• Korean Journal of Agricultural and Forest Meteorology
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• v.10 no.3
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• pp.75-81
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• 2008

This study was conducted to analyze the influence of simulated acid rain on leaves of Liliodendron tulipifera seedlings. The seedlings were treated with four levels of simulated acid rain with pH of 5.6, 4.9, 3.9 and 2.9, and then chlorophyll contents and the degree of foliar damage were investigated. Differences were statistically significant among treatments in chlorophyll contents in all of the tested soils. The total contents of chlorophyll tended to decrease as the simulated acid rain treatment was maintained. Chlorophyll contents, however, did not decrease according to the decrement of pH levels. Foliar damage increased as the simulated acid rain was maintained in all tested soils. Especially, the damage dramatically increased between May and July as the acidity increased. As the treatment of simulated acid rain continued, deformed new leaves appeared. In all treatments except the control, the leaves turned brown and the damage increased with time. Necrotic spots appeared during the first month of treatment at the pH level of 2.9 in all soil types. The damage by acid rain should be considered when we plant Liliodendron tulipifera.

• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.204-214
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• 2008

This study was conducted to analyze the influence of simulated acid rain on growth of Liliodendron tulipifera seedlings. The seedlings were treated with four levels of simulated acid rain, 5.6, 4.9, 3.9, or 2.9, and dry weight, growth of stem and root were investigated. There were statistically significant differences at 1% and 5% in the total dry weight of the above-part among the simulated acid rain treated groups with different levels of pH and different types of soil. The dry weight of the above part tended to decrease as the acidity of the simulated acid rain increases. The total dry weight of the below-part was significantly different according to the levels of acidity of the acid rain in all three soils and was shown a significant difference according to the soil types at only pH 2.9 plot. The dry weight of the below part in soils A and C decreased as the pH level decreases. The rate of stem growth was significantly different among the treatment groups of acidity of the acid rain at significance level of 0.01 and among the treatment groups of soil types at 0.01 and 0.05 levels from June to August. In all three soil types, the greatest stem growth occurred during the period of June. Moreover, stem growth was promoted at pH 3.9 plot and pH 4.9 plot whereas it was suppressed at pH 2.9 plot. Though the amount of fine roots and very fine roots in soil depth of 0-7 cm and 7-14 cm were significantly different among the treatment groups of pH level, fine root was not shown a significant difference among the pH groups in soil depth of 14-21cm. The types of soil significantly affected only on the amount of the very fine root.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.343-356
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• 2010

Plant micropropagation techniques include bud cultures using apical or axillary buds, organogenesis through callus culture or adventitious bud induction, and somatic embryogenesis. In Korea Forest Research Institute (KFRI), the first tissue culture trial in woody plant was initiated from the bud culture of hybrid poplars (Populus alba x P. glandulosa) in 1978. Since then several mass propagation techniques have developed from conifer and hardwood species, resulting in allowing practical application to Poplars, Birches and some oak species. In addition, useful micropropagation and genetic resources conservation techniques were established in some rare and endangered tree species including Abeliophyllum distichum. Among various in vitro propagation techniques, somatic embryogenesis is known to be the most efficient plant regeneration system. Since the first somatic embryo induction was reported in Tilia amurensis by KFRI in 1986, various protocols for direct or indirect somatic embryogenesis systems have developed in conifer and hardwood species including Larix leptolepis, Pinus rigida x P. taeda F1, Kalopanax septemlobus and Liliodendron tulipifera, etc. However, most of these technologies have been developed using juvenile tissues, i.e. immature zygotic embryos or mature embryos. Therefore it has been difficult to directly application to tree breeding program due to their unproven genetic background. Recently remarkable progresses and new approaches have been achieved in mature tree somatic embryogenesis. In this article we reviewed several micropropagation techniques, which have been mainly developed by KFRI and recent international progresses.

• Korean Journal of Agricultural and Forest Meteorology
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• v.19 no.4
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• pp.280-292
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• 2017

Hail is not a frequently occurring weather event, and there are even fewer reports of hail damages to forest stands. Since the 2000s, an increase in hail incidence has been documented in Europe and the United States. In Korea, severe hails occurred in Jeollanam-do province on May 31 and in Gyeongsangbuk-do province on June 1, 2017. Hail size was ranged from 0.5 to 5.0 cm in diameter in Jeollanam-do, and from 1.5 to 3.0 cm in Gyeongsangbuk-do. This study was aimed to analyze the hail damages to forests by species and topography based on damage-categorized maps created by using drones and aerial photographs, and to analyze relationships of the damages with meteorological factors. The total damaged forest area was 1,163.1ha in Jeollanam-do, and 2,942.3ha in Gyeongsangbuk-do. Among the 'severe' damaged area 326.7ha, 91% was distributed in Jeollanam-do, and concentrated in the city of Hwasun which covers 57.2% of the total 'severe' damaged area. The most heavily damaged species was Korean red pine(Pinus densiflora S. & Z.) followed by P. rigida. Most broad-leaved trees species including oaks were recovered without any dead trees found. Liliodendron tulipifera was the most severely damaged in terms of the rate of 'severe' degree individuals which are needed to be checked whether they will die or be recovered. Cause of the death of pines was considered as the combination of physical damage caused by the hail and long-lasting drought with high air temperature that occurred before and after the hail event. No pathogens and insects were found which might have affected to tree deaths. We suggested a dieback mechanism of the pine trees damaged by hail and drought.