• Title/Summary/Keyword: Leucocytes

Search Result 122, Processing Time 0.032 seconds

Olive flounder (Paralichthys olivaceus) leukocytes stimulated with poly (I:C) could kill Miamiensis avidus (Ciliophora: Scuticociliatia) only when ciliates were immobilized by antiserum

  • Kang, Yue Jai;Kim, Ki Hong
    • Journal of fish pathology
    • /
    • v.31 no.2
    • /
    • pp.109-113
    • /
    • 2018
  • The purpose of the present study was to determine whether the scuticocidal activity of olive flounder (Paralichthys olivaceus) head-kidney leucocytes can be enhanced by stimulation with polyinosine-polycytosine [poly (I:C)]. The growth of Miamiensis avidus was not affected by exposure to unstimulated or poly (I:C)-stimulated leucocytes alone, heat-inactivated immune serum alone, or unstimulated leucocytes plus heat-inactivated immune serum. However, leucocytes stimulated with poly (I:C) showed clearly high scuticocidal activity against M. avidus in the presence of heat-inactivated immune serum. Furthermore, numerous poly (I:C)-stimulated leucocytes occupied the surface of scuticociliates in the presence of the heat-inactivated immune serum, which led to lysis of scuticociliates. These results suggest that both of the stimulation of leukocytes and the immobilization of scuticociliates are necessary to kill scuticociliates by leukocytes.

Functional studies of granulocytes in ruminants 1. Rapid separation of polymorphonuclear leucocytes from circulating blood in bovine (반추동물에서 과립구의 기능에 대한 연구 1. 소의 순환혈액에서 다형핵백혈구의 신속한 분리)

  • 박일규;윤창용;이정원;송희종
    • Korean Journal of Veterinary Service
    • /
    • v.22 no.4
    • /
    • pp.377-383
    • /
    • 1999
  • Polymorphonuclear (PMN) leucocytes are fundamental importance to the body's defense mechanism and play a major role in the local and systemic reactions to infectious disease. Investigation of the physiological and pathological role of the various leucocyte subtypes in host defence mechanisms is dependent upon the isolation of adequate numbers of viable, pure leucocyte fractions. This report describes the separate frequency of PMN leucocytes both from buffy coat layer and from packed RBC layer when bovine peripheral blood was treated with various anti-coagulants such as acid-citrate-dextrose(ACD), ethyldiaminetetraacetic acid(EDTA), sodium citrate and heparin. The separate frequencies of PMN leucocytes from buffy coat layer was 60.4$\pm$9.6%(heparin), 56.8$\pm$11.8%(sodium citrate), 30.6$\pm$14.1%(ACD) and 6.2$\pm$3.7%(EDTA), in order. Those from packed RBC layer monitored with EDTA, ACD, sodium citrate and heparin was 85.0$\pm$4.7%, 84.3$\pm$5.5%, 83.8$\pm$6.5% and 76.3$\pm$7.7%, respectively. The Ficoll-hypaque(FH) density gradient method was used to remove a small part of lymphocytes and/or monocytes from leucocytes in packed RBC layer. With the result that it increased separate frequency of PMN leucocytes from EDTA(89.9$\pm$2.4%), ACD(89.5$\pm$3.6%), and sodium citrate(83.6$\pm$10.3%) than heparin(68.4$\pm$13.9%). These results indicate that the use of EDTA and ACD as anticoagulant Is suitable for the separation of PMN leucocytes from bovine peripheral blood, and that the FH density gradient method is able to increase the separate frequency of PMN leucocytes from packed RBC layer.

  • PDF

Production of Phagocyte Activating Supernatants by Olive Flounder (Paralichthys olivaceus) Leucocytes Stimulated with Genomic DNA of Escherichia coli

  • Lee Chan Hwei;Kim Dong Soo;Kim Ki Hong
    • Fisheries and Aquatic Sciences
    • /
    • v.5 no.4
    • /
    • pp.258-262
    • /
    • 2002
  • Effects of Escherichia coli genomic DNA on the production of phagocyte activating supernatants by the head kidney leucocytes isolated from olive flounder (Paralichthys olivaceus) were investigated. Phagocyte activating activity of the supernatants was estimated by. measuring reactive oxygen species (ROS) production in target head kidney phagocytes. All supernatants from olive flounder head kidney leucocytes-stimulated with E. coli DNA induced significantly (P<0.01) higher ROS production from target pagocytes than the unstimulated control supernatant. Maximum enhancement of chemiluminescent response was observed $5.0-10.0{\mu}g\;mL^{-1}$ of bacterial DNA while the increment ability was decreased significantly (P<0.01) at the concentration of $20.0{\mu}mL^{-1}$. The results demonstrate that olive flounder head-kidney leucocytes stimulated with bacterial DNA release a soluble phagocyte activating cytokines capable of enhancing the respiratory burst activity from target phagocytes.

Studies on the hematological and blood chemical findings for dry and lactating periods (건유(乾乳)와 필유(泌乳)에 따른 유우(乳牛)의 혈액(血液) 및 혈액화학상(血液化學像)의 변화에 관한 연구)

  • Kang, Chung-boo;Lee, Kuk-cheon;Lee, Soon-sun;Cho, Hee-taek;Ahn, Dong-won
    • Korean Journal of Veterinary Research
    • /
    • v.29 no.4
    • /
    • pp.437-443
    • /
    • 1989
  • This experiment was carried out to investigate the hematological and blood chemical values in dairy cattle during the dry and lactating period. Blood was collected from six healthy dairy cattle in Gyeongnam province. Leucocytes count, hemoglobin concentrations and the percentage of packed cell volume were lowest at the early lactation period. Leucocytes count was not affected during dry the to lactating period. The differential count of eosinophilic leucocytes was low at the late lactation period, while the basophilic leucocytes was high at the dry period. Inorganic phosphorus value was below under normal level. Creatinine value was from 1 to 2mg/100ml of serum. Positive reaction to CRP was shown in normal dairy cattle. The values of AST and ALT were higher during the dry than lactating period. r-GTP and total cholesterol tend to be decreased during the lactating period. CPK value was not affected for dry and lactating period.

  • PDF

The Chromosome Studies in the Korean Population ( A Preliminary Note) (한국인의 염색체에 관한 연구(예보))

  • 김영선
    • The Korean Journal of Zoology
    • /
    • v.7 no.1
    • /
    • pp.29-32
    • /
    • 1964
  • A study on chromosome of leucocytes in blood cultures derived from 6 normal Korean was performed . Exact chromosome counts were carried out on 205 cells in male, 211 in female , of which 86.05% revealed a chormosome mordal number of 46. On the basis of relative chromosome lengths and position of centromeres, the Karyotype that the human chromosomes were classified into 7 groups with 22 airs of autosome and one pair of sex chromosome was determined accoridng to the method of denver report. The chromosome number on metaphase was observed in short term cultures of leucocytes from the peripheral blood of 2 patients with chronic granulocytic leukemia and 1 patient with acute granulocitic leukemia . and the chromosome morpholoogy was also investigated in one acute leukemic patient. In all leukemic cases the leucocytes showed the constant value of 46 in the stem -line of chormosome number. But the frequency of cells with 46 chromosomes appeared in the 3 cases was 67.30% in average with a slightly higher range in hypo-andhyper-diploid chromosome numbers than in normal human, The idiogram analysis did not show any abnormality of chromosome in acute leukemic cells.

  • PDF

Studies on classification of Korean catfish (Silurus asotus) peripheral leucocytes (한국산 메기 (Silurus asotus)의 말초혈액 백혈구의 분류)

  • Park, Sung-Woo
    • Journal of fish pathology
    • /
    • v.8 no.1
    • /
    • pp.47-55
    • /
    • 1995
  • Classification of peripheral leucocytes in Korean catfish (Silurus asotus) were performed. The leucocytes were classified on the basis of their morphological, cytochemieal and phagocytic characteristics. Four types of leucocytes were observed in the peripheral blood. Neutrophils were round and 12 - $15{\mu}m$ in diameter with round, oval or 2 - 3 segmented neuclei. Their cytoplam was stained whitish or grey. Lymphocytes were round and 5.5 - $8{\mu}m$ in diameter with large round neuclei which occupied most of the cells. Monocytes were round or irregular cells and 12 - $15{\mu}m$ in diameter with bean - shaped or irreular neuclei. Thrombocytes were oval or spindle - shaped cells with oval or spindle - neucle. The cells with greyish cytoplasm ranged 7.2 to $14.4{\mu}m$ in length and 5.8 to $7.2{\mu}m$ in width. Only neutrophils showed positive reaction in peroxidase and PAS. Neutrophils and monocytes showed active phagocytosis. As the results it is concluded that peripheral leucocytes of Koran catfish could be classified as lymphocytes, neutrophils, monocytes and thrombocytes.

  • PDF

Antitimor Activity of Some Phytobased Polysaccharides and their Effects on the Immune Function

  • Moon, Chang-Kiu;Sim, Kyl-Soon;Lee, Soo-Hwan;Park, Kwang-Sik;Pyo, Yun-Yeo;Ha, Bae-Jin;Lee, Chong-Chul
    • Archives of Pharmacal Research
    • /
    • v.6 no.2
    • /
    • pp.123-131
    • /
    • 1983
  • Polysaccharide fractions were prepared from Ginseng root, Mori Radicis Cortex (M. R. C. ), Phellodendri Cortex (Ph. C. ), Sappan Wood (S. W. ) and Tigli Semen (T. S.). Water extract was also prepared from the mixture of ph. C., S. W. and T. S. Ginseng polysaccharide and water extract of the mixture showed marked antitumor activity against sarcoma 180. Ginseng polysaccharide showed a mild increasing effect on the number of circulating leucocytes and a marked increasing effect on the number leucocytes and a marked increasing effect on the number of plaque forming cells (PEC). Polysaccharides from ginsing root, S. W., Ph. C. + T. S. and water extract of the mixture showed dramatic inducing activities of periotoneal exudate cells (PEC), polymorphonuclear leucocytes (PMN) and macrophages. These results suggest the possibility that water extract of the mixture may have the lentinan like effect and ginseng polysaccharide may have stimulating effects on the general immune system.

  • PDF

Effect of Quaternary Ammonium Compounds(QACs) on the Immune Response of eel(Anguilla japonica) In vitro (In vitro에서 Quaternary Ammonium Compounds(QACs)이 뱀장어의 면역반응에 미치는 영향)

  • Choi, Min-Soon
    • Journal of fish pathology
    • /
    • v.13 no.2
    • /
    • pp.103-110
    • /
    • 2000
  • The immunostimulatory effects of quaternary ammonium compounds(QACs) were investigated in leucocytes of eel(Anguilla japonica) in vitro. Proliferation of peripheral blood lymphocytes(PBLs) was no significantly affected by QACs, regardless of mitogen(PHA, ConA and LPS) and the concentration of QACs added. QACs heightened the leucocytes function such as respiratory burst activity, phagocytosis and pinocytosis, resulting in significantly increased the bactericidal activity of macrophages. These results suggested that QAC might modulate the immune responses by activation of leucocytes function but not by increment of immunocompetent cell numbers.

  • PDF