• Bulletin of the Korean Chemical Society
• /
• v.34 no.2
• /
• pp.460-464
• /
• 2013

Single-chain variable fragments of antibodies (scFv) specific to 2,4-dinitrotoluene (DNT) were isolated from a phage library displaying synthetic human scFv fragments with 6 diversified complementary determining regions (CDRs). A DNT derivative that contained an extended amine group was synthesized and conjugated to the NHS-group that was linked to magnetic beads. Phages specific to the immobilized DNT derivatives were isolated from the library after 4 rounds of sequential binding and elution processes. The displayed scFv fragments from the isolated phages showed consensus CDR sequences. One DNT-specific scFv was expressed in E. coli and purified using Ni-affinity chromatography. The purified DNT-specific scFv binds specifically to the immobilized DNT-derivative with $K_D$ value of $6.0{\times}10^{-7}$ M. The scFv and DNT interaction was not disrupted by the addition of 4-nitrotoluene or benzoic acid. These data demonstrate that the screened scFv from the phage displayed library could be used for selective and sensitive detection of explosives such as TNT.

• The Transactions of the Korea Information Processing Society
• /
• v.4 no.11
• /
• pp.2671-2678
• /
• 1997

The MHEG-5 standard defines representation of multimedia and hypermedia information. The MHEG-5 can make it possible to interchange and present information between heterogeneous networked environment. Also, it can provide real-time user interaction. MHEG-5 object generating is the essential requirements for the MHEG-5 applications, In this paper, we designed encoding/decoding class library for the MHEG-5 objects. We can use it for the interchange on the network. The designed class library can provide base technology for the MHEG-5 based distributed multimedia and hypermedia services on the networked environment.

• Korean Institute of Interior Design Journal
• /
• v.19 no.3
• /
• pp.205-213
• /
• 2010

The importance of communication which is oriented to interaction, fusion and connection is more increased in the post-information society. The information environment is already realized as interface to reach immediately and freely to the databased information and knowledge. The contemporary libraries execute very important roles as an intellectual infra of modern times to deliver the great human achievement and create new culture. In the mean time, the rapid information-oriented society and the development of digital technology bring out big change in the traditional dispatch and receipt method of information. For this reason, libraries as the community space of visitors integrates physical space - electronic space and reconstructs new temporary culture and value to build the new interface that connects human to human, human to space and human to information. On the premise of these awareness, this study analysis the creative interface factor of Tama Art University New Libraries in Ito Toyo in order to research the function and the application method of modern libraries. And the results are follows. 1)We can make sure the significance of communication and the integration consciousness between digital technology and analogue sensitivity that is the characteristic of the post-information society. 2)We can classify for the creative interface factors by the character as space factor(Arcade, Gallery, Lounge, AV Booth, Cafe), facility/equipment factor(Laboratory, Media, Bar, Info Shelf, Media Seat) and furniture factor(Carrel, Desk, Mag Table, XI Shelf). 3)We can see that these interface factors change the stream of library users and that the library is constructed for library users to create and discovery something easily from books and modern technologies. And we can realize also that these factors are creative installation to lead out naturally the latent liberty sense of users.

• Journal of the Korean BIBLIA Society for library and Information Science
• /
• v.29 no.4
• /
• pp.139-160
• /
• 2018

The purpose of this research is to empirically analyze the effect of public library use on the construction of social and cultural capital and the close of social classes reproduction through the survey targeting public library users. As a result, the use of public libraries positively affects the construction of both social and cultural capital. In contrast, it partially addresses the problems in social classes reproduction because the social classes with higher levels of education and income tend to use public libraries more often. In order to take a role of and contribute to equal construction of social and cultural capital, public libraries need to be public places for open and social interaction. In addition, it is necessary for public libraries to improve their roles to allow community members to access and utilize various cultural resources.

• Journal of the Korean Society for information Management
• /
• v.8 no.2
• /
• pp.125-138
• /
• 1991

This paper focuses on issues in the relat~onshlp of library and information science with a closely allied chsciphne, communication studies. It is obvious that researchers in both fields study topics similar in focus, such as information theory, information-seeking behavior includ~ng user studies, systems theory, invisible colleges, the diffusion of knowledge, human interaction with information /communication technologies and the information society. In order to discuss the comparison of theoretical

• Journal of Korean Library and Information Science Society
• /
• v.31 no.3
• /
• pp.89-113
• /
• 2000

The purpose of this study is to establish the new concept of "user-centered" suitable for currentdigital environment and to derive system design factors necessary for user-centered informationretrieval system design of the university libraries.The result of this study can be summarized as follows .First, the concept of 'users-centered' in digital environment where the supporting role ofintermediaries is growing bigger than before in the interaction with remote information retrievalsystem can be defined as the new concept of 'expansion of knowledge'.Second, 6 kinds of system design factors derived for user-centered information retrievalsystem design of the university librariesf the university libraries.

• Journal of Korean Library and Information Science Society
• /
• v.43 no.3
• /
• pp.313-332
• /
• 2012

This study looked at various definitions of reading and examined multiple aspects of reading from the perspective of related disciplines. The study also attempted to isolate specific factors of reading-author, reader, text, and context-and investigated the nature of each factor. The study focused on the communicational aspect of reading, especially on the interaction between the reader and the context.

• Journal of the Korean Magnetic Resonance Society
• /
• v.19 no.3
• /
• pp.132-136
• /
• 2015

Nuclear magnetic resonance (NMR) spectroscopy, owing to its ability to provide atomic level information on molecular structure, dynamics and interaction, has become one of the most powerful methods in early drug discovery where hit finding and hit-to-lead generation are mainly pursued. In recent years, drug discovery programs originating from the fragment-based drug discovery (FBDD) strategies have been widely incorporated into academia and industry in which a wide variety of NMR methods become an indispensable arsenal to elucidate the binding of small molecules onto bimolecular targets. In this review, I briefly describe FBDD and introduce NMR methods mainly used in FBDD campaigns of my company. In addition, quality control of fragment library and practical NMR methods in industrial aspect are discussed shortly.

• Molecules and Cells
• /
• v.19 no.1
• /
• pp.54-59
• /
• 2005

Deoxyribonuclease I (DNase I) is a divalent cation dependent endonuclease and thought to be a significant barrier to effective gene delivery. The only known DNase I-specific inhibitor is monomeric actin which acts by forming a 1:1 complex with DNase I. Its use, however, is restricted because of tendency to polymerize under certain conditions. We screened two random phage peptide libraries of complexity $10^8$ and $10^9$ for DNase I binders as candidates for DNase I inhibitors. A number of DNase I-binding peptide sequences were identified. When these peptides were expressed as fusion proteins with Escherichia coli maltose binding protein, they inhibited the actin-DNase I interaction ($IC_{50}=0.1-0.7{\mu}M$) and DNA degradation by DNase I ($IC_{50}=0.8-8{\mu}M$). Plasmid protection activity in the presence of DNase I was also observed with the fusion proteins. These peptides have the potential to be a useful adjuvant for gene therapy using naked DNA.

• Microbiology and Biotechnology Letters
• /
• v.33 no.4
• /
• pp.255-259
• /
• 2005

IgE-dependent histamine-releasing factor (HRF) is found extracellularly to regulate the degranulation process of histamine in mast cells and basophils and known to play a predominant role in the pathogenesis of chronic allergic disease. HRF has been also identified in the intracellular region of the cell. Previously, we reported that HRF interacts with the 3rd cytoplasmic domain of the alpha subunit of Na,K-ATPase. To understand the molecular mechanism of the regulation of Na, K-ATPase activity by HRF, we investigated the interaction between HRF and TPI since TPI was obtained as HRF-interacting protein in HeLa cDNA library, using yeast two hybrid screening. Domain mapping study of the interaction between HRF and TPI revealed that the C-terminal region of the residue 156-249 of TPI is involved in the interaction with HRF. The interaction between HRF and TPI was confirmed by immunoprecipitation from HeLa cell extracts. Our results suggest that TPI is a HRF-binding protein and the interaction between HRF and TPI nay thus affect Na, K-ATPase activity.