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http://dx.doi.org/10.5352/JLS.2005.15.6.884

Isolation and Characterization of a Marine Bacterium Producing Thermotolerant Agarase  

Park Ceun-Tae (Research & University-Industry Cooperation, Pusan National University)
Lee Dong-Ceun (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Kim Nam Young (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Lee Eo-Jin (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Jung Jong-Ceun (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Lee Jae-Hwa (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Heo Moon-Soo (Paculty of Marine Scienes, Cheju National University)
Lee Jung-Hyun (Microbiology Laboratory, Korea Ocean Research and Development Institute)
Kim Sang-Jin (Microbiology Laboratory, Korea Ocean Research and Development Institute)
Lee Sang-Hyeon (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
Publication Information
Journal of Life Science / v.15, no.6, 2005 , pp. 884-888 More about this Journal
Abstract
An agar-degrading bacterium was isolated from north-eastern sea of Jeju island and cultured in marine agar 2216 media. Biochemical and morphologicl characteristics and 165 rRNA gene revealed that isolated strain was member of Agarivorans genus, and named Agarivorans sp. JA-1. Agarase was produced as growth-related and expressed regardless of agar presence. Optimal pH was 8 at 50 mM Clycine-NaOH buffer, and activity was maximum at $40^{\circ}C$E Enzymatic activity was maintained over $80\%$ at $60^{\circ}C$t and $70\%$ at $80^{\circ}C$ which is thermotolerant. Hence isolated novel Agarivorans sp. JA-1 strain and its beta-agarase could be used for the production of functional oligosaccharide from agar in solution state.
Keywords
marine; Agarivorans sp. agarase; thermotorelant enzyme;
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