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Anti-allergic and Anti-inflammatory Effect of Leonurus sibiricus Seed Ethyl Acetate Fractions  

Jung, Yujung (Natural Products Research Center, KIST Gangneung Institute)
Jeon, Youngsic (Natural Products Research Center, KIST Gangneung Institute)
Kim, Hyung Ja (Molecular Recognition Research Center, KIST)
Kang, Ki Sung (College of Korean Medicine, Gachon University)
Kim, Yong Kee (College of Pharmacy, Sookmyung Women's University)
Kim, Su-Nam (Natural Products Research Center, KIST Gangneung Institute)
Publication Information
YAKHAK HOEJI / v.58, no.5, 2014 , pp. 294-299 More about this Journal
Abstract
In this study, we investigate anti-allergic and anti-inflammatory effects of Leonurus sibiricus seed (LSS) extract in basophilic leukemia RBL-2H3 cells. To identify anti-allergic actions of LSS, the degranulation was evaluated in IgE and DNP-BSA stimulated RBL-2H3 cells. At the concentration of $100{\mu}g/ml$ of methanol (MeOH) extract and Methylene chloride (MC) and Ethyl acetate (EtOAc) fractions, the degranulation was significantly inhibited 16.7%, 16.7% and 27.9% respectively. And then, to assess anti-inflammatory effects of LSS, IL-4 and IL-13 mRNA level were detected in PMA/ionomycin (PI)-induced RBL-2H3 cells and cell proliferation and IL-4 mRNA level in isolated splenocytes from Balb/c mice. LSS MeOH extract and MC and EtOAc fractions significantly decreased the level of IL-4 and IL-13 mRNA in PI-induced RBL-2H3 cells and showed inhibitory effects on cell proliferation and expression of IL-4 mRNA level in mouse splenocytes. Taken together, these results suggest that LSS has potential anti-allergic and anti-inflammatory effects and EtOAc fraction is the most effective in regulating immune responses.
Keywords
Leonurus sibiricus seed; anti-allergy; anti-inflammation;
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