• Applied Biological Chemistry
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• 제41권4호
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• pp.246-250
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• 1998

The cytotoxic activity of methanol extracts of 25 leguminous seeds in vitro was evaluated by sulforhodamine B assay, using the five human solid A549 lung, SK-OV-2 ovarian, SK-MEL-2 melanoma, XF-498 CNS and HCT-15 colon tumor cell lines. The responses varied with both cell line arid leguminous seed used. Extracts of Canavalia lineata and Glycine soja revealed potent cytotoxic activity against A549 arid SK-MEL-2 cell lines. Moderate activity was observed in the extracts of Cassia obtusifolia and Glyeine max var. chungtae, and C. lineata and Vigna angulasis against SK-MEL-2 and HCT-15 cell lines, respectively. The other seed extracts were ineffective against model tumor cell lines. Because of their potent cytotoxic activities, the activity of each solvent fraction from C. lineata and G. soja was determined and the potent activity was produced from their chloroform fractions. As a naturally occurring therapeutic agent, leguminous seeds described could be useful for developing new types of anti-tumor agents.

• 농업과학연구
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• 제39권4호
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• pp.483-489
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• 2012

Preference of stink bugs to various leguminous seeds was evaluated by using VTS (Video Tracking System) in laboratory. Major soybean stink bugs such as bean bug, Riptortus pedestris (Fabricius), one-banded stink bug, Piezodorus hybneri (Gmelin), eastern green stink bug, Nezara antennata (Scott), and sole bug, Dolycoris baccarum (L.) were significantly most attracted to Cheongjakong, a soybean variety, baited fish-net trap, followed by soybean varieties Ilpumgeomjeongkong and Taekwangkong, a peanut variety Daekwangdangkong, a kidney bean variety Gangnangkong, and a adzuki bean variety Jungwonpat, respectively in a soybean field. VTS observation in laboratory showed that R. pedestris and D. baccarum had significantly higher frequency of visit on Cheongjakong, followed by Ilpumgeomjeongkong. However, P. hybneri, N. antennata and Halyomorpha halys (Stal) had significantly higher number of visits on Cheongjakong, Seonnogkong and Jinpumkong, followed by Ilpumgeomjeongkong. The sojourned time of stink bugs, however, was significantly longer on Cheongjakong regardless of species of the bugs. Accordingly, Cheongjakong was evaluated as the most preferred soybean seed by fish-net trap and VTS. Thus, VTS is found to be an effective means to evaluate the food preference of stink bugs.

• Mycobiology
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• 제38권4호
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• pp.286-294
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• 2010

Epicoccum purpurascens stain 5615 AUMC was investigated for its biocontrol activity against root rot disease caused by Pythium irregulare. E. purpurascens greenhouse pathogenicity tests using three leguminous plants indicated that the fungus was nonpathogenic under the test conditions. The germination rate of the three species of legume seeds treated with a E. purpurascens homogenate increased significantly compared with the seeds infested with P. irregulare. No root rot symptoms were observed on seeds treated with E. purpurascens, and seedlings appeared more vigorous when compared with the non-treated control. A significant increase in seedling growth parameters (seedling length and fresh and dry weights) was observed in seedlings treated with E. purpurascens compared to pathogen-treated seedlings. Pre-treating the seeds with the bioagent fungus was more efficient for protecting seeds against the root rot disease caused by P. irregulare than waiting for disease dispersal before intervention. To determine whether E. purpurascens produced known anti-fungal compounds, an acetone extract of the fungus was analyzed by gas chromatography mass spectrometry. The extract revealed a high percentage of the cinnamic acid derivative (trimethylsiloxy) cinnamic acid methyl ester. The E. purpurascens isolate grew more rapidly than the P. irregulare pathogen in a dual culture on potato dextrose agar nutrient medium, although the two fungi grew similarly when cultured separately. This result may indicate antagonism via antibiosis or competition.

• Journal of Applied Biological Chemistry
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• 제44권1호
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• pp.1-5
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• 2001

The methanol extracts of 25 leguminous seeds in vitro was evaluated for inhibitory activities against the small intestinal $\alpha$-glucosidase of Sprague Dawley male rats. The responses varied both with leguminous seed types and concentrations used. At the concentration of 0.5 mg/ml, the methanol extracts of Cassia obtusifolia, Glycine max var. yagkong, Glycine max var. hooktae, Glycine max var. geumdu, Glycine max var. mejukong, Glycine soja, Phaseolus multiflorus, Pisum sativum, and Vigna sinensis inhibited over 50% of the enzyme activity. The extracts of G. max var. yagkong and V. sinensis showed relatively strong inhibitory activities against $\alpha$-glucosidase at the concentration of 0.1 mg/ml. The activity of each solvent fraction from G. max var. yagkong and V. sinensis was determined, and potent activities were detected from chloroform and butanol fractions, respectively. $IC_{50}$ values of G. max var. yagkong and V. sinensis were 0.06 and 0.19 mg/ml, respectively. As a naturally occurring therapeutic agents, leguminous seeds examined could be useful for developing new types of antidiabetic agents.

• Preventive Nutrition and Food Science
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• 제6권1호
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• pp.38-42
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• 2001

The methanol extracts of 25 leguminous seeds in vitro were evaluated for inhibitory activities against lens aldose reductase of Sprague Dawley male rats. The responses varied with both leguminous seed and concentration used. At the concentration of 0.1 mg/mL, the methanol extracts from Amphicaraea edgeworthii, Canavalia lineata, Gylcine max var. solitae, Glycine max var. yagkong, Glycine max var. hooktae, Glycine max var. bangkong, Glycine max var. geumdu, Glycine max var. chungtae, Glycine max var. mejukong, Glycine soja, Phaseolus radiatus var. geodu, Vicia tetrasperma, Vigna angulasis, and Vigna sinensis inhibited enzyme activity by greatertha 60%. In following study, at the concentration of 0.01 mg/mL, the extracts of C. lineata and V. tetraspermahad relatively strong inhibitory activity against aldose reductase. Because of their potent inhibitory activities, the activity of each solvent fraction from C. lineata and V. tetrasperma was determined, and the potent activity was showed from chloroform and hexane fractions, respectively. {TEX}$IC_{50}${/TEX} values of C. lineata and V. tetrasperma were 0.004 and 0.006 mg/mL, respectively. As a naturally occurring therapeutic agent, leguminous seeds described could be useful for developing new agents of antidiabetic complications.

• 한국응용곤충학회지
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• 제43권2호
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• pp.123-127
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• 2004

두류품종의 먹이조합에 따른 톱다리개미허리노린재의 약충발육, 성충수명 및 산란에 미치는 효과를 조사하였다. 약충기간은 20-28일로 장유콩(새알콩), 장유콩＋강낭콩 및 장유콩＋땅콩의 조합에서 가장 많았으며, 강낭콩에서 가장 길었다. 영기별 발육기간은 1령에서 2.4-2.5일, 2령에서 4.0-5.1일, 3령에서 4.2-6.0일, 4령에서 4.4-6.9일 그리고 5령에서 5.3-7.3일로 영기가 증가할수록 길어졌으며, 1령을 제외한 타령기에서 발육기간은 먹이조합에 따라 유의한 차이가 있었다. 우화율은 3.5-65%로 먹이조합에 따라 현저한 차이가 있었는데, 장유콩＋땅콩에서 65%로 가장 높았으며, 강낭콩에서 3.5%로 가장 낮았다. 약충사망율은 먹이조합에 관계없이 1령에서 가장 높았다. 성충수명은 5.5-11.2일로 장유콩＋땅콩에서 가장 길었으며, 강낭콩＋땅콩에서 가장 짧았고, 장유콩, 강낭콩, 땅콩 및 장유콩＋강낭콩에서 8.0-8.4일로 먹이에 따른 유의한 차이가 없었다. 총 산란수는 1.0-20.0개로 전체적으로 산란수가 매우 낮았으나, 장유콩＋땅콩＞장유콩＞딴콩＞장유콩＋강낭콩＞강방콩＞강낭콩＋땅콩의 순으로 많았다. 따라서 두류품종의 종자를 이용한 톱다리개미허리노린재의 사육은 장유콩＋땅콩 조합에서 가장 좋았으며, 다음은 장유콩이였다.

• 한국환경복원기술학회지
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• 제19권1호
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• pp.61-71
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• 2016

The purpose of the research is to identify the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria as leguminous woody plants for the cut-slope revegetation. To investigate the coverage ratio and appearance frequency, we divided Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria into five treatment groups with various quantities of $0.0g/m^2$, $1.0g/m^2$, $2.4g/m^2$, $3.8g/m^2$, and $5.2g/m^2$. For each treatment group, we mixed the identical quantities of herbaceous flowers (Lotus corniculatus var. japonicus, Dianthus sinensis, Aster yomena and Pennisetum alopecuroides) seeds and cool-season turfgrasses (Festuca arundinacea and Poa pratensis) seeds. In this result, as the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria increased in the spray, the coverage ratio for leguminous woody plants appeared to increase whereas the coverage ratio decreased in herbaceous flowers and cool-season turfgrasses. However, when the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria exceeded $3.8g/m^2$ in the spray, the coverage ratio of leguminous woody plants rather decreased compared to four treatment groups less than $3.8g/m^2$ seeding ratio. Based on the longitudinal data of coverage ratio in five treatment groups, we observed the gradual process of a short-term succession in which the dominant species shifted in the following order: First, cool-season turfgrasses; Second, herbaceous flowers; Third, leguminous woody plants. Comparing the appearance frequency of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria, Lespedeza cyrtobotrya appeared more frequent in 2014 whereas Indigofera pseudo-tinctoria appeared to be relatively more frequent in 2015. As a result, Indigofera pseudo-tinctoria was discovered to be a dominant species among woody plants. In this study, we observed that the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria was $2.4g/m^2{\sim}3.8g/m^2$. Moreover, the coverage ratio of 29.1%~35.4% and appearance frequency of 4.6plants~5.8plants were found in the condition of optimal seeding quantity.

• 환경생물
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• 제34권4호
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• pp.346-352
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• 2016

팥바구미(Callosobruchus chinensis L.)의 산란 선호성 및 성장 조사를 위해 6가지 콩과식물 종자인 팥, 흑태, 백태, 서리태, 서목태, 강낭콩을 선택, 실험을 하였다. 각 종자당 산란수는 팥 1.23개, 서목태 1.13개, 서리태 1.05개, 백태 0.69개, 흑태 0.61개로 나타났으며, 둘째 날(48 h) 최고 산란수, 넷째날(96 h) 최저 산란을 나타냈다. 유충 성장기간이 기주식물에 따라 2배 이상 증가하는 것으로 나타났다. 우화율은 팥, 서리태, 흑태, 백태, 서목태(83.33%, 28.23%, 27.87%, 20.44%, 11.59%) 순으로 나타났으며, 모든 종자에서 팥과 3배 이상의 차이를 보였다. 성충 수명은 암컷보다 수컷이 대부분 길었고 암컷의 경우 팥 9.43일, 서리태 8.86일, 흑태 7.88일, 백태 7.17일, 서목태 6.80일로 나타났다. 각 성충의 무게는 암수 모두 팥에서 가장 무겁고, 수컷은 서목태, 암컷은 백태에서 최소 무게를 나타냈다. 결과적으로, 팥바구미는 팥 이외의 기주에서는 산란, 우화율, 성충 수명, 성충 무게 감소가 나타났고 반면 우화시기도 지연되었다. 한편 강낭콩에서 산란과 우화는 전혀 없었는데 이것은 강낭콩 종피에 산란과 우화 억제물질이 있음을 시사하며, 이러한 물질의 활용으로 콩바구미과 피해를 조절할 수 있을 것으로 여겨진다.

• 농약과학회지
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• 제2권3호
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• pp.21-27
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• 1998

25종 콩과식물 종자의 메탄올 추출물을 대상으로 기주식물상의 온실실험조건에서 6종의 주요 식물병원균에 대해 방제효과를 조사한 그 효과는 콩과식물의 종류에 따라 커다란 차이를 보였다. 25종의 공시종자를 5 mg/pot 농도로 처리하였을 때 결명자 (C. obtusifolia), 소리테(G. max var. solitae), 약콩(G. max var. yagkong), 흑태(G. max var. hooktae), (P. multiflorusf), (P. radiatus var. aurea)과 (V. sinensis) 추출물들은 오이잿빛곰팡이병, 밀붉은녹병 및 보리횐가루병에 대하여 80% 이상의 방제효과를 나타내었을 뿐 아니라, 잿빛곰팡이병의 살균제 저항성 균주에 대해서도 높은 활성을 보였다. 그러나, 공시종자 모두 벼도열병, 벼잎집무늬마름병 및 Phytophthora infestans병에 대해서는 거의 활성이 없거나 활성이 낮았다. 이상의 결과로부터 잿빛곰팡이병, 밀녹병 및 보리흰가루병 등에 높은 방제효과를 보인 상기 종자추출물들은 식물병 방제제로서 사용 가능성이 예상되었으며, 특히 살균제 저항성 잿곰팡이병에 높은 활성을 보여 시설원예재배에서 문제시되고 있는 잿빛곰팡이병의 방제에도 크게 이용할 수 있을 것으로 기대되었다.

• Journal of Forest and Environmental Science
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• 제28권4호
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• pp.212-219
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• 2012

An experiment was set to understand the seedlings growth and nodulation capabilities of five potentially important leguminous agroforestry tree species of Bangladesh in response to application of organic fertilizer. Study was carried out in nursery bed on the seedlings of Acacia mangium Willd., Acacia hybrid, Acacia auriculiformis A. Cunn. ex Benth, Albizia lebbeck (L.) Benth. and Leucaena leucocephala (Lam.) de Wit. after sowing their seeds in polybags filled with different medium. Farmyard manure or decomposed cow dung was applied as the source of organic fertilizer with other potting media. The growth and nodulation performance of seedlings of the selected species were measured three moths after the first seed was emerged. The different soil: organic manure ratios' used during the experiment were, 1:1, 2:1 and 3:1. The effects of organic fertilizer on growth and nodulation were compared to that of seedlings grown in control. The results revealed a positive correlation between seedling growth and the different doses of organic fertilization (i.e. seedling growth was enhanced significantly with different treatments containing higher proportion of organic fertilizer). In few cases a negative effect was observed after applying higher levels of organic manure. It was also apparent that the nodulation in terms of nodule number and size was inhibited significantly by the application of organic fertilizer.