• Journal of fish pathology
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• v.34 no.2
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• pp.177-184
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• 2021

Vaccines based on single-cycle viruses that are replication-incompetent due to knockout of replication-related structural gene(s) are more immunogenic than inactivated or subunit vaccines and can be used as delivery vehicles for foreign antigens without concerns on the reverting to virulent forms. The aim of this study was to develop a delivery vehicle for nervous necrosis virus (NNV)-like particles (VLPs) using G gene deleted single-cycle VHSV (rVHSV-𝚫G). Recombinant single-cycle VHSVs carrying NNV capsid protein gene between N and P gene of rVHSV-𝚫G genome (rVHSV-𝚫G-NNV_Cap) were rescued by reverse genetic technology. The successful expression of NNV capsid protein in cells infected with rVHSV-𝚫G-NNV_Cap was demonstrated by Western blot analysis, and the production of NNV VLPs in infected cells was confirmed using an electron microscopy. The results suggest that single-cycle VHSVs can be used as a safe delivery vehicle for NNV VLPs, and can be extended to other pathogens for the development of prophylactic vaccines.

• Journal of Families and Better Life
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• v.2 no.1
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• pp.35-55
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• 1984

This study aims to present basic data for a reasonable home management through investigating the change of home economy conditions incidental to the family life cycle, also through analyzing the effect of socio-anthropological variables on the family-life cycle. The data investigation using the questionnaires method was conducted on housewives in Seoul as the central census tract. Housewives as the subject of investigation were chosen by the method of the purpose-sampling in consideration of the regional differences and the socio-economical strata. Nine hundred and forty questionnaires were distributed to housewives but seven hundred questionnaires were collected. Only five hundred and ten questionnaires of them were analyzed in this study. The frequency and the percentage of collected data, first of all, were founded in order to grasp the general characteristics of the subject of investigation. To classify the stage of family life cycle, the correlations of the classifying factors among each group were examined x2 Test and One-Way ANOVA were applied to explore the differences among each stage of the change of household-economy. And the effect of socio-anthropological variables on the change of household- economy was analyzed by Two-way ANOVA. the conclusions derived from the results of this study are as follows; 1) The marriage-period and the educational conditions of the first child were selected as the classifying factors through analyzing correlation among the age, the marriage-period and the educational conditions of their children. As a result of this analysis, the family life cycle were classified into seven stages: Stage 1; Establishment Stage 2; Preschool family Stage 3; Elementary school family stage 6; Adult period family Stage7; Marriage period family . 2) The change of household economy incidental to the progress of family life cycle has a significant differences in all of variables (except the other member of family's income) Stage 1; Though the husband's income and the income from property are on a low level, the total income is on a high level due to the housewife's income. Stage 2; The total income is on a low level owing to the decrease of house wife's income, though the husband's income keep growing. Stage 3; Owing to the increase of husband's income, the cost of living as well as the total income keeps growing but the savings are on the decrease. Stage 4; Compared with Stage 3, the total income tends to be on a low level but the living expenses are on the increase. Stage 5; The husband's income is on the highest level in the stages of family life cycle. The total income and the living expenses are on a high level. Stage 6; The income of husband and housewife are on the remarkable decrease but the children's income is on the increase. Stage 7; Owing to the increase of the children's income and the income from property, the total income is on the highest level in the stages of family life cycle. 3) Considering the effect of socio-anthropological variables on the conditions of household-economy, family system has an significant effect on children's income. the husband's occupation exerts a significant effect upon the housewife's and children's income. The husband's schooling exercises an effect upon the children's income. S.E.S has a important effect on the income of husband, housewife and children. From the above results, it is found that the change of household-economy conditions is incidental to the progress of family life cycle. Therefore, a suitable measure to cope with the desire of family and the conditions of household-economy should be prepared, in order to carry on a reasonable home management.

• Journal of the Korean Society of Safety
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• v.33 no.3
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• pp.8-14
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• 2018

Ni-base superalloy has excellent resistance to extreme environments such as high temperatures and high stresses and are used as materials for large gas turbines. In this paper, the specimens were taken from the blade that were used for a long time, and their life span was studied by microstructure analysis and avoidance of cursing. The microstructural analysis of the specimens was carried out using a OM and SEM to observe the coarsening, carbides on gamma prime. Low-cycle fatigue tests were performed on new material and airfoil of long time-used blade. The test was conducted under various deformation conditions and temperature conditions of $760^{\circ}C$ and $870^{\circ}C$. The low cycle fatigue test was carried out using the Coffin-Manson equation and the fatigue life was predicted. After the test, crack path and fracture surface were analyzed using SEM.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2006.05a
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• pp.20-24
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• 2006

In this paper we investigated the method of examining the accelerated life test on LED traffic signal module by the temperature. The longevity presumption of the LED type signal light by a general heat cycle test used and executed cycle when it was done to longevity by the heat cycle test number and the acceleration factor of a real system requirements of this heat cycle test. Therefore, it reports on the introduction of the acceleration type from which the LED traffic signal module is done here to clarify the above-mentioned acceleration factor with the object and the acceleration factor is requested the test atmosphere actually in the system requirements.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.05a
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• pp.18-21
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• 2009

The life of components under high thermal load is typically shorter than other components. The turbopump turbine of liquid rocket is operated under these environments like high temperature and high centrifugal dorce due to high rotating velocity during operating time. These conditions may often cause low-cycle fatigue problem in the turbopump turbine. First of all, to analyze heat stress, ABAQUS/CAE is used and Coffin-manson's equation is used to consider elasticity and plasticity strain. S.W.T's method is used to consider the mean stress effect, using strain history, low-cycle fatigue analysis is done for turbopump turbine which may have FCL(fracture critical location). In this paper, strain life method is applied to analyze low-cycle fatigue.

• Architectural research
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• v.7 no.1
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• pp.11-18
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• 2005

In today's building industry the emphasis has been geared more towards construction, thus building maintenance and life cycle have been neglected until now. A direct result of this neglect is the rapid aging of building, which leads to more cost-effective decision making methods for the prolongation of building life span. The following study is conducted in the area of Daegu and Seoul in order to develop the estimation analysis method of the annual operation cost of the Korean high-rise condominiums for the cost-effective decision making support through mathematical and statistical analyses including the present value and standardized measurement corrections. Based on the assumption that the life expectancy of the high rise condominium is 50 years, initial cost is ￦421,212/$m^2$, and a total sum of yearly operation cost during life expectancy is ￦2,154,499//$m^2$), yearly accumulated operation cost is shown as below: $AOC=0.7097t^4-38.803t^3+806.95t^2+11045t-496.52$ ($R^2=0.98$) (Here, AOC = Accumulated Operation Cost, t = given years)

• Journal of the Korea Institute of Building Construction
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• v.8 no.1
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• pp.97-102
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• 2008

The report of the Korean Board of Audit and Inspection(BAI) on May 2007 indicates the problems of Life Cycle Cost (LCC) analysis and evaluation in the Design-Build(Turn-Key) and alternative bidding system. The point which the report indicates is that the cost estimation system for LCC analysis has nothing in common each other and there's no consistency among the repair cycle and ratio per facilities parts. For solving these problems, BAI demands the establishment of the guidelines for LCC analysis and evaluation from the competent authority Korean Ministry of Construction And Transportation(MOCT). The objective of this study is to develop the improvement directions for LCC analysis and evaluation which are suitable to the public construction projects especially for the Design-Build and alternative bidding system in Korea. For this study, the LCC related raws and regulations, LCC analysis guidelines of public cooperations, actual condition of LCC analysis and evaluation which include, the elements of LCC, the estimation rules of the initial cost and the maintenance cost, the analysis standards of time value of money, etc. are investigated to provide the improvement directions for LCC analysis and evaluation.

• Journal of the Korean Wood Science and Technology
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• v.20 no.3
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• pp.29-37
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• 1992

This research was carried out to investigate the effects of cyclic humidification on the dimensional stability of particleboard manufactured according the three specific gravity levels and com-ply fabricated with a core of particleboard and veneer or plywood as the face and back materials. Both the particleboard and com-ply were subjected to four cycles of 50-90-50% relative humidity. The results are summarized as follows: Particleboard and com-ply followed a clear pattern of increasing dimensions and weight on the adsorption cycle and decreasing dimensions and weight on the humidification cycle. After the fourth cycle, the dimensions and weight of both particleboard and com-ply were greater than those originally measured. The greater part of increase in occurred during the first humidification cycle. The specific gravity of particleboard has a significant effect thickness and volume change. The dimensional stability of com-ply was better than that of particleboard. In addition, the dimensional stability of com-ply bonded with plywood was better than that of com-ply bonded with veneer.

• Journal of Korean Academy of Nursing
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• v.47 no.4
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• pp.562-574
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• 2017

Purpose: This study attempts to explore the subjective experience of frailty among elderly individuals in Korea. Methods: From June to August in 2014, 11 elderly persons who had experienced frailty in a community were interviewed. For data analysis, the method suggested by Colaizzi was applied as a phenomenological method. Results: According to the analysis, the study participants' frailty process was structured in seven categories: (a) 'natural phenomenon with ageing,' (b) 'life force comes to an end,' (c) 'the light in my heart turns off,' (d) 'unavoidable situation,' (e) 'continuous and connected vicious cycle,' (f) 'the limit of recovery energy already passes,' and (g) 'life is supported by someone's help.' Conclusion: The frailty experience in the participants is a natural process of aging, which cause vicious cycle acting with each other among physical, psychological, and social health. It is said that the cycle of frailty was started from weight loss and insufficient sleep, and boostered by pain. The participants from repetition of the vicious cycle become exhausted and pass the threshold of their recovery energy at some points. If they meet with sudden accidents such as falling, traffic accident and so on, they become to live a dependent life supported by someone's help in a moment. To prevent frailty and worsening conditions in Korean elderly individuals, it is recommended to provide a interventional programs using this study's results.

• Journal of Electrical Engineering and Technology
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• v.13 no.4
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• pp.1631-1637
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• 2018

Electric utilities has been considered the necessity to introduce asset management of electric power facilities in order to reduce maintenance cost of existing facilities and to maximize profit. This paper aims to provide data that can helpful to make profitable decision in terms of power transformers which have a significant part in the power system. Therefore, this study is modeling input cost for power transformer during its entire life and also the life cycle cost (LCC) technique is applied. In particular, the variation of transformer state related with maintenance and the variation of the EUAC curve based on cost and effect of maintenance is examined. In this study, the trend of the equivalent uniform annual cost (EUAC) according to maintenance cycle and cost of equipment is analyzed. In line with that, sensitivity analysis influenced by the changes of other cost factors was performed.