• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.517-522
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• 2007

The aim of this study was to compare in vitro gas production kinetics, metabolizable energy (ME), organic matter digestibility (OMD) and microbial protein (MP) production of widely used legume hays in ruminant nutrition in Turkey. Gas production were determined at 0, 3, 6, 12, 24, 48, 72 and 96 h and their kinetics were described using the equation p = a+b ($1-e^{-ct}$). There were significant differences among legume hays in terms of chemical composition. The crude protein content of legume hays ranged from 11.7 to 18.6% of dry matter (DM); crude fat from 2.1 to 3.5% DM; neutral detergent fiber from 35.6 to 52.0% DM; acid detergent fiber from 32.0 to 35.5% DM and acid detergent lignin 1.7 to 11.0% DM. Total gas production after 96 h incubation ranged between 61.67 and 76.00 ml/0.200 g of substrate. At 24, 72 and 96 h incubation the total gas production for common vetch were significantly (p<0.01) higher than those of the other legume hays. The ME, OMD and MP of legume hays ranged from 9.09 to 11.12 MJ/kg DM, 61.30 to 75.54% and 90.35 to 138.05 g/kg DM, respectively. The ME, OMD and MP of common vetch was significantly (p<0.01) higher than those of the other hays due to low cell-wall contents and high crude protein. At the end of the experiment, differences in chemical composition of legume hays resulted in the differences in the in vitro gas production, gas production kinetics and the estimated parameters such as ME, OMD and MP. Common vetch can be recommended to hay producers and ruminant breeders, due to high ME, OMD and MP production.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.351-352
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• 1989

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1278-1285
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• 2008

Measurement of gas produced during in vitro fermentation was used to investigate the fermentation characteristics and interactions of rice straw, wheat straw or maize stover mixed with alfalfa or clover at proportions of 100:0, 75:25, 50:50, 25:75 and 0:100, respectively. Cumulative gas production was recorded at 2, 4, 8, 12, 16, 24 and 48 h of incubation, and the Gompertz function was used to describe the kinetics of gas production. In vitro dry matter and organic matter disappearances (IVDMD and IVOMD) were determined after 48 h incubation. The rate of gas production of clover was higher (p<0.05) than that of rice straw, wheat straw, maize stover and alfalfa when straws and hays were incubated separately. Increasing the proportion of alfalfa in the straw-alfalfa mixtures increased (p<0.05) the rates, but not the maximum volume of gas production. However, both rate and the maximum volume of gas production were increased (p<0.01) as the proportions of clover increased in the straw-clover mixtures. The cumulative gas production at 48 h, IVDMD and IVOMD showed no consistent interaction effects between different mixtures of cereal straws and hays. The extent of interactive effects was affected by the types of cereal straw, legume hay and their proportions in the mixture. The appropriate combination for the mixture of rice straw or maize stover with leguminous hays was 75:25 and 25:75, respectively. The better combination occurred at a proportion of 50:50 for the mixture of wheat straw and alfalfa. We conclude that the suitable proportion of low-quality straw and high quality legume hay combination should be considered in the ration formulation system of ruminants according to the extent of positive interactive effects.

• Asian-Australasian Journal of Animal Sciences
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• 제22권7호
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• pp.1005-1015
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• 2009

Two experiments were carried out to determine the utility of sainfoin hay, a temperate tanniferous forage legume, as a dietary supplement for grass-fed cows. The condensed tannins (CT) of sainfoin might counteract the typical metabolic ammonia load of cows in intensive grazing systems. Furthermore, the physical fibrousness of sainfoin might improve ruminal pH stability. In the preliminary experiment, the eating rate of non-lactating Holstein cows of two tanniferous hays, sainfoin and birdsfoot trefoil, was compared to that of a grass-clover hay after specific periods of time (n = 4). The eating rate of sainfoin was superior to that of the other forages. In the main experiment, designed as a replicated 3${\times}$3 Latin square, six ruminally-cannulated, lactating Red Holstein cows received grass, concentrate and either no supplementation, 3 kg/d of grass hay or 3 kg/d of sainfoin hay (n = 6). Measured intakes of the grass hay and the sainfoin hay were 2.0 and 1.5 kg DM, and two cows entirely refused to eat the sainfoin hay and had to be excluded from data analysis. Grass DMI was similar for cows supplemented with sainfoin hay and cows fed only grass whereas intake of concentrate was higher (p<0.01) for the latter treatment. Continuous measurement of ruminal pH showed that the minimum pH at night tended to be lower (p<0.10) with grass-only feeding compared to sainfoin supplementation, but pH did not decline below the threshold of subacute acidosis for a longer period of time. The slightly higher intake of nitrogen (N) for cows supplemented with sainfoin hay (413 g/d) compared to cows fed only grass (399 g/d) was accompanied by an increased (p<0.05) fecal N excretion and a tendency for an increased (p<0.10) urinary N excretion. Ruminal ammonia concentration, as well as plasma and milk urea, were not affected by sainfoin supplementation. In conclusion, the lack of positive effects typical for CT might be explained either by the limited CT content of this plant species (55 g/kg DM) or the relatively low proportion of sainfoin in the total diet or both. Moreover, due to the unexpected low grass quality, the general ammonia load might have been too low for CT to have an impact.

• 한국초지조사료학회지
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• 제38권1호
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• pp.39-43
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• 2018

The objective of this study was to determine the effect of various roughage sources on nutrient digestibility and enteric methane ($CH_4$), and carbon dioxide ($CO_2$) production in goats. Four castrated black goats ($48.5{\pm}0.6kg$) were individually housed in environmentally controlled respiration-metabolism chambers. The experiment design was a $4{\times}4$ balanced Latin square design with 4 roughage types and 4 periods. Alfalfa, tall fescue, rice straw, and corn silage was used as representative of legume, grass, straw, and silage, respectively. Dry matter digestibility was higher (p < 0.001) in corn silage than in alfalfa hay. Dry matter digestibility of alfalfa hay was higher than those of tall fescue or rice straw (p < 0.001). Neutral detergent fiber digestibility of tall fescue was lower (p < 0.001) than those of alfalfa, rice straw, or corn silage. Daily enteric $CH_4$ production and the daily enteric $CH_4$ production per kilogram of $BW^{0.75}$, dry matter intake (DMI), organic matter intake (OMI), digested DMI, and digested OMI of rice straw did not differ from those of tall fescue but were higher (p < 0.001) than those of alfalfa or corn silage. Roughage type had no effect on enteric $CO_2$ emission in goats. Straw appeared to generate more enteric $CH_4$ production than legume or silage, but similar to grass.

• Journal of Animal Science and Technology
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• 제46권3호
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• pp.451-458
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• 2004

우리나라에서는 봄철에 일년생 콩과목초를 수확한 후 포장건조시 목초의 품질에 관한 연구는 거의 없는 실정이다. 따라서 본 시험은 일년생 콩과목초인 crimson clover(Trifolium incarnatum L.), bolta balansa clover(Trifolium balanansae L.) 및 persian clover(Trifolium resupinatum L.)를 봄에 건조하였을 때 건초의 품질에 관한 정보를 얻기 위하여 수행하였다. 일년생 콩과목초의 수확시 건물률은 crimson slover가 24.7%로 가장 높았고, bolta balansa 및 persian clover는 각각 20.4 및 18.8%였다. 콩과목초의 포장 건조후 수분 함량은 persian clover가 가장 낮았으며, 수분함량 20%도달시간은 공시초종 모두 4일이 소요되었다. 반전횟수는 건초의 수분 함량에 영향을 미치지 않았으며, 포장건조 기간중 변화 양상은 비슷하였다. Persian clover의 건물당 잎과 줄기의 비는 crimson 및 bolta balansa clover보다 높았다. 조단백질 함량도 잎이 많은 persian clover가 19.5%로 다른 초종보다 높았다. 포장 건조중 반전횟수를 증가함에 따라 조단백질 함량은 17.8%에서 16.5%로 감소하였다. Persian clover의 NDF 및 ADF함량은 다른 일년생 콩과목초보다 낮았다. 반전횟수에 따른 비교에서는 3회 반전의 NDF 및 ADF 함량이 1회 및 2회 반전보다 높았다. Persian clover의 상대사료가치(RFV)는 178로 가장 높았으며, 이는 건초품질등급의 최상급에 해당된다. Crimson 및 bolta balansa clover의 RFV도 1등급으로 고품질의 건초였다. 반전횟수에 의한 상대사료가치는 반전횟수를 증가함에 따라 150에서 140으로 감소하였다. 이상의 연구결과를 볼 때 persian clover가 식물체 부위중 잎이 많고, 줄기가 비워있고 다른 초종보다 숙기가 늦어, 일년생 콩과목초 중에서 건초 품질이 우수하였다. 그리고 포장건조시 품질손실이 적은 콩과목초를 생산하기 위해서는 반전횟수를 2회이하로 하여야 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제10권4호
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• pp.378-384
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• 1997

The ruminal solubility of copper (Cu), manganese (Mn), molybdenum (Mo), and zinc (Zn) in eight Philippine forages was studied. These forages were: 1) grasses: paragrass (Brachiaria mutica (Forsk.) Stapf), stargrass (Cynodon plectostachyum Pilger), and napiergrass (Pennisetum purpureum Schumach.); 2) creeping legumes: calopogonium or calopo (Calopogonium muconoides Desv.) and centrocema (Centrocema pubescens Benth.); 3) tree lequmes: gliricidia or kakawate (Gliricidia sepium (Jacq.) Walp.), leucaena or ipil-ipil (Leucaena leucocephala (Lam.) de Wit.), and sesbania or katuray (Sesbania grandiflora (L.) Poir). Nylon bags with samples were incubated for 0, 3, 6, 12, 24, 48 and 72h in three rumen cannulated sheep fed with timothy hay (Phleum pratense L.) at 2% body weight/d. The 0-h bags were washed with deionized water. There were species differences (p < 0.05) on the different solubilities of trace elements. At O-h incubation, the trend of solubility was Mo (54%) > Zn (43%) > Cu (38.7%) > Mn (29.5%). At 3-h incubation, all the elements except Mn had an average solubility above 50%. Combining particulate passage rate (1.9%/h) and various trace elements disappearance rates, the effective ruminal solubilization (ERS) of trace elements were computed. The ERS across species ranged from 44.6 to 89.9% for Cu, 29.9 to 84% for Mn, 66 to 95.1% for Mo, and 30.1 to 82.3% for Zn resulting to a trend of Mo > Zn > Cu > Mn.

• 한국초지조사료학회지
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• 제19권2호
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• pp.127-132
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• 1999

우리나라에서는 봄철에 콩과목초를 수확한 후 포장 건조시 목초의 수량 및 품질손실에 관한 연구는 거의 없는 실정이다. 따라서 본 시험은 콩과목초인 알팔파(Medicago sativa L.), 크림손 클로버 (Trifolium incarnatum L.) 및 레드 클로버(Trifolium pratense L.)를 봄철 포장에 건조하였을 때 콩과목초의 수량 및 품질 손실에 관한 정보를 얻기 위하여 수행하였으며 결과는 다음과 같다. 알팔파의 수확시 건물 함량은 15%로 가장 높았으며, 크림손 및 레드 클로버의 건물 함량은 각각 12.4 및 11.5%였다. 이러한 초종간의 건물 함량의 차이는 포장 건조기간 중에 계속 유지되었다. 건물 함량이 80% 이상이 되는데 알팔파는 7일이 소요되었으나, 크림손 및 레드 클로버는 8일이 소요되었다. 크림손 클로버 및 알팔파의 건물당 잎과 줄기의 비는 레드클로버보다 높았으나, 조단백질 함량을 가지고 비교할 때 잎과 줄기 비는 레드 클로버가 크림손 클로버 및 알팔파보다 높았다. 건조기간중 탈엽에 의한 건물 손실률은 알팔파가 크림손 클로버 및 레드 클로버보다 높았으며, 조단백질의 손실률도 초종간의 차이가 같은 경향을 보였다. 생초와 건초의 사료가치 비교에서 생초는 조단백질(CP), 가소화영양소총량(TDN) 및 상대사료가치(RFV)에서 높았고, 건초는 ADF 및 NDF 함량이 낮았다. 이상의 연구결과를 볼 때 봄철(5월 중순)에 포장에서 건조된 건초는 건조기간 중 탈엽에 의한 조단백질의 손실이 높기 때문에 ADF 및 NDF 함량보다는 조단백질 함량에 더 영향을 미치는 것을 알 수 있다.

• 한국초지조사료학회지
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• 제19권3호
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• pp.189-196
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• 1999

야생 코리언 레스페데자의 조사료원으로서의 활용가치를 구명하고자, 숙기별 건물수량, 화학적 성분과 재래산양 12두를 공시하여, 혼합목초(GS) 100% 급여구, GS 80% + Wild Korean lespedeza(LA) 20% 급여구 및 GS 60% + LA 40% 급여구 등의 3처리를 두어 섭취량, 건물소화율 및 질소와 에너지이용성 등을 조사한 결과는 다음과 같다. 코리언 레스페데자의 평균 건물수량은 7,336kg/ha이었으며, 생육이 진행됨에 따른 건물수량은 현저히 증가되는 양상을 보였다(P<0.05). CP 함량은 생육이 진행됨에 따라 현저히 감소된 반면에 섬유소 물질의 함량은 급격히 증가되는 양상을 보였다(P<0.05). 탄닌 함량은 화아생장기(54.5mg/g)에 가장 높았으나, 생육이 진행됨에 따라 감소되는 양상을 보여 종자성숙기에는 26.8mg/g으로 낮아졌다(P<0.05) 건물섭취량은 GS에 LA의 급여비율이 높아질수록 증가되었으나 처리간에 차이가 없었다. 그러나 건물, 세포내용물 및 NDF 소화율은 GS 100% 급여구에 비하여 LA 20% 및 40% 혼합구에서 모두 높은 결과를 보였다(P<0.05). 가소화질소 축적율은 처리간에 차이가 없었으나, 대사질소 축적율과 생물가는 GS 100% 급여구에 비하여 GS 60% + LA 40% 급여구에서 높았다(P<0.05). 가소화 및 대사에너지의 이용율은 처리간에 차이가 없었던 것으로 보아 에너지이용성에 미치는 코리언 레스페테자의 영향은 적었다. 이상의 결과로 보아 야생 코리언 레스페데자의 건물수량, 건물소화율 및 질소 이용성을 고려할 때, 부존 조사료원으로 이용가치는 충분하다고 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권2호
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• pp.181-186
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• 2017

Objective: Leucaena leucocephala (Leucaena) is a perennial tropical legume that can be directly grazed or harvested and offered to ruminants as hay, silage, or fresh. However, Leucaena contain phenolic compounds, which are considered anti-nutritional factors as these may reduce intake, digestibility and thus animal performance. Therefore, the objective of this experiment was to determine effects of Leucaena silage (LS) feeding levels on rumen microbial populations, N-balance and microbial protein synthesis in dairy steers. Methods: Four, rumen fistulated dairy steers with initial weight of $167{\pm}12kg$ were randomly assigned to receive dietary treatments according to a $4{\times}4$ Latin square design. Treatments were as followings: T1 = untreated rice straw (RS; Control), T2 = 70% RS+30% LS, T3 = 40% RS+60% LS, and T4 = 100% LS. Dairy steers were fed rice straw and LS ad libitum and supplemented with concentrate at 0.2% of body weight/d. Results: Results revealed that the rumen microbial population, especially cellulolytic, proteolytic bacteria and fungal zoospores were enhanced in steers that received 60% of LS (p<0.05), whereas the amylolytic bacteria population was not affected by treatments (p>0.05). Protozoal population was linearly decreased with increasing level of LS (p<0.05). Moreover, N-balance and microbial protein synthesis were enhanced by LS feeding (p<0.05) and were the highest in 60% LS group. Conclusion: Based on this study, it could be concluded that replacement of RS with 60% LS significantly improved microbial population and microbial protein synthesis in diary steers.