• 원예과학기술지
• /
• 제28권5호
• /
• pp.790-795
• /
• 2010

본 연구는 절화 아이리스 '블루매직'의 수명에 미치는 cycloheximide(CHI)와 보존용액의 효과를 알기 위해 실시하였다. 아이리스의 절화수명은 증류수구에 비해 3% sucrose (S) + $100mg{\cdot}L^{-1}$ HQS + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ BA 또는 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ CHI, 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI 처리구에서 현저히 길었다. $200mg{\cdot}L^{-1}$ HQS를 보존용액에 첨가했을 때 나타나는 줄기 기부의 쪼그라듬 현상은 $100mg{\cdot}L^{-1}$ HQS 처리 시 완전히 없어졌다. 3% S + $100mg{\cdot}L^{-1}$ HQS + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ BA 처리는 절화수명, 생체중, 수분균형, 개화에 가장 효과적이었다. 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ CHI, 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI 보존용액 처리시 용액흡수량과 증산량은 낮았으나 수분균형은 높았다. 반개화 상태에서 수확한 아이리스에 CHI 처리는 노화를 지연시켰고 절화수명도 현저히 증가시켰다. 봉오리 단계의 아이리스에 CHI를 함유한 보존용액을 처리하면 개화가 잘 이루어지지 않으나, 꽃잎이 벌어진 상태에서 CHI를 함유한 보존용액 처리를 하면 개화가 완전하게 이루어졌다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제17권2호
• /
• pp.175-180
• /
• 2013

Resveratrol is a phytoalexin found in grapes, red wine, and berries. Resveratrol has been known to have many beneficial health effects, such as anti-cancer, neuroprotective, anti-inflammatory, and life-prolonging effects. However, relatively little is known about the effects of resveratrol on the regulation of ligand-gated ion channels. We have previously reported that resveratrol regulates subsets of homomeric ligand-gated ion channels such as those of 5-$HT_{3A}$ receptors. The ${\gamma}$-aminobutyric $acid_C$($GABA_C$) receptor is mainly expressed in retinal bipolar cells and plays an important role in visual processing. In the present study, we examined the effects of resveratrol on the channel activity of homomeric $GABA_C$ receptor expressed in Xenopus oocytes injected with cRNA encoding human $GABA_C$ ${\rho}$ subunits. Our data show that the application of GABA elicits an inward peak current ($I_{GABA}$) in oocytes that express the $GABA_C$ receptor. Resveratrol treatment had no effect on oocytes injected with $H_2O$ or with $GABA_C$ receptor cRNA. Co-treatment with resveratrol and GABA inhibited $I_{GABA}$ in oocytes with $GABA_C$ receptors. The inhibition of $I_{GABA}$ by resveratrol was in a reversible and concentration-dependent manner. The $IC_{50}$ of resveratrol was $28.9{\pm}2.8{\mu}M$ in oocytes expressing $GABA_C$ receptor. The inhibition of $I_{GABA}$ by resveratrol was in voltage-independent and non-competitive manner. These results indicate that resveratrol might regulate $GABA_C$ receptor expression and that this regulation might be one of the pharmacological actions of resveratrol on the nervous system.

• 대한예방한의학회지
• /
• 제8권2호
• /
• pp.141-156
• /
• 2004

Objectives : JianTeng-Yuan(交藤圓) is said to be a prescription for preservation of health in ${\ulcorner}$HuaTuo ZhongZangJing(華陀 中藏經)${\lrcorner}$. It is known to have the effect of Bu-Shen(補腎: strengthening kidney) and Yi-Shou(益壽: prolonging the span of one's life). This study investigates whether JTY is effective on inhibition of oxidation stress. Methods : Sprague-Dawley Rats(12-week-old, weight $300{\pm}20g$) were divided into 3 groups. Normal group(n=8) was injected PBS(1ml/body, s.c) at the back neck's skin. Control group(n=8) was injected D-galactose(50mg/kg, 1ml PBS/body, s.c) to induce pathological animals. JTY group was injected the same treatment for the Control group, and fed containing JTY(10%). The whole groups were treated 1 time per day for 6 weeks. After rats were sacrificed and anti-oxidant enzyme(SOD, CAT, G-px) activity, GSH quantity of RBC and tissue(heart, liver and kidney), plasma Vit-C quantity were examined. Besides, the MDA levels of liver and kidney, lipofuscin of heart and endurance of erythrocyte membrane were measured. Results : In the JTY group, RBC's SOD activity decline was halted by 21% of the normal level, compared to the control group ; G-px activity(unit/g of Hb) increased significantly, compared to the normal group ; and the level of Vit-C in plasma increased by 16%. Heart's SOD activity was kept at the same level as that of the normal group ; and CAT activity decline was halted by 26%. Kidney's CAT and G-px activities were kept at the same level as that shown in the normal group, implying the existence of halting effect. Liver also showed a slight halting effect against the decline of anti-oxidant ability, but the effect was not significant(a=0.05). A comparison between the levels of peroxide in SD rats showed that the level of TBARS in plasma increased significantly in the control group and that it was normal in the JTY group. The livers in the JTY group, compared to those in the control group, showed 36% halting effect of the normal level while their kidney's indicated the level significantly lower than the normal level. Heart's lipofuscin increased significantly in the control group, but was alike in both the JTY and the normal groups. Endurance of erythrocyte membrane(%) decreased significantly in the control group while it was kept at the similar level in both the JTY and the normal groups, indicating the halting effect. Conclusions : This study suggests that JTY is effective to defend oxidation stress caused by D-galactose in the animals. It showed that the anti-oxidant ability was maintained and strengthened. On the other hand, it reduced the level of peroxide in animals. In sum, JTY appeared to have the equilibrium normal physiological function in SD rat.

• 한국포장학회지
• /
• 제21권1호
• /
• pp.35-40
• /
• 2015

결구상추는 급식업체에서 로컬리즘을 바탕으로 한 요구로 인해, 국내 생산품의 주년 공급이 필요하다. 그러나 하절기에 고온과 강우로 인해 적절한 결구상추의 공급이 원활하지 않다. 따라서 결구상추를 지속적으로 미리 저장하여, 출하가 어려울 때도 원활하게 공급하고자 하였다. 본 연구에서는 포장 및 저장방법에 따라 결구상추를 관행적으로 수확하여 PP플라스틱 농산물 유통상자에 단순 저장과, 결구상추의 줄기(경정) 10 cm 이상 남겨 부직포에 감싸서 저장, 상자에 농산물 식품 포장재인 LDPE 미세천공 필름을 덮어 저장하였으며, 또한 개별적으로 LLD-PE 포장재로 랩 포장하여 저장하였으며, 저장 온도는 $2^{\circ}C$에서 수행하였다. 저장 후의 포장 방법별 외관품위 및 생체중 감소에서는 개별적으로 랩 포장한 것이 21일까지의 단기적 저장에서 가장 좋았으며, 줄기를 보존하여 저장한 것, 상자에 필름 외피복, 무포장 저장 순이었다. 따라서 결구상추는 단기저장 시에 개별적인 포장 저장(저장방법 C)이 저장 3주까지 비교적 선도가 좋아, 결구상추의 단경기 원활한 공급을 위해 보급 가능한 기술로 보인다. 줄기를 물올림하면서 보존하는 방법(저장방법 D)은 재배방법과 연계하여 방법을 개선한다면, 새로운 저장방법으로 강구(講究)될 수 있을 것으로 예상된다. 결구상추의 단경기 공급을 위해서 생산이 어려운 시기에 포장과 저장방법을 개선함으로써 수급조절에 원활히 할 수 있는 가능성을 확인하였으나, 보다 선도를 유지하기 위해서는 국내 생산될 저장과 유통여건에 맞춘 추후의 연구가 더 필요할 것으로 생각된다.