• Journal of Nutrition and Health
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• 제25권1호
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• pp.12-21
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• 1992

This study was aimed to investigate the relations between self-selected intake of 3 macronutrie-nts and body weight gain and body fat accumulation in male rats given three isocaloric diets differing carbohydrate protein and fat contents concurrently. Also the effect of dietary restriction was observed. Forty two male rats of Sprague-Dawley strain weighing 68.7$\pm$6.1g were randomly divided into 2 groups and were allowed to have foods from 3 different cups for 8-hours a day or ad libitum, After 12 weeksthey were decapitated and their brains were quickly removed and frozen until they were assayed for serotonin and its metabolite 5-HIAA The carcass was dried at 105$\pm$2$^{\circ}C$ and measured the contents of body water and body fat. The animals chose a moderately high and constant carbohydrate level and showed the increase of percent protein intake with age and great individual variations. Protein in the diet seemed to trigger appetite and increase food intake which resulted in higher weight gains and in more fat deposition in the body. The concentration of brain serotonin did not show any correlations with the intake of nutrients. the accumulation of body fat and the gain of body weight.

• Journal of Oral Medicine and Pain
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• 제24권3호
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• pp.269-280
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• 1999

구강안면동통환자의 발통점에 대한 보존적 치료방법중 저출력레이저의 효과를 평가하기 위해 교근, 측두근과 승모근에 발통점을 가진 치과대학생 69명을 무작위로 분류하여 37명에게는 GaAlAs 반도체 레이저를 조사하였고 나머지 32명은 레이저를 실제로 조사하지 않고 대조군으로 삼았다. 50mW, 820nm의 GaAlAs 반도체 레이저를 이용하여, 4주 동안 첫 주는 2회, 이후 3주 동안 1회씩 총 5회 조사하였고 전자통각계를 이용하여 압력통각역치를 측정한 후 이를 대조군과 비교한 바 다음과 같은 결과를 얻었다. 1. 남녀 및 조사군과 비조사군의 치료 전 압력통각역치는 차이가 없었다. 2. 조사군의 각 근육에서 측정한 압력통각역치는 레이저 치료 2주 후부터 유의하게 높아졌으나 비조사군에서는 차이가 없었다, 3. 비조사군의 치료 전, 후 압력통각역치에는 성 차가 없었다. 반면 종사군의 압력통각역치는 치료 전에는 성 차가 없었으나 치료 후에는 남성이 여성보다 유의하게 높았다.

• Clinical and Experimental Vaccine Research
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• 제13권3호
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• pp.232-241
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• 2024

Purpose: Brucellosis, a zoonotic infectious disease, is a worldwide health issue affecting animals and humans. No effective human vaccine and the complications caused by the use of animal vaccines are among the factors that have prevented the eradication of the disease worldwide. However, bio-engineering technologies have paved the way for designing new targeted and highly efficacious vaccines. In this regard, the study aimed to evaluate immunity induced by mannosylated niosome containing Brucella recombinant trigger factor/Bp26/Omp31 (rTBO) chimeric protein in a mouse model. Materials and Methods: rTBO as chimeric antigen (Ag) was expressed in Escherichia coli BL21 (DE3) and, after purification, loaded on niosome and mannosylated niosome. The characteristics of the nanoparticles were assessed. The mice were immunized using rTBO, niosome, and mannosylated niosome-rTBO in intranasal and intraperitoneal routes. Serum antibodies (immunoglobulin [Ig]A, IgG, IgG1, and IgG2a) and splenocyte cytokines (interferon-gamma, interleukin [IL]-4, and IL-12) were evaluated in immunized mice. Finally, immunized mice were challenged by B. melitensis and B. abortus. A high antibody level was produced by niosomal antigen (Nio-Ag) and mannosylated noisomal antigen (Nio-Man-Ag) compared to the control after 10, 24, and 38 days of immunization. The IgG2a/IgG1 titer ratio for Nio-Man-Ag was 1.2 and 1.1 in intraperitoneal and intranasal methods and lower than one in free Ag and Nio-Ag. Cytokine production was significantly higher in the immunized animal with Ag-loaded nanoparticles than in the negative control group (p<0.05). Moreover, cytokine and antibody levels were significantly higher in the injection than in the inhalation method (p<0.05). Results: The combination of mannosylated noisome and rTBO chimeric proteins stimulate the cellular and humoral immune response and produce cytokines, playing a role in developing the protective acquired immune response in the Brucella infectious model. Also, the intraperitoneal route resulted in a successful enhancement of cytokines production more than intranasal administration. Conclusion: Designing an effective vaccine candidate against Brucella that selectively induces cellular and humoral immune response can be done by selecting a suitable nanoniosome formulation as an immunoadjuvant and recombinant protein as an immune response-stimulating Ag.

• 대한수의학회지
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• 제43권2호
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• pp.211-218
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• 2003

Human ovarian cancerous cells survive in a way that they trigger the nucleotide excision repair (NER) or double-strand DNA repair (dsDNA) repair mechanism to show resistance to anticancer drugs and activate many kinds of repair protein, thus removing damaged DNAs. Two experiments on the PA-1 human ovarian teratocareinoma cell line that hardly has any expression of epidermal growth factor receptor (EGFR) were conducted in the study; first, EGF-R was transfected and its receptor was obtained. The receptor was investigated in terms of its mutual relations with many kinds of protein concerning NER or dsDNA repair. Second, it was examined what kind impact cisplatin and adriamycin had on the effects of EGF-R over the PA-1 cell line lacking EGF-R. When being administered with cisplatin and adriamycin, Hey and Hey C2 cell lines showed a high level of resistance while PA-1 cell line a high level of sensitivity. Hey and Hey C2 cell lines that are resistant against anticancer drugs exhibited a high level of EGF-R expression while PA-1 cell line that is sensitive to them did a much lower level of the expression. When PA-1 cell line was transfected for the expression of DNA adduct and EGF-R, it showed a higher level of resistance compared to the control group. There was no difference in the expression of DNA repair proteins (DNA- dependent protein kinase, Ku70, and Ku80) between Hey and the PA-1 cell lines. The results indicate that the Hey cell line that is resistant against cisplatin and adriamycin works along the signaling system responding to the changes of EGF-R while the PA-1 cell line that is sensitive to both of them does to the lack of EGF-R.

• 한국동물학회지
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• 제30권1호
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• pp.1-9
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• 1987

생쥐 난자-난구 복합체를 인공배양하면서 adenylate cyclase의 촉진제인 forskilin과 phosphodiesterase의 저해제인 3-isobutyl-1-methylxanthine(IBMX)을 배양액에 첨가하여 이들이 난구세포의 분산과 난자의 성숙(핵붕괴)에 미치는 효과를 관찰한 결과 다음과 같았다. 1. Forskilin은 0.001$\mu$M에서 부터 난구세포의 분산을 유도하기 시작하여 (36%) 0.1-10$\mu$M 구간에서 최대의 분산율을 나타내었고 (80-90%) 100$\mu$M에서는 그 효과가 줄어들었다(60%). 이때 난자의 핵 붕괴는 10$\mu$M까지 정상으로 일어나다 (75-80%) 100$\mu$M에서 부분적으로 억제되기 시작하였다(40%). 2. IMBX는 0.01$\mu$M에서 부터 난구세포의 분산을 유도하기 시작하여 (30%) 1-1,000$\mu$M의 전 구간에서 최대의 분산율(81-89%)을 나타내었다. 난자들은 10$\mu$M의 농도까지 정상적으로 핵 붕괴를 일으키었으나(90% 이상) 100$\mu$M 이상에서 급격히 억제되었다(14%). 3. 난구세포의 분산을 유도하는데 필요한 최소의 자극기간을 조사해 본 결과 HCG는 2분, FSH와 forskilin은 15-30분, IBMX는 2시간이었다. 위 결과로 부터 생쥐 난구세포에서 cAMP의 농도를 높이는데 adenylate cyclase 와 phosphodiesterase의 두 효소가 모두 중요한 기여를 하며 난구세포는 단 기간에 생긴 cAMP의 peak로서 분산이 유도될 수 있으나 난자의 성숙억제 과정에서는 지속적인 cAMP의 존재가 필요하다는 것을 알았다.

• 음성과학
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• 제11권1호
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• pp.105-116
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• 2004

The fundamental frequency of a vowel is known to be affected by the identity of the preceding consonant. The general agreement is that strong consonants trigger higher F0 than weak consonants. However, there has been a disagreement on the shape of this segmentally affected F0 contours. Some studies report that shapes of contours are differentiated based on the consonant type, but others regard this observation as misleading. This research attempts to resolve this controversy by investigating shapes and slopes of F0 contours of Korean word level speech data produced by four male speakers. Instead of entirely relying on traditional human intuition and judgment, I employed an automatic F0 contour analysis technique known as tilt parameterisation (Taylor 2000). After necessary manipulation of an F0 contour of each data token, various parameters are collapsed into a single tilt value which directly indicates the shape of the contour. The result, in terms of statistical inference, shows that it is not viable to conclude that the type of consonant is significantly related to the shape of F0 contour. A supplementary measurement is also made to see if the slope of each contour bears meaningful information. Unlike shapes themselves, slopes are suspected to be practically more practical for consonantal differentiation, although confirmation is required through further refined experiments.

• 통합자연과학논문집
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• 제4권4호
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• pp.332-338
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• 2011

This study was conducted to find associations of stress with obesity in female college students in Gwangju. 343 subjects were divided into two groups based on BMI(body mass index). BMI of normal group was $19.3{\pm}1.5$ while that of obese group was $26.4{\pm}3.6$. The measured stress level in obese subjects was higher and they were less capable of controlling obese oriented attitude compared to normal subject. Twenty four hour dietary recall revealed that nutrient intakes of obese subjects were higher in energy, lipid, P and cholesterol. However, they were more deficient in Ca, Fe and vitamin A, compared to those of normal ones. This study suggested severe stress might trigger undesirable dietary behavior leading to increase in food consumption which contribute to obesity. Effective nutrition education program targeting obese female students should be developed and implemented to relieve stress and practice desirable dietary behavior and eating pattern.

• 대한지리학회지
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• 제44권1호
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• pp.77-88
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• 2009

이 연구는 한국에서 풍수와 불교의 교섭이 언제 시작되었고 정치주도세력에 의해 어떻게 전개되었으며 그 결과 사회 및 풍수와 불교 상호간에 어떠한 영향을 미쳤는지를 탐구한 글이다. 역사상에서 풍수와 불교는 융합적인 문화형태로 교섭되어 사찰의 입지 및 기능, 사회 공간적 이데올로기의 형성, 풍수의 도입 및 확산, 비보사탑설의 전개 등에서 영향을 주었다. 풍수와 불교는 신라 하대에 선종의 전래를 계기로 본격적인 교섭을 시작하였고, 나말려초에는 사회변혁을 이끄는 공간적 이데올로기로 기능하였으며, 고려시대를 걸쳐서는 왕권의 중앙집중 및 지방의 효율적인 통어 체계를 구축하기 위한 이념적 장치로 적극 활용되었다. 조선시대에 와서 정치지배권에서의 불교와 풍수의 교섭은 쇠퇴되고 민간부문에서만 미약하게 유지되었다.

• 한국전문물리치료학회지
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• 제24권2호
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• pp.37-47
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• 2017

Background: Active trigger points (TrPs) of the suboccipital muscles greatly contribute to the occurrence of chronic tension-type headache, with increased sensitivity of TrPs and facilitated referred pain. Objects: This study aimed to investigate whether the integration of high-frequency diathermy into suboccipital release is more beneficial than the use of suboccipital release alone. Methods: Thirty subjects were assigned to either experimental group-1 (EG-1) to undergo suboccipital release combined with high-frequency diathermy (frequency: 0.3 MHz, and electrode type: resistive electronic transfer), or EG-2 to undergo suboccipital release alone, or the control group (CG) with no intervention, with 10 subjects in each group. The assessment tools included the headache impact test 6 (HIT-6), perceived level of tenderness, neck disability index, and neck mobility. Intervention was performed for 10 minutes, twice per week, for 4 weeks, and measurements were performed before and after the interventions. Results: The between-group comparison of the post-test values and changes between pretest and post-test showed significant differences for all parameters at p<.05, except for the left-to-right lateral bending range. In the post hoc test, EG-1 showed significant differences for the parameters in comparison with the CG, while no significant differences in the perceived tenderness level, on both temporal regions, were found between EG-2 and CG. Furthermore, the HIT-6 score and perceived tenderness level, in the right temporal region, showed significant differences between EG-1 and EG-2. In the within-group comparison, EG-1 and EG-2 appeared to be significantly different between pretest and post-test (p<.05), except for the perceived tenderness level in the right temporal region, with significance for the EG-1 group only (p<.05). Conclusion: These findings suggest that the suboccipital release technique may be advantageous to improve headache, tenderness, and neck function and mobility, with more favorable effects with the incorporation of high-frequency diathermy.

• Journal of Korea Trade
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• 제27권5호
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• pp.1-22
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• 2023

Purpose - Southeast Asia has been the focus of Korea's foreign investment. Korea has been helping developing countries in Southeast Asia achieve economic growth and win-win cooperation through capital exports. FDI is an important channel for technology diffusion. However, the impact of FDI on the bias of technological progress in the host country is dependent on the host country's own endowment structure and capital-labor factor substitution elasticity. Therefore, the central issue of this paper is to accurately evaluate the impact of Korea's FDI to the four Southeast Asian countries in various industries on their bias of technological progress. Design/methodology - The paper uses macroeconomic data for Korea and four East Asian countries to estimate capital-labor factor elasticities of substitution using nonlinear, seemingly uncorrelated regressions (NLSUR). Then, the biased technological change index (BTCI) is calculated for each country. Finally, panel data analysis is used to explore the impact of Korean FDI in various industries in the four Southeast Asian countries on their own directed technological progress, and a robustness test is conducted. Findings - There is a substitution relationship between capital and labor factors based on their elasticity in Korea, Singapore and the Philippines. There is a complementary relationship between capital and labor factors in Indonesia and Malaysia. According to the BTCI, there is a trend toward labor-biased technological progress in all countries. Korean investments in manufacturing, wholesale and retail trade in the host country trigger capital-biased technological change in the host country; investments in the finance, insurance and information and communication sectors trigger labor-biased technological change. In addition, this paper also confirms that directed technological progress can enable cross-country transmission. Originality/value - The innovation of this paper lies in three aspects. First, we estimate the BTCI for five countries and explore the trend and situation of directed technological progress in each country from each country's own perspective. Second, we explore the impact of Korean FDI in the host country on the bias to its technological progress at the industry level. Second, we explore the impact of Korean FDI in various industries in the four Southeast Asian countries on the four countries' own directed technological progress from a national perspective. Finally, we propose corresponding countermeasures for technological progress from the perspective of inverse factor endowment. These innovative points not only expand the understanding of technological progress and cross-country technology transfer in East Asia but also provide practical references for policy-makers and business operators.