• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1165-1176
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• 2019

Botulinum neurotoxins (BoNTs), produced by Clostridium botulinum, are the most toxic substances known. However, the number of currently approved medical countermeasures for these toxins is very limited. Therefore, studies on therapeutic antitoxins are essential to prepare for toxin-related emergencies. Currently, more than 10,000 Halla horses, a crossbreed between the native Jeju and Thoroughbred horses, are being raised in Jeju Island of Korea. They can be used for equine antitoxin experiments and production of hyperimmune serum against BoNT/A1. Instead of the inactivated BoNT/A1 toxoid, Halla horse was immunized with the receptor-binding domain present in the C-terminus of heavy chain of BoNT/A1 (BoNT/A1-HCR) expressed in Escherichia coli. The anti-BoNT/A1-HCR antibody titer increased rapidly by week 4, and this level was maintained for several weeks after boosting immunization. Notably, $20{\mu}l$ of the week-24 BoNT/A1-HCR(-immunized) equine serum showed an in vitro neutralizing activity of over 8 international units (IU) of a reference equine antitoxin. Furthermore, $20{\mu}l$ of equine serum and $100{\mu}g$ of purified equine $F(ab^{\prime})_2$ showed 100% neutralization of 10,000 $LD_{50}$ in vivo. The results of this study shall contribute towards optimizing antitoxin production for BoNT/A1, which is essential for emergency preparedness and response.

• Mycobiology
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• 제33권1호
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• pp.61-64
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• 2005

Three species of the Laboubeniales from Korea are described. They are new to the mycological flora of Korea. Corethromyces purpurascens on several parts of Ochthephilum densipenne, Laboulbenia barbara on the metasternum of Philonthus longicornis and L. compressa on the elytra of Harpalus tinctulus were found.

• 생약학회지
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• 제18권2호
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• pp.103-106
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• 1987

Two diterpene alkaloids were isolated from Aconitum napiforme which is a native plant in Korea, and identified as hypaconitine and mesaconitine by UV, IR, MS and NMR data.

• 한국의학물리학회:학술대회논문집
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• 한국의학물리학회 2006년도 제33회 추계학술대회 발표논문집
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• pp.34-34
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• 2006

• 한국광학회:학술대회논문집
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• 한국광학회 2007년도 동계학술발표회 논문집
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• pp.24-25
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• 2007

• 한국진공학회:학술대회논문집
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• 한국진공학회 2006년도 제30회 학술논문발표회 초록집
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• pp.90-90
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• 2006

• Mycobiology
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• 제34권1호
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• pp.41-43
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• 2006

Two species of the Laboubeniales based on the Tibetan collections are described. They are new to the mycological flora of Tibet. Laboulbenia polyphaga found on several parts of Amara majuscula was characterized by having the outer appendages not ramified and simple. Peyritschiella protea obtained on the lower abdomen of Philonthus wuesthoffi, had two perithecia and two antheridia produced on the third layer of receptacle.

• Bulletin of the Korean Chemical Society
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• 제33권6호
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• pp.2059-2062
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• 2012

• 대한생식의학회:학술대회논문집
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• 대한불임학회 1999년도 제38차 추계 학술대회
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• pp.83.1-83.1
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• 1999

• Bulletin of the Korean Chemical Society
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• 제15권6호
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• pp.424-427
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• 1994

Effects of iron compounds in known biomimetic oxidation systems (Gif IV and GoAgg II) have been studied on activity and ketone/alcohol selectivity of cyclohexane oxidation. Both ketone/alcohol ratio and cyclohexane conversion were affected by counter-ion Z of iron compounds Z-Fe. When Z has a more electron withdrawing property, the reactivity is increased and the formation of ketone is favored. From these experimental results, a new mechanism is proposed for the biomimetic oxidation system.