb under $-1.5^{\circ}C$ conditions. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. It is needed to control Botrytis disease in wet season.

• Current Research on Agriculture and Life Sciences
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• 제9권
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• pp.147-155
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• 1991

노지재배(露地栽培)한 Lilium elegans 'Connecticut king'의 구근(球根) 굴취시기(掘取時期)를 개화기(開花期)인 6월 5일부터 7월 25일까지 10일(日) 간격(間隔)으로 나누고 잔존엽수(殘存葉數)를 달리해서 일정기간(一定期間) 냉장처리(冷藏處理)($3{\pm}1^{\circ}C$, 0~105일간(日間) 8처리(處理))한 후(後) 촉성재배(促成栽培)한 결과(結果)는 다음과 같다. 1. 맹아율(萌芽率)은 저온처리기간(低溫處理期間)이 길어질수록, 잔존엽수(殘存葉數)가 많을수록 그리고 구근비대기간(球根肥大期間)이 길수록 증가(增加)하는 경향(傾向)이었으며 개화기(開花期)의 미숙구근(未熟球根)도 짧은 저온처리(低溫處理)로 높은 맹아율(萌芽率)을 나타내었다. 개화율(開花率)을 보면 개화기(開花期)의 미숙구근(未熟球根)과 엽(葉) 10매(枚)를 남기고 50일간(日間) 비대(肥大)시킨 구근(球根) 그리고 엽(葉)20매(枚)를 남기고 30일이상(日以上) 비대(肥大)시킨 구근(球根)을 90일간(日間) 저온처리(低溫處理)하였을때, 또 전엽(全葉)을 남기고 30일이상(日以上) 비대(肥大)시킨 구근(球根)을 60일간(日間) 저온처리(低溫處理)했을때 90%이상(以上)의 높은 개화율(開花率)을 나타내었다. 2. 맹아(萌芽) 및 개화(開花)소요일수(日數)는 저온처리일수(低溫處理日數)가 증가(增加)함에 따라 짧아지는 경향(傾向)이었으며 특히 개화(開花)소요일수(日數)에 있에서는 개화기(開花期)의 미숙구근(未熟球根)이 타(他) 처리구(處理區)에 비해 가장 짧았고 정식(定植) 후(後) 100 일 이내에 개화(開花)시킬 수 있었다. 3. 초장(草長), 착화수(着花數), 경경(莖經)등은 공히 개화후(開花後) 구근비대기간(球根肥大期間)이 길수록 증가(增加)하였으며 개화기의 미숙구근(未熟球根)이 가장 저조한 생육(生育)을 나타내었다. 그러나 대구(大球)를 사용(使用)한다면 구근비대기간(球根肥大期間)없이 개화기(開花期)의 미숙구근(未熟球根)을 사용(使用)하여 연내(年內) 절화재배(切花栽培)가 가능할 것으로 판단(判斷)되었다.

• 한국자원식물학회지
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• 제34권1호
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• pp.17-22
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• 2021

Plants regenerated from in vitro cultures carry chromosomal variations, especially in long-term culture. Reducing the duration of plant tissue culture is one of the ways to reduce genetic and epigenetic changes. In this study, we reduced the duration of long-term culture and repeat subculture using small bulblets derived from bulb scales in two lily cultivars. The adventitious bulblets derived from bulb-scale tissue were cultured on three different media containing Murashige and Skoog (MS) basal medium supplemented with 1 g/L Charcoal, MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone with or without Charcoal, respectively. About seven weeks later, the number of newly propagated multiple shoots in the two media, A and B media, showed little differentiation. Compared to both media, the number of propagated multiple shoots increased 5-fold in MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone without Charcoal (C medium). The number of propagated multiple shoots ranged from 5 to 6 and 4 to 6 with an average of 5 in TropicalPink and GreenStar cultivars, respectively. The flow cytometric measurements indicated no variation in the ploidy level between control and in vitro propagated plants.