• Title/Summary/Keyword: Lilium

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Development of CAPS marker for identifying a Formosan lily (Lilium formosanum) (흰나리(Lilium formosanum Wallace) 식별을 위한 CAPS 마커의 개발)

  • Chung, Sung Jin;Lee, Ka Youn;Yoon, A Ra;Jang, Ji Young;Kim, Jin Kug;Lee, Geung-Joo
    • Korean Journal of Agricultural Science
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    • v.41 no.2
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    • pp.101-106
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    • 2014
  • This study was conducted to identify lily species native to Korea from formosan lily (Lilium formosanum) belonging to Longiflorum section. Due to flowering time, flower color and orientation, long shelf life and resistant to diseases, the native lily species can be valuable genetic resources for interspecific hybrids. One of the chloroplast genes, matK, was used to clone and sequence to explore any base changes. The matK was successfully amplified into 1,539 bp (94% of the gene) and phylogenetic tree demonstrated 6 clades for those 11 lily species used in this study. There were one or two base substitutions among 10 lilies native to Korea, while formosan lily native to Taiwan exhibited 6 base substitutions in matK gene, rendering it genetically distant. A restriction enzyme NruI recognized one of the six base changes, and digested the matK gene of 10 native lily species only, but not in formosan lily. The confirmed cleavage characteristic of the target region in matK gene was designed into a CAPS (cleaved amplified polymorphic sequences) marker which will be available to estimate compatibility of interspecific hybridization and to trace the pedigree when those native lilies are crossed with the formosan lily.

Analysis on Characteristics of Distribution of Specific Plants in Juwangsan National Park, Korea (주왕산국립공원의 특정식물 분포 특성분석)

  • You, Ju-Han;Seo, Jung-Keun;Jung, Sung-Gwan
    • Journal of Environmental Science International
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    • v.22 no.7
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    • pp.873-884
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    • 2013
  • This study was carried out to establish the conservation plan of specific plants by surveying and analyse the individuals and the environment of habitats on specific plants designated by Juwangsan national park office. The specific plants were 14 species including Berchemia berchemiaefolia, Hylotelephium ussuriense, Aristolochia manshuriensis, Lilium cernuum, Thalictrum coreanum, Exochorda serratifolia, Lilium distichum, Aristolochia contorta, Jeffersonia dubia, Eleutherococcus senticosus, Salix chaenomeloids, Eranthis stellata, Scopolia japonica, and Paeonia japonica. The habitats of specific plants were 40 sites including Berchemia berchemiaefolia of 11 sites, Hylotelephium ussuriense of 8 sites, Lilium cernuum and Thalictrum coreanum of each 3 sites, Exochorda serratifolia of 2 sites, and Lilium distichum, Aristolochia contorta, Jeffersonia dubia, Eleutherococcus senticosus, Salix chaenomeloids, Eranthis stellata, Scopolia japonica and Paeonia japonica of each 1 site. The altitude was the most in 300~400 m. In direction, NW was the most, and it showed SW in that order. The slope angle was the most in 0~10%, and the cliff showed in that order. In the results of species composition by stratum layer, Berchemia berchemiaefolia had the most, 15 species, in tree layer. In shrub and herb layer, this had the most as 20 species and 29 species, too. The species that occupied the largest size was Salix chaenomeloids in 2,500 $m^2$, and Jeffersonia dubia showed 1,500 $m^2$ in that order. The conservation of specific plants is to accomplish the ex situ and in situ conservation at the same time.

Lilium longiflorum 'Charm' as a F1 Hybrid for Pot Plant (종자번식 일대잡종 분화용 나팔나리(Lilium longiflorum) 'Charm' 육성)

  • Song, Cheon Young
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.304-308
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    • 2008
  • Lilium longiflorum 'Charm' as a $F_1$ hybrid cultivar was released by crossing inbred line '$L_2$-14' and '$L_2$-21' which were obtained from 5 self crosses originated from 'Nellie White', 'Ace' and 'Hinomoto'. The growth and flowering characteristics were evaluated in a greenhouse maintained at a minimum of $13^{\circ}C$ at night during winter in 2006 and 2007. The flower of 'Charm' is white color and horizontal-facing. The flower number of a plant and its diameter is 7.4 and 16.5 cm with 24.5 ornamental(flowering) days. The plant height is 60.3 cm with 70.3 number of leaves. The stem diameter and internode length is 1.2 cm and 1.1 cm, respectively, meaning the plant is compact and sturdy. And the number of seed per a capsule is 251.1. The results of these evaluation, therefore, suggest that seedling Lilium longiflorum 'Charm' can be used as a pot plant due to its short stems, many number of flowers per plant, long ornamental period, strong growth habit with many leaves and thick stem diameter.

Transgenic Plants of Easter Lily (Lilium longiflorum) with Phosphinothricin Resistance

  • Ahn, Byung Joon;Joung, Young Hee;Kamo, Kathryn K.
    • Journal of Plant Biotechnology
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    • v.6 no.1
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    • pp.9-13
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    • 2004
  • Transient uidA expression was used to optimize parameters required for biolistic transformation of suspension cells of Easter lily, Lilium longiflourm. Maximum uidA expression occurred following bombardment with gold particles as compared to tungsten. A 3hr pre-treatment of suspension cells with 0.125M osmoticum resulted in a 1.5X increase in uidA expression. A helium pressure of 1550 psi combined with a particle travelling distance of 6cm resulted in maximum uidA expression as compared to either 1100, 1200, or 1800 psi. Transient transformation resulted in up to 493 uidA expressing cells/Petri plate. For stable transformation suspension cells of Lilium longiflorum, were co-bombarded with plasmid DNA containing cucumber mosaic virus (CMV) replicase under the rice actin (Act1) promoter and either the bar or PAT genes under the cauliflower mosaic virus (CaMV 355) promoter. Ten regenerated plants contained the transgene as analyzed by PCR, and two of the ten plants were confirmed to contain the transgene by Southern hybridization. The two transgenic plants were independent transformants, one containing the bar gene and the other both the CMV replicase and bar genes. Plants were sprayed at the rosette stage and found to be resistant to 1000 mg/L of phosphinothricin (Trade name-Ignite) indicating expression of the bar gene throughout the leaves when bar was under control of the CaMV 35S promoter.

Effect of MS Medium Strength and Nitrogen Concentration on Bulblet Formation and Growth of Lilium In Vitro (MS 배지 내 무기물 농도 및 질소 농도가 나리의 자구 형성과 비대에 미치는 영향)

  • 염미란;박현춘;양찬석;백기엽
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.6
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    • pp.341-346
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    • 2001
  • To investigate the effects of MS medium strength and nitrogen concentration on bulblet formation from bulblet scale segment culture and bulblet growth from bulblet of Lilium oriental hybrid 'Casa Blanca', asiatic hybrid 'Mona', and longiflorum hybrid 'Hinomoto', 0.5∼2.0 strength of MS salts and 30∼120 mM nitrogen concentrations of MS medium were examined in vitro. The number of bulblets from bulb scale segment was favored in the strength of 0.5∼1.0 strength of MS salts or 30 mM total nitrogen concentration of MS medium in three cultivars. But the growth of bulblets formed in vitro was promoted in the 2 strength of MS medium or hight concentration of total nitrogen of MS medium up to 120 mM in three experimented cultivars.

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Conservation of An Endangered Species of Lilium cernum Komarvo. through in vitro Mass-Propagation (멸종위기 종 솔나리의 기내 대량생산을 통한 보존 연구)

  • Lee, Song-Hee;Lee, Su-Gwang;Kang, Ho-Duck
    • Korean Journal of Medicinal Crop Science
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    • v.19 no.1
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    • pp.9-15
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    • 2011
  • This study was conducted to establish the optimal condition for conservation of genetic resources and micropropagation of Lilium cernum. Induction of bulbet of L. cernum was highly effective (9.2 bulb/explant) on 1/2 SH (Schenk and Hildebrandt) medium supplemented with 1.0 mg/L TDZ (Thidiazuron) and 0.1 mg/L NAA (Naphthaleneacetic acid). The treatment of 0.1 mg/L NAA increased root development (6.4 root/explant) under the in vitro condition. In addition, treatments of AC (Activated Charcoal) and ventilation were enhanced to develop number of shoots and to elongate length of leaf, bulb and root. Futhermore, the process of short-term soil acclimatization was promoted to strengthen the plantlets induced under the in vitro condition.

Effects of sucrose and MS salts in the addition of liquid medium on in vitro bulblet growth of Lilium oriental hybrid 'Casa Blanca' (액체배지 첨가시 sucrose 및 MS 염류가 Lilium Oriental Hybrid 'Casa Blanca' 의 기내 자구비대에 미치는 영향)

  • 한봉희;예병우;백기엽;염미란
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.5
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    • pp.239-242
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    • 2001
  • The effects of post-cultural addition of liquid medium addition to stimulate in vitro bulblet growth in Lilium oriental hybrid 'Casa Blanca' were investigated. The sections of bulblets with swollen basal plate (7 mm$\times$12 mm) were cultured on medium containing 60 g/L sucrose and 1 g/L activated charcoal for two months in dark, and then, liquid medium was added into the same vessels. The addition of liquid medium stimulated the growth of bulblets remarkably, compared to no addition of liquid medium. The liquid medium supplemented with 120 g/L sucrose and double strength of MS salts were the most effective on the growth of in vitro bulblets.

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Detection and Molecular Characterization of a Stolbur Phytoplasma in Lilium Oriental Hybrids

  • Chung, Bong-Nam;Jeong, Myeong-Il
    • The Plant Pathology Journal
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    • v.19 no.2
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    • pp.106-110
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    • 2003
  • Stolbur Phytoplasma was detected from Lilium Oriental hybrids showing flattened stem and flower clustering. The presence of phytoplasma was demonstrated using polymerase chain reaction(PCR) assays with phyto-plasma-universal(P1/P6)and stolbur phytoplasma-specific 16F1/R1-S primer pairs amplifying phytoplasma 16S rDNA regions. Nucleotide suquences of the phytoplasma 16S rDNA were determined. Nucleic acid extracted from lily amplified 1.5 kb DNA with a phytoplasma universal primer pair. In nested PCR, 1.1 kb PCR product was obtained using specific primer pair, indicating an isolate of stolbur phytoplasma. Nucleotide sequence of phytoplasma 16S rDNA reported in this study showed 99.5% and 99.1% identities with two known stolbur phytoplamas (16Sr XII-A). Also, it exhibited a sequence homology of 98.0% with phormium yellow leaf (16Sr XII-B), and 97.9% with Australian grapevine yellows (16Sr XII-B). Meanwhile, it showed 98.1% identity with strawberry green petal phytoplama, (16Sr1-C), and 94.7 % with American aster yellows (16Sr1-B). Homology percentage of the 16S rDNA nucleotide sequence suggests that this phytoplama could be classified into the stolbur phytoplasma, subgroup A (16Sr XII-A), as a type strain stolbur.

De novo transcriptome sequencing and gene expression profiling with/without B-chromosome plants of Lilium amabile

  • Park, Doori;Kim, Jong-Hwa;Kim, Nam-Soo
    • Genomics & Informatics
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    • v.17 no.3
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    • pp.27.1-27.9
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    • 2019
  • Supernumerary B chromosomes were found in Lilium amabile (2n = 2x = 24), an endemic Korean lily that grows in the wild throughout the Korean Peninsula. The extra B chromosomes do not affect the host-plant morphology; therefore, whole transcriptome analysis was performed in 0B and 1B plants to identify differentially expressed genes. A total of 154,810 transcripts were obtained from over 10 Gbp data by de novo assembly. By mapping the raw reads to the de novo transcripts, we identified 7,852 differentially expressed genes (log2FC > |10|), in which 4,059 and 3,794 were up-and down-regulated, respectively, in 1B plants compared to 0B plants. Functional enrichment analysis revealed that various differentially expressed genes were involved in cellular processes including the cell cycle, chromosome breakage and repair, and microtubule formation; all of which may be related to the occurrence and maintenance of B chromosomes. Our data provide insight into transcriptomic changes and evolution of plant B chromosomes and deliver an informative database for future study of B chromosome transcriptomes in the Korean lily.

Ecogeographical variations of the vegetative and floral traits of Lilium amabile Palibian

  • Nguyen, Viet Yen;Rai, Rameshwar;Kim, Jong-Hwa;Kim, Ji-Young;Na, Jong-Kuk
    • Journal of Plant Biotechnology
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    • v.48 no.4
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    • pp.236-245
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    • 2021
  • This study aimed to characterize the morphological variations in the vegetative and floral traits of 73 wild Lilium amabile plants from six habitats in Korea. It was observed that L. amabile is distributed nationwide at any altitude from 300 m (Mt Mangdaeam) to 1550 m (Mt Halla). The majority of the natural habitats of L. amabile were found on mountain slopes, and some were found in rugged mountain regions. The down-facing flowers of this species not only had many blotches but also dense trichomes, and the flowering time was found to be from mid-June to mid-July. ANOVA revealed significant variations in vegetative and floral traits among the six habitats, indicating that the environment has substantial influences on the various growth parameters of L. amabile, such as plant height; number of leaves, bracts, papillae, and flowers; leaf angle; and lengths of the anther, longest blotch, and nectary of the petiole. In addition, the vegetative and floral traits were found closely correlated with each other under the direct impact of the environment. These findings will facilitate to find the appropriate environmental conditions for the conservation and development of L. amabile population as future lily-breeding materials.