• Molecules and Cells
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• v.43 no.12
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• pp.1002-1010
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• 2020

Cilia are important eukaryotic cellular compartments required for diverse biological functions. Recent studies have revealed that protein targeting into the proper ciliary subcompartments is essential for ciliary function. In Drosophila chordotonal cilium, where mechano-electric transduction occurs, two transient receptor potential (TRP) superfamily ion channels, TRPV and TRPN, are restricted to the proximal and distal subcompartments, respectively. To understand the mechanisms underlying the sub-ciliary segregation of the two TRPs, we analyzed their localization under various conditions. In developing chordotonal cilia, TRPN was directly targeted to the ciliary tip from the beginning of its appearance and was retained in the distal subcompartment throughout development, whereas the ciliary localization of TRPV was considerably delayed. Lack of intraflagella transport-related proteins affected TRPV from the initial stage of its pre-ciliary trafficking, whereas it affected TRPN from the ciliary entry stage. The ectopic expression of the two TRP channels in both ciliated and non-ciliated cells revealed their intrinsic properties related to their localization. Taken together, our results suggest that sub-ciliary segregation of the two TRP channels relies on their distinct intrinsic properties, and begins at the initial stage of their pre-ciliary trafficking.

• Asia Pacific Journal of Business Review
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• v.6 no.2
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• pp.61-76
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• 2022

Based on the concept of advertising expenditure, manufacturing firm performance, and enterprise life cycle, this research conducts the listed Chinese manufacturing listed in 2016-2018 as the research sampling and divides these listed companies into three periods: growth, maturity, and decline. Next, this paper conducts empirical research from three aspects: advertising expenditure or investment and manufacturing firm performance, lag effect of advertising expenditure, and outcome effect and lag effect of advertising investment. It is found that in different stages of manufacturing enterprises, different advertising expenditures will have different impacts on the performance of manufacturing enterprises. In the growth stage, the advertising investment of manufacturing enterprises will significantly affect the performance results of the current period, and there will be the long-term lag effect. In contrast, the mature stage of enterprises' advertising investment has a shorter period of lag effect, while in the recession stage, the mature stage of manufacturing enterprises' advertising investment will have a shorter period of lag effect. The empirical results are not significant. This study provides a reference for manufacturing enterprises in different stages in the decision-making of advertising investment.

• Korean Journal of Radiology
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• v.21 no.6
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• pp.633-646
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• 2020

We reviewed all studies assessing the health-related quality of life (HRQoL) in patients with hepatocellular carcinoma (HCC) between 2009 and 2018 (n = 45). Most studies assessed HRQoL as an outcome, and evaluated or compared the HRQoL of HCC patients depending on the type of treatment or stage of disease. HCC patients had a worse HRQoL than the general population, including in those with early-stage HCC. Patients commonly experienced pain, fatigue, sleep disturbance, distress, and lack of appetite, and these symptoms remained problematic even a few years after treatment. TNM classification of malignant tumors stage, tumor stage, presence of cirrhosis, being Asian, being female, living alone, or being unemployed were associated with a poor HRQoL. While recent studies have included a more diverse patient population, various topics, and different study designs, there were limited studies on supportive interventions. Given the increase in HCC cases and HCC survivors, addressing the HRQoL of HCC patients requires more attention.

• Journal of the Korean housing association
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• v.6 no.1
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• pp.89-101
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• 1995

This study is to analyze dwelling environmental planning in old age of middle age. For this purpose. the data were collected by using questionnaire distributed to 332. The data were analyzed by Chisquare test. The major findings of this research were as follow: 1. In housing plan of old age. they prefer new house .that is not exisiting house. independent house and ordindry housing region in the suburbs of urban. 2. It is 69.3～99 square meter what size of house is suitable for independent life of aged person for living with children. it is 102.3～132 square meter. 3. In the stage of independent or semi-independent life. they want aged couple-independent life. In the stage of dependent life. they want to be cared with children. 4. The garden(madang) is the most important space for age couple in establishing housing plan. They want to amplity life recuperation center and job providing facilities. In old age housing project. they prefer housing type with facility that combine old age house with young age house. According to increasement of dependence. the service support system required flexi-care-system.

• Journal of the Society of Naval Architects of Korea
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• v.57 no.1
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• pp.1-7
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• 2020

In general, an inspection schedule is established based on the long-term fatigue life during the design stage. However, in the design stage, it is difficult to clearly identify the uncertainty factors affecting long-term fatigue life. In this study, the probabilistic fatigue life assessment was conducted in accordance with the methodology of DNV-GL. Firstly, The initial crack distribution estimated through the initial crack propagation analysis was updated by reflecting the results of crack inspection. Secondly, the updated crack distribution was compared with the initial crack distribution, and the probability of failure was updated with the effect of crack inspection.

• Explosives and Blasting
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• v.41 no.4
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• pp.9-16
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• 2023

To compare the global warming impact of the TBM and NATM method, which are representative tunnel excavation methods, a life cycle assessment was performed for each method. Life cycle assessment should compare the sum of carbon emissions by considering the pre-manufacturing stage, product manufacturing stage, usage stage, and disposal stage. However, access to TBM (Tunnel Boring Machine) manufacturing and disposal data is limited, so I had no choice but to focus on the analysis for the usage stage. In general, carbon emissions during the pre-product manufacturing stage and product manufacturing stage often exceed 90% of carbon emissions throughout the entire process. Therefore, since it is difficult to achieve the analysis goal only by comparing the usage stage, the analysis scope was expanded, and carbon emissions for the process were calculated for the NATM method with access to manufacturing data. As a result of comparing the relative impact on global warming, the carbon emissions of the TBM method were found to be higher than those of the NATM method even though TBM method was only considered for the usage stage. So there it is, the NATM method can be seen as environmentally friendly in the future when considering the impact of climate change (global warming), which has recently attracted attention among environmental impact fields.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.266-277
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• 2014

Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. In the SCNT experiment, nuclear reprogramming is induced by exposure of introduced donor nuclei to the recipient cytoplasm of matured oocytes. However, because the efficiency of SCNT still remains low, a combination of SCNT technique with the ex-ovo method may improve the normal development of SCNT embryos. Here we hypothesized that treatment of somatic cells with extracts prepared from the germinal vesicle (GV) stage Siberian sturgeon oocytes prior to their use as nuclear donor for SCNT would improve in vitro development. A reversible permeability protocol with $4{\mu}g/mL$ of digitonin for 2 min at $4^{\circ}C$ in order to deliver Siberian sturgeon oocyte extract (SOE) to porcine fetal fibroblasts (PFFs) was carried out. As results, the intensity of H3K9ac staining in PFFs following treatment of SOE for 7 h at $18^{\circ}C$ was significantly increased but the intensity of H3K9me3 staining in PFFs was significantly decreased as compared with the control (p<0.05). Additionally, the level of histone acetylation in SCNT embryos at the zygote stage was significantly increased when reconstructed using SOE-treated cells (p<0.05), similar to that of IVF embryos at the zygote stage. The number of apoptotic cells was significantly decreased and pluripotency markers (Nanog, Oct4 and Sox2) were highly expressed in the blastocyst stage of SCNT embryos reconstructed using SOE-treated cells as nuclear donor (p<0.05). And there was observed a better development to the blastocyst stage in the SOE-treated group (p<0.05). Our results suggested that pre-treatment of cells with SOE could improve epigenetic reprogramming and the quality of porcine SCNT embryos.

• Proceedings of the KSR Conference
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• 2007.05a
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• pp.584-588
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• 2007

A comparative Life Cycle Assessment(LCA) among three types of Electric Motor Unit(EMU) Interior Panel(IP) was conducted. A functional unit for comparative LCA is a weight of IP for 1 EMU. It assumed that Manufacturing stage and its upstream processes, Use stage and End of Life(EoL) stage are included in the boundary of product system. For Use stage, the weight of IP causes electricity consumption. It is assumed that aluminum IP is recycled and the other IPs are incinerated at the EoL stage. As comparison results, aluminum IP has much larger environmental impact(5.162pt) than others(FRP IP; 4.069pt, Phenol IP; 4.053pt) even though recycling consideration is included. The manufacturing stage of aluminum has relative big envrionmental impact(1.824pt) and this point make the most important difference from other IPs(FRP IP; 0.1617pt, Phenol IP; 0.4534pt)). Despite of large weight difference between FRP IP(888.56Kg) and phenol IP(315Kg), the final environmental impact result has only little difference(0.016pt, 0.39%). With this result, the EMU designer can choose IP with a consideration of the environmental performance of IP.

• Korean Journal of Community Nutrition
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• v.20 no.2
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• pp.129-140
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• 2015

Objectives: We examined the association between the adherence to dietary guidelines for breast cancer survivors and health-related quality of life in a cross-sectional study of Korean breast cancer survivors. Methods: A total of 157 women aged 21 to 79 years who had been diagnosed with stage I to III breast cancers according to the American Joint Committee on Cancer (AJCC) and had breast cancer surgery at least 6 months before the baseline were included. We used a Korean version of the Core 30 (C30) and Breast cancer 23 (BR23) module of the European Organization for Research and Treatment Cancer Quality of Life Questionnaire (EORTC-QLQ), both of which have been validated for Koreans. Participants were asked about their adherence to dietary guidelines for breast cancer survivors, suggested by the Korean breast cancer society, using a 5-point Likert scale. We summed dietary guideline adherence scores for each participant and calculated the least squares means of health-related quality of life according to dietary guideline adherence scores using the generalized linear model. Results: Breast cancer survivors who had higher adherence to dietary guidelines for breast cancer survivors had lower constipation scores than those with lower adherence (p for trend=0.01). When we stratified by the stage at diagnosis, this association was limited to those who had been diagnosed with stage II or III breast cancers. Also, sexual functioning scores increased significantly with increasing adherence scores of dietary guidelines among those with stage II or III breast cancers (p for trend < 0.001). However, among those who had been diagnosed with stage I, higher scores of dietary guidelines were associated with higher scores of pain (p for trend=0.03) and breast symptoms (p for trend=0.05). Conclusions: Our study suggested that the health-related quality of life levels of breast cancer survivors are associated with the adherence to dietary guidelines and may differ by the stage of the breast cancer.

• Journal of Ginseng Research
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• v.41 no.1
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• pp.23-30
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• 2017

Background: Ginsenoside Rg1 is a class of steroid glycoside and triterpene saponin in Panax ginseng. Many studies suggest that Rg1 suppresses adipocyte differentiation in 3T3-L1. However, the detail molecular mechanism of Rg1 on adipogenesis in 3T3-L1 is still not fully understood. Methods: 3T3-L1 preadipocyte was used to evaluate the effect of Rg1 on adipocyte development in the differentiation in a stage-dependent manner in vitro. Oil Red O staining and Nile red staining were conducted to measure intracellular lipid accumulation and superoxide production, respectively. We analyzed the protein expression using Western blot in vitro. The zebrafish model was used to investigate whether Rg1 suppresses the early stage of fat accumulation in vivo. Results: Rg1 decreased lipid accumulation in early-stage differentiation of 3T3-L1 compared with intermediate and later stages of adipocyte differentiation. Rg1 dramatically increased CAAT/enhancer binding protein (C/EBP) homologous protein-10 (CHOP10) and subsequently reduced the $C/EBP{\beta}$ transcriptional activity that prohibited the initiation of adipogenic marker expression as well as triglyceride synthase. Rg1 decreased the expression of extracellular signal-regulated kinase 1/2 and glycogen synthase kinase $3{\beta}$, which are also essential for stimulating the expression of $CEBP{\beta}$. Rg1 also reduced reactive oxygen species production because of the downregulated protein level of nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase 4 (NOX4). While Rg1 increased the endogenous antioxidant enzymes, it also dramatically decreased the accumulation of lipid and triglyceride in high fat diet-induced obese zebrafish. Conclusion: We demonstrated that Rg1 suppresses early-stage differentiation via the activation of CHOP10 and attenuates fat accumulation in vivo. These results indicate that Rg1 might have the potential to reduce body fat accumulation in the early stage of obesity.