• Development and Reproduction
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• v.21 no.2
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• pp.193-204
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• 2017

The influence of triploidization on histological characteristics of retina, trunk kidney, liver and midgut tissue, and cell cycle of tail fin and gill tissue in far eastern catfish, Silurus asotus were analyzed. In the infertile triploid fish, the nucleus and/or cell size of secondary proximal tubule cells of trunk kidney, hepatocyte and midgut epithelium are much larger than those of the corresponding cells in the diploid fish (P<0.05). However, triploid tissue showed fewer number of outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than those for diploid tissue. The mean percentages of the $G_l-$, the S- and the $G_2+M-phase$ fractions were 92.5%, 3.2% and 4.3% in tail fin tissue of diploid, and 93.4%, 2.6% and 4.0% in those of triploid, respectively. There were no significant differences in the percentages of each cell cycle fraction between diploid and triploid. The mean percentages of each phase fractions were 75.1%, 11.1% and 13.8% in gill tissue of diploid and 85.2%, 8.9% and 5.9% in those of triploid, respectively. The differences of cell cycle between tail fin tissue and gill tissue were statistically significant in diploid and triploid (P<0.05). Also, the differences between diploid and triploid were statistically significant in tail fin tissue and gill tissue (P<0.05). Cyclin D1 and cyclin E expressions were not significantly difference between gill tissue and tail fin tissue, and protein expressions of induced triploid were higher than those of diploid. Results from this study suggest that some characteristics in the triploid exhibiting larger cell and nucleus size with fewer number of cell than diploid can be used as an indicator in the identification of triploidization and ploidy level in far eastern catfish.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.290-290
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• 2017

Rice (Oryza sativa L.) is one of the most consumed staple food crop which is energy source as carbohydrate and also is considered as the important antioxidant sources including various phenolic compounds. According to the increasing demand of healthy life, the concern to antioxidant also is increasing because of its health-promoting effect. Phenolic compounds are one of the plant secondary metabolites class, which shows various benefits to preventing or treating chronic diseases. In this study, we have measured the total phenol content from total 647 rice samples using the Floin-Ciocalteau method, and then were selected 30 rice genetic resources classified with high, middle, and low group on the basis of total phenol content. The average of the total phenol content of each group was high-group ($6892.9{\pm}488.5{\mu}g\;GAE/g$) > middle-group ($1428.1{\pm}76.0{\mu}g\;GAE/g$) > low-group ($97.6{\pm}11.4{\mu}g\;GAE/g$). The selected rice samples were analyzed with LC-MS/MS to find the composition and concentration of individual phenolic in rice grain. High-group and middle-group contained large amounts of protocatechuic acid and (+)-catechin whereas low-group showed limited amount. Among high-group samples, rice samples with black pericarp color (IT 174089, IT 220079, and IT 259958) had high content of peonidin-3-O-glucoside. Further, these black rice samples were special since polydatin, rarely found stilbenoid in rice grain, was detected. Overall, both the sum of phenolic acid and the sum of flavonoid were high-group > middle-group > low-group. Also, each group exhibited different phenolic compositions; high-group consisted of flavonoid more than phenolic acid, middle-group and low-group was comprised of phenolic acid rather than flavonoid, and non-pigmented rice was composed by fully phenolic acid. The total phenol content had positive relationships with the sum of phenolic compound (r = 0.64), the sum of flavonoid (r = 0.74) at the significance level of p < 0.0001. In addition, protocatechuic acid and quercetin showed positive correlation with above phenolic composition parameters; in order, r = 0.98, 0.65 for protocatechuic acid and r = 0.73, 0.78 for quercetin (p < 0.0001). In conclusion, the total phenol content assay showed the possibility of utilization as a phenolic composition indicator in rice grain. Also, this result was suggested study pigment on other material.

• Journal of Dairy Science and Biotechnology
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• v.27 no.1
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• pp.1-12
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• 2009

In 2008, baby formula containing melamine was found to be responsible for a large outbreak of renal failure in infants in China. A total of 294,000 infants were hospitalized, and at least 6 babies died due to ingestion of the tainted formula. Melamine contains high levels of nitrogen (>60%), which is used as an indicator of protein content. Therefore, high levels of melamine in infant formula were thought to be the result of deliberate contamination m an attempt to increase its apparent protein content. Following inspections by China's national inspection agency, assorted products from at least 22 dairy manufacturers across China were found to have varied levels of melamine (range: 0.096196.61 mg/kg). Melamine co-exposure with cyanuric acid can induce acute melamine-cyanurate crystal nephropathy, which can lead to renal failure at much lower doses than if either compound were ingested alone. However, currently, there are very few data on melamine analogues other than cyanuric acid. At an expert meeting of the WHO and FAO held to review toxicological aspects of melamine and cyanuric acid on December 14, 2008, a new tolerable daily intake (TDI) of melamine was established that could be applied to the entire population, including infants. Therefore, a risk assessment of the various theoretical melamine contamination levels in infant formula and selected representative foods (other than infant formula and sole-source nutrition products) is urgently needed for Korean babies and children up to 7 years of age. Although the undetectable level regulation for infant formula may be low enough to guarantee the safety of babies under the age of 1 year (including premature babies), the melamine standard of 2.5 ppm for foods other than baby formula could be insufficient to protect the 95th percentile population aged 1~2 years because of this demographic's high consumption of milk, yogurt, and soy milk (hazard index = 1.79). Because TDIs are chronic values intended to protect an individual over his/her lifetime, occasional modest ingestion in excess of the TDI is not likely to be a health concern. However, children aged 1~2 years may have renal systems that are comparatively more sensitive to the crystallization of melamine and its analogues. Therefore, governmental jurisdictions may need to practice more prudent management of food items that could raise the melamine exposure for this population.

• Korean Journal of Veterinary Research
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• v.42 no.2
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• pp.277-282
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• 2002

This study was conducted to investigate the influences of the rope and the tipping chute restraints on body temperature (BT) and heart rate (HR) as acute response for stress caused by restraining for diagnasis and treatment in cattle. Both parameters were recorded by active biotelemetry. In addition cortisol concentration in blood was analyzed as a indicator for stress response. Twelve cattle were divided into two groups based on hydraulic power, the rope restraint group and the tipping chute restraint group. BT and HR were measured at -30 (base), 0, 10, 20, 30, 40, 50, 60, 90, 120, and 180 minutes, including restraint period from 0 to 30 minutes during the experiment. The results obtained in this study was summarized as follows: 1. BT of the rope restraint group was increased ($39.8{\pm}0.3^{\circ}C$) until 20 min after restraint stress for 30 min, and then maintained with high values to the end of experiment. In the tipping chute restraint group, the BT was increased ($39.6{\pm}0.3^{\circ}C$) until the end of the restraint period, but then showed decrese until the end of experiment. 2. HR of both groups was maximized at the beginning of the restraint stress (P<0.05), and then it was decreased gradually but in the tipping chute restraint group showed increase again at the end of the reatraint stress (P<0.05). 3. The cortisol level of the rope restraint group was increased significantly ($9.72{\pm}5.09{\mu}g/d{\ell}$) until 30 min after the end of the restraint stress (P<0.05) and then decreased, but in the tipping chute restraint group showed great increase ($4.68{\pm}1.56{\mu}g/d{\ell}$) at the end of the restraint stress (P<0.05) and then decreased while the tipping chute restraint group was significantly lower than the rope restraint group 30 min after the restraint stress (P<0.05). In conclusion, this study suggests that the tipping chute restraint produces less response to physical stress than the rope restraint but the time for diagnasis and treatment should be shortened when using the tipping chute restraint.

• Journal of Ecology and Environment
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• v.41 no.7
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• pp.182-190
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• 2017

Background: Baekdudaegan was designated in 2005 as a protected area to prevent destruction and conserve. However, there are many disturbed and destroyed areas. The total disturbed area amounts to $25.9km^2$ (0.94%), including $13.4km^2$ (0.49%) in the core area and $12.5km^2$ (0.45%) in the buffer area. This study aims to classify the vegetation types established in the disturbed areas and diagnose the current conditions for ecological restoration in the forest ecosystem. Methods: We surveyed the vegetation in the disturbed areas of Daegwallyeong and Chupungryeong and the surrounding natural areas. The survey conducted from July to September 2015 targeted a total of 54 quadrats by Braun-Blanquet method (Daegwallyeong, 22; Chupungryeong, 32). We also investigated the height and coverage of each layer. We classified the vegetation types based on the field data and analyzed the ratio of life form and the exotic plants, species richness, and vegetation index (Hcl). The Normalized Difference Vegetation Index (NDVI) was calculated from rapideye satellite imagery in 2014 and 2015. Results: Vegetation types were classified into 11 groups according to the criteria that included successional sere or plantation at first, followed by developmental stage and origins. As a result of the analysis of the survey data, species richness, vegetation index (Hcl), ratio of tree plants, and the NDVI tended to increase, while the ratio of the exotic plants tended to decrease with the time since disturbance. These indicators had the classified values according to the vegetation types with time since the disturbance. Conclusions: These indicators can be effectively used to diagnose the conditions of the present vegetation in the disturbed area of the Baekdudaegan area. In addition, the NDVI might be effective for the diagnosis of the disturbed status instead of the human efforts based on the higher spatial resolution of satellite imagery. Appropriate diagnosis of the disturbed forests in the Baekdudaegan area considering the established vegetation types is essential for the elaboration of restoration plans. In addition, restoration target and level should be different according to the disturbed status of restoration site.

• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.266-273
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• 2008

The effects of soil acidification on the seedling growth and nutrition of Pinus densiflora and Quercus acutissima were investigated. The relationship between the seedling growth and molar (Ca+Mg+K)/Al ratio of in soil solution was examined. The results suggested that growth inhibition of seedling Pinus densiflora and Quercus acutissima was due to the low pH of soil solution, which was followed by leach of Al into soil solution, and decrease of essential elements, such as Ca in aerial pant of the seeding caused by the increase of Al concentration in subterranean pant of the seedlings. The level of growth inhibition was determined not only by Al concentration, but also by the balance of inorganic elements, Al, Ca, Mg and K. The growths of two species in total dry weight were clearly inhibited when molar (Ca+Mg+K)/Al ratio of the soil was lower than 6.0. The growth in dry weight, in the condition of the molar ratio was 0.8, was decreased 60% or 50% for the seedling of Pinus densiflora or Quercus acutissima respectively. It was concluded that the molar (Ca+Mg+K)/ Al ratio could be an important index for evaluation of the effects of soil acidification, due to acid deposition such as acid rain, on growth of trees and nutrition. And it might be a more useful indicator for evaluation of critical load of acid deposition on forest ecosystems.

• Korean Journal of Acupuncture
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• v.21 no.2
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• pp.47-67
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• 2004

Objectives : In the present study, the effect of Scolopendrid Water-Alcohol Extract (SWAE) applied to acupuncture point BL23 (Shinsu) on the neuropathic pain was examined. A common source of persistent pain in humans is the neuropathic pain. Anti-convulsant drugs are used to treat the neuropathic pain. In the oriental medicine, Scolopendrid was used for long time to treat convulsant syndrome and back pain, etc. Methods : On the bases of the Scolopendrid clinical application, the effect of SWAE applied to the acupuncture point was tested in the rat model of neuropathic pain. Neuropathic pain was induced by tight ligation of L5 spinal nerve. When rats developed pain behaviors, One hundred microliter of SWAE was applied into the ipsilateral BL23 point at a dose of 10 mg/ml under enflurane anesthesia. The foot withdraw latency of the hind limb was measured for an indicator of pain level after each manipulation. Results : SWAE injection increased the mechanical threshold of the foot in the rat model of neuropathic pain significantly for the duration of 4h, suggesting a partial alleviation of pain. SWAE applied to BL23 point produced a significant improvement of mechanical sensitivity of the foot lasting for at least 4h. However, neither contralateral BL23 point, ST25 (Chonchu) point, nor LR3 (Taechung) point produce as much increase of mechanical sensitivity as ipsilateral BL23 point. And, this increase of mechanical sensitivity was dose-dependent. The improvement of mechanical threshold was interpreted as an analgesic effect. In addition, the analgesic effect of Scolopendrid 4 mg/kg injection is equivalent to that of gabapentin 50 mg/kg injection. The relations between SWAE-induced analgesia and endogenous nitric oxide(NO), inducible NO synthase (iNOS)/neuronal NO synthase (nNOS) were also examined. Results were turned out that both NO production and nNOS/iNOS protein expression which are increased by nerve injury were suppressed by SWAE injection applied to BL23 point. Conclusions : The data suggest 1) that SWAE produces a potent analgesic effect on the neuropathic pain model in the rat and 2) that SWAE-induced analgesia modulate endogenous NO through the suppression of nNOS/iNOS protein expression.

• Journal of Korean Society of Transportation
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• v.34 no.2
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• pp.107-122
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• 2016

This study aims to develop a framework to estimate mobile source emissions with the macroscopic travel demand model including enhanced estimates of intra-zonal travel emissions using Space Syntax analysis. It is acknowledged that "the land-use and transportation interaction model explains the influence of urban structure on accessibility and mobility pattern". Based upon this theory, the estimation model of intra-zonal travel emissions is presented with the models of total travel distance, total travel demand, and average travel speed of intra-zonal trips. Thess statistical models include several spatial indices derived from the Space Syntax analysis. It explains that urban spatial structure is a critical factor for intra-zonal travel emissions, which is lower in compact zone with smaller portion of land area, lower sprawl indicator, and more grid-type of road network. Also the suggested framework is applied in the evaluation of the effectiveness of bicycle lane project in Suwon, Korea. The estimated emissions including intra-zonal travel is as double as the results only with inter-zonal demands, which shows better performance of the suggested framework for more realistic outcomes. This framework is applicable to the estimation of mobile source emissions in nation-wide and the assessment of transportation-environment policies in regional level.

• Journal of Technology Innovation
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• v.22 no.2
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• pp.1-30
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• 2014

Offset trade has been applied in Republic of Korea's defense acquisition programs since early 1980s and used for national economic development as well as enhancement of military capabilities. The accumulated offset trade value amounts to over 10 billion dollars by 2010. However, questions in regards to the effectiveness of offset trade have been constantly raised. As it is a quite difficult task to analyze and calculate the application performance of defense technologies acquired by offsets objectively, limited level of quantitative analysis of application performance have been conducted so far. Hence, in this paper, we came to understand the overall status of application of those technologies through in-depth performance analysis and suggested some specific policies for the further development of offset trade based on the analysis results. To begin with, we developed a questionnaire based on performance indicator deducted through literature review of relevant researches, and conducted survey of major offset recipients. Also, another survey of offset experts belonged to the army, government organization, research institute and companies was conducted to evaluate the performance and effectiveness of offsets qualitatively. And we analyzed the efficiency of application performance using DEA. The results of all surveys are showing that there is positive accomplishment in the technological aspect, but in economic aspect, it shows relatively inactive outcome. Based on these results, policy direction is considered to be changed from the emphasis on the acquisition of core technology to revitalization of domestic defense industry in line with new government's policy orientation.

• Journal of Korea Water Resources Association
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• v.46 no.7
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• pp.745-754
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• 2013

The systematic analysis and evaluation of required energy in the processes of drinking water production and supply have attracted considerable interest considering the need to overcome electricity shortage and control greenhouse gas emissions. On the basis of a review of existing research results, a practical method is developed in this study for evaluating energy in water supply networks. The proposed method can be applied to real water supply systems. A model based on the proposed method is developed by combining the hydraulic analysis results that are obtained using the EPANET2 software with a mathematical energy model on the MATLAB platform. It is suggested that performance indicators can evaluate the inherent efficiency of water supply facilities as well as their operational efficiency depending on the pipeline layout, pipe condition, and leakage level. The developed model is validated by applying it to virtual and real water supply systems. It is expected that the management of electric power demand on the peak time of water supply and the planning of an energy-efficient water supply system can be effectively achieved by the optimal management of energy by the proposed method in this study.