• BMB Reports
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• 제34권6호
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• pp.526-530
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• 2001

The translationally controlled tumor protein (TCTP), also known as the IgE-dependent histamine releasing factor (HRF), was used in the yeast two-hybrid system to screen the interacting molecules. We obtained the N-terminus truncated rat fast myosin alkai light chain from the rat skeletal muscle cDNA library in the screening. Since either TCTP/HRF or the myosin light chain is known to be associated with histamine secretion from RBL-2H3 cells, we investigated the possible interaction between rat TCTP/HRF and nonmuscle myosin light chain in these cells. We used affinity chromatography and coimmunoprecipitation. Our data suggests that HRF and the myosin light chain interact, which may play an important role in histamine release in RBL-2H3 cells.

• BMB Reports
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• 제33권1호
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• pp.59-62
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• 2000

Hepatitis C virus (HCV) nonstructural 5B (NS5B) protein is an RNA-dependent RNA polymerase (RdRp). To determine whether it can contribute to viral replication by interaction with cellular proteins, the yeast two-hybrid screening system was employed to screen a human liver cDNA library. Using the HCV NS5B as a bait, we have isolated positive clones encoding a cellular protein. The NS5B interacting protein, 5BIP, is a novel cellular protein of 170 amino acids. Interaction of the HCV NS5B protein with 5BIP was confirmed by a protein-protein blotting assay. Recently, we have demonstrated that NS5B possesses an RdRp activity and thus it is possible that 5BIP, in association with NS5B, plays a role in HCV replication.

• 정보관리연구
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• 제33권2호
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• pp.1-17
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• 2002

본 연구는 이용자 인터페이스 설계 원칙과 평가방법에 관하여 고찰하고 관련되는 사례를 검토하였다. 제품 개발에 중요한 이용자 인터페이스 설계 원칙으로 1) 이용자와 작업 중심의 설계, 2) 기능성 중심의 설계, 3) 이용자 관점에서 설계, 4) 이용자가 작업 수행을 간단, 명료하게 진행하도록 설계, 5) 배우기 쉬운 인터페이스 설계, 6) 단순 데이터가 아닌 정보를 전달하는 인터페이스 설계, 7) 적절한 피드백을 제공하는 인터페이스 설계, 8) 이용자 테스트를 통한 설계보완 등이 있다. 또한 이 논문은 설계 원칙과 평가방법을 소개하기 위한 기초조사로 인간-컴퓨터 상호작용과 관련된 문제들에 대해서도 기술하였다.

• Genomics & Informatics
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• 제21권3호
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• pp.41.1-41.11
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• 2023

The Dengue virus M protein is a 75 amino acid polypeptide with two helical transmembranes (TM). The TM domain oligomerizes to form an ion channel, facilitating viral release from the host cells. The M protein has a critical role in the virus entry and life cycle, making it a potent drug target. The oligomerization of the monomeric protein was studied using ab initio modeling and molecular dynamics simulation in an implicit membrane environment. The representative structures obtained showed pentamer as the most stable oligomeric state, resembling an ion channel. Glutamic acid, threonine, serine, tryptophan, alanine, isoleucine form the pore-lining residues of the pentameric channel, conferring an overall negative charge to the channel with approximate length of 51.9 Å. Residue interaction analysis for M protein shows that Ala94, Leu95, Ser112, Glu124, and Phe155 are the central hub residues representing the physicochemical interactions between domains. The virtual screening with 165 different ion channel inhibitors from the ion channel library shows monovalent ion channel blockers, namely lumacaftor, glipizide, gliquidone, glisoxepide, and azelnidipine to be the inhibitors with high docking scores. Understanding the three-dimensional structure of M protein will help design therapeutics and vaccines for Dengue infection.

• The Plant Pathology Journal
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• 제30권1호
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• pp.58-67
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• 2014

The multifunctional triple gene block protein 1 (TGB1) of the Potexvirus Alternanthera mosaic virus (AltMV) has been reported to have silencing suppressor, cell-to-cell movement, and helicase functions. Yeast two hybrid screening using an Arabidopsis thaliana cDNA library with TGB1 as bait, and co-purification with TGB1 inclusion bodies identified several host proteins which interact with AltMV TGB1. Host protein interactions with TGB1 were confirmed by biomolecular fluorescence complementation, which showed positive TGB1 interaction with mitochondrial ATP synthase delta' chain subunit (ATP synthase delta'), light harvesting chlorophyll-protein complex I subunit A4 (LHCA4), chlorophyll a/b binding protein 1 (LHB1B2), chloroplast-localized IscA-like protein (ATCPISCA), and chloroplast ${\beta}$-ATPase. However, chloroplast ${\beta}$-ATPase interacts only with $TGB1_{L88}$, and not with weak silencing suppressor $TGB1_{L88}$. This selective interaction indicates that chloroplast ${\beta}$-ATPase is not required for AltMV movement and replication; however, TRV silencing of chloroplast ${\beta}$-ATPase in Nicotiana benthamiana induced severe tissue necrosis when plants were infected by AltMV $TGB1_{L88}$ but not AltMV $TGB1_{L88}$, suggesting that ${\beta}$-ATPase selectively responded to $TGB1_{L88}$ to induce defense responses.

• 한국문헌정보학회지
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• 제12권
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• pp.269-327
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• 1985

This study was undertaken to meet more fully the demands for improved training of library personnel, occasioned by the rapidly changing roles and functions of libraries as they try to adapt to the vast social, economic and technological changes currently in progress in the Korean society. The specific purpose of this research is to develop a standard curriculum at the batchelor's level that will properly equip the professional personnel in Korean libraries for the changes confronting them. This study started with the premise that to establish a sound base for curriculum development, it was necessary first to determine what concepts, knowledge, and techniques are required for professional library personnel to perform it at an optimal level of efficiency. Explicitly, it was felt that for the development of useful curricula and courses at the batchelor's level, a prime source of knowledge should be functional behaviours that are necessary in the job situation. To determine specifically what these terminal performance behaviours should be so that learning experience provided could be rooted in reality, the decision was reached to use a systems approach to curriculum development, which is an attempt to break the mold of traditional concepts and to approach interaction from an open, innovative, and product-oriented perspective. This study was designed to: (1) identify what knowledge and techniques are required for professional library personnel to perform the job activities in which they are actually engaged, (2) to evaluate the educational needs of the knowledge and techniques that the professional librarian respondents indicate, and (3) to categorise the knowledge and techniques into teaching subjects to present the teaching subjects by their educational importance. The main data-gathering instrument for the study, a questionnaire containing 254 items, was sent to a randomly selected sample of library school graduates working in libraries and related institutions in Korea. Eighty-three librarians completed and returned the questionnaire. After analysing the returned questionnaire, the following conclusions have been reached: (A) To develop a rational curriculum rooted in the real situation of the Korean libraries, compulsory subjects should be properly chosen from those which were ranked highest in importance by the respondents. Characters and educational policies of, and other teaching subjects offered by, the individual educational institution to which a given library school belongs should also be taken into account in determining compulsory subjects. (B) It is traditionally assumed that education in librarianship should be more concerned with theoretical foundations on which any solution can be developed than with professional needs with particulars and techniques as they are used in existing library environments. However, the respondents gave the former a surprisingly lower rating. The traditional assumption must be reviewed. (C) It is universally accepted in developing library school curricula that compulsory subjects are concerned with the area of knowledge students generally need to learn and optional subjects are concerned with the area to be needed to only those who need it. Now that there is no such clear demarcation line provided in librarianship, it may be a realistic approach to designate subjects in the area rated high by the respondents as compulsory and to designate those in the area rated low as optional. (D) Optional subjects that were ranked considerably higher in importance by the respondents should be given more credits than others, and those ranked lower might be given less credits or offered infrequently or combined. (E) A standard list of compulsory and optional subjects with weekly teaching hours for a Korean library school is presented in the fourth chapter of this report.

• 한국산업융합학회 논문집
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• 제6권3호
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• pp.207-214
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• 2003

Children of three-to-eighteen-years-old have been brought up in the institution (Orphanage) for several reasons such as family dissolution, divorce and poverty since the Korean Was of 1950. The Cottage, where these children are housed and brought up has dining room, library and bedrooms, plays as a significant social interaction place as well as study. Since the most facilities were built during the 1950-1960s period, most of the cottages are now in the condition of being rebuilt or remodelled. Because living space and studying space are mixed, it will be necessary to provide another studying space separated from living space. And then it will be also necessary to provide a space for play or rest between the two spaces. This study was conducted to obtain some basic data necessary for design of spaces in the orphanages by examining the relations between the studying activities of children and residence types.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2014년도 하계학술대회
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• pp.183-184
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• 2014

최근 스마트 폰 가입자 수 증가와 함께 모바일 기기의 보급이 대중화 되었다. 모바일 기기 대중화로 다양한 형태의 멀티미디어 콘텐츠 소비가 많아지면서 새로운 콘텐츠를 요구하는 사용자들 늘어나고 있다. 다양한 소비 계층에서 콘텐츠의 수요가 늘어나고 있지만, 현재 시장에서는 공급량이 수요에 미치지 못하고 있다. 모바일 기기중 하나인 디지털 앱북 콘텐츠 또한 시장에서 더 많은 콘텐츠가 필요하다. 본 논문에서는 디지털 앱북의 콘텐츠 증가를 위해 디지털 앱북 제작에 있어 코드 재사용성 증가로 인한 체계적인 개발과 유지지보수의 용이함을 높이기 위해 사용자와의 다양한 상호작용 기능들을 라이브러리로 구현하고 검증하였다. 라이브러리는 사용자 동작부와 기기 동작부로 구성하고 각 동작부의 명령들을 조합하여 상호작용 메소드 형태로 존재하도록 설계하였다. 구현된 라이브러리는 코드 사용량을 크게 줄여 개발 시간 단축에 영향을 주어 디지털 앱북 콘텐츠 제작에 있어 효율을 높여준다.

• 미생물과산업
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• 제14권1호
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• pp.17-20
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• 1988

A genetic analysis of the complex Bacillus subtilis transcriptional apparatus is essential to understand the function, regulation, and interaction of the transcriptase components during growth and sporulation. This approach in Escherichia coli has uncovered fundamental mechanisms regulating gene expression Cole and Nomura, 1986; Lindahl and Zengel, 1986) and an analysis of the B. subtilis transcriptase will allow comoparison of the E.coli system to another bacterium that has evolved under different selective pressures. To this end we used antibody probes to isolate the alpha, beta, and beta' core subunit genes from a .lambda.gtill expression vector library. To address the question of function ans regulation of the minor sigma factors that confer promoter specifity on the polymerase core (Losick et al., 1986), we used the same approach to isolate the gene for the 37,000 dalton sigma factor, sigma-37.

• 한국연소학회지
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• 제4권2호
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• pp.51-62
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• 1999

NOx formation in turbulent flames is strongly coupled with temperature, superequilibrium concentration of O radical, and residence time. This implies that in order to accurately predict NO level, it is necessary to develop sophisticated models able to account for the complex turbulent combustion processes including turbulence/chemistry interaction and radiative heat transfer. The present study numerically investigates the turbulent nonpremixed hydrogen jet flames using the laminar flamelet model. Flamelet library is constructed by solving the modified Peters equations and the turbulent combustion model is extended to nonadiabatic flame by introducing the enthalpy defect. The effects of turbulent fluctuation are taken into account by the presumed joint PDFs for mixture fraction, scalar dissipation rate, and enthalpy defect. The predictive capability of the present model has been validated against the detailed experimental data. Effects of nonequilibrium chemistry and radiative heat loss on the thermal NO formation are discussed in detail.