• International Journal of Industrial Entomology and Biomaterials
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• v.15 no.2
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• pp.115-122
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• 2007

As the genome of B.mori is available in GenBank and the EST database of B.mori is expanding, identification of novel genes of B.mori is conceivable by data-mining techniques. We used the in silico cloning method to get the vacuolar-type $H^+-ATP$ synthetase (V-ATPase) c subunit (16 kDa proteolipid subunit) gene of B.mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR and sequencing. The V-ATPase c subunit cDNA contains a 468 bp ORF. The ORF encoded a 155-residue protein that showed extensive homology with V-ATPase c subunits from other 15 species and contained four membrane-spanning helices. Tissue expression pattern analysis revealed that V-ATPase c expressed strongly in Malpighian tubules, not in fat body. This gene has been registered in GenBank under the accession number EU082222.

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.1633-1640
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• 2007

The Five Days Work System started to be lately introduced among Korean companies is causing much interests in diverse aspects. This theme of '5 days work' seems like a hot issue both for academian and for practitioner in the respect that it incurred a great change and impact in worker's life. The purpose of this article is to depict the pattern and the effect incurred by '5 days work system', focusing on the female workers in railroad-related organizations. To attain this purpose, several aspects of current '5 days work system' and its effects both on work life and on family life are surveyed through questionaire and analyzed by SPSS. And some implications will be supposed. in based on the cognitive response of employees.

• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.68-73
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• 2004

Ferritin is an iron storage protein found in most living organisms. For expression and industrial use, human light chain ferritin (L-ferritin) was cloned from human liver cDNA library and expressed in Pichia pastoris strain GS115. The recombinant L-ferritin in Pichia pastoris was glycosylated. In a fed-batch culture, the cell mass reached about 57 g/l of dry cell weight, and the L-ferritin in the cell was increased to about 95 mg/l after 150 h. In an atomic absorption spectrometry analysis, the intracellular content of iron in the L-ferritin transformant was measured as $1,694{\pm}85\;\mu\textrm{g}g/g$, which is 5.4-fold more than that of the control strain. This L-ferritin transformant could serve as iron-fortified nutrients in animal feed stock.

• Mycobiology
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• v.45 no.3
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• pp.226-231
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• 2017

Coprinopsis cinerea was employed to investigate the fungal response to gravity. Mycelium growth revealed a consistent growth pattern, irrespective of the direction of gravity (i.e., horizontal vs. perpendicular). However, the fruiting body grew in the direction opposite to that of gravity once the primordia had formed. For the proteomic analysis, only curved-stem samples were used. Fifty-one proteins were identified and classified into 13 groups according to function. The major functional groups were hydrolases and transferases (16%), signal transduction (15%), oxidoreductases and isomerases (11%), carbohydrate metabolism (9%), and transport (5%). To the best of our knowledge, this is the first report on a proteomic approach to evaluate the molecular response of C. cinerea to gravity.

• Journal of Korean Public Health Nursing
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• v.18 no.2
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• pp.225-233
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• 2004

The purpose of this study was to measure the sexual life and life satisfaction of elderly in the rural community. For this study, questionnaires were given to 421 elderly enrolled to 16 community health post in Chungcheongbuk-do during a period from November 10, 2003 to January 20, 2004. The results of these analysis are as follows 1. In related to elderly's sexual life, $34.5\%$ of the respondents answered 'very important' or 'important'. $60.2\%$ of the respondents answered they had sexual intercourse. In related to frequency of sexual intercourse, $27.0\%$ of respondents answered they had sexual intercourse over once a month. In related to satisfaction of sexual life, $29\%$ of respondents answered they were satisfied with their sexual life. In related to the sexual life of early age, $66\%$ of respondents answered they had harmonious sexual lives. 2. The mean score of life satisfaction was 2.09 points out of a possible 3 points. Of the field of questionnaire on life satisfaction, the self actualization field showed the highest score. 3. Of the scores related to life satisfaction according to general characteristics, there were statistically significant differences in presence of spouse(p=.001), family pattern(p=.031). personal expenses a month(p=.001), family supporter(p=.013), and health status(p=.000). 4. Of the scores related to life satisfaction according to sexual life, there were statistically significant differences in importance on sexual life(p=.000), performance of sexual life(p=.0011), frequency of sexual life(p=.000), satisfaction of sexual life(p=.000), sexual life of early age(p=.000).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.10
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• pp.4604-4611
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• 2012

The purpose of the research is to investigate the factors affecting the life quality of oral health according to the knowledge and behavior related with oral health. Total 272 people who work in Gyeong-Nam province participated in this survey. The research shows that major 4 factors are subjective whole body health, ages, dietary pattern and dry mouse. The subjective whole body health and dietary pattern have an positive effect on the life quality of oral health, whereas ages and dry mouse an negative effect on this. As a result, the health and the life quality of oral health should be improved by investigating the factors affecting oral health and thus developing the program enhancing the oral health to prevent oral disease.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.95-99
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• 2004

Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG dinucleotides in particular base contexts are known to induce immunity in vertebrate cells. In insect, however, it was recent to find out that ODNs induces insect immunity as other immune inducer such as lipopolysaccharide. However, the finding was solely based on one lepidopteran insect, Bombyx mori, and the expression of insect immunity was neither dependent on numbers of CpG repeats nor methylation of CpG repeats within ODNs. Instead, foreignness of DNA has been suggested to be a key factor governing induction of antibacterial peptide. In this study, we expanded our previous understanding to the potentiality of ODNs as an immune inducer for antifungal peptide in Galleria mellonella and B. mori. To do this, a defensin-type antifungal peptide gene, reported from G. mellonella was cloned and partially sequenced from G. mellonella and B. mori successfully and utilized as a probe in the Northern blot analysis. We found out that ODNs also work as an immune inducer for antifungal peptide in the fat body and midgut of G. mellonella and B. mori larvae. Also, induction pattern of antifungal peptide was irrelevant to the numbers of CpG repeats within ODNs as previously reported on the induction pattern of antibacterial peptides.

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.229-233
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• 2010

Pluripotency and self-renewal capacity of human embryonic stem cells (hESCs) are retained by hESCs related genes as OCT4, SOX2 and NANOG. These genes are shown high expression level in diverse cancer cells and have potential role in the carcinogenesis. On the contrary to this, several genes which are up-regulated in the differentiated hESCs are involved to suppress the carcinogenesis or proliferation of cells. We discovered several genes in immortalized lung fibroblast (WI-38 VA13) by suppression subtractive hybridization. Among them, we focused chromosome 6 open reading frame 62 (C6orf62) which is uncharacterized, mapped to 6p22.3 and generated to Hepatitis B virus X-transactivated proteins (HBVx-transactivated proteins, XTP). Aim of this study was to characterize C6orf62 through analyzing of expression pattern in various cell lines. Expression of C6orf62 was significantly upregulated in diverse normal cell lines than cancer cell lines. And C6orf62 was up-regulated in differentiated hESCs (endothelial cells, neural cells) compared to those of undifferentiated hESCs. Also, C6orf62 in WI-38 cells was highly up-regulated during G1/S transition of the cell cycle. Taken together, C6orf62 is shown expression pattern similar to differentiated hESCs-associated genes which down-regulated in cancer cells. Therefore, we assume that C6orf62 may participate to suppress the proliferation and to induce differentiation through regulating the cell cycle.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.83-92
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• 2015

xBrassicoraphanus line BB#5, a new synthetic intergeneric hybrid between Brassica rapa L. ssp. pekinensis and Raphanus sativus L. var. rafiphera induced by N-methyl-N-nitroso-urethane mutagenesis in microspore culture, shows high seed fertility and morphological uniformity. Dual-color fluorescence in situ hybridization (FISH) using 5S and 45S rDNA probes and genomic in situ hybridization (GISH) using B. rapa genomic DNA probe were carried out to analyze the chromosome composition and the meiosis pairing pattern compared to its parental lines. The somatic chromosome complement is 2n = 38, which consists of 17 metacentric and two submetacentric chromosomes with lengths of 2.18 to $5.01{\mu}m$. FISH karyotype analysis showed five and eight pairs of 5S and 45S rDNA loci. GISH meiosis pairing analysis showed that 19 complete bivalents were most frequent and accounted for 42% of the 100 pollen mother cells examined. Based on chromosome number, size, morphology, rDNA distribution, and meiosis pairing pattern, both parental genomes of B. rapa and R. sativus appear to exist in xBrassicoraphanus line BB#5, demonstrating its genome integrity. Such stable chromosome constitutions and meiotic pairing patterns in somatic and meiotic cells are very rare in natural and synthetic intergeneric hybrids. Chromosomal studies and genetic and phenotypic changes in allopolyploids a re discussed. The results p resented h erein will b e usef ul f or f urther g enomic s tudy o f xBrassicoraphanus lines and their improvement as promising new breeding varieties.

• Journal of East-West Nursing Research
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• v.13 no.2
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• pp.187-196
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• 2007

Purpose: This is a descriptive research to examine the level of health status, depression, and quality of life in the elderly, the relations among the factors. Methods: Subjects were 441 elders in Seoul and Gyung-gi province. Data were collected from July 10, 2006 to October 30, 2006. Measures were CMI, GDS, and SF-36. Data were analyzed by descriptive statistics, Pearson correlation coefficient, T-test, and ANOVA. Results: (1) The health status was the average of 1.75, which indicates being good. Depression was the average of 2.85, which indicate being high, and the quality of life was the average of 2.72, which indicate being moderately. (2) The relations indicate that the better status of health is related with the lower depression, and the better status of health is related with the higher quality of life, and the higher depression is related with the lower quality of life. (3) The health status, depression, and quality of life all showed significant differences according to age, education, past occupation, current occupation, dwelling pattern, monthly pocket money, living expense. Conclusion: for nursing intervention strategies, it are requested the special attention of the current occupation level and interpersonal relation ship in older people.