• BMB Reports
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• v.29 no.6
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• pp.519-524
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• 1996

Peptidylprolyl cis-trans isomerase (PPlase) catalyzes the cis-trans isomerization of prolyl peptide and facilitates the folding of cellular proteins and peptides. PPlase consists of two distinct immunophilins, each specifically binding to the immunosupressive drug cyclosporin A (CsA) or FK506, respectively. A PPlase was isolated and partially purified from porcine spleen. The molecular weight of porcine spleen PPlase was determined to be ~14,000 on the basis of SDS-PAGE. The purified enzyme was strongly inhibited by FK506, but not by CsA. The inhibition constant and the true concentration of enzyme preparations were determined by active site titration using the tight binding inhibitor FK506: $K_{i}=18.7$ nM and $E_{t}=172$ nM. The equilibrium ratio of conformer. [cis]/[trans], of prolyl peptide substrates (N-Suc-Ala-Xaa-Pro-Phe-p-NA) in anhydrous trifluoroethanol/LiCl solvent system varied from 0.24 to 0.85 depending on the nature of Xaa. Overall. in this solvent-salt system, the populations of the cis conformer of substrates in equilibrium are higher than in an aqueous solution so that the substantial error caused by high background absorption can be reduced. The reactivities of porcine spleen PPlase are shown to be highly sensitive to changes in the structure of substrates. Thus, $k_{cat}/K_m$ value for the most reactive substrate (Xaa Leu) is $4.007+10^{6}M^{1}s^{1}$ and, is 2,636 fold higher than that for the least reactive peptide substrate tested, Xaa=Glu.

• Proceedings of the SAREK Conference
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• 2008.11a
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• pp.203-211
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• 2008

The optimal design of two stage evaporation & absorption system which is related to the large temperature difference system was investigated numerically in the absorption refrigeration system. The concentrations at inlet & oulet of absorber are 62.9% and 56.9%, but in two stage absorption system the values are 62.2% and 56.2%. Therefore strong solution & weak solution became diluted than the standard value. The amount of weak solution circulation can be reduced in absorption refrigeration system, and the sensible heat load is more reduced to enhance the COP of system. As UAR is increased, COP becomes larger, and this means the role of top section is more important than bottom section in two stage evaporation & absorption system. But the increase of COP becomes slower at 0.7 of UAR ratio. The performance of Type2 is higher than Type1 in COP with the flow direction of cooling waters. This phenomena is due to the active absorption of vapor -absorption & lower temp. cooling water is more effective. The pressure at bottom section becomes higher & that at top section becomes lower and therefore the circulation rate can be diminished more.

• The Journal of the Korea institute of electronic communication sciences
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• v.13 no.5
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• pp.917-922
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• 2018

In the conventional interchannel interference self-cancellation (ICI-SC) schemes, the length of sampling window is the same as the symbol length of orthogonal frequency division multiplexing (OFDM). Thus, the number of complex operations to compute the interference coefficient of each subchannel is significantly increased. To solve this problem, we present an approximated mathematical model for the coefficients of ICI-SC schemes. Based on the proposed approximation, we analyze mean squared error (MSE) and computational complexity of the ICI-SC schemes with the length of sampling window. As a result, the presented approximation has an error of less than 0.01% on the MSE compared to the original equation. When the number of subchannels is 1024, the number of complex computations for the interference coefficients is reduced by 98% or more. Since the computational complexity can be remarkably reduced without sacrificing the self-cancellation capability, it is considered that the proposed approximation is very useful to develop an algorithm for the ICI-SC scheme.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.1
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• pp.127-132
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• 2006

The Global Positioning System (GPS), Inertial Navigation System (INS) and Pseudolite (PL) technologies all play very important roles in navigation systems. As an independent navigation system, GPS can provide high precision positioning results which are independent of time. However, the performance will become unreliable when the system experiences high dynamics, or when the receiver is exposed to jamming or RF interference. In comparison to GPS, though INS is autonomous and provides good short-term accuracy, its use as a standalone navigation system is limited due to the time-dependent growth of the inertial sensor errors. PLs are ground-based transmitters that can transmit GPS-like signals. They have some advantages in that their positions can be determined precisely, and the Signal-to-Noise Ratios (SNR) are relatively high. Because their combined performance, in principle, overcomes the shortcomings of the individual systems, the integration of GPS, INS and PL is increasingly receiving attention from researchers. Depending on the desired performance vs complexity, system integration can be carried out at different levels, namely loose, tight and ultra-tight coupling. Compared with loose and tight integration, although it is more complex in terms of system design, ultra-tight integration will be the basis of the next generation of reliable and robust navigation systems. Its main advantages include improved performance under exposure to high dynamics, and jamming and RF interference mitigation. This paper presents an overview of the ultra-tight integration developments and discusses some of the challenging issues.

• Journal of Biosystems Engineering
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• v.41 no.4
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• pp.408-417
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• 2016

Purpose: Yield monitoring systems are an essential component of precision agriculture. They indicate the spatial variability of crop yield in fields, and have become an important factor in modern harvesters. The objective of this paper was to review research trends related to yield monitoring sensors for grain crops. Methods: The literature was reviewed for research on the major sensing components of grain yield monitoring systems. These major components included grain flow sensors, moisture content sensors, and cutting width sensors. Sensors were classified by sensing principle and type, and their performance was also reviewed. Results: The main targeted harvesting grain crops were rice, wheat, corn, barley, and grain sorghum. Grain flow sensors were classified into mass flow and volume flow methods. Mass flow sensors were mounted primarily at the clean grain elevator head or under the grain tank, and volume flow sensors were mounted at the head or in the middle of the elevator. Mass flow methods used weighing, force impact, and radiometric approaches, some of which resulted in measurement error levels lower than 5% ($R^2=0.99$). Volume flow methods included paddle wheel type and optical type, and in the best cases produced error levels lower than 3%. Grain moisture content sensing was in many cases achieved using capacitive modules. In some cases, errors were lower than 1%. Cutting width was measured by ultrasonic distance sensors mounted at both sides of the header dividers, and the errors were in some cases lower than 5%. Conclusions: The design and fabrication of an integrated yield monitoring system for a target crop would be affected by the selection of a sensing approach, as well as the layout and mounting of the sensors. For accurate estimation of yield, signal processing and correction measures should be also implemented.

• The Journal of Sustainable Design and Educational Environment Research
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• v.18 no.3
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• pp.39-49
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• 2019

Confucian ideologies formed a unique spatial order in the spatial composition of seowon architecture. Confucian scholars viewed ancient China as the ideal society, so they tried to apply the "Myochim" theory, the architectural system of the time, to the Joseon society. However, it was not easy to apply ancient architectural ideas in Joseon in the 16th century. Therefore, various opinions were presented by scholars to establish the relationship between Joseon alone. Unlike the Ki-ho school application of this theory, the construction of Seowon was done according to ancient principles from a practical standpoint, there were fewer experiments for actual application in Yeong-nam school. This was a pragmatic attitude that presupposes a clear difference between the ancient Chinese system and the reality of Joseon in the 17th century.

• Smart Structures and Systems
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• v.15 no.1
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• pp.99-117
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• 2015

The Stonecutters Bridge (SCB) in Hong Kong is the third-longest cable-stayed bridge in the world with a main span stretching 1,018 m between two 298 m high single-leg tapering composite towers. A Wind and Structural Health Monitoring System (WASHMS) is being implemented on SCB by the Highways Department of The Hong Kong SAR Government, and the SCB-WASHMS is composed of more than 1,300 sensors in 15 types. In order to establish a linkage between structural health monitoring and maintenance management, a Structural Health Rating System (SHRS) with relevant rating tools and indices is devised. On the basis of a 3D space frame finite element model (FEM) of SCB and model updating, this paper presents the development of an SHR-oriented 3D multi-scale FEM for the purpose of load-resistance analysis and damage evaluation in structural element level, including modeling, refinement and validation of the multi-scale FEM. The refined 3D structural segments at deck and towers are established in critical segment positions corresponding to maximum cable forces. The components in the critical segment region are modeled as a full 3D FEM and fitted into the 3D space frame FEM. The boundary conditions between beam and shell elements are performed conforming to equivalent stiffness, effective mass and compatibility of deformation. The 3D multi-scale FEM is verified by the in-situ measured dynamic characteristics and static response. A good agreement between the FEM and measurement results indicates that the 3D multi-scale FEM is precise and efficient for WASHMS and SHRS of SCB. In addition, stress distribution and concentration of the critical segments in the 3D multi-scale FEM under temperature loads, static wind loads and equivalent seismic loads are investigated. Stress concentration elements under equivalent seismic loads exist in the anchor zone in steel/concrete beam and the anchor plate edge in steel anchor box of the towers.

• Integrative Medicine Research
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• v.7 no.1
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• pp.95-102
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• 2018

Background: Hypertension is a major cause of cardiovascular disease and associated mortality, and postmenopausal women are at a high risk of hypertension. We aim to investigate the hypotensive effect and safety of acupuncture, focusing on postmenopausal women with prehypertension and stage 1 hypertension. In addition, we aim to investigate whether the effect of acupuncture treatment differed, depending on Sasang Constitution and cold-heat pattern. Methods: This study is designed as an intervention cohort study. Two hundred postmenopausal women aged <65 years with prehypertension or stage 1 hypertension living in Daejeon city in Korea will be recruited, and randomly assigned to either an acupuncture or no-treatment control group. The intervention will consist of four sessions; one session will include acupuncture performed 10 times for 4 weeks. There will be a 20-week observation period after each session, and the total study duration will be 96 weeks. Acupuncture will be applied at the bilateral Fengchi (GB20), Quchi (LI11), Zusanli (ST36), and Sameumgyo (SP6) acupoints. The effect of acupuncture will be evaluated by comparing the change in systolic and diastolic blood pressure between the acupuncture and control groups every 4 weeks until the end of the study. Discussion: To evaluate the success of blood pressure management, long-term observation is required, but no long-term studies have been conducted to evaluate the effect of acupuncture on blood pressure in postmenopausal women. To our knowledge, this study will be the first long-term study to investigate this issue for more than 6-8 weeks.

• Journal of Microbiology and Biotechnology
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• v.31 no.8
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• pp.1144-1153
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• 2021

A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp. mesenteroides. The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 10⁵ Da. Analysis of the monosaccharide composition revealed that the molecule was mainly composed of glucose. The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds. ¹H and ¹³C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid. Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures. In addition, rEPS414 exhibited strong in vitro antioxidant activity. These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.321-321
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• 2018

태풍은 단시간동안 인류활동에 막대한 영향력을 미치는 저기압 시스템 중 하나로 인류에 중요한 수자원을 공급하여 물 부족 현상을 해소하거나, 열대해역에 축적된 과잉 에너지를 수송하여 에너지 균형을 유지하고, 해양 및 대기 정화로 인한 생태계를 활성화하는 긍정적인 면과 육지에 상륙함에 따라 심한 강풍과 폭우를 동반하여 인적 경제적 피해를 주는 부정적인 면을 동시에 지니고 있는 중요한 대기현상이다(Lee, 2006; Wang, 2010; Xu, 2013; Delphine et al., 2013). 2002년 루사, 2003년 매미, 2012년 볼라벤, 2013년 하이옌 그리고 2016년 차바와 같이 세력이 강한 태풍으로 인해 동아시아 지역은 매년 막대한 인명 및 재산피해가 야기되고 있는 실정임에 따라, 태풍으로 인한 재해를 최소화하고 태풍이 가지는 긍정적인 혜택을 적극 활용하기 위해서는 태풍의 활동 및 호우 예측 및 경향성에 대해 보다 정량적인 연구가 필요하다(Oh et al., 2011; Kim and Jain, 2011; Li and Zhou, 2012; Son et al., 2013). 따라서, 본 연구에서는 Son et al.(2014)이 제시한 태풍의 경로 및 규모를 고려한 태풍 추출기법을 적용하여 한반도에 영향을 미친 태풍 및 태풍강우를 추출하였으며, 이를 과거 1977-1994년(Period 1)과 1995-2012년(Period 2) 두 기간으로 분류하였다. 또한, 태풍의 최대풍속을 기준으로 3단계(35-64kt, 65-94kt, 95kt이상)로 구분하여 과거 시기별 태풍 활동(최대풍속, 최소중심기압, 발생빈도, 발생지점, 전향점, 경로) 특성을 분석하고, 과거 시기별 태풍강우(시간 최대 태풍강우량, 총 태풍 강우량)의 변화 분석을 수행하였다. 본 연구의 결과는 향후, 태풍과 관련하여 수질개선 및 수자원확보 등과 같은 태풍 활용방안과 태풍 피해저감을 위한 치수대책 수립 등에 대한 기초자료로 활용가능하며, 기후변화로 인해 야기될 수 있는 태풍의 활동 및 강우 특성 변화를 파악하는 데 이용가능 할 것으로 판단된다.