• Proceedings of the Korean Society of Crop Science Conference
• /
• 2019.04a
• /
• pp.56-56
• /
• 2019

• Water for future
• /
• v.55 no.4
• /
• pp.81-94
• /
• 2022

• 한국생물공학회:학술대회논문집
• /
• 2005.04a
• /
• pp.31-32
• /
• 2005

Neoagaro-oligosaccharides are produced only by enzymatic degradation of agarose by ${\beta}-agarase.^{1)}$ Neoagaro-oligosaccharides inhibit the growth of bacteria, slow the rate of degradation of starch, are used as low-calorie additives to improve food quality, and have macrophage-stimulating activity. Furthermore, neoagarobiose is a rare reagent that has both moisturizing effect on skin and whitening effect on melanoma $cells.^{2)}$ An agar-degrading marine bacterium was isolated from the sea water at the northeast coast in Cheju island, Korea. The strain was gram negative, aerobic, and motile rod. The 16S rRNA of the strain had the closest match of 98% homology, with that from Agarivorans albus. On the basis of several phenotypic characters and a phylogenetic analysis, this strain was designated Agarivorans sp. JA-1. In solid agar plate, Agarivorans sp. JA-1 produced a diffusible agarase that caused agar softening around the colonies. Agarivorans sp. JA-1 was cultured for 36 hr in marine broth 2216 (Difco, USA) and the supernatant that containing an extracellular ${\beta}-agarase$ was prepared by centrifugation of culture media. The enzyme exhibited relatively strong activity at $40^{\circ}C$ and was stable up to $60^{\circ}C$. Using PCR primers derived from the ${\beta}-agarase$ gene of Vibrio sp., the gene encoding ${\beta}-agarase$ from Agarivorans sp. JA-1 was cloned and sequenced. The structural gene consists of 2931 bp encoding 976 amino acids with a predicted molecular weight of 107,360 Da. The deduced amino acid sequence showed 99% and 34% homology to $agaA^{2)}$ and $agaB^{2)}$ genes for ${\beta}-agarase$ from Vibrio sp., respectively. The expression plasmid for ${\beta}-agarase$ gene of Agarivorans sp. JA-1 is being constructed and the recombinant enzyme will be biochemically characterized.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.10a
• /
• pp.25-25
• /
• 2017

• Herbal Formula Science
• /
• v.29 no.4
• /
• pp.332-332
• /
• 2021

• Herbal Formula Science
• /
• v.29 no.4
• /
• pp.331-331
• /
• 2021

• Journal of Sasang Constitutional Medicine
• /
• v.20 no.3
• /
• pp.21-39
• /
• 2008

1. Objectives The purpose of this study was to find out associations between "HamsanSachon DongyiSuseBowon GaboGubon" and "SangGyoHyeonTo DongyiSuseBowon" 2. Methods A comparative study of authors, colophons, tables of contents and texts of "HamsanSachon DongyiSuseBowon GaboGubon" and "SangGyoHyeonTo DongyiSuseBowon" was made. 3. Results and conclusions 1) There was a close connection between "HamsanSachon DongyiSuseBowon GaboGubon" and "SangGyoHyeonTo DongyiSuseBowon", compared their authors, colophons, tables of contents, texts, and so on. 2) It is supposed that "SaSangInByeonJeungRon" of "HamsanSachon DongyiSuseBowon GaboGubon" was not added any touches and "SaSangInByeonJeungRon" of "DongyiSuseBowon" (GyeongJaBon) was not revised. 3) "DongyiSuseBowon GaboGubon BokWonBon" and text of "SaSangUiHak" shold be amended partially.

• Journal of Life Science
• /
• v.15 no.6 s.73
• /
• pp.884-888
• /
• 2005

An agar-degrading bacterium was isolated from north-eastern sea of Jeju island and cultured in marine agar 2216 media. Biochemical and morphologicl characteristics and 165 rRNA gene revealed that isolated strain was member of Agarivorans genus, and named Agarivorans sp. JA-1. Agarase was produced as growth-related and expressed regardless of agar presence. Optimal pH was 8 at 50 mM Clycine-NaOH buffer, and activity was maximum at $40^{\circ}C$E Enzymatic activity was maintained over $80\%$ at $60^{\circ}C$t and $70\%$ at $80^{\circ}C$ which is thermotolerant. Hence isolated novel Agarivorans sp. JA-1 strain and its beta-agarase could be used for the production of functional oligosaccharide from agar in solution state.

• Journal of the Korean Magnetic Resonance Society
• /
• v.3 no.2
• /
• pp.90-99
• /
• 1999

Doping of boron atoms in graphite has been well known method to increase the discharge capacity as the negative electrode material for lithium secondary battery. Herein, the boron-doped graphites are prepared by mixing 1, 2.5, 5, and 7 wt. % of boron carbide in carbon during the graphitizing process. The structural states of boron in boron-doped graphites are investigated by solid-state 11B NMR spectroscopy. The resonance lines for substitutional boron atoms are identified as the second order quadrupolar powder pattern with the quardrupole coupling constant, QCC = 3.36(2) MHz. The quantitative analysis of 11B NMR spectra with boron-doped graphite has also been performed via simulation.

• Journal of People, Plants, and Environment
• /
• v.22 no.6
• /
• pp.593-600
• /
• 2019

We identified how plants affected by the human behavior of crushing plants respond and what kind of plants responded sensitively. We investigated Lactuca sativa "Gaesebadak", Syneilesis palmata and Peucedanum japonicum as plants that humans use for edible purposes, and Achyranthes japonica and Bidens bipinnata as wild plants that stick to people's clothes and disperse seed. Plants exposed to human breathing air were compared with those exposed to human breathing air after being crushed. Methyl jasmonate (MeJA), a chemical word, was measured using Syft/MS, which detects real-time VOC, and related genes were analyzed. The amount of MeJA of Syneilesis palmata and Peucedanum japonicum as edible plants was greater than that of non-edible plants that disperse seeds using humans. The amount of MeJA ranged from 0.20 ppb to 0.35 ppb when the control group were not exposed to human breathing air. On the other hand, MeJA decreased after increasing for the first hour in human breathing air. Also, MeJA affected by human breathing after crushing plants was higher than that affected by just human breathing air. Peucedanum japonicum showed the most distinctive difference between the treatment with human breathing after crushing plants and the treatment with just human breathing. In addition, the gene activity of JAR1 and JMT increased 3 hours after the treatment with human breathing after crushing plants. Therefore, in the treatment with human breathing after crushing plants, the concentration of MeJA and the activity of related genes showed the same tendency to increase. As a result, the plant that responded sensitively to human behavior was Peucedanum japonicum. Plants released MeJA as a chemical word in the treatment with human breathing air after crushing plants.