• Journal of the Korean BIBLIA Society for library and Information Science
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• v.35 no.2
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• pp.109-133
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• 2024

This study investigated the impact of the technical characteristics of Generative AI (GAI) and librarians' personal traits on their intention to use GAI. Personalization, interaction, and context awareness were considered as technical characteristics of GAI that influence the intention to use GAI, while innovativeness and frequency of GAI use were considered as librarians' personal traits. The study targeted 187 librarians working in libraries, and 165 questionnaires were collected and analyzed. The results showed that the technical characteristics of GAI had a statistically significant impact on the intention to use GAI. Additionally, librarians' personal traits, namely innovativeness and frequency of GAI use, were also found to have a significant impact on the intention to use GAI. The findings of this study can be used as valuable information to help librarians increase their intention to use GAI and improve the quality and satisfaction of library services.

• BMB Reports
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• v.34 no.1
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• pp.15-20
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• 2001

One of the innate immune reactions in invertebrates is the pro-phenoloxidase (pro-PO) activation system that is involved in the generation of superoxide, melanin synthesis, and the subsequent sequestration of foreign matter entering the hemocoel of the invertebrates. However, the molecular mechanism of this biological reaction is still obscure. To expand our understanding of the biological roles of the pro-PO activation system in invertebrates, we performed a yeast two-hybrid screening by using three regions of pro-PO as bait and a yeast two-hybrid cDNA library from Tenebrio molitor larvae as prey We isolated a novel partial cDNA clone that encodes a glycine-rich protein that interacted with the active phenoloxidase (termed phenoloxidase interacting protein, POIP). POIP consists of two domains: One is an N-terminal unique domain and the other is a C-terminal glycine-rich domain. The C-terminal glycine-rich domain showed sequential homology with those of insect antifungal proteins. Also, the yeast two-hybrid screen in a reverse orientation (using POIP as bait) yielded PO, suggesting that the PO-POIP interaction is specific. By using a 315 bP PCR fragment of the N-terminal unique region of POIP, we cloned the full-length cDNA of POIP from the Tenebruo cDNA library constructed by using E. coli injected larvae. The interaction analysis between PO, and a truncated fragment lacking the N-terminal unique region of POIP, indicated that the N-terminal unique region is necessary for interaction between PO and POIP. The expression level of the POIP mRNA is increased by bacterial injection into T. molitor larvae. This suggests that POIP might be engaged in the humoral defense reaction.

• Parasites, Hosts and Diseases
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• v.46 no.4
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• pp.209-216
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• 2008

A monoclonal antibody against Toxoplasma gondii of Tg556 clone (Tg556) blotted a 29 kDa protein, which was localized in the dense granules of tachyzoites and secreted into the parasitophorous vacuolar membrane (PVM) after infection to host cells. A cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg556, and the full-length was completed by 5'-RACE of 2,086 bp containing an open reading frame (ORF) of 669 bp. The ORF encoded a polypeptide of 222 amino acids homologous to the revised GRA3 but not to the first reported one. The polypeptide has 3 hydrophobic moieties of an N-terminal stop transfer sequence and 2 transmembrane domains (TMD) in posterior half of the sequence, a cytoplasmic localization motif after the second TMD and an endoplasmic reticulum (ER) retrival motif in the C-terminal end, which suggests GRA3 as a type III transmembrane protein. With the ORF of GRA3, yeast two-hybrid assay was performed in HeLa cDNA expression library, which resulted in the interaction of GRA3 with calcium modulating ligand (CAMLG), a type II transmembrane protein of ER. The specific binding of GRA3 and CAMLG was confirmed by glutathione S-transferase (GST) pull-down and immunoprecipitation assays. The localities of fluorescence transfectionally expressed from GRA3 and CAMLG plasmids were overlapped completely in HeLa cell cytoplasm. In immunofluorescence assay, GRA3 and CAMLG were shown to be co-localized in the PVM of host cells. Structural binding of PVM-inserted GRA3 to CAMLG of ER suggested the receptor-ligand of ER recruitment to PVM during the parasitism of T. gondii.

• Journal of Life Science
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• v.23 no.11
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• pp.1311-1316
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• 2013

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a major pre-mRNA binding protein and it is an abundant nuclear protein that shuttles between the nucleus and the cytoplasm. hnRNP L is known to be related to many cellular processes, including chromatin modification, pre-mRNA splicing, mRNA export of intronless genes, internal ribosomal entry site (IRES)-mediated translation, mRNA stability, and spermatogenesis. In order to identify the cellular proteins interacting with hnRNP L, this study performed a yeast two-hybrid screening, using a human liver cDNA library. The study identified insulin-like growth factor binding protein-4 (IGFBP-4) as a novel interaction partner of hnRNP L in the human liver. It then discovered, for the first time, that hnRNP L interacts specifically with IGFBP-4 in a yeast two-hybrid system. The authenticity of this two-hybrid interaction of hnRNP L and IGFBP-4 was confirmed by an in vitro pull-down assay.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.22 no.3
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• pp.189-206
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• 2011

This paper proposes a conceptual framework to explore the ways in which people work with in accessing, sharing, and navigating Web resources. In order to provide a better frame of a user's interaction with a folksonomy, an information foraging approach was adapted that denotes adaptive information seeking behaviors of users within human information interaction. A conceptual framework that consists of three different components from users' points of view was proposed: tagging, navigation, and knowledge sharing. This understanding will help us to motivate possible future directions of research in folksonomy and lay the groundwork for empirical research which focuses on qualitative analysis of a folksonomic and users' tagging behaviors.

• Journal of the Korean Society for Library and Information Science
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• v.41 no.4
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• pp.217-227
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• 2007

This study describes a system which makes children use a cooperation study for the education of a library use and applies SDG technology to this cooperation study SDG means a system which is able to do a cooperation work as a multi-inputting device in a computer display. Through the use library utilization education system based on SDG, learners study simultaneously and collaborately. On this thesis. We embodied the understanding of the decimal classification of Korea and the arrangement of a bookshelf and the study of the location tracking as a game style based on an existed study that a cooperation study used SDG is more superior that an individual study used a single device. Librarians through this system will be able to easily and interestingly instruct children for the use of a library.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.29 no.1
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• pp.185-208
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• 2018

This study has been made under the purpose of revealing the real effectiveness of Library Application Education, Life and Career Education and Reading Education in order to seek for the activation of school library and right roles of librarian teachers and subject teachers through connected to school library educations according to the free-semester system. For that, the survey was made to 73 teachers and 112 students, who already experienced connected library educations. As a result, the most frequent educations made by teachers and students was Reading Education, the second one was Library Application Education and the least one was Life and Career Education. In the aspect of effectiveness in teachers and students, the result came out quite meaningful and for teachers, especially the manner in the field of expertism, the satisfaction level of teachers was high in Life and Career Education in format style and creativity in source materials. For the students, the meaningful effectiveness shows in interaction field. Based on the research: first, study about various models of Reading Education which will be applied in connected to school library education according to the free-semester system. Second, additional researches are necessary for the expansional aspect of Library Application Education, Life and Career Education and Reading. Third, continuous studies are essential for the elements which can contribute effectiveness. Fourth, offering many strategical methods are essential to demonstrate creativity for the increase in satisfaction level for teachers.

• Journal of Korean Library and Information Science Society
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• v.55 no.2
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• pp.29-53
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• 2024

Public library programs serve a cultural function and are a tool for community interaction. Recently, there has been an increase in the number of websites that integrate the programs of various institutions, but there is no set framework to describe the programs. Therefore, in order to prepare a framework for program information, we conducted a content analysis of Jeongdok Library programs as an example. Using MAXQDA, a content analysis tool, category codes for type, topic, special classification, and target audience were derived. Based on this, we analyzed the characteristics of the Jeongdok library programs as follows. In terms of type, there are many programs such as classes and lectures, but fewer programs related to tours, performances and screenings, and operational experiences. In terms of topic matter, programs related to reading and the arts were dominant, while programs related to book curation, awards, and the environment were less common. In terms of target audience, the most common programs were for adults, with fewer programs for high school students, middle school students, and library staff. The framework of Jeongdok library program can be used not only to classify the programs currently operated by other public libraries, but also to develop a service platform for public library programs in Korea.

• Journal of Korean Library and Information Science Society
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• v.54 no.2
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• pp.131-154
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• 2023

This study analyzed the effectiveness of visiting reading programs for vulnerable children, focusing on the case of libraries in A-si, Gyeonggi-do. Library A utilized a visiting teacher to deliver books that matched the children's levels and interests through non-face-to-face interactions, or to engage in face-to-face reading sessions and deliver books together. In 2022, a total of 158 individuals from 103 families participated in the program, with 103 individuals from 67 families delivering books and 55 individuals from 36 families engaging in book reading activities. Pre- and post-project surveys were conducted to assess the reading effects, and interviews were conducted with the visiting teachers. The analysis revealed that the level and interest of the children during book delivery and reading sessions were identified, and the effects of reading books with high levels of interaction were significant. Infants who were able to easily acquire reading experiences according to their age exhibited better reading effects compared to elementary school students. The program aimed at the new participant group showed slightly greater effectiveness than the group of continuing participants. Furthermore, following the implementation of the program, improvements were observed in reading interest, reading attitude, reading environment, reading interaction, self-esteem, social skills, language ability, reading proficiency, self-directed learning ability, and library utilization rate.

• Journal of the Korean Society for information Management
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• v.19 no.4
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• pp.113-135
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• 2002

An interface or integration among various information system is needed to construct knowledge management infrastructure. Particularly, digital library and knowledge management system have to be implemented in the view of integration for activating interaction of organizational knowledge. So this study conducts comparative analysis between two systems in the perspective of knowledge management key factors, and then explores the ways toward an integration model. The integration model is designed for activating user interface and consistent flow of knowledge. This model is expected to contribute to increase the performance of knowledge management.