• YAKHAK HOEJI
• /
• v.58 no.5
• /
• pp.294-299
• /
• 2014

In this study, we investigate anti-allergic and anti-inflammatory effects of Leonurus sibiricus seed (LSS) extract in basophilic leukemia RBL-2H3 cells. To identify anti-allergic actions of LSS, the degranulation was evaluated in IgE and DNP-BSA stimulated RBL-2H3 cells. At the concentration of $100{\mu}g/ml$ of methanol (MeOH) extract and Methylene chloride (MC) and Ethyl acetate (EtOAc) fractions, the degranulation was significantly inhibited 16.7%, 16.7% and 27.9% respectively. And then, to assess anti-inflammatory effects of LSS, IL-4 and IL-13 mRNA level were detected in PMA/ionomycin (PI)-induced RBL-2H3 cells and cell proliferation and IL-4 mRNA level in isolated splenocytes from Balb/c mice. LSS MeOH extract and MC and EtOAc fractions significantly decreased the level of IL-4 and IL-13 mRNA in PI-induced RBL-2H3 cells and showed inhibitory effects on cell proliferation and expression of IL-4 mRNA level in mouse splenocytes. Taken together, these results suggest that LSS has potential anti-allergic and anti-inflammatory effects and EtOAc fraction is the most effective in regulating immune responses.

• Korean Journal of Plant Resources
• /
• v.13 no.3
• /
• pp.202-207
• /
• 2000

This study was conducted to investigate the characteristics of seed germination pattern by the prechilling and alternating temperature treatments to 16 species of native plant. The seed length, width and thickness ranged from 1.1 to 8.9 mm, from 0.7 to 7.5 mm and from 0.4 to 1.7 mm, respectively. In addition, the 1,000 grain weight of native plants ranged from 0.1 to 8.8g. Germination rate of Melandryum firmum R. and Saussurea pulchella F. with light condition, Capsella bursa-pastoris L. and Leonurus sibiricus L. with dark conditionin of non-treatment were the highest 69, 81,16 and 36%, respectively. Germination rate of Aster scaber T., Cirsium setidens N., Ligularia fischeri T., Plantago asiatica L., and Saussurea sp. of 5$^{\circ}C$ prechilling for 30 days were the highest 84, 29, 29, 57, 78 and 95% with light condition respectively, 30% but Cirsium nipponicum M. only with dark condition. Germination rate of Aralia elata S., Aster koraiensis N. and Synurus deltoides N. of alternating temperature with 8 hours at 3$0^{\circ}C$ and 16 hours at 15$^{\circ}C$ were the highest 2 and 57% with light condition and 52% with dark condition.

• The Korean Journal of Ecology
• /
• v.22 no.1
• /
• pp.59-63
• /
• 1999

To verify allelopathic effects, seed germination and seedling growth test, chemical analysis and bioassay of selected species were carried out with naturally occurring chemicals of Artemisia capillaris. Seed germination ratio of Calamagrostis arundinacea. Youngia denticulata and Lactuca indica var. laciniata showed decrease in proportion to increase in aqueous extracts concentration of A. capillaris. while that of Cosmos bipinnatus and Leonurus sibiricus did not. However, dry weight growth of selected species treated with the same extracts as the above experiment was inhibited remarkably compared to the germination test. In the test at different concentrations of essential oil from A. capillaris, seedling growth of A. princeps var. orientalis and Plantago asiatica was suppressed according to the concentration of the essential oil, and root growth of the selected species was more inhibitory than that of shoot growth. Thirty-six chemical compounds were identified from A. capillaris plant by gas chromatography. Seven compounds out of 36 were bioassayed, and terpinen-4-ol was the most toxic among the tested substances.

• Korean Journal of Plant Resources
• /
• v.26 no.1
• /
• pp.19-25
• /
• 2013

This study focused on the usefulness of plant extracts for a city organic farm. Insecticidal activity of 8 plant species, plants crude extracts by ethanol and water, against third instar larvae (susceptible strain) of Plutella xylostella was evaluated in this study. Ethanol(25%) extracts of plant materials had better insecticidal activity than water extracts at the room temperature. Insecticidal activity were investigated by leaf disc spray method. Five plant extracts (Capsicum annuum, Chelidoniun majus, Leonurus sibiricus, Coptis japonica, Mentha piperascens) showed over 50% insecticidal activity to P. xylostella at the concentration of $1500mg{\cdot}L^{-1}$. Extract of C. annuum showed the highest insecticidal activity (83.3%) against P. xylostella. Phytotoxicity was not observed on Raphanus sativus and Brassica campestris with spray application. Seed germination and growth of two plants were not affected. Extract of C. japonica revealed 73.3% control efficacy against P. xylostella of R. sativus and showed 70% control efficacy against those of B. campestris, respectively, in plastic house. Insecticidal activity against various insects was observed in C. japonica extract as 87.4% for R. sativus and 74.3% for B. campestris in the field. These results suggested that extracts of C. annuum, C. japonica showed the highest insecticidal activity, and could be used as city farm insecticides for organic farming.