• The Korean Journal of Ecology
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• v.8 no.3
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• pp.141-146
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• 1985

Colonization ability was studied with Lemna paucicostata and Spirodela polyrhiza. Colonization ability of each species was defind as its ability to; (1) tolerate to desiccation, (2) multiplicate and spread in the new habitat, (3) inhibit the other species competitively. Lemna paucicostata was inferior to Spirodela polyrhiza in ability (1), but superior to Spirodela polyrhiza in abilities (2) and (3). Whenever lemna paucicostata and Spirodela polyrhiza are colonized recently, only these three abilities can not explain which species is better colonist. Even short unsuitable periods would eliminate a species, with time lag occurring before recolonization. This would result in a discontinuous of the species concerned.

• Journal of the Korean Chemical Society
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• v.8 no.4
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• pp.164-168
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• 1964

We isolated histone-type basic proteins from lemna paucicostata for the first time. Basic proteins were extracted directly with dilute mineral acids from homogenized lemna paucicostata. Amino acid compositions of basic protein portions adsorbed on Amberlite CG-50(at pH 6. 0) were resembled to those of calf thymus histones. Especially, lysine content was the greatest of the other amino acids. By chromatographic studies, adsorbed portions of basic protein components on carboxymethyl cellulose column(at pH 4. 2) were shown to be homogeneous to calf thymus histones, however, the area under the individual curve was different, and furthermore, the containing of a non-adsorbed portion in the large extent was markedly different from calf thymus histones. And amino acid compositions of adsorbed portions represented the histone-type basic propertes, but non-adsorbed portions were considered as a different protein compared with the typical histone. When calf thymus histone and protein components separated from lemna paucicostata were heated($60^{\circ}C$) with a solution of $HgSO_4-H_2SO_4$, precipitates were not obtained.

• Journal of Animal Environmental Science
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• v.16 no.2
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• pp.143-152
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• 2010

Conditions for artificial culture of Lemna Paucicostata and its nutritional values were examined in this study. Lemna P. was cultured using artificial wastewater and a bioreactor (total volume $2,630\;cm^3$, working volume $2,240\;cm^3$) was operated at conditions of 6,250 lux and $28^{\circ}C$. Water flow affected the growth of Lemna P.: growth rate was very high (more than $1.1\;d^{-1}$) at a condition of no-water movement, but it was very low (less than $0.15\;d^{-1}$) when water moved slowly. The growth of Lemna P. was higher in $16h\;d^{-1}$ light cycle than in Sand $24h\;d^{-1}$, and it was also severely affected by the initial $NH_4$-N levels of wastewater. The growth rate of Lemna P. was high in lower $NH_4$-N level, indicating that the growth rate is in inverse proportion to $NH_4$-N concentration in wastewater. However, the contents of crude protein (CP) of Lemna P. were proportional to the initial $NH_4$-N concentration. The CP contents of Lemna P. cultured at 2, 10, 50 and 100 $NH_4$-N mg $L^{-1}$ was 18, 24, 37, 43%, respectively, showing the Lemna P. cultured at 50 and $100\;mg\;L^{-1}$ had similar protein contents to linseed (CP 35%), cottonseed (CP 38%) and soybean (CP 45%). Fat, protein, fiber, NDF and ADF contents of Lemna P. harvested at conditions of $16h\;d^{-1}$ light cycle and less than $2\;mg\;L^{-1}$ of $NH_4$-N level was 2.8, 18, 27, 20, 41 and 65.7%, respectively. Since the growth rate of Lemna P. was very high (more than $1.1\;d^{-1}$) at those conditions, it was convinced that mass production of valuable protein and fiber sources are feasible. In particular, since the Lemna P. has unsaturated fatty acids found mainly in animal fat as well as beneficial fatty acids to health such as C18:ln9c, C18:2n6c, C20:5n3 and C22:2, the Lemna P. biomass would be a highly valuable alternative feed source to grains.

• Journal of Plant Biotechnology
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• v.36 no.2
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• pp.157-162
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• 2009

High frequency plant proliferation system via direct frond regeneration of endemic duckweed plants Lemna paucicostata and Spirodela polyrhiza was established. Fronds of L. paucicostata and S. polyrhiza were able to multiply half-strength MS basal medium without plant growth regulators. However, addition of BA at a range of 1 to 3 mg/L was more effective than high concentration of BA treatments for fronds proliferation. Also half-strength MS salts was suitable for the fronds proliferation. Increase of salts concentration had inhibitory effect on fronds proliferation. Also the frequency of callus formation from fronds of L. paucicostata was 3.3%, when they cultured onto 1/2 MS medium supplemented with 1 mg/L of BA. Similarly the frequency of callus formation from S. polyrhiza was very low. After subculture of white globular structures derived from fronds of L. paucicostata, numerous globular somatic embryos and calluses were developed onto the surface of fronds. However these somatic embryos did not fully develop into normal plants when transferred to 1/2 MS basal medium. Therefore direct frond regeneration system was more efficient for mass proliferation of L. paucicostata and S. polyrhiza. The plant regeneration system of L. paucicostata and S. polyrhiza established in this study, might be applied to mass proliferation and genetic transformation for molecular breeding.

• The Korean Journal of Ecology
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• v.20 no.3
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• pp.191-200
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• 1997

The present study was undertaken to classify and describe the weed communities of the fields in Korea by methods of the ZM school of phytosociology. On the basis of the data obtained, the following vegetation units were recognized: A. summer weed community of rice field A-1. Oryzetea sativae: Sagittario-Monochorietum, A-2. Lemnetea minoris: Lemna paucicostata-Spirodela polyrrhiza community B. spring weed community of rice field B-1. Bidentetea tripartitae: Stellario-Ranunculetum cantoniensis C. summer weed community of arable land C-1. Chenopodietea Pinellio ternatae-Euphorbietum pseudochamaesyceslsynonym: Acalypho australis-Digirietum pectiniformis and Cephalonoploso segetti-Geranietum eriostemonii of North Korea in Dostal다 et al.(1990)] and Phyllantho urinariae-Lindernietum crustaceae D. weed community of fallow field D-1. Erigeron sumatrensis-Erigeron anmus community, D-2. Digitaria adscendens-Portulaca oleracea community, D-3. Chenopodium album community, and D-4. Erigeron canadensis-Erigeron annuus community. Generally compared to the Korean Peninsula, the weed communities in the fields of Cheju Island is much plentiful in their species composition.

• The Korean Journal of Ecology
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• v.21 no.3
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• pp.203-215
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• 1998

The weed vegetion of the rice fields in south Korea was researched in terms of syntaxonomy and synecology. Total 186 releves were analyzed by the Zurich-Montpellier school's method. 10 syntaxa were recognized: Stellario-Alopecuretum amurensis ass. nov. hoc loco, Alopecuro-Ranunculetum scelerati Miyawaki et Okuda 1972, Hemistepto-Capsellietum bursa-pastoriae ass. nov. hoc loco, Oryza sativa-Echinochloa crusgalli community, Sagittario-Monochorietum plantaginea Miyawaki 1960, Cyperus iria community, Hyperico-Juncetum decipiens ass. nov. hoc. loco, Spirodela-Lemna paucicostata community, Lemno-Salvinetum natans Miyawaki et J. Tuxen 1960. The Oryzo-Echinochloion oryzoides Bolos et Masclans 1955 and the Alopecurion amurensis Miyawaki et Okuda 1972 are representative of the summer annual plant community and the winter annual plant community. It was emphasized that syntaxonomical and synecological study on the ruderal and segetal weed vegetation in Korea should be accomplished in consideration of bioclimatic condition of summer monsoon climate of Korean Peninsula.

• Journal of Wetlands Research
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• v.8 no.2
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• pp.65-74
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• 2006

A study of the vascular hydrophyte communities and biomass was undertaken in the Bigumdo wetlands, Shinangun, from March to November, 2005. As a result, the vegetation was divided into 14 communities. Among them, emergent hydrophytes consisted of 6 communities, including Miscanthus sacchariflorus community, Phragmites communis community, Typha angustata community, Leersia japonica community, Paspalum disticum var. indutum community, and Persicaria thunbergii community, floating hydrophytes 5 communities including Hydrocharis dubia community, Nelumbo nucifera community, Euryale ferox community, Trapa japonica community and Nymphaea tetragona var. angusta community, free-floating hydrophytes 2 commuinties including Lemna paucicostata community and Spirodela polyrhiza community, and submergent hydrophyte 1 community, including Myriophyllum verticillatum community. Biomass was the highest at emergent hydrophytes and decreased along the life form, in the order like floating hydrophytes, submergent hydrophyte and free-floating hydrophytes. In addition, hydrophytes in the Bigumdo wetland showed the typical vertical zonation pattern like a natural swamp. These results were considered that the wetland of Bigumdo was characterized by the typical structure of aquatic plant ecosystem.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.27-39
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• 2005

a-Ketol linolenic acid [KODA, 9,10-ketol-octadecadienoic acid, or 9-hydroxy-10 -oxo-12(Z), 15(Z)-octadecadienoic acid] was found as a stress-induced factor in Lemna paucicostata. KODA reacts with catecholamines to generate many products that strongly induce flowering in L. paucicostata, although KODA itself was inactive. KODA contains an asymmetric carbon at the 9-position in the molecule; the 9-hydroxyl group is predominantly 9R, with an enantiomeric excess of 40% (70% 9R and 30% 9S). We analyzed two major products of the reaction between KODA and norepinephrine, named FN1 and FN2. FN1 was identified as a tricyclic a-ketol fatty acid, 9(R)-11-{(2'R,8’R,10'S,11'S)-2',8'-dihydroxy-7'-oxo-11'-[(Z)-2-pentenyl]-9'-oxa-4'-azatricyclo[6.3.1.01.5]dodec-5'en-10'-yl}-9-hydroxy-10-oxoundecanoic acid. FN2 was the C-9 epimer of FN1. FN1 was derived from 9R-type KODA and FN2 from 9S-type. FN1 showed strong flower-inducing activity, but FN2 was inactive. Pharbitis nil (violet) is a typical short-day plant; flowering can be induced by exposing a seedling cultivated under continuous light to a single 16-h dark period. We analyzed endogenous KODA levels and showed that they were closely related to flower induction: KODA sharply increased in the later part of a 16-h dark period, on the other hand, it failed to increase in the night-break experiment. In addition to it, KODA increased transiently in immature flower buds in all the plants we examined, including P. nil. No such increase of KODA was seen in foliar buds of P. nil. When KODA was sprayed on seedlings of Pharbitis, flower induction was promoted only by the (R)-form of KODA. We also found that KODA enhances flowering in garden plants such as carnations and impatienses. These phenomena indicate that KODA may be involved in flowering formationg of plants and it is potentially useful for a regulating agent for commercial plant flowering.

• Journal of Wetlands Research
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• v.10 no.1
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• pp.31-37
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• 2008

A study of the vascular hydrophyte communities was undertaken in the wetlands of Imjado, Shinangun, from March to November, 2006. As a result, the vegetation was divided into 15 communities. Among them, emergent hydrophytes consisted of 9 communities, including Phragmites communis community, Typha angustata community, Paspalum disticum var. indutum community, Sparganium erectum community, Oenanthe javanica community, Echinochloa crusgalli var. crusgalli community, Juncus effusus var. decipiens community, Aneilema keisak community and Persicaria thunbergii community, floating hydrophytes 1 community Trapa japonica community, free-floating hydrophytes 2 communities including Lemna paucicostata community and Spirodela polyrhiza community, and submergent hydrophyte 2 community, including Myriophyllum verticillatum community and Potamogeton distinctus community. These results were considered that the wetland of Imjado was characterized by the typical structure of aquatic plant ecosystem.

• The Korean Journal of Ecology
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• v.19 no.5
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• pp.431-451
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• 1996

The present studwas undertaken to classify and describe the riverside vegetation around Hanchon (36°27’-36°51’N, 128°15’-128°37’E), a tributary in the upper stream area of Nak-tong River, South Korea by methods of the ZM school of phytosociology. As a result the vegetation was divided into twenty three associations and communities belonging to ten classes. Reflecting various human impacts in the past, the associations and communities of the Artemisietea principis were most plentiful there. The vegetation units obtained in the present study were as follows: A. forest vegetation A-1 Fagetea crenatae:Zelkova serrata community, Larix leptolepis community; B. Shrub vegetation B-1 Salicetea sachalinensis:Salicetum gracilistylae, Salicetum graciliglandis assoc. nov., B-2 Rosetea sachalinensis:Salicetum gracilistylae, Salicetum graciliglandis assoc. nov., B-2 Rosetea multiflorae:Stephanandra incisa community, Lycium chinense community; C. Grassland vegetation C-1 Lemnetea minoris:Spirodela polyrhiza-Lemna paucicostata community, C-2 Bidentetea tripartiti:Polygonetum thunbergii, Polygonetum sieboldii-hydropiperis, Panico Polygonetum hydropiperis, Pharagmitetea :Oenantho- Phalaridetum arundinaceae, Phragmitetum japonicae, Miscanthetum sacchariflori, Phragmites australis community, C-4 Artemisietea principis:Artemisia princeps community, Impatiens textori community, Lactuco indicae-Humuletum japonicae, Pueraria lobata-Humulus japonicus community, Pennisetum alopecuroides community, C-5 chenopodietea:Echinochloa crus-galli var. orizicola community, Digitaria adsecendens community, Polygonum nodosum community, C-6 Miscanthetea sinensis: Miscanthus sinensis community, C-7 Plantaginetea:Eragrotis multicaulis-Plantago asiatica community. It is inferred that the endemic vegetation units in the riverside vegetation of Korea are very rare, because most of the vegetation units obtained in the present study are common with those of Japan. Among the above vegetation units, the Salicetum graciliglandis was established as a new association. On the other hand, the associations and communities of classes characterizing the riverside vegetation increased with a decrease of human impacts from the downstream to the upstream in Hanchon, while the number of the naturalized plants was the reverse. Also based on the present phytosociological work, the relation between the vegetation units and their environmental conditions and the restoration ecology and nature conservation of the riverside vegetation were discussed here in detail.