• The Korean Journal of Mycology
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• v.19 no.3
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• pp.214-219
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• 1991

Adenosine-5'-triphosphatase$(F_1-ATPase)$ in the Lentinus edodes was fractionated by ammonium aulfate 30% saturation and purified by Sephadex G-200 gel filtration in three times. Three kinds of protein fractions of $F_1-ATPase$ were isolated from this mushroom, and fraction I and ll showed its activity for the substrate, adenosine-5'-triphosphate. Its optimum pH and temperature were found to be pH 7.6 and $58^{\circ}C$, and its thermal stability was stabled for 30 min. at $20-30^{\circ}C$. The Km value of this enzyme was 1.81 mM.

• The Korean Journal of Mycology
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• v.2 no.1
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• pp.31-33
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• 1974

The four stocks of Lentinus edodes (Berk.) SING.-1-3, 16-3, 5NG2, 5NG3 were used as mating materials. As a result of full stock mating pattern for four stocks, all of mating types formed clamp connection but degrees of formation of clamp connection and period required for the formation were somewhat different among all of the mating types. In the mating of two New Guinean stocks-5NG2, 5NG3-, the mycelial growth and the formation of clamp connection was comparatively reduced.

• BMB Reports
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• v.33 no.1
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• pp.37-42
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• 2000

Based on the study by Leatham and Stabmann concerned with the rates (v) of amines and phenolic compounds oxidation catalyzed by laccase of basidiomycete Lentinus edodes (Berk.) Sing., as well as on the results of semiempirical quantum chemical computations using the PM3 method, the linear correlations of v and lnv values with first vertical ionization potentials of the substrates molecules and radicals derived from them, spin densities on N and O atoms of the above radicals, and with the radicals reorganization energies have been found.

• The Korean Journal of Mycology
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• v.15 no.4
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• pp.224-230
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• 1987

Effects Of organic compound, photosensitizer and $K^+$ ion influx. On the light-induced ATPase of mitochondria in L. edodes purified by linear sucrose density gradient centrifugation were studied. The mitochondrial ATPase activity was investigated by various wavelength illumination at dark state. The mitochondrial ATPase was activated 139% and 128% by 10m mol dithiothreitol and 0.1m mol quinacrine, respectively. This enzyme also was activated 36% by 0.1m mol phenazine methosulfate as photosensitizer. But, 100 mg oligomycin and 1m mol phlorizin inhibited activity of enzyme to 48% and 45%, respectively. Its optimum wavelength was 690 nm on the effect of $K^+$ ion influx, its optimum pH and temperature were found to be 7.2 and $55^{\circ}C$.

• Journal of the Korean Wood Science and Technology
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• v.16 no.3
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• pp.38-47
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• 1988

표고버섯(Lentinus edodes) 균에 의한 졸참나무(Quercus serrata)골목의 미시형태적 변화를 주사형전자현미경 (SEM)을 통해 관찰하였다. 표고 재배 5년후 골목의 중량 감소율은 50%에 달했다. 구성세포중 목섬유는 표고균사에 의해 쉽게 분해되었으나 도관과 유세포는 저항성을 나타냈다. SEM 관찰 결과 표고균사는 세포내강(內腔)에서부터 세포벽을 서서히 분해 박벽화(薄壁化)시켰으며, 또한 Cell corner가 중간층보다 먼저 공격을 받아 분해 되었으나 중간층은 쉽게 분해되지 않았다. 흥미롭게도 연부후(軟腐朽)의 전형적 형태인 2차막에서의 공동(空洞)역시 백색부후균인 표고균사에 의해 형성 되었다. 이상의 관찰결과 표고균은 백색부후와 연부후균이 갖는 미시형태적 특징을 동시에 보여 주었다. 아울러 중간층 보다 Cell corner가 먼저 선택적으로 분해되며, 도관세포벽의 분해저항성을 세포벽 구성 리그닌의 화학적 특성과 연관시켜 논의했다.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1998.10a
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• pp.63-65
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• 1998

최근, 생표고의 수요가 급증하고 있으며 주년적인 생산과 공급을 원활하게 하기 위해서 시설재배가 필수적으로 요구되고 있다. 1996년도에 있어 표고 골목은 8,433천㎥이었는데, 이 가운데 노지재배가 92%, 시설재배가 8%를 각각 차지하고 있으나 생산량에서는 노지재배가 90% 그리고 시설재배가 10%를 차지하고 있다. 또한 표고의 시설재배를 통하여 생산량은 약 20%, 생산액은 약 50% 각각 증가하여 소득 면에서도 기여하고 있다. (중략)

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1999.11a
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• pp.221-224
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• 1999

표고(Lentinus edodes (Berk.) Sing.)는 송이과 Tricholomataceae에 속하는 식용 버섯으로서, 동북아시아 지역에서 가장 인기 있는 재배 버섯이다. 표고의 수요와 생산량을 매년 꾸준히 증가하고 있고, 1985년 표고 생산량은 880톤, 87년 1,080톤, 89년 1,530톤, 91년 1,761톤, 92년의 표고의 재배면적은 397,340평이고, 생산량은 2,253톤이며 금액으로 375억에 이르고 있다. (중략)

• The Korean Journal of Mycology
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• v.19 no.3
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• pp.220-225
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• 1991

Activities of the $F_1-ATPase$ purified from Lentinus edodes were stimulated by $Fe^{3+},\;Fe^{2+},\;Cd^{2+},\;Mg^{2+},\;K^{+}\;and\;Co^{2+}$ but were inhibited by $Zn^{2+},\;Ca^{2+},\;Cu^{2+}\;and\;Ni{2+}$ ion. The enzyme activities were increased 130, 65, 65, 68, 105% and 23% by the 5mM $Fe^{3+}$, 10 mM$Fe^{2+}$, 1mM $Cd^{2+}$, 5mM $Mg^{2+}$, 5mM $K^{+}$ and 5mM$Co^{2+}$ ion addition, respectively, as compared with those not added. The enzyme activities were decreased 18, 19, 27 and 30% by 10 mM $Zn^{2+}$, 10mM $Ca^{2+}$, 0.5 mM $Cu^{2+}$ and 10 mM $Ni^{2+}$ ion, respectively. Anion effects of 10 mM ${Co_3}^{2-}$, 20 mM,$CN^{-}$ 20 mM$CH_3COO^{-}$ and 20 mM ${NO_3}^{-}$ ion were inhibited to the enzyme activities of 98, 95, 70 and 50%, respectively. As increasing of ${SO_4}^{2-}$ ion concentration, the enzyme activity was stimulated and 20 mM ${SO_4}^{2-}$ ion was shown increased of 21%.

• Journal of Bio-Environment Control
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• v.9 no.2
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• pp.120-126
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• 2000

The quality of oak mushoom(Lentinus edodes(Berk) Sing) is sensitively affected by environmental factors, especially moisture by the rain during the growing period. To protect mushrooms from being wet, plastic-covered facilities with side openings are mostly being used. However, the indoor temperature and humidity f the facility without roof opening become higher due to its poor ventilation, and consequently reduce the productivity and quality as well. In this study, we analyzed the ventilation rates and indoor temperatures of improved facilities as affected by the area of roof opening and shading rate by the model. The indoor temperature decreased by more than 2.5$^{\circ}C$ as the shading rate increased from 50% to 90%, and especially the effect of wind speed on indoor temperature was significantly great under as low as 50% of shading rate. The ventilation rate became higher under wind speed of 1~2m.s-1 regardless of the shading rate. As the wind speed increased from 0m.s-1 to 2m.s-1, the indoor temperature decreased by more than 2.$0^{\circ}C$. Moreover, the indoor temperature became lower with increasing roof opening ratio, but showed no significant differences at more than 50% of roof opening ratio. At lower shading rate, the indoor temperature sensitively decreased with increasing area of roof opening. Additionally, we obtained the higher ventilation performance with the area of roof opening more or less equal to side opening, regardless of the wind speed and shading rate.

• Korean Journal of Plant Resources
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• v.9 no.1
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• pp.1-9
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• 1996

The effects of multipore spawn inoculation of Lentinus edodes on the mycelial growth stage and on the quantitative and qualitative yields of fruit-bodies for 5 years were investigated at natural environmental conditions. The spawn inoculation quantity did not affect the mycelial rooting at a range of 3.0kg-6.0kg per $m^3$ of bed-logs while the increase of spawn quantity by multipore inoculation increased the mycelial growth and the inner erosion of bed-logs, resulting in the increased yields of total fruit-bodies of L. edodes per $m^3$ of bed-logs for 5 years but resulting in the shortened major production period of fruit-bodies to the the first three years. Major production of fruit-bodies occurred during May-June and August-September, which was not affected by the spawn inoculation quantity. No significant differences in dry weight of a fruit-body were observed as increasing the spawn quantity by multipore inoculation; however the heaviest dry weight of a fruit-body was 1.63g at the spawn inoculation of 4.0kg per $m^3$/TEX> of bed-logs.