The movement of teeth during orthodontic treatment requires bone remodeling process in periodontal tissue. To find out the changes occuring in the cell itself, mechanical force was applied to the cultured periodontal ligament cells. Following results were obtained from measuring the changes in cyclic AMP and $PGE_2$, $^3H$-thymidine incorporation amount in time lapse after application of mechanical force. 1. When mechanical force was applied to cultured PDL cells, the amount of cAMP in cells were increased significantly after 15 min. of force application, but were decreased gradually as time lapsed. 2. When mechanical force was applied to cultured PDL cells, the amount of PGE2 were increased at 20,40,60 min. and was significantly increased at 20 min. 3. When mechanical force was applied to cultured PDL cells, the amount of $^3H$-thymidine incorporation was some increased, but was not statistically significant.
In this research, experimental and numerical simulations were adopted to investigate the effects of ligament angle on compressive strength and failure mode of rock-like material specimens containing two non-coplanar filled fissures under uniaxial compression. The experimental results show that with the increase of ligament angle, the compressive strength decreases to a nadir at the ligament angle of $60^{\circ}$, before increasing to the maximum at the ligament angle of $120^{\circ}$, while the elastic modulus is not obviously related to the ligament angle. The shear coalescence type easily occurred when ${\alpha}$ < ${\beta}$, although having the same degree difference between the angle of ligament and fissure. Numerical simulations using $PFC^{2D}$ were performed for flawed specimens under uniaxial compression, and the results are in good consistency with the experimental results. By analyzing the crack evolution process and parallel bond force field of rock-like material specimen containing two non-coplanar filled fissures, we can conclude that the coalescence and propagation of crack are mainly derived from parallel bond force, and the crack initiation and propagation also affect the distribution of parallel bond force. Finally, the displacement vectors in ligament region were used to identify the type of coalescence, and the results coincided with that obtained by analyzing parallel bond force field. These experimental and numerical results are expected to improve the understanding of the mechanism of flawed rock engineering structures.
Vascular changes in the periodontal ligament of the rat incisors following application of experimental orthodontic forces were examined by the India ink perfusion method. 57 rats were used for this experiment. The rats were divided into experimental group (54 rats) and control group (3 rats). 54 experimental rats were divided into group I (27 rats) and group II (27 rats). The right and left upper incisors of group. I and group II rats were separated distally with forces of 20gm, 70gm respectively. The vascular changes of periodontal ligament were observed histologically by means of light microscope after 1, 2 and 3 days of tooth movement and 1,3,5,8,14, and 21 days after removal of orthodontic force. The results were as follows; 1. After one day of tooth movement, occlusion of blood vessels, hyalinization of periodontal ligament and resorption of alveolar bone adjacent to the alveolar crest on pressure side were observed. Above the tissue changes on the pressure side of group II were more severe than those of group I. Especially, septal bone of group II was separated after 2 days of tooth movement. 2. In tension zones, periodontal space was widened and periodontal fibers were orientated in the direction of puil. The blood vessels of periodontal ligament were distended. New bone deposition was seen along the inner surface of the alveolus after 2 days of tooth movement. 3. After 3 days of tooth movement, deposition of new bone was seen along the periosteal surface of alveolar bone on pressure side, progressing with increasing after removal of orthodontic force. Remodelling of the new bone was occurred 5 days after removal of orthodontic force. 4. 3 days after removal of orthodontic force, invasion of blood vessels into the marginal periodontal ligament on pressure side was observed clearly and the vessels below the epithelial attachment were increased. 5. After removal of orthodontic force, hyalinized structures disappeared concomittantly with an invasion of blood vessels from the neighboring periodontal ligament. 14 days after removal of orthodontic force, the vessels in the periodontal ligament of group I were finished the vascular rearrangement. 21 days after removal of orthodontic force, the vessels in the periodontal ligament of group II were finished the vascular rearrangement.
This study was performed to analyse the expression of VEGF and it's receptor(VEGFR) in the tension side of the periodontal ligament following orthodontic tooth movement. Upper first molars of Sprague-Dawley rats were moved medially using closed coil spring for 1, 2, 24 hours and 3, 7, 14 days. H&E staining, immunohistochemical staining and in situ hybridization methods were used to analyse the change of the expression of VEGF and VEGFR. The results from this study were as follows : 1. Following tensional force, periodontal ligament showed elongation of fibers, compression and congestion of vessels and regional hemorrhage. These tissue changes were recovered within 3 days of force application. New bone formation was seen after 3 days of force application and continued for the remaining experimental periods. 2. Following tensional force, VEGF and VEGF mRNA expression was increased in the periodontal ligament cells, osteoblasts and cementoblasts. This change was followed by increased vasculature in the periodontal ligament. 3. After 3 days of tensional force, VEGF and VEGF mRNA expression was confined mainly to the osteopaths and the periodontal ligament cells adjacent to the alveolar bone. After 2 weeks of force application, VEGF and VEGF mRNA expression was reduced to the level of control sample. 4. VEGFRs(Flt-1, Flk-1) showed similar expression pattern and it's expression was mainly seen in the endothelial cells and osteoblasts. Following tensional force VEGFR expression was increased in the endothelial cells and osteoblasts. In conclusion, in the tension side of the penodontal ligament, ligament cells, osteoblast and cementoblast showed increased expression of VEGF & VEGF mRNA. It preceded the increase of vasculature and new bone formation. The increased expression of VEGF mRNA in cementoblast may induce periodontal vessels, which distribute mainly the bone side half of periodontal ligament, grow in the direction of tensional force. Increased expression of VEGFR & VEGFR mRNA not only in endothelial cell but in osteoblast, osteocyte and periodontal cells showed VEGF acts not only in paracrine manner but in autocrine one.
The purpose of study is to investigate the difference of the daN force applied during the examination using Telos Device and the degree of fluctuation of anterior cruciate ligament according to the individual differences of the muscles involved in knee stability. The examination was done by the Lachman test using Telos Device and the changes of anterior cruciate ligament were measured by varying the force of 0. 15, 30 daN on each right and left side and the force of 30 daN after the bruce protocol. Computed tomography (CT) was used to measure muscle mass. As a result of measuring the degree of fluctuation of the anterior cruciate ligament according to the change of the force applied to the Telos Device, there was a statistically significant difference in the knee fluctuation when 15 daN and 30 daN were applied on both right and left. Also, it is analyzed that the degree of fluctuation of anterior cruciate ligament varies according to the difference of individual's muscle mass. Therefore, it is considered that the force applied to the Telos Device should be changed according to the difference of individual's muscle mass.
Journal of the Korean Society for Precision Engineering
/
v.25
no.4
/
pp.148-153
/
2008
Although manual traction, one of pain therapies, was applied in clinic to relief pain, the study was rare on the manual force and displacement of ligaments at knee joint during manual traction. The aim of this study is to quantify not only manual force at knee joint but also elongated displacement of joint ligament by C-arm scanning and motion analysis. Twenty-one healthy subjects were tested with manual traction from grade I to grade III under neutral position by a physical therapist. We calculated traction force using joint farces of both hands and elongated displacement of joint ligament were measured. The results showed that traction forces by C-arm scanning analysis were averagely 1.67-fold greater than those by motion analysis, but elongated displacements were instead averagely 2.36-fold smaller than motion analysis. Finally, we could estimate relationship between traction force and elongated displacement at knee joint by two methods.
The retraction of anterior teeth is one of the fundamental methods in orthodontic treatment and a proper position and angulation of anterior teeth after the retraction are very important for esthetics, stability, and function of teeth. In this research we analyzed, by Finite Element Method, the stress distribution on the periodontal ligament according to the variation of force and moment applied on the crown and predict the pattern of movement of maxillary central incisor. At the same time, the amount of force and moment caused by activation of the loop which was used for retraction of maxillary central incisor was analyzed by Finite Element Method. We observed the following results: 1) We could control the stress distribution on the periodontal ligament by proper moment/force ratio on maxillary right central incisor and predict the pattern of movement of maxillary right central incisor. 2) The amount of stress on the periodontal ligament as well as the moment/force ratio demanded by each pattern of movement increased as the destruction of alveolar bone was worse. 3) The moment/force ratio demanded by each pattern of movement decreased as the angle between the maxillary central incisor and occlusal plane decreased. 4) The force with the open loop was shown to be large compared to that with the closed loop. Also, the force with the helix decreased by 30% compared to that without the helix. 5) Under the same conditions we observed a larger moment/force ratio when the open loop and/or the helix were used.
The purpose of this study was to analyze the magnitude and mode of the stress distribution induced in the supporting alveolar bone and periodontal ligament and, to determine the displacement of abutment teeth and telescope denture base by applying chewing force to the telescope denture quantitatively and qualitatively. Two finite element models of telescope denture that were restored the missing mandibular second molar with two abutment teeth which were constructed. In two different models, parallel and tapering type telescope crowns were constructed. These finite element models of two cases used for these experiment were a two-dimensional mesiodistal section of the mandibular second bicuspid and first molar. Chewing force of 25Kg that was devided in the ratio of 45/155 (29%) in bicuspid and 55/155 (35.5%) in molars was applied to telescope denture and abutment teeth respectively. The displacement of the telescope denture base and abutment teeth and the stress distribution in the periodontal ligament and alveolar bone were analized to investigate the influence of chewing force acting on the telescope denture and abutment teeth. The results were as follows: 1. Abutment teeth displaced mesially and the magnitude of displacement of abutment teeth in vertical direction were more than that of horizontal direction in two cases. The displacement of abutment teeth on the telescope denture treated with tapering type telescope crown were less than that of the parallel type crown. 2. The displacement of the telescope denture base that were treated with parallel type telescope crown were less than that of treated with tapering type telescope crown. 3. The stress induced in the alveolar bone and periodontal ligament on abutment teeth that treated with parallel type telescope crown were more than that of treated with tapering type telescope crown and more stress induced in the alveolar bone than in the periodontal ligament. 4. In the telescope denture, the magnitude of displacement of abutment teeth and stress induced in the periodontal ligament and alveolar bone were within physiologic limit.
Journal of the Korean Society of Manufacturing Technology Engineers
/
v.18
no.3
/
pp.321-327
/
2009
This paper studied the influences of the main design parameter-the expansion angle and the material properties of the self-expansion anterior cruciate ligament fixation device on the contact condition with the bone and the initial stability of the device. Using finite element analysis, the stress distributions of the ring part of the device and the wall of the bone tunnel were calculated. And the micro-migration of the device by the pull-out force was calculated. From the analysis results, it was found that when designing the self-expansion type anterior cruciate ligament fixation device, it is desirable to use the material having higher Young's modulus and to design the fixation device that all wedges uniformly maintain contact with bone to obtain initial stability after operation.
We investigated the biomechanical properties of a newly designed self-expansion type anterior cruciate ligament (ACL) anchor. The ACL anchor consists of the ring section giving the elastic force, the wedge for maintaining in contact with the femur tunnel wall and the link suspending hamstring graft or artificial ligament. The main design parameters that determine the performance of this device were the expansion angle (${\theta}$) and the thickness ($t_R$). The Ti6Al4V anchors were heated after inserting in a jig for 1 hour at $800^{\circ}C$ in a protective argon gas atmosphere and allowed to cool to room temperature in the furnace. In order to investigate the influence of the expansion angle and the thickness of the ring on the biomechanical properties of the anchor, the maximum pull-out load, stiffness and slippage of the ACL anchor were measured using the pull-out tester, and statistical analyses were also executed. The present results showed that the design parameters gave a significant effect on the performance of the self- expansion type of anchor. The pull-out load of the ACL anchors significantly increased as the thickness of the ring section was increased, having a similar trend for both expansion angles. The ACL anchor showed about 2.5 times higher values of the pull-out load than that of the minimum load (500N)required for the "accelerated rehabilitation". The optimum ${\theta}$ and $t_R$ values of this ACL anchor were suggested to have sufficient resistance against the pull-out force, high stiffness and relatively low slippage after ACL reconstruction.
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