• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.207-213
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• 2014

The structural proteins of classical swine fever virus (CSFV) consist of nucleocapsid protein C and envelope glycoprotein $E^{rns}$ (E0), E1 and E2. Among them, E2, the most immunogenic of the CSFV glycoproteins, induces a protective immune response in swine. In this study, to determine the optimal expression conditions of glycoprotein E2 using baculovirus system, we investigated the influence of insect cells and media to the expression of recombinant E2. Recombinant virus containing glycoprotein E2 coding gene was constructed with bApGOZA DNA. Expression of the glycoprotein E2 was analyzed by SDS-PAGE and Western blot analysis using anti-CSFV E2 monoclonal antibodies. Expression of glycoprotein E2 in Sf21 cells was first observed after 3 days and reached a maximum on the 5th day after infection. Furthermore, the highest levels of glycoprotein E2 expression were observed at multiplicity of infection (MOI) of 5. When three different insect cell lines (Sf21, High-Five and Se301) were tested, High-Five cells showed the highest production. In addition, four different serum-free and serum-supplemented media, respectively, were tested for the expression of glycoprotein E2 and the budded virus (BV) titers. As a result, serum-supplemented medium provided the best conditions for protein production and the BV yield.

• 펄프종이기술
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• 제40권1호
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• pp.29-34
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• 2008

The charcoal has been used not only as fuel but also as human health care material since it was used. Charcoal's performances were generally investigated in aspects of energy efficiency and caloric values. This study was conducted in order to increase charcoal's application area and to develop functional paper. Five types of charcoal were used on a basis of gas absorption properties from previous study. Handsheets were made by two methods by internal loading and surface spray on interlayer. Strength properties of internal loading and interlayer spray handsheets were decreased as the charcoal loading increased. Ethylene gas absorption property was higher in both of oak's black charcoal and white charcoal than others. In terms of strength, 5-10% charcoal loading was efficient. Above 10% loading, a rate of strength decrease was higher than that of ethylene gas absorption rate. Formaldehyde absorption property was higher at both of oak's black charcoal and mixed charcoal than others. However, to guarantee enough charcoal loading should be higher than 95 $g/m^2$ for sufficient formaldehyde absorption.

• 대한가정학회지
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• 제38권11호
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• pp.89-104
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• 2000

The purpose of this study is to grope the direction of the environmental movement for the improvement of welfare. The deterioration of environment which was resulted from the development of material civilization due to the industrialization made people newly recognize the importance of environment. The quality of the future life depends on the solution of the environmental problems. The family-centered environmental movement as a new cultural movement in the daily life should be revitalized for the purpose of improving welfare. This study investigated the several views on the environmental problems in the neighboring disciplines of home economics. And on the basis of the results of the study, the direction of environmental movement to be developed in home economics was represented. This study represented the directions of the environmental movement as a new cultural movement in the daily life as follows: alternative life-style, that is, pro-environmental life-style-oriented direction; community-oriented direction such as cooperative association movement in daily life; the politicalization of the environmental problems, that is, the endeavor to reflect the election promise on the environment in the vote, etc. The content of the environmental movement shoed be treated of in environmental education, consumer education, family-life education, school education, and life-long education.

• 산경연구논집
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• 제8권5호
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• pp.35-42
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• 2017

Purpose - This study investigated small business members' cognition of happiness in accordance with changes of employment environment at the nation's industrialization. The study verified effects of labor workers' psychological wellbeing conditions upon life satisfaction as well as the effects of job burden and family burden of employment instability. Research design, data, and methodology - The subjects were small business workers in Seoul and Metropolitan areas. The author collected 150 copies of effective questionnaires. Regression analysis, hierarchical analysis and 3-stage mediation regression analysis were done. Results - The findings of this research was that psychological wellbeing factors alleviated job burden and family burden to increase satisfaction with life. Employment instability moderated between job burden and satisfaction with life, job burden between environmental control and satisfaction with life, and family burden between goal and satisfaction of life. Members needed sub factors of psychological wellbeing, especially reflection for self-acceptance. Conclusions - This study inspected effects of small business workers' psychological wellbeing upon life satisfaction at changes of management environment as well as labor environment to verify effects of job burden, family burden and employment instability and to find out conditions of human resources management from point of view of small business and workers.

• 분석과학
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• 제27권3호
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• pp.153-160
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• 2014

세계적으로 석면분석방법에 관한 다양한 연구사례가 발표되고 있는 가운데, 분석방법의 차이에 따른 석면함유물질(ACM, Asbestos Containing Material) 결과에 대한 관심이 대두되고 있다. 본 연구에서는 균질성이 확보된 ACM 시료를 시야평가법(visual estimation method)과 포인트계수법(point counting method)의 분석결과에 대한 비교 평가를 통하여 분석기관 및 분석자의 신뢰성 향상과 더불어 편광현미경 분석의 기초자료를 확보고자 하였다. 석면분석은 정성분석과 정량분석으로 구분하였으며, 석면정량을 위한 방법은 EPA 600-R-93-116의 시야평가법과 포인트계수법으로 총 400지점을 계수하는 방법으로 실시하였다. 먼저 준비된 시료의 균질성을 분산분석을 이용하여 확인한 결과 이상이 없음을 확인하였다. 정성분석의 결과는 백석면과 갈석면으로 확인되었고 정량분석결과는 포인트계수법이 시야평가법과 비교하여 전체적인 석면함유량이 저평가되는 경향과 함께 포인트계수법은 분석과정이 조금 복잡하고 분석시간이 많이 소요되었다.

• 한국응용곤충학회지
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• 제57권1호
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• pp.43-50
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• 2018

4종의 diamide계열 살충제를 이용한 배추좀나방과 파밤나방의 지역계통별 감수성을 조사하였다. 배추좀나방의 경우 성주와 거창지역 집단에서 4종의 약제 모두 추천농도에서 100%의 살충활성을 보인 반면, 평창지역 집단에서는 chlorantraniliprole에 대해 42.3%의 낮은 살충활성을 보였다. 감수성계통 배추좀나방과 저항성비를 비교한 결과 cyclaniliprole은 비교적 4지역에서 유사하거나 낮은 저항성비(0.1~6.3배)를 보인 반면, 평창지역 집단은 chlorantraniliprole (1,196.3배)와 cyantraniliprole (105.6배), flubendiamide (191.6배)은 매우 높은 저항성비를 보였다. 파밤나방의 경우 청주와 진도, 영광지역의 집단 모두 4종의 약제에 낮은 감수성을 나타났는데, 특히 청주와 진도지역의 집단은 flubendiamide에 대해 100,000배 이상의 저항성비를 보였다. 채집 연도(2014년과 2017년)에 따른 파밤나방에 대한 chlorantraniliprole의 감수성을 비교한 결과 2014년에 채집된 집단들은 모두 100%의 살충률을 보인 반면 2017년에 채집된 집단들은 살충활성이 매우 낮게 나타났다. 본 연구결과는 diamide계열 살충제가 빠르게 저항성이 발현되고 있으며 작용기작이 다른 약제와의 교호살포 등 종합적 방제전략을 수립하는데 기초자료가 될 수 있을 것이다.

• 한국식생활문화학회지
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• 제19권1호
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• pp.61-69
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• 2004

The shelf-life of Yugwa(Busuge) is very short because of the low oxidative stability of soybean oil which is conventionally used as the frying oil of Yugwa base with high porous structures. To identify the possibility of replacing soybean oil with palm oil whose oxidative stability is high, POV(peroxide value), AV(acid value), colorness (L. a and b), hardness and sensory characteristics during storage of the Yugwa base fried with the soybean oil and palm oil stored in the anaerobic packaging or the aerobic packaging at 10 and $30^{\circ}C$ for 75 days were measured. POV and AV of the Yugwa base fried with palm oil were significantly lower than those of Yugwa fried with the soybean oil. Hardness and colorness were not different each other and, sensory evaluation was slightly higher than that fried with soybean oil. Lipid absorption amount was not significantly different between the Yugwa base fried with palm oil and the one fried with soybean oil.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제18권1호
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• pp.45-52
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• 2012

This study aims to examine the outdoor environment of a geriatric hospital and explore its stress-relief impact upon those who utilize it. Post Occupancy Evaluation is employed to assess the effectiveness of a healthcare setting. This study is based on survey questionnaire including the users' perception of outdoor environment, their perceived restorativeness, and quality of life. A total of one hundred and sixty individuals including patients, families and visitors, and healthcare staffs. As a result, it turns out that the therapeutic outdoor environment and its users' health outcomes, such as reststroativeness and quality of life are significantly correlated. In conclusion, the finding of this research seems to demonstrate the potential that the therapeutic outdoor environment has for the improvement of its users' health by enhancing.

• 한국주거학회논문집
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• 제25권1호
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• pp.73-80
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• 2014

Korea is quickly transforming into an aging society. As a reaction to the accelerated aging society in recent years, there is a growing need to introduce sustainable housing policies and strategies for aging society. The main purpose of this study is to explore where people want to live after retirement. Especially, this study focuses on the residential environment such as housing types, residential location, and living environment. The main findings are as follows. First, people preferred living in a sububan-style housing in the suburban or rural areas to living in a city-style housing in the urban areas. Second, people have a strong preference for the importance of convenience in using natural environment, medical service, and cultural facilities. Third, the family life style has been changed from children-oriented life style to couple-oriented life style by showing that people like to stay with a spouse rather than with their children after retirement.

• 대한안전경영과학회지
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• 제8권1호
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• pp.113-130
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• 2006

As environmental damage increase by a highly developed material civilization of today, many companies take a growing immensely interest in the influence of environment for beginning a new paradigm year by year. The previous assessments dose not run the gamut of industry but is confined within a certain facility or an area. Industrial processes and operations can not be accomplished independently but are connected with each others through suppliers and customer, and these ideas are fundamental notions of Life Cycle Assessment(LCA). This paper will introduce Life Cycle Assessment(LCA) in environment which is rising, and would like to build environmental management system using approach of Quality Function Deployment(QFD) and Safety Function Deployment(SFD) belonging to the assessment method.